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All Journal Dentika Dental Journal Journal of Dentistry Indonesia Indonesian Journal of Biotechnology ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Interdental Jurnal Kedokteran Gigi (IJKG) Narra J Makara Journal of Health Research Cakradonya Dental Journal Odonto dental journal Narra X Journal of Innovative and Creativity Jurnal of Syiah Kuala Dentistry Society SDJ
Endang W Bachtiar
Department Of Oral Biology, Faculty Of Dentistry, Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Isolation and Identification of Indonesian Lactobacillus reuteri strain from Saliva of Young Adults Armelia Sari Widyarman; Stella Pranoto; Citra Fragrantia Theodorea; Endang Winiati Bachtiar; Boy Muchlis Bachtiar
Scientific Dental Journal VOLUME 2, ISSUE 2, MAY 2018
Publisher : Faculty of Dentistry, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/sdj.v2i2.2840

Abstract

Background: Biofilms are involved in a wide variety of microbial infections, including dental caries and periodontitis. The use of probiotics has been a promising prevention and treatment modality with which to combat biofilm-related diseases in the oral cavity. The probiotic Lactobacillus reuteri has been proven to reduce gingivitis and plaque index inside the oral cavity. These bacteria can be found in the digestive system and also in the human oral cavity. Objective: The aim of this study was to identify L. reuteri in the saliva of Indonesian young adults. Methods: Forty saliva samples were collected from 18–24 year-old Indonesian subjects. DNA extraction was performed, and then, the identification of L. reuteri was accomplished using PCR. Six subjects showed positive results. The positive samples were cultured in Rogosa Agar for 24 hours at 37°C anaerobically. Several single colonies were further cultured separately in broth medium before DNA extraction and PCR identification were performed. The four thickest bands were selected for DNA sequencing. Results: An analysis performed using BLAST showed that two of the L. reuteri strains obtained from the Indonesian saliva isolates had 96% (isolate 3.11) and 95% (isolate 5.14) values. This confirmed the presence of new strains based on average nucleotide identity (ANI). The isolate strains of 3.11 and 5.14 have been registered at DDBJ/EMBL/GenBank under the accession number LC382415 and LC382416, respectively. Conclusion: L. reuteri novel strain can be isolated from the saliva of Indonesian young adults.  Further studies involving biochemical tests and phenotypic analysis are needed to better understand these new L. reuteri strains.
Risk of Developing Periodontitis in Patients with Primary Sjogren Syndrome: A Systematic Review Haluanry Santoso; Idson Kamal; Rizki Amanda; Valencia Halim; Endang Winiati Bachtiar
Scientific Dental Journal VOLUME 8, ISSUE 1, JANUARY 2024
Publisher : Faculty of Dentistry, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/sdj.v8i1.19461

Abstract

Primary Sjogren syndrome (pSS) is a chronic autoimmune disorder affecting the exocrine function of the eyes and mouth. Dryness of the mouth and the underlying inflammatory condition may cause alteration of the oral microbiota and connective tissue integrity which may ultimately lead to periodontal disease, such as periodontitis. Studies have shown varying result regarding the association of periodontitis in patients with pSS. This study aims to assess the risk of developing periodontitis in patients with pSS. Literature search was conducted on PubMed, ProQuest, EBSCOHost, and Cochrane databases, with studies evaluating the risk of developing periodontitis in patients with pSS from inception to December 2023 being selected. The Newcastle-Ottawa scale was used in this study to assess the perceived risk of bias of the included studies. This review included one retrospective study and two cross-sectional studies involving 773 patients with pSS. The prevalence of periodontitis in pSS patients is 479 patients (61.9%). Inconsistency of statistical significance is found in regards of relative risk of developing periodontitis. Patients with pSS has an increased relative risk of developing periodontitis. Inconsistency of statistical significance was found between studies.
The Association of CD4+ T Cells Subsets with The Severity of Periodontitis: A Scoping Review Nadiya Nurul; Devi Dwipriastuti; Endang Winiati Bachtiar
Scientific Dental Journal Vol. 8 No. 3 (2025): Scientific Dental Journal
Publisher : Faculty of Dentistry, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/sdj.v8i320423

Abstract

Periodontitis is a persistent inflammatory condition caused by a buildup of oral bacteria that damages the tissues around the teeth. It is defined by the deterioration of these tissues, which can result in tooth loss if not treated. The extent of periodontitis is influenced by various factors, including the host's immune response to bacteria that cause periodontal disease. Periodontal infections induce the differentiation of CD4+ T cells into various subsets, including Th1, Th2, Th17, Tregs, and other subsets. This review aimed to determine if there is an association between different subsets of CD4+ T cells and the severity of periodontitis. The research conducted a scoping review, which utilized the scoping review method to systematically map and analyze the literature results on the subject issue. The article search method employed Scopus, PubMed, and ScienceDirect databases, and a manual search was also conducted using keywords and the Boolean Operators technique. The publications were chosen based on the Preferred Reporting Items for Systematic Review and Meta-Analysis Extension for Scoping Review (PRISMA-ScR) flow, following the established criteria for inclusion and exclusion. As a result, 11 relevant publications were gathered and subsequently examined, focusing on the CD4+ T cell subsets in individuals with periodontitis. The publications show that individuals with periodontitis display a more prominent expression of subgroups of T cells. Moreover, the severity of periodontitis is strongly influenced by the host's immune response, particularly the balance between proinflammatory and anti-inflammatory CD4+ T cell subsets.
Co-Authors Aimee Monica Aimee Monica Alphianti, Likky T. Alwiyansyah, Annisa Nurrahma Amarila Malik Amir, Lisa Rinanda Ananda, Nissia Anggun, Gheta Annisa Qashdina Alghassani Armelia Sari Widyarman Asmarinah Basri A. Gani Basri A.Gani Basril Abbas Benny S Latief Boy Bachtiar Citra Feriana PUTRI Devi Dwipriastuti Dewi Fatma Suniarti Dita Septiani Erik Idrus Erwin Gunawan Eva Fauziah Farida Isroani Haluanry Santoso Heriandi Sutadi Hery Widijanto I. Wayan T. Wibawan Idson Kamal Ikrima, Abidah Ira Tanti Kuijpers-Jagtman, Anne M Lisa Prihastari, Lisa Lubis, Hilda Fitria Megantoro, Aryo Mindya Yuniastuti Mindya Yuniastuty Mindya Yuniastuty Mochamad Fahlevi Rizal Nadiya Nurul Nissia Ananda Nurhadi Ibrahim Nurtami Soedarsono Owen, Kevin Paulina N. Gunawan Peter J Coloe Peter J Coloe, Peter J Peter Smooker Peter Smooker, Peter Prayitno Rachmi Fanani Hakim Rachmi Fanani Hakim Ratna Meidyawati Ratna Ramadhani Refyan, Sarah Athiyyahmaulidya Rintoko, Bimo Rizki Amanda Rizky Aditiya Irwandi, Rizky Rohmah, Devi Kartika Santi Chismirina Sebastian, James Septiwidyati, Tienneke Riana Silvia Sulistiani Sophie Yolanda Stella Pranoto Sulistiani, Silvia Sulistyani, Lilies Dwi Susan Rahayu Theodorea, Citra Fragrantia Tiara Aninditha Valencia Halim Vera Julia Wahyu Sulistiadi Wairooy, Valeria Widita Widyarman, Armelia Sari Yuniarti Soeroso Zaki Mubarak Zinatul Hayati