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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Journal of Applied Veterinary Science and Technology Buletin Veteriner Udayana Advances in Tropical Biodiversity and Environmental Sciences

I Wayan Bebas

Laboratotium Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 241740

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Pengaruh Berbagai Konsentrasi Dimethylsulfoxide terhadap Kualitas Semen Beku Ayam Hutan Hijau Post Thawing Wayan Bebas; DND Indira Laksmi
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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The process of freezing and thawing on semen can lead to physical stress, often called cold shock, and couses the structural and biochemical damage that affecting cell function and ultimately lead to the death of the cell The aim of this study was to know the effect of the addition of various concentrations of dimethylsulfoxide (DMSO) as the intracellular cryoprotectant in phosphate yolk diluent on the post thowing quality of the green jungle fowl semen. The study used eight green jungle fowl semens which were collected with massage techniques. Semen was evaluated macroscopically and microscopically. Good quality semen was diluted with phosphate yolk which was added four different concentration of DMSO, namely 4%, 6%, 8%, and 10%. Semen was then filled and sealed in a mini straw (0.25 mL) with the concentration of 150.106 cells, and equilibrated at 4oC for 4 hours. The semen freezing was processed using conventional method. Evaluation was performed on post thawing semen. The evaluation of semen quality included the progressive motility and plasma membrane intact. Data were analyzed by analysis of variance. If there were any significant differences, the data were futher analyzed by Duncan test. The results showed that addition of DMSO concentration of 6% has resulted the progressive motility and intact plasma membrane higher significantly (P

The Effect of Soyabean Extender on Viability and DNA Integrity of Kintamani Dog Sperm on Cold Storage I Made Yoga Windu Pradana; Wayan Bebas; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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The objective of this study was to evaluate the viability and integrity of canine semen diluted in egg yolk glucose-citrate and Tris-citrate-glucose-soya bean. Semen was collected by manual manipulation from an apparently healthy kintamani dog stud. The semen was subjected to gross and microscopic examination to determine its viability following dilution in different extenders. Only semen with motility rate of 60% or higher was used in this study. Semen samples were diluted in Tris-citrate-glucose-soya bean and egg yolk glucose-citrate extenders at two levels of dilution; sperm to extenders ratio of 1:2 and 1:3. Following semen dilution, evaluations were made on sperm motility, percentage of live sperm, and DNA integrity at 0 hour, 3 hours, and 6 hours under 5oC of storage. Data was analyzed by multivariate analysis of variance (MANOVA).The present results showed a high significant different (p<0.01) in sperm motility, percentage of live and DNA integrity between treatment with egg yolk glucose-citrate and Tris-citrate-glucose-soya bean. The result also showed that percentage of live sperm and DNA integrity of semen at 1:2 dilution difference significantly (p<0.01) to that of 1:3 dilution. The sperm viability and integrity were significantly difference (p<0,01) between the duration of storage at 5°C. Thus, the current study indicated that Tris-citrate-glucose- soya bean extenders was sufficient to maintain motility, viability, and DNA integrity of kintamani dog spermatozoa during storage at 5 oC. Further research should be conducted to evaluate the fertility of spermatozoa following dilution with these extenders.

Penerapan Teknologi Reproduksi untuk Mempertahankan Ketersediaan Ayam Upakara di Bali I N. Suartha; I W Bebas; I. G.N.K. Mahardika
Buletin Udayana Mengabdi Vol 15 No 1 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Chicken is important part in Balinese Hindu ceremony. Chicken were used as ceremony are chicken with black feather (siap selem), red feather (siap biing), white feather (siap putih) that a white feather chicken with white colour feet, yellow feather (siap putih siungan), that a white feather chicken with yellow colour feet, and chicken with mixed colour of those four colour (siap brumbun). Limitations of technology owned by community makes the chicken’s breeding doesn’t provide economic added value. The purpose of this service is to provide innovation of artificial insemination technology of chicken production for ceremony. The Innovation is given through counseling and workshops . The results showed that people knowledge about disease and breeding management was increased. The farmer have been able to do artificial insemination and vaccination in chicken. The participant were very enthusiastic in following the activities and understand the disease and how to prevent diseases in chickens. These skills are need to trained constantly to find the comfortable thechnique. The conclusions is farmers have been able to perform artificial insemination in chicken.

