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Yusianti Silviani
Diploma III Program In Medical Laboratory Technology, National Health Science Institute (STIKES Nasional)l
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Other

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Journal : Jurnal Biologi Tropis

 1 
Differences in The Incidence of Urinary Tract Infection (UTI) in Women Using Herbal and Non-Herbal Sanitary Napkins Yanti, Rahma; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8873

Abstract

Urinary Tract Infection (UTI) is a common health issue among women, including those caused by the use of menstrual pads. This study aims to investigate the difference in the incidence of UTI between women using herbal and non-herbal pads at Prodia Clinical Laboratory in Palembang. This research employed an analytical observational design with 31 female employees as subjects. Sampling was conducted using a questionnaire and complete urine tests, including dipstick and microscopic examination. The results showed that 10.5% of non-herbal pad users were suspected of having UTI, while no herbal pad users were suspected of having UTI. Based on the Mann Whitney U statistical test, no significant differences were found between the two groups (p > 0.05). This study concluded that there is no significant difference in the incidence of UTI between users of herbal and non-herbal pads. Further research with a larger sample size is recommended to confirm these findings.
Identification of Neisseria gonorrhoeae in Commercial Sex Worker Clients with and without Condom Use Maulandi, Andi; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9142

Abstract

This study investigates the prevalence of Neisseria gonorrhoeae among commercial sex workers and their clients in Pulau Baai, Bengkulu, with a focus on comparing infection rates between individuals who use condoms and those who do not. The primary objective is to assess the impact of condom usage on the prevalence of gonorrhea in this high-risk population. A cross-sectional observational design was employed, involving 40 male participants who had engaged with commercial sex workers in the Pulau Baai area. The participants were divided into two groups based on their condom use. Urethral secretion samples were collected and analyzed via Gram staining for Neisseria gonorrhoeae detection. The study found no cases of Neisseria gonorrhoeae in any of the samples, with all 36 condom users and 4 non-users testing negative. These results suggest no gonorrhea infections in the studied population, regardless of condom use. However, due to the limitations of the Gram staining method, further research using more sensitive diagnostic techniques and a larger sample size is recommended to verify these findings.
Identification of Neisseria Gonorrhoeae in Female Sexual Workers (FSW) Localized on Baai Island, Bengkulu City Lenzu, Henrek Miko; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9152

Abstract

Sexually Transmitted Infections (STIs) pose a significant global health challenge, particularly among high-risk populations such as female sex workers (FSWs). Neisseria gonorrhoeae, a causative agent of gonorrhea, is a major concern due to its prevalence and transmission via sexual contact. This study aims to identify the presence of Neisseria gonorrhoeae in female sex workers (FSWs) at the Pulau Baai locality, Bengkulu City, and examine the correlation between infection rates and factors such as duration of work and frequency of client visits. A descriptive observational study with a cross-sectional design was conducted, involving 31 FSWs who participated in the study after providing informed consent. Samples were collected through vaginal swabs and examined using Gram staining to identify Neisseria gonorrhoeae. The findings revealed that 3.2% (1 out of 31) of the participants tested positive for Neisseria gonorrhoeae. Statistical analysis showed that the risk of infection increased with the duration of work, as well as the frequency of client visits. The study concludes that while the overall infection rate is low, the prevalence of Neisseria gonorrhoeae remains a concern in this high-risk group. Regular screenings and preventive measures, including consistent condom use, are recommended to reduce transmission among FSWs.
Difference Results Bacteria Numbers with Variations Temperature and Check Time in The Elderly Urine Suspect Urinary Tract Infections (UTI) Septiani, Risma Dyah; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6723

Abstract

Delays in examination have the potential to result in differences in the results of the number of bacteria in the examined specimens plus storage of specimens at non-optimal temperatures. The difference in the number of bacteria can be known by examining the Total Plate Count (TPC) in urine. The purpose of this study was to determine the differences in the results of germ numbers in terms of variations in examination delays and urine storage temperatures of elderly suspects with urinary tract infections (UTI).  The type of research conducted was experimental analytic with the subject of 4 elderly urine in Kratonan Village, Surakarta with criteria sampling technique. Research data were obtained through questionnaires and examination of total plate numbers with mayo technique examination, where the results were multiplied by a conversion factor of 102 CFU/ml.  The results were analyzed by SPSS statistical test using Two Way ANOVA which obtained a significant value of 0.915 (p>0.05). Then continued with SPSS data analysis using Tukey Pos Hoc aims to see the real difference of each variation. There is no difference in the germ count results with temperature variations and time delays in the urine of elderly suspected urinary tract infection (UTI) patients.
Microscopic Examination of Acid-Resistant Bacils in Closed Contacts of Patients With Tuberculosis in The Working Area Of The UPTD Puskesmas Cilegon Rahayu, Ayu Puji; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.6980

