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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Pharmascience REINWARDTIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry Action Research Literate (ARL) Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Global Forest Journal Policy Brief Pertanian, Kelautan, dan Biosains Tropika Reinwardtia Indonesian Journal of Forestry Research
Fifi Gus Dwiyanti
Department Of Silviculture, Faculty Of Forestry And Environment, IPB University, IPB Darmaga Campus, Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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POPULATION GENETICS OF THE CRITICALLY ENDANGERED SPECIES Dipterocarpus littoralis Blume (Dipterocarpaceae) ENDEMIC ON NUSAKAMBANGAN ISLAND, INDONESIA Dwiyanti, Fifi Gus; Harada, Ko; Siregar, Iskandar Zulkarnaen; Kamiya, Koichi
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17150.467 KB) | DOI: 10.11598/btb.2014.21.1.304

Abstract

Background Dipterocarpus littoralis Blume is a critically endangered dipterocarp species found only on Nusakambangan Island, Central Java, Indonesia. Patterns of genetic diversity and population genetic structure of adults and saplings in two extant populations (Kali Jati and Solok Besek) were estimated using ten microsatellite markers. Results A total of 39 alleles was found, with two and four alleles being unique in adult and sapling populations, respectively. Allelic richness and heterozygosity was similar between adult (Ar = 3.00; He = 0.423) and sapling (Ar = 3.25; He = 0.441) populations.Inbreeding coefficientsin saplingswere positive in both populations and statistically significant in Kali Jati, while those in adult populations were not significantly different from zero, indicating excessive inbreeding and selfing in the sapling populations. Genetic differentiation of the sapling populations (FST = 0.036) was slightly lower than in the adult populations (0.050), but only significantly so for saplings. Conclusions This study revealed that D. littoralis has low genetic diversity in both adults and saplings. Similarly low values in allele richness and heterozygosity suggest that reductions of population size have been ongoing for long periods in this species. Significant genetic differentiation between sapling populations but not adult populations indicates that recent fragmentation is further accelerating the isolation process.
Optimizing DNA Extraction Methods from Leaf and Wood Tissues to Support Dipterocarp Conservation and Sustainable Forest Management Arrofaha, Nawwall; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Adinugroho, Wahyu Catur; Siregar, Iskandar Zulkarnaen; Kamal, Irsyad; Syaputra, Dhika; Salamah, Andi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 1 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.1.13

Abstract

Dipterocarpaceae are economically important, contributing over 85% of Indonesia's timber exports. However, this crucial resource is increasingly threatened by illegal logging, habitat destruction, and the illegal timber trade, which jeopardize dipterocarp population. Furthermore, conservation efforts utilizing genetic and forensic techniques often encounter substantial challenges due to the complexities in DNA extraction protocol. To address this, the study aimed to enhance the efficiency of DNA extraction methodologies by comparing two methods: the modified cetyltrimethylammonium bromide (CTAB) and the Genomic DNA Mini Kit (Plant) from Geneaid Biotech Ltd. The research focused on leaf and wood samples from two species, spesifically Rubroshorea leprosula (Miq.) P.S.Ashton & J.Heck and Shorea laevis Ridl. For each of these species, five leaf and five wood samples were extracted using both methods. The quality of the DNA extraction was evaluated using electrophoresis and quantified with a Qubit fluorometer. Higher DNA concentrations were obtained with the modified CTAB method compared to the GeneAid kit for both R. leprosula and S. laevis, particularly in leaf tissue. The GeneAid kit consistently exhibited low DNA yield efficiency compared to the modified CTAB method for both species. Additionally, PCR amplification of both leaf and wood samples confirmed that the extracted DNA was suitable formolecular analyses. These findings not only contributed to laboratory applications but also served as practical tools for species identification and genetic conservation for sustainable forest management and law enforcement.
Analisis Bahaya Longsor Berdasarkan Sistem Informasi Geografis di Kabupaten Sumedang Rakhmad Fadillah; Boedi Tjahjono; Fifi Gus Dwiyanti
Jurnal Penelitian Pendidikan IPA Vol 10 No SpecialIssue (2024): Science Education, Ecotourism, Health Science
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10iSpecialIssue.8354

