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Isolation, Characterization and Pathogenicity of Edwardsiella tarda a Causative Disease on Freshwater Fish in Yogyakarta Murwantoko Murwantoko; Eka Diniarti; Triyanto Triyanto
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (565.826 KB) | DOI: 10.22146/jfs.39920

Abstract

Edwarsiella tarda is a cosmopolitan bacterium and is a cause of Edwardsiellosis in various fish species. The bacterial infection causes large losses on aquaculture in Asia, especially Japan. This study was conducted to isolate and characterize E. tarda as causative disease in freshwater fishes, and to determine its pathogenicity to catfish (Pangasius sp.). Bacteria were isolated from kidney of diseased fishes on Tryptone Soya Agar medium. Identification was conducted based on morphological colonies, morphological cells and biochemical tests. Fulfillment of Koch Postulates was done by injecting bacteria intraperitoneally on 7-9 cm fishes at dose of 107 cfu/fish. Pathogenicity test was carried out by intraperitoneal injection at 104, 105, 106, and 107 cfu/fish to 7-9 cm-catfish (Pangasius sp.) and followed by observation of disease signs and mortality every six hours for 7 days. Pathogenicity was determined as Lethal Dosage (LD50) using Dragstedt Behrens method. In this research we have isolated three isolates E. tarda causing disease in fishes. The clinical signs of this disease were lose of pigmentation over the lession, swollen of stomach, haemorhage on fins , small cutaneous lesions, and necrotic on fins area. The LD50 of E. tarda isolate L2, L3, and N3 were 4.64 ± 0.35x105, 1.54 ± 0.07x105, and 1.13 ± 0.13x106 cfu/fish, respectively.
The Effectiveness of Seaweeds as Biofilter for Reducing Wastewater Nutrient and Preventing Water Pollution from Hybrid Grouper Culture Ratih Ida Adharini; Murwantoko Murwantoko; Namastra Probosunu; Riza Yuliratno Setiawan; Tony Budi Satriyo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 2 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i2.28105

Abstract

Highlight ResearchWastewater in hybrid grouper culture should be maintain before resirculate and dispose to aquatic environment to avoid eutrophication.Seaweed that is used for biofilter must be observed for its suitability, performance and effectivity.Ulva sp. has the best effectiveness to reduce nutrient in wastewater of hybrid grouper aquaculture by absorbing then stored into thallus.Ulva sp. is proven has the best SGR and adaptability in wastewater of hybrid grouper culture.AbstractWastewater generated from hybrid grouper culture needs to be managed to improve water quality before being recirculated, or discharged in the aquatic environment. Seaweed biofilter has been proposed in wastewater treatment technology for marine fish farming. This study aimed at comparing which of these species i.e. Ulva sp., Sargassum sp., Gelidium sp., and Dictyota sp.  work best to absorb nutrient wastewater hybrid grouper culture. This research utilized these seaweed as treatments and controls with three replications using Completely Randomized Design. A statistical analysis was conducted to investigate the significant differences in the nutrient absorptions among various seaweed species by using ANOVA and least significant difference. Non-parametric tests namely Kruskal-Walis, Mann-Whitney, and t-test were used with confidence interval of 95%. The results revealed that Ulva sp. has the best ability to reduce the Nitrogen 80%, while Dictyota able to reduce  Phosphor 88% by Dictyota sp. generated from wastewater of hybrid grouper culture. While the highest absorbtion of Total Kjeldahl Nitrogen (104%) and phosphate (182%) that stored in thallus were performed by Ulva sp. Ulva sp. had the best performance and highest growth rate (1.9% d-1) as biofilter in hybrid grouper cultivation than other species.
Morphometric and Meristic Characterization of Hairtails Trichiurus lepturus Linnaeus, 1758 (Scombriformes: Trichiuridae) from the Northern Coast of Java, Indonesia Adhimas Febri Aryantojati; Murwatoko Murwantoko; Eko Setyobudi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i1.31443

Abstract

Highlight ResearchThe most significant morphological variation was the snout length and caudal peduncleT. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same populationT. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species AbstractHairtails (Trichiurus spp.) are economically important fisheries resources and have become an export commodity. In Indonesia, there are several hairtail species; however, each species distribution is not yet certainly known. This study aimed to identify and determine the morphometric and meristic character of the hairtail from the northern coast of Java, Indonesia. Fish specimens were obtained from fishermen catches in the north coast of Pati and Demak (Central Java) and the northern coast Cirebon (West Java), which captures the fish in less than one-week fishing operation. Fish samples were grouped based on morphological similarities identified in previously published works. After that, 22 morphometric and four meristic characters were measured and counted. Principal Component Analysis and Discriminant Analysis were used to analyze morphometric data, while the meristic data were compared to the available published works. The hairtail caught at the northern coast of Java was long, flat, silvery in color, and had a sagittal crest, with the fin formula being D.III.125-134, P.I.10, and A.I.98-104. According to the morphological and meristic characters, the hairtail caught at Java's northern coast was identified as Trichiurus lepturus. The most significant morphological variation was the snout length and caudal peduncle. The PCA and scattergram analyses showed that T. lepturus from the coast of Pati and Cirebon have a high morphometric character resemblance and possibly are members of the same population.  T. lepturus in Demak waters formed a separate population group from T. lepturus from the coast of Pati and Cirebon, however still in the same species.
Food Habits of Red Snapper Lutjanus malabaricus in Pinrang Waters L Rapi, Nuraeni; Tri Hidayani, Mesalina; Djumanto, Djumanto; Murwantoko, Murwantoko; Alifia, Frida
Agrikan Jurnal Agribisnis Perikanan Vol. 15 No. 1 (2022): Agrikan: Jurnal Agribisnis Perikanan
Publisher : Fakultas Pertanian, Universitas Muhammadiyah Maluku Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1281.101 KB) | DOI: 10.52046/agrikan.v15i1.1211