Abstract

Tuberculosis is a contagious disease caused by Mycobacterium tuberculosis. The transmission of pulmonary TB occurs by the emission of droplets who are positive for the disease. Attempts were undertaken to examine interactions between cadres and the community. The patient's close contacts underwent microscopic inspection utilising the Ziehl Neelsen staining procedure. Between 2023 and October, a total of 360 cases of tuberculosis will be investigated using the ZN painting method. Among the close contacts of the 72 patients, 20% (6 individuals) will be identified as having tuberculosis. This study aimed to assess the outcomes of BTA microscopic examination conducted on close household contacts within the operating region of UPTD Puskesmas Cilegon in 2024. The research method employed a descriptive observational approach with a cross-sectional design. The study was carried out from 1 February to 9 March 2024 at the UPTD Puskesmas Cilegon. The sample technique employed is purposive sampling. The study findings revealed that out of the total number of respondents, 32 individuals reported having direct contact with individuals affected by tuberculosis. Among these respondents, 13 (40.6%) were male and 19 (59.4%) were female. Out of the 32 individuals who had close contact, one respondent (3.3%) tested positive for BTA when microscopic inspection was conducted. The proximity to the afflicted individual directly correlates with the heightened likelihood of transmission. Proximity between those who are infected and their close contacts can lead to the transmission of the infection, even when sharing the same sleeping space.
Antibacterial Efectiveness Test of Basil Leaf Ethanol Extract (Ocinum sanctum L) Against Streptococcus pneumoniae ATCC 6301. Khasanah, Barhoroh Nurul; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8601

Abstract

Basil leaves, which include flavonoids, alkaloids, saponins, tannins, and phenols, are one of the herbal plants that may be used as an antibiotic. We set out to see whether our hypothesis that an ethanol extract of basil leaves inhibited the development of Streptococcus pneumoniae ATCC 6301 bacteria would hold water. Methods from descriptive experimental research are employed in this work. As part of its Bacteriology Laboratory, the National College of Health Sciences carried out the investigation. Quantitative sampling is the method employed. Foliage picked fresh from a basil field in Klaten's Jogonalan neighborhood. Powdered basil leaves were isolated by maceration with 96% ethanol. A series of concentrations of 20%, 40%, 60%, 80%, and 100% DMSO were subsequently applied to the extract. A Kirby-Bauer assay for determining antimicrobial efficacy. Findings showed that an extract from basil leaves was antibacterial against the strain of Streptococcus pneumoniae ATCC 6301. On average, the inhibition zone diameter for basil leaf extract at 0%,40%,60%,80%, and 100% was 10.4 mm, 10.8 mm, 11.5 mm, 11.9 mm, and 13.0 mm, respectively. A 13.0 mm bland zone was generated by the maximum effective concentration of 100% in inhibiting Streptococcus pneumoniae ATCC 6301. No matter the dose, the antibacterial effects of basil leaf extract (Ocimum x africanum Lour) were able to suppress the growth of Streptococcus pneumoniae ATCC 6301. able to block 13.0 mm. C.
Co-Authors Adisty, Candrika Andriyani - Puspitaningrum Andriyani Puspitaningrum Anjarani, Ajeng Viona Putri Anjaswari, Vivi Capawati Anjela Bella Dora Natalia Ardy Prian Nirwana Ardy Prian Nirwana, Ardy Prian Astriana, Sevy Astuti Setiyani Bambang Hadi Sugito Binuko Amarseto Darjati Dedi Kurniawan Demes Nurmayanti Didik Wahyudi Didik Wahyudi Dwi Iswanto, Endro Endarini, Lully Hani Evi Yunita N Hadi Suryono Halwatiah, Halwatiah Handayani, Susanti Herlinda Darmastuti Indah Lestari, Indah Indito, Adelio Szaky Putra Indriati, Elli Ira Puspita Sari Isfentiani, Dina Juliana Christyaningsih Kartika Pertiwi Khambali, Khambali Khasanah, Barhoroh Nurul Kiaonarni OW Kusumaningrum, Septiana Lembunai Tat Alberta, Lembunai Tat Lenzu, Henrek Miko Leonardo Bagus Utomo Maulandi, Andi Mulatningtyas, Zevanya Mutianingsih, Nur Mutiarawati, Diah Titik N. S. Widodo Niassinta, Julita Novita Sari, Dyah Ayu Nur Hatijah Nurhidayat Nurhidayat Octaviana Ikka Sandra Susanti Pembayun, Raflesia Rasmiati Putri Pengge, Nuning Marina Pramuditha, Zevanya Mulatningtyas PuriMahaliya, Karisma Puspitadewi, Teresia Retna Puspitasari, Afita Indah Putri, Alifa Purwahari Putu Salsabila Rida Okta Rahayu, Ayu Puji Rahayuningsih, Christ Kartika Rahmawati, Afrilia Retno Sasongkowati Sari, Aprilia Saroh, Dewi Septiani, Erni Dwi Septiani, Risma Dyah Septiyan, Erni Dwi Siagian, Hotmaida Siti Alfiah Suciati Sudarisman Sugiyarto Sugiyarto Suliati Sumasto, Hery Sungkar, Rizky Fadzillah Susanti Handayani Syafa, Alya Nur Tantias, Farmasiriana Deli Tri Harningsih, Tri Wahono Putri, Yordha Maharani Wandan Wisdyafanny, Marcelina Wimpy, W Yanti, Rahma Yulianti, Adhiesti Nur Yulianti, Widya Ratri Intan Yuniarta, Abdullah Burhan Zuhria Nurullita