Abstract

Sumedang Regency has a hilly landscape, making it one of the 13 cities/regencies in West Java Province that are prone to landslides. A total of 80 landslide incidents were recorded from 2019 to 2023. These landslides resulted in 45 fatalities, 53 injuries, and damage to 317 infrastructure units. This situation indicates the importance of conducting an analysis of landslide hazard distribution. The landslide hazard distribution analysis is carried out using a weighting and scoring method on the parameters used, which include: slope gradient, rainfall, actual land cover, landform, lithology, and soil type. Based on these parameters, four landslide hazard classes were identified in Sumedang Regency: low, medium, high, and very high hazard classes. Proportions of these hazard are as follows: high hazard class (42.24%), medium hazard class (40.38%), low hazard class (13.90%), and very high hazard class (3.49%). The low hazard class is mainly found in the northern part of Sumedang Regency, the medium hazard class is widespread in sloping areas, and the high to very high hazard classes are primarily found in the Tampomas mountains and areas with hilly landforms. Slope gradient and rainfall are the factors that most influence landslide hazards, making it necessary to design appropriate mitigation.
Metabolite Profiling of The Antioxidant Properties of The Aquatic Plant Cyperus involucratus Employing UHPLC-HRMS Windyaswari, Ari Sri; Faramayuda, Fahrauk; Billa, Salsa Rizqyana; Miarsih, Maria Tri; Ayu, Inna Puspa; Pratiwi, Niken Tunjung Murti; Krisanti, Majariana; Dwiyanti, Fifi Gus
Journal of Pharmascience Vol 13, No 1 (2026): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v13i1.24618

Abstract

The genus Cyperus is widely recognized as decorative flora. Empirically, Cyperus plants have been utilized to treat wounds, inflammation, and digestive disorders. While the genus is reported to exhibit antioxidant properties, scientific data regarding Cyperus involucratus remains limited. This research aimed to identify antioxidant compounds in C. involucratus using Ultra-High-Performance Liquid Chromatography-High Resolution Mass Spectrometry (UHPLC-HRMS). The C. involucratus methanolic extract was obtained through maceration using a 70% methanol solvent. Separation was performed using a UHPLC gradient system with a flow rate of 0.3 mL/min , while compound identification was conducted using a Quadrupole-Orbitrap MS detector in positive mode. The radical scavenging activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analysis identified 175 metabolites within the methanolic extract. Based on mzCloud library matching with confidence scores of 97.4–100%, 12 primary bioactive compounds were identified: oleamide, α -linolenic acid, kaempferol, scutellarin, hexadecanamide, 1-linoleoyl glycerol, 1-stearoy-rac-lglycerol, adenine, adenosine, L-tyrosine, stearamide and nootkatone. The extract possessed a moderate radical scavenger with an IC50 value of 57.94 μg/mL. These findings provide a scientific foundation for further investigation of C. involucratus as a potential natural antioxidant, although further in vivo studies are required to validate its broader pharmacological outcomes.
Co-Authors Adinugroho, Wahyu Catur Adzkia, Ulfa Alfi Hudatul Karomah Alfian Helmi Amigunani, Zahra Wajdini Amperanoto, Agus Andi Salamah Ari Sri Windyaswari, Ari Sri Arida Susilowati Iswanto Arief Sabdo Yuwono Arrofaha, Nawwall Aulia Andi Mustika Ayu, Inna Puspa Ayyasy, Yahya Billa, Salsa Rizqyana Boedi Tjahjono Budi Purwanto Cut Rizlani Kholibrina Deden Derajat Matra Dien Atin Boritnaban Dodik Ridho Nurrochmat Dwi Utami , Anisa Fahrauk Faramayuda, Fahrauk Febrita, Joana Handian Purwawangsa Henti Hendalastuti Rachmat Henti Rosdayanti, Henti Hudaya, Aang Husin Alatas IBNUL QAYIM Ida Mallia Ginting IPB, BPKB Irsyad Kamal Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Khairunnisa, Zayyaan Nabiila Khoirunisa, Hana Ko Harada Ko Harada Koichi Kamiya Koichi Kamiya Kustiyarini, Nur Fadila KUSUMADEWI SRI YULITA Laswi Irmayanti, Laswi Majariana Krisanti Majiidu, Muhammad Matra, Deden Derajat Meti Ekayani Miarsih, Maria Tri Mohamad Rafi Muhammad Majiidu Muhammad Majiidu Muharam, Karima Fauziah Mutya Kana Purba Niken Tunjung Murti Pratiwi Nyoto Santoso Putra, Heriansyah Rachmat, Henti Hendalastuti Rahadian Pratama Rahman, Mohamad Miftah Rai Sita Rakhmad Fadillah Rifnadhi, Bayu RIKA RAFFIUDIN Rina Mardiana Rukmana, Adi Sari, Windi Mayang Septaningsih, Dewi Anggraini Siska Nurfajri Slamet, Alim S Susila, Susila Syaputra, Dhika Ulfah Juniarti Siregar Utami, Anisa Dwi Utami, Annisa Dwi Wati, Ridha WINDRA PRIAWANDIPUTRA, WINDRA Zaenal Abidin