Abstract

Pinrang Regency is included in the scope of Indonesian waters which has the potential to catch red snapper. Snapper is an export commodity that has a high market price with a selling value of around Rp. 70,000 / kg. The aim is to determine the size structure, gonad maturity and sex ratio. This research was conducted from March to July 2019 in the waters of Pinrang Regency. The observed aspects are size structure, gonad maturity and sex ratio. The results obtained as many as 532 fishes, of which 226 males and 306 females. The total length ranges from 19.5 to 69.5 cm with the sex ratio in a balanced state. Gonadal maturity is 36% under immature conditions and 64% are mature gonads. The size of the first time maturity is 29 cm in males and 37 cm in females.
Size Distribution and Length-Weight Relationship of Red Snapper (Lutjanus malabaricus) in Pinrang Waters Regency of Pinrang Rapi, Nuraeni L; Hidayani, Mesalina Tri; Djumanto, Djumanto; Murwantoko, Murwantoko
Agrikan: Jurnal Agribisnis Perikanan Vol 12, No 2 (2019)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.agrikan.12.2.317-321

Abstract

Fisheries activities red snapper in Pinrang waters is considered to be a small scale and caught with that variety of types instrument catch fish (multi gears) one of them is bubu fishing gear. Now, the level of the utilization of red snapper is believed to have been experience symptoms of over fishing due to the high pressure in the area. The study aims to analyze the size structure and length-weight correlation of red snapper (Lutjanus sp). The study was conducted during 5 months of period, starting from March to July 2019 in Pinrang waters, Pinrang District. The sample of the fish obtained by fishermen using ‘Bubu’ fishing gear. The results showed that the total length structure of males and females of the red snapper was ranging from 32 - 57 cm, while the weight structure of males and females of the red snapper had a wide range from 500 to 2500 grams. The total sample of the red snapper is 163 divided into 68 males and 95 females. The growth pattern of red snapper can be categorized in a negative allometric type with a value of b = 2.3328 which means that the length increased faster than the weight gain.
DINAMIKA POPULASI IKAN KAKAP MERAH (Lutjanus malabaricus) di PERAIRAN PINRANG Rapi, Nuraeni L; Djumanto, Djumanto; Murwantoko, Murwantoko
Jurnal Penelitian Perikanan Indonesia Vol 28, No 4 (2022): (Desember) 2022
Publisher : Politeknik Kelautan dan Perikanan Karawang, BRSDM KP.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jppi.28.4.2022.177-185

Abstract

Ikan kakap merah merupakan salah satu komoditas perikanan yang bernilai tinggi sebagai ikan konsumsi. Pengetahuan sebaran panjang, umur, pertumbuhan, dan mortalitas digunakan untuk mengetahui kondisi stok ikan. Penelitian ini bertujuan untuk menganalisis pertumbuhan, mortalitas, laju eksploitasi dan pola rekrutmen ikan kakap merah (Lutjanus malabaricus). Sampling dilakukan tiap bulan sekali dari April 2019 sampai Maret 2020 di perairan Kabupaten Pinrang. Contoh ikan diperoleh dari hasil tangkapan nelayan gill net,rawai dasar dan bubu. Tiap contoh ikan dilakukan pengukuran panjang menggunakan penggaris dengan ketelitian 0,1 mm. Data frekuensi panjang dianalisis menggunakan paket program FiSAT II untuk mengetahui sebaran panjang, laju pertumbuhan, mortalitas,tingkat eksploitasi dan pola rekrutmen. Total sampel ikan yang dikumpulkan selama penelitian sebanyak 916 ekor. Nilai L∞ sebesar 72,95 cm, nilai K sebesar 0,41/tahun dan nilai t0 sebesar-0,28. Laju mortalitas total sebesar 1,21, mortalitas alami (M) sebesar 0,5, mortalitas penangkapan (F) sebesar 0,71, dan Tingkat eksploitasi (E) sebesar0,59. Rekrutmen berlangsung tiap bulan dengan persentase tertinggi pada akhir musim hujan, puncak musim kemarau dan awal musim hujan. Perlu pengaturan penangkapan tentang ukuran mata pancing dan mata jaring agar tingkat eksploitasi sumberdaya ikan kakap merah berkesinambungan Red snapper is a fisheries commodity with high value as a potential export. Knowledge of the distribution of length, age, growth, and mortality is used to determine fish stocks' condition. This study analyzes the growth, mortality, exploitation rate, and recruitment pattern of red snapper (Lutjanus malabaricus). Sampling has been carried out every month from April 2019 to March 2020 in Pinrang Regency waters. Fish samples were obtained from the catch of gill net fishermen, bottom longlines, and traps. Each fish sample is measured using a ruler to a length of 0.1 mm. Long frequency data were analyzed using the FiSAT II program package to determine the length distribution, growth rate, mortality, exploitation rate, and recruitment patterns. The total fish samples collected during the study were 916 individuals. The L∞ value is 72.95 cm, the K value is 0.41 / year, and the t0 value is -0.28. The total mortality rate is 1.21, natural mortality (M) is 0.5, fishing mortality (F) is 0.71, and the exploitation rate (E) is 0.59. Recruitment occurs every month with the highest percentage at the end of the rainy season, the dry season's peak, and the beginning of the rainy season. Fishing arrangements are needed about size hook and mesh size  so that the level of exploitation of red snapper resources is sustainable.
Record of Acanthocephala on Shortfin Scad (Decapterus macrosoma Bleeker, 1851) from the Southern Coast of the Special Region of Yogyakarta, Indonesia Kolokaring, Priscilla Yolanda Pramesti; Syarifah, Rizka Fauziana; Hidayat, Hanindya Fairuzia; Astuti, Niken; Murwantoko, Murwantoko; Setyobudi, Eko
Journal Omni-Akuatika Vol 21, No 1 (2025): Omni-Akuatika May (in progress)
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2025.21.1.1215

