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Chemical profiling and clustering of Piperaceae revealed by volatilomics analysis Hariani, Sulifah Aprilya; Asyiah, Iis Nur; Prayitno, Trio Ageng
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 9 No. 02 (2024): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v9i02.28

Abstract

The Piperaceae family is one of the aromatic plants that have a distinctive odor and is known to have tremendous benefits in human life.  Piperaceae is used as raw material for medicines, spices, antibacterial, antiviral, and insecticides. Research on the composition of volatile chemical compounds in Piperaceae members is still limited. This study aims to extract volatile chemical compounds in the Piperaceae family and classify Piperaceae members based on their volatile compounds. The type of research is laboratory research. The research samples were leaves of ten species of Piperaceae members, namely Piper cubeba, P. nigrum, P. betle, P. ornatum, P. retrofractum, P. sarmentosum, Peperomia pellucida, Peperomia scandens, Peperomia caperata, and Peperomia maculosa. The research instrument was an observation sheet of SPME-GCMS analysis results. Data in the form of metabo-analysis was obtained from laboratory analysis using SPME-GCMS by producing a comprehensive volatile compound profile of the Piperaceae family and revealing differences between species. The results of SPME-GCMS analysis on the Piperaceae family obtained 197 identified volatile chemical compounds. The largest group of chemical compounds in 10 species of the family consists of sesquiterpene (16.2%), monoterpene (11.7%), sesquiterpenoid (7.1%), alcohol (6.1%), aldehyde (4.6%), terpene (4.1%), terpenoid (4.1%), alkene (3%), fatty acid (2.5%), ketone (2.5%), phenol (2.5%), alkane (2%) and other groups less than 2%.  Characteristics of typical compounds in the genus Peperomia amounted to 10, namely Alpha-pinene, (-)-; Camphene; 2-beta-pinene; l-Limonene; Nonanal; (-)-.beta.-Elemene; alpha.-Copaene; Germacrene D; cis-caryophyllene; Bicycloelemene. In the genus Piper, the variation in the character of volatile chemical compounds is very large, typical characteristics possessed by 6 Piper species are alpha-pinene, (-)-; alpha.-Copaene; alpha.-Humulene; (-)-beta.-Elemene; and trans-Caryophyllene. The study concluded that 197 volatile compounds from 10 Piperaceae species had been identified. The results of this study can be recommended that the Piperaceae family can be optimized for public health.
Identification and analysis of aromatic components in Piper sarmentosum Roxb. from Meru Betiri National Park Hariani, Sulifah Aprilya; Novenda, Ika Lia; Mudakir, Imam; Asyiah, Iis Nur; Pujiastuti, Pujiastuti
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 8 No. 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.1060

Abstract

Piper sarmentosum Roxb or known as mana Karuk is an aromatic plant that has extraordinary potential, namely as a mucus expectorant, its roots are efficacious as a ureter and overcome gallstones. This plant is also used as an asthma drug, a cure for skin diseases such as tinea versicolor, stomach pain, malaria medicine, relieve bone pain and dental pain. The components of aromatic chemical compounds in the leaves and fruits from Meru Betiri National Park (MBNP) have not been identified. This study aimed to identify and analyze the aromatic components of P. sarmentosum leaves and fruits from MBNP using SPME-GCMS. The research method is laboratory research. The research samples and research materials were the leaves and fruit of the P. sarmentosum plant taken in MBNP. The SPME-GCMS tool is used in research to identify and analyze aromatic compounds. The research instrument used was an identification and analysis sheet of aromatic compounds from the P. sarmentosum plant. The data analysis technique uses the SPME-GCMS test. The results of the study showed that there were 46 volatile compounds found in the leaves, while in the fruit there were 29 volatile compounds. The main aromatic compound groups in the leaves and fruits of P. sarmentosum are terpenes, namely monoterpene. The other components consist of organic benzene compounds. P. sarmentosum Roxb. has 46 aromatic compounds in the leaves and 29 other aromatic compounds found in the fruit. Furthermore, research results must be tested in more depth regarding biological activity and mechanism of action through the best methods so that they can be recommended as new drug innovations in society.
Development of E-Module of Science Based on Bondowoso Ethnoscience on Vibration and Wave Material Damayanti, Silvia Qaulina; Supeno, Supeno; Asyiah, Iis Nur
Jurnal Paedagogy Vol. 12 No. 3 (2025): July
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jp.v12i3.15315

Abstract

This study aims to produce Bondowoso ethnoscience-based science e-modules on vibration and wave materials suitable for use in learning activities. The development research uses a 4D model to define, design, develop, and disseminate. Data collection is done through observation, interviews, and expert validation. Interviews were conducted with 8th-grade science teachers and Bondowoso art activists. Validation was carried out by providing validation sheets and developing products for validators. The results of this study indicate that the Bondowoso ethnoscience-based science e-module has a high feasibility level, as evidenced by the average validation score of 89.5%, and the interpretation of validity is very valid. The validity of media feasibility is 87.5%, the validity of material feasibility is 91.7%, and the validity of language feasibility is 89.3%. Based on these results, it can be concluded that the Bondowoso ethnoscience-based science e-module on vibration and wave material is very feasible to use in learning.