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All Journal Medical Journal of Indonesia Indonesian Journal of Urology Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Narra J JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Griya Widya: Journal of Sexual and Reproductive Health Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute
Ponco Birowo
Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Other

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Effect of pH and neutrophil count on the motility and persistence of spermatozoa in the vagina of candidiasis rat models Iswara, Raja AW.; Hestiantoro, Andon; Budiningsih, Yuli; Werdhani, Retno A.; Birowo, Ponco; Wuyung, Puspita E.; Afandi, Dedi
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.823

Abstract

Sexual violence is a global issue affecting individuals regardless of their relationship to the perpetrator or the setting. Microscopic examination of spermatozoa from vaginal swabs is crucial in investigating cases of sexual intercourse to determine the time of the crime. Factors such as vaginal pH and neutrophil count influence the motility and persistence of spermatozoa in the vagina, particularly in conditions like candidiasis, highlighting the need for further research in this area. This study aimed to determine the effect of pH and neutrophil count on the motility and persistence of spermatozoa in the vagina with candidiasis. An experimental study was conducted using white rats (Rattus norvegicus) of the Wistar strain, with four male rats providing spermatozoa samples and 32 female rats receiving treatment. The female rats were divided into two groups: the normal group and the candidiasis model group. In both groups, the female rats were given vaginal insemination of spermatozoa. Variables measured included pH, neutrophil count, motility, and persistence of spermatozoa in the vagina. Data were analyzed using the Mann-Whitney test, followed by the Spearman correlation test. The findings revealed that spermatozoa motility lasted up to three minutes in normal rats, whereas in the candidiasis model, it was reduced to two minutes. Additionally, spermatozoa persistence in the vagina lasted up to six days in the normal group compared to up to three days in the candidiasis model. There were significant differences in pH, neutrophil count, motility, and persistence of spermatozoa in the vagina between the normal group and the candidiasis model (all had p<0.001). There was a correlation between pH and neutrophil count with the motility and persistence of spermatozoa in the rat’s vagina (p<0.001). In conclusion, vaginal pH and neutrophil count influence the motility and persistence of spermatozoa in the vagina of candidiasis rat models.
Impact of semen insemination on the vaginal microbiome profile of candidiasis rat model: A preliminary forensic study on sexual violence evidence Iswara, Raja AFW.; Hestiantoro, Andon; Budiningsih, Yuli; Werdhani, Retno A.; Birowo, Ponco; Wuyung, Puspita E.; Fadilah, Fadilah; Afandi, Dedi
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1256

Abstract

Sexual violence, including sexual intercourse, can occur in women experiencing vaginal discharge, particularly in cases of vaginal candidiasis. In candidiasis, the vaginal microbiome undergoes changes that could serve as a diagnostic indicator or as evidence of sexual activity.  The aim of this study was to assess the effects of semen insemination on the vaginal microbiome profile of candidiasis rats and to determine its forensic investigations in cases of sexual violence.  An experimental study was carried out using Wistar strain rats (Rattus norvegicus), consisting of four male rats (for spermatozoa donors) and twenty-four female rats. The female rats were divided into four groups: normal condition (control), normal condition post-semen insemination, candidiasis rats, and candidiasis rat post-semen insemination. Vaginal microbiome profiles were examined for each group, using alpha diversity (Chao 1, Shannon, Simpson, and Faith PD indices) and beta diversity (Bray Curtis, Jaccard, Unweighted Unifrac and Weighted Unifrac indices). Data were analyzed using the Kruskal-Wallis test for alpha diversity and the PERMANOVA test for beta diversity. The vaginal microbiome profiles of normal and candidiasis rats showed no significant differences (p>0.05). In candidiasis rats, the microbiome predominantly consisted of the Eukaryota kingdom, particularly Candida albicans. Semen insemination did not significantly affect the vaginal microbiome profile of candidiasis rats in the short term (p>0.05). However, the study highlights that the presence of Eschericia-Shigella, Roseomonas, and Archaea in the vaginal microbiome post-semen insemination potentially serves as an indicator of infection or sexual activity in forensic contexts.
Co-Authors Achmadsyah, Armand Adianti Khadijah, Adianti Agus Rizal Ardy Hariandy Hamid, Agus Rizal Ardy Hariandy Akmal Taher Andon Hestiantoro Ari Astram, Ari Ari Basukarno, Ari Aria Kekalih Ariyati, Ira Arry Rodjani Asmarinah Biddulth Sujana, Biddulth Brodjonegoro, Sakti R Budi Wiweko C Susilo, C Chaidir A. Mochtar CHAIDIR MOCHTAR, CHAIDIR charles johanes, charles Danarto, Raden Dedi Afandi Djoko Rahardjo donny eka putra, donny eka Dyandra Parikesit, Dyandra Endrika Noviandrini, Endrika Fadilah Fadilah, Fadilah Fajriani, Rosalina Fakhri Rahman, Fakhri Febriyani Ferry Safriadi Fiastuti Witjaksono Gede Wirya Kusuma Duarsa Gerhard Reinaldi Situmorang, Gerhard Reinaldi Gunawan, I Putu Gde Fredy Hakim, Rizky Lukman Harahap, Alida Roswita Hariwibowo, Rinto Hendri, Ahmad Z Heri Wibowo Hernawan Rahmat Muharia, Bayu Hery Tiera I Putu Gde Sanjaya, I Putu Ikhlas A. Bramono, Ikhlas A. Indah S. Widyahening Indrawarman Soeroharjo Irfan Wahyudi Isaac A. Deswanto, Isaac A. Iswara, Raja AFW. Iswara, Raja AW. Ivan Rizal Sini, Ivan Rizal Joedo Prihartono Levina S. Pakasi Lisnawati Rachmadi Meilinda, Astrid Mewahyu Dewi, Mewahyu Michelle Eva Mohammad Sulchan Namira Kesuma Jelita Nur Rasyid Nur Rasyid P Pryambodho, P Pakpahan, Karimul Abdi Pande Made Wisnu Tirtayasa Prahara Yuri Prasetyo, Dimas Tri Pudji Sari Puji Sari Purba, Herdiman Bernard Puspita E. Wuyung Putra, Donny E. Raharja, Putu Angga Risky Rahmad Mulyadi Retno A. Werdhani Retno Asti Werdhani Savira, Missy Savitri, Ary Indriana Selanno, Fradelino Esau Siti Setiati Siti T.K. Gardian Surya Putra, Abdul Hafiidh Suryoadji, Kemal Akbar Sutisna Himawan Tommie Prasetyo Tommie Prasetyo, Tommie Tri Indah Winarni Tri Prasetyo, Dimas Uiterwaal, Cuno Vinny Verdini Widi Atmoko Widyasmara, Her Bayu Wistara, Sevita Sathya Yopie Tjandradiguna Satyawan Yuli Budiningsih Zulfikar Ali Zulfikar Ali, Zulfikar