Abstract

Acanthocephala is a parasite commonly found in the digestive tract of fish. This study aimed to determine the prevalence and mean intensity of Acanthocephala infecting the shortfin scad (Decapterus macrosoma) caught from the southern coast of Yogyakarta and to identify those parasites by morphological approach. A total of 221 samples of fish were obtained from local fishermen operating in fishing grounds along the southern coast of the Indian Ocean in the Special Region of Yogyakarta. The fish samples were measured for total length (TL) and weight and dissected to examine for the presence of Acanthocephala. Parasite samples were collected from the digestive tract of shortfin scad and preserved in absolute ethanol for further identification. Data analysis focused on calculating prevalence and mean intensity of infection. The results showed that the prevalence of Acanthocephala infection was 16.3%, with a mean intensity of 1.9 larvae/host. Both the prevalence and the mean intensity tended to increase with increasing fish length. Most of the shortfin scad (75%) was infected with Acanthocephala at a relatively low intensity (1-2 larvae/host). The highest intensity of Acanthocephala infection was 13 larvae/host and was only found in one fish. Morphological identification indicated that the genus infecting shortfin scad was Acanthocephalus (Koelreuther, 1771).Keywords: Acanthocephalus, biological tag, morphology, parasite, prevalence
Co-Authors . Triyanto Adhimas Febri Aryantojati Alifia, Frida Alim Isnansetyo Alim Isnansetyo ALIM ISNANSETYO Anshory, Lutfi Aushia Tanzih Al Haq Bambang Triyatmo Cahya Kurnia Fusiyanto Ch. Retna Handayani Chio Oka Chio Oka, Chio Christina Retna Handayani Christina Retna Handayani, Christina Retna Condro, Sri Laksono Dewi Nur'aeni Setyowati Dewi Susanti Dimas A. Pamungkas Djumanto Djumanto Djumanto Djumanto Dwi Lantiani Eka Diniarti Eko Setyobudi Eko Suyanto Ekowati Chasanah EKOWATI CHASANAH Gemi Triastutik Hidayani, Mesalina Tri Hidayat, Hanindya Fairuzia INDAH ISTIQOMAH JAKA WIDADA Kamiso H. Nitimulyo Kolokaring, Priscilla Yolanda Pramesti L Rapi, Nuraeni Lusiana, Lusiana Lutfi Anshory Lutfi Anshory Masashi Kawaichi Masashi Kawaichi Masashi Kawaichi, Masashi Melki Melki Mesalina Tri Hidayani Muhammad Nursid MUHAMMAD NURSID Namastra Probosunu Nani S. Rahayu Nastiti Wijayanti Ngurah S. Yasa Ngurah Sedana Yasa Niken Astuti, Niken Niken S. N. Handayani Niken S.N. Handayani Nuraeni L Rapi Nuraeni L Rapi Nuraeni L Rapi Praditha Novianingrum Rarastoeti Pratiwi Rarastoeti Pratiwi Ratih Ida Adharini Ristyana Dewi Hernawati Riza Yuliratno Setiawan Roosita Diana Rozi Rozi Sitty Ainsyah Habibie Sri Rejeki Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sutopo A. Wibowo Syarifah, Rizka Fauziana T. Trijoko Toni Kuswoyo Tony Budi Satriyo Tri Hidayani, Mesalina Triastutik, Gemi Triyanto . Triyanto Triyanto Triyanto Triyanto Yani Lestari Nuraini Yani Lestari Nuraini, Yani Lestari Yasa, Ngurah Sedana Yasir Saifur Rahman Yuliana S. Rahayu Zafran, Zafran