Penambahan Astaxanthin pada Pengencer Kuning Telur Berbagai Jenis Unggas Dapat Memproteksi Semen Babi Selama Penyimpanan (THE ADDITION OF ASTAXANTHIN ON SPERM DILUENTS PHOSPHATE EGGYOLK OF VARIOUS POULTRY CAN PROTECT QUALITY OF PIG SPERM DURING STORAGE) Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was conducted to formulate semen diluent of pigswith a better quality, cheap and easy toprepare using egg yolk of various poultries such as chickens, ducks and quails in combinationwithastaxanthin, a potent antioxidant. The research design used was a completely randomized factorialdesign with three different types of sperm diluents and four levels of astaxanthin concentration. Spermdiluents used were phosphate duck egg yolk, phosphate quail egg yolk and yolks phosphate supplementedrespectivelywith 0,002%, 0,004% and 0,008%astaxanthine. The treated sperm were strored at 5oC for 48hours. The sperm qualities were examined for progressive motility, spermatozoa abnormalities, viabilityand plasma membrane integrity. The result showed that sperm diluents of using duck egg yolk phosphatein combination with 0.002% astaxanthinresulted in the highest progressive motility, viability and plasmamembrane intact while abnormalities spermatozoa is lowest. It can be concluded that phosphate duck eggyolk sperm diluents with the addition of 0,002% astaxanthinappeared to be able to maintain the qualityof pig sperm stored at 5oC for 48 hours.

LAKTOSA-ASTAXANTHIN MENINGKATKAN KUALITAS SEMEN BEKU KAMBING GEMBRONG DALAM UPAYA KONSERVASI Wayan Bebas; Wayan Gorda; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Tjok Gede Oka Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research was conducted to produce a formula of diluent for the manufacture of frozen semen of Gembrong goat. Yolk phosphate was used as a basic diluent with the addition of anti-cold shock, antioxidants and combination of anti-cold shock-antioxidant. The research design used a completely randomized design with three kinds of treatments; T1: the addition of lactose 0.6% (anti-cold shock), T2: the addition of astaxanthin 0.004% (antioxidant), and T3: a combination of 0.6% lactose-Astaxanthin 0.004% (combination of anti-cold shock and antioxidants). The addition of DMSO 6% was used in each treatment as intracellular cryo protectants. The freezing process was done with the gradual freezing with conventional techniques. Examination of the quality of semen by thawing prior included progressive motility, viability, and abnormalities. The results showed that anticoldshock-antioxidant combination (0.6% lactose -Astaxanthin 0.004%) produced the best semen quality with progressive motility, viability, and less abnormalities as follow 44.00 + 3.46%, 59.00 + 1.85%, and 14.00 + 0.76% respectively. It was concluded that the addition of a combination of 0.6% lactose-Astaxanthin 0.004% on the phosphate yolk diluent with 6% DMSO as intracellular produced best quality of frozen goat Gembrong semen that suitable for use in artificial insemination and in vitro fertilization.

PREGNANT MARES SERUM GONADOTROPHIN INCREASES NUMBER OF EGGS AND ACCELERATES LAYING PERIOD OF BALI DUCK WITH DELAYED EGG PRODUCTION Made Kota Budiasa; Wayan Bebas
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was carried out to determine the effect of Pregnant Mares Serum Gonadotrophin (PMSG) on the weigth of ovary, the number of developing follicles and the onset egg production in ducks with delayed egg production. In this study, as many as 40 Bali ducks were used and they were divided into 4 groups in a completelty randomized design i.e. control group (TO) treated with NaCl 0,9%, group T1 treated with PMSG dose 5 IU per duck, group T2 treated with PMSG dose 10 IU per ducks and group T3 treated with PMSG dose 20 IU per duck intramuscularly. The weight of ovary increased significantly with the increasing dose of PMSG, i.e dose 0 IU (21,04 + 3,90 gram), dose 5 IU (25,40 + 5,31 gram), dose 10 IU (49,52 + 5,05 gram) and dose 20 IU (59,30 + 4,66 gram). The number of developing folilicles also increased significantly with the increasing dose of PMSG, i.e dose 0 IU (1,4 + 0,5 follicles), dose 5 IU (2,4 + 1,34 follicles), dose 10 IU (5,8+2,07 follicles) and dose 20 IU (8,6+1,67 follicles). In addition, the onset of egg production was shortened significantly with the increasing dose of PMSG, i.e. dose 0 IU (19,0+1,58 days), dose 5 IU (16,8+1,3 days), dose 10 IU (14,2+1,92 days) and dose 20 IU (9,6+14 days). PMSG treatment was proven to increase the egg production capability of ducks with delayed egg production.

