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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) REINWARDTIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry Action Research Literate (ARL) Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Global Forest Journal Policy Brief Pertanian, Kelautan, dan Biosains Tropika Reinwardtia Indonesian Journal of Forestry Research
Fifi Gus Dwiyanti
Department Of Silviculture, Faculty Of Forestry And Environment, IPB University, IPB Darmaga Campus, Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary

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Optimizing DNA Extraction Methods from Leaf and Wood Tissues to Support Dipterocarp Conservation and Sustainable Forest Management Arrofaha, Nawwall; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Adinugroho, Wahyu Catur; Siregar, Iskandar Zulkarnaen; Kamal, Irsyad; Syaputra, Dhika; Salamah, Andi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 1 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.1.13

Abstract

Dipterocarpaceae are economically important, contributing over 85% of Indonesia's timber exports. However, this crucial resource is increasingly threatened by illegal logging, habitat destruction, and the illegal timber trade, which jeopardize dipterocarp population. Furthermore, conservation efforts utilizing genetic and forensic techniques often encounter substantial challenges due to the complexities in DNA extraction protocol. To address this, the study aimed to enhance the efficiency of DNA extraction methodologies by comparing two methods: the modified cetyltrimethylammonium bromide (CTAB) and the Genomic DNA Mini Kit (Plant) from Geneaid Biotech Ltd. The research focused on leaf and wood samples from two species, spesifically Rubroshorea leprosula (Miq.) P.S.Ashton & J.Heck and Shorea laevis Ridl. For each of these species, five leaf and five wood samples were extracted using both methods. The quality of the DNA extraction was evaluated using electrophoresis and quantified with a Qubit fluorometer. Higher DNA concentrations were obtained with the modified CTAB method compared to the GeneAid kit for both R. leprosula and S. laevis, particularly in leaf tissue. The GeneAid kit consistently exhibited low DNA yield efficiency compared to the modified CTAB method for both species. Additionally, PCR amplification of both leaf and wood samples confirmed that the extracted DNA was suitable formolecular analyses. These findings not only contributed to laboratory applications but also served as practical tools for species identification and genetic conservation for sustainable forest management and law enforcement.
Analisis Bahaya Longsor Berdasarkan Sistem Informasi Geografis di Kabupaten Sumedang Rakhmad Fadillah; Boedi Tjahjono; Fifi Gus Dwiyanti
Jurnal Penelitian Pendidikan IPA Vol 10 No SpecialIssue (2024): Science Education, Ecotourism, Health Science
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10iSpecialIssue.8354

Abstract

Sumedang Regency has a hilly landscape, making it one of the 13 cities/regencies in West Java Province that are prone to landslides. A total of 80 landslide incidents were recorded from 2019 to 2023. These landslides resulted in 45 fatalities, 53 injuries, and damage to 317 infrastructure units. This situation indicates the importance of conducting an analysis of landslide hazard distribution. The landslide hazard distribution analysis is carried out using a weighting and scoring method on the parameters used, which include: slope gradient, rainfall, actual land cover, landform, lithology, and soil type. Based on these parameters, four landslide hazard classes were identified in Sumedang Regency: low, medium, high, and very high hazard classes. Proportions of these hazard are as follows: high hazard class (42.24%), medium hazard class (40.38%), low hazard class (13.90%), and very high hazard class (3.49%). The low hazard class is mainly found in the northern part of Sumedang Regency, the medium hazard class is widespread in sloping areas, and the high to very high hazard classes are primarily found in the Tampomas mountains and areas with hilly landforms. Slope gradient and rainfall are the factors that most influence landslide hazards, making it necessary to design appropriate mitigation.
Exploring Students' Perception on Sustainable University: Empirical Findings in IPB University, Indonesia Utami, Anisa Dwi; Qayim, Ibnul; Putra, Heriansyah; Mardiana, Rina; Dwiyanti, Fifi Gus; Khoirunisa, Hana; Khairunnisa, Zayyaan Nabiila
Journal of Sustainability Perspectives Vol 5, No 1 (2025)
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jsp.2025.26215

Abstract

Universities play a key role in driving positive change by equipping students to address environmental and social challenges. This study examines how students at IPB University perceive various sustainability initiatives implemented on campus. Based on a survey of 1,545 undergraduate students, the research explores their knowledge, awareness, and interest in these efforts. The results show that while most students are familiar with the general concept (87.6%), fewer have knowledge of specific programs, institutional rankings, or the university’s Campus Sustainability Office. Nevertheless, nearly all respondents consider these efforts important (98.7%) and support active student involvement (97.2%). Many also express a desire to see these topics integrated into academic courses (84.9%).These findings highlight the need for improved internal communication and curriculum development to enhance student participation. The study offers both practical and academic insights for improving sustainability strategies in higher education institutions. Future research should include multiple universities and consider mixed-method approaches to better understand the factors influencing student engagement
Co-Authors Adinugroho, Wahyu Catur Adzkia, Ulfa Alfi Hudatul Karomah Alfian Helmi Amigunani, Zahra Wajdini Amperanoto, Agus Andi Salamah Arida Susilowati Iswanto Arief Sabdo Yuwono Arrofaha, Nawwall Aulia Andi Mustika Ayyasy, Yahya Boedi Tjahjono Budi Purwanto Cut Rizlani Kholibrina Deden Derajat Matra Dien Atin Boritnaban Dodik Ridho Nurrochmat Dwi Utami , Anisa Febrita, Joana Handian Purwawangsa Henti Hendalastuti Rachmat Henti Rosdayanti, Henti Hudaya, Aang Husin Alatas IBNUL QAYIM Ida Mallia Ginting IPB, BPKB Irsyad Kamal Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Khairunnisa, Zayyaan Nabiila Khoirunisa, Hana Ko Harada Ko Harada Koichi Kamiya Koichi Kamiya Kustiyarini, Nur Fadila KUSUMADEWI SRI YULITA Laswi Irmayanti, Laswi Majiidu, Muhammad Matra, Deden Derajat Meti Ekayani Mohamad Rafi Muhammad Majiidu Muhammad Majiidu Muharam, Karima Fauziah Mutya Kana Purba Nyoto Santoso Putra, Heriansyah Rachmat, Henti Hendalastuti Rahadian Pratama Rahman, Mohamad Miftah Rai Sita Rakhmad Fadillah Rifnadhi, Bayu RIKA RAFFIUDIN Rina Mardiana Rukmana, Adi Sari, Windi Mayang Septaningsih, Dewi Anggraini Siska Nurfajri Slamet, Alim S Susila, Susila Syaputra, Dhika Ulfah Juniarti Siregar Utami, Anisa Dwi Utami, Annisa Dwi Wati, Ridha WINDRA PRIAWANDIPUTRA, WINDRA Yosie Syadza Kusuma Zaenal Abidin