Kadar Krioprotektan Gliserol dan Dimethylsulfoxide Terbaik pada Pengencer Astaxanthin Fosfat Kuning Telur Bebek Terhadap Kualitas Semen Beku Babi Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study to examine the addition of cryoprotectant glycerol and dimethylsulfoxide (DMSO) in astaxanthin phosphate duck egg yolk on the quality of frozen boar semen. Each of the cryoprotectants distinguished its concentration ie : 4%, 6%, 8%, 10%, and 12% for freezing of semen. Semen was collected from one Landrace boar then performed macroscopically and microscopically. Semen was divided into 10 tubes each diluted with the duck egg yolk astaxanthin phosphate extender diluent then stored at 20oC for 2 hours, centrifugation for 15 minutes (2000 rpm) then taken the pellet with 1 ml of supernatant. The pellets were diluted with astaxanthin phosphate duck egg yolk extender containing various concentration (4, 6, 8, 10, and 12%). Semen was packed with 0.5 mL straw and performed equilibration for 2 hours (4oC). do aeration above liquid N2 vapor for 10 minutes, then dipped in liquid N2. Semen was stored in liquid nitrogen container for 24 hours , and then quality of spermatozoa was evaluated post thawing. The results of the study showed that addition of cryoprotectan glycerol and DMSO were no difference significant (p>0,05) on the quality of frozen semen, but cryoprotectant concentrations (4%, 6%, 8%, 10%, and 12%) significantly affected (p <0.05) the quality of frozen semen. Glycerol with a concentration of 4% produces the best quality frozen semen, whereas DMSO with a concentration of 6% produces the best quality frozen semen.

Penambahan Bovine Serum Albumin pada Beltsville Thawing Solution Dapat Mempertahankan Kualitas Semen Babi yang Disimpan pada 15°C (ADDITION OF BOVINE SERUM ALBUMIN TO BELTSVILLE THAWING SOLUTION COULD MAINTAIN QUALITY OF PIG SEMEN STORED AT 15OC) Wayan Bebas; Wayan Gorda
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aims to maintain the quality of pig semen for longer during storage at 15oC, in an effort to support artificial insemination programs with the addition of Bovine Serum albumin (BSA) to diluent Beltsville Thawing Solution (BTS). This study uses a completely randomized design with five treatment groups, each To = semen was diluted with BTS without the addition of BSA ; T1 = with the addition of 5 mg BSA/mL diluent; T2 = with the addition of 10 mg BSA/mL diluent; T3 = with the addition of 15 mg BSA/mL diluent; T4 = with the addition of 20 mg BSA/mL diluent. Each treatment was repeated five times so that the number of samples used was twenty-five. The diluted cement is stored at 15oC for 72 hours then observing the quality of cement includes: progressive motility, dead spermatozoa, abnormalities, and intact plasma membranes. The data obtained were analyzed by analysis of variance, if there were differences followed by Duncan’s test. The results showed, addition of BSA concentration of 10 mg/mL and 15 mg/mL of diluent gives the same effect on the quality of cement during storage and significantly better (p <0.05) when compared to the addition of 0 mg/mL, 5 mg/mL and 20 mg/mL diluents. It can be concluded, the addition of BSA 10 mg/mL BTS diluents can maintain the most optimal quality of pig semen against progressive motility, dead spermatozoa, abnormalities and intact plasma membranes.

Viabilitas Spermatozoa Ayam Hutan Hijau dalam Pengencer Posfat Kuning Telur Ditambah Laktosa pada Penyimpanan 5oC (GREEN JUNGGLE FOWL SPERM VIABILITY IN EGG YOLK PHOSFAT DILUENTS CONTAINING LACTOSE STORAGED AT 5OC) Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objective of this study was to determine the addition of lactose on egg yolk phosphate diluentsstored at 5°C to the plasma membrane intact, acrosome membranes intact of green jungle fowlspermatozoa, fertility and hatchability of chicken eggs. This study used completely randomized designwith three treatment groups. Treatment I (T0): egg yolk phosphate diluents only (control), treatment II(T1): egg yolk phosphate diluents + lactose 0.3%, and treatment III (T2): egg yolk phosphate diluents +lactose 0.6 %. Each group was replicated nine times. Semen was collected from eight green jungle fowl andevaluated both macroscopically and microscopically. Semen was diluted with each treatment with a singleinsemination dose (0.5 mL) with a concentration of 150 million progressively motile cells. Once diluted,semen was stored at 3-5°C for 48 hours and then was observed for the plasma membrane of intact,acrosome membrane of intact, and some semen were inseminated on local female chicken village forfertility, and hatchability eggs observation. The results showed that the addition of lactose 0.3% and 0.6%providing significant increase (P <0.05) on plasma membrane intact, acrosome membrane intact, fertilityand egg hatchability when compared to controls. However the addition of 0.3% and 0.6% lactose did notshowed a significant effect (P> 0.05). This study concluded that the addition of lactose on egg yolk phosphatediluents for storing green jungle fowl spermatozoa at 3-5oC for 48 hours can improve the plasma membraneintact, acrosome membranes intact, fertility and hatchability of chicken eggs.

Surgical Removal of Air Rifle Projectiles in Long-Tailed Macaques (Macaca fascicularis) in the Ubud Monkey Forest Sewoyo, Palagan Senopati; Wirata, I Wayan; Gorda, I Wayan; Wardhita, Anak Agung Gde Jaya; Bebas, I Wayan; Soma, I Gede; Putra, I Gusti Agung Arta; Wandia, I Nengah; Brotcorne, Fany
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.158-162

Background: Long-tailed macaques (Macaca fascicularis) frequently share their habitat with humans, leading to conflicts, especially when habitat disturbances force them to exploit human food sources. In response, humans sometimes use air rifles to deter these animals. Preventive measures such as sterilization programs, habitat restoration, and public education about responsible interactions with macaques are essential to reduce the occurrence of such incidents and promote coexistence. Purpose: This report describes the anesthesia and surgical management of gunshot wounds in long-tailed macaques. Case(s): Two adult free-ranging male long-tailed macaques in the Ubud Monkey Forest, Bali, Indonesia, were presented with gunshot wounds on the inguinal region and right caudal pelvic area, respectively. These macaques were captured and anesthetized for a scheduled vasectomy. A detailed clinical examination indicated that the projectiles were lodged in the subcutaneous tissue. Based on their shape and size, the projectiles were suspected to be 0.177 caliber (4.5 mm) air rifle pellets. Case Management: Surgical removal was performed to extract the air rifle pellets. Preoperatively, the macaques received amoxicillin (15 mg/kg BW, IM), ketorolac (2.5 mg/kg BW, IM), and Visine® eye lubricant. Propofol was used for anesthetic maintenance (1 mg/kg BW, IV). Following pellet removal, the wound was sutured with monofilament absorbable poliglecaprone 25, and the planned vasectomy was conducted using the bilateral incision method. Postoperatively, the macaques were administered atipamezole (0.02 mg/kg BW, IM) for anesthesia reversal, placed in recovery cages in lateral recumbency, and closely monitored. Conclusion: Once the macaques showed clear signs of recovery from anesthesia, they were transported back to the capture site and released into their original group.

Co-Authors Aerawata, I Wayan Gede Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Annisya Syarifuddin Apriliana, Kadek Soma Apriliani, Karolina Artha Guntur Wahana Brotcorne, Fany Budi, Josephine Aurora David Zulkarnain, David Desak Nyoman Dewi Indira Laksmi DEVI INDRAWATI DND Indira Laksmi Drystiana Yessi Ayu Lesmono Dyonesia Dimitri Maya Mayesta EVA AGUSTINA SIAHAAN Geovany Larastiyani Buyona Haq, Nurul Ikhsanul Hendiyani, Melia Heros, Made Novi Lerianti I Gede Soma I Gusti Agung Arta Putra I Gusti Ngurah Agung Dolly Octa I Gusti Ngurah Bagus Tri Laksana, I Gusti Ngurah Bagus I Gusti Ngurah Bagus Trilaksana I Gusti Ngurah Kade Mahardika I Ketut Anom Dada I Ketut Puja I Ketut Sumadi I Made Agus Suryanatha I Made Damriyasa I Made Hermadi Putra I Made Yoga Windu Pradana i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Wayan Gorda I Wayan Piraksa I Wayan Wirata Ika Yuni Astutik Jian Rindawidya Pambudi, Jian Rindawidya juliantari, Ni Komang Ade Komang Gita Permana Kusuma, Putu Wijaya LUH PUTU DHATU HANNY ADNANI MADE KOTA BUDIASA Ni Komang Asni Ni Made Dwi Permata Sari Ni Nyoman Werdi Susari NUR ANIS SAFITRI Pandia, Yoga Mahendra Pradnyani, Gusti Ayu Putu Indira Priharyanthi, Luh Komang Ayu Puteri Putri, Priscilla Mariani Sariyono Rahel Situmorang Ribka Natasia Abel Rinna Noviyati Ndun Sandra, Arista Novi Sewoyo, Palagan Senopati Tani Bina, Elyas Herybertus Teja, Dewa Ngakan Gede Surya Tjok Gde Oka Pemayun Tjok Gede Oka Pemayun Tjok Gede Oka Pemayun, Tjok Gede Oka Toli, I Putu Gde Pradnya Nolan Wahyu Kusuma Atmaja Widiastuti, Wayan Arni Widodo Cipto Subagyo

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