Article Per Year (5 Year)
p-Index From 2021 - 2026
1.633
P-Index
This Author published in this journals
All Journal Jurnal Mirai Management RABIT: Jurnal Teknologi dan Sistem Informasi Univrab Ekonis : Jurnal Ekonomi dan Bisnis Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Gemilang: Jurnal Manajemen dan Akuntansi Aspirasi : Publikasi Hasil Pengabdian dan Kegiatan Masyarakat Jurnal Ilmiah Manajemen Dan Kewirausahaan
Aryati Aryati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Health Professions Medicine & Pharmacology Neuroscience Public Health

Published : 37 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 37 Documents
Search

 1   2   3   4 
KAITAN IgE SPESIFIK METODE IMUNOBLOT TERHADAP ELISA PADA RINITIS ALERGI (Association Between Specific IgE Immunoblot Method with ELISA on Allergic Rhinitis) Aryati Aryati; Dwi Retno Pawarti; Izzuki Muhashonah; Janti Tri Habsari
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1284

Abstract

Allergic rhinitis is an allergic disease that is most often found beside bronchial asthma and eczema with the prevalence of is about33.3%, 9.8% and 11.2% respectively. The main examinations of allergic rhinitis are Skin Prick Test (SPT) and specific IgE, becausethe sensitivity and specificity of specific IgE examination depend on the examination method. To know the diagnostic value of specificIgE immunoblot examination by determination and were compared with ELISA in patients with allergic rhinitis. The cross-sectionaldesign of the study is con-ducted on patients at the Outpatient Clinic Department of ENT-Head and Neck from May until October 2014.Patients were grouped as diagnosis of allergic rhinitis and non-allergic non-infectious rhinitis based on clinical signs and symptoms,physical examina-tion, positive in SPT examination with or without an increase in total serum IgE and/or blood eosinophils. SpecificIgE immunoblot was conducted by using Foresight®, Acon Laboratories and the ELISA method using Allercoat™. The sensitivity andspecificity of inhalant allergen -specific IgE immunoblot Foresight® method was 73.9% and 42.9%, respectively. The sensitivity andspecificity of inhalant allergen -specific IgE ELISA method was 67.4% and 57.1%, respectively. The results of these two methods havea correlation coefficient 0.531 with p=0.000. The sensitivity and specificity of ingestan allergen specific IgE immunoblot Foresight®method was 41.3% and 85.7%, respectively. The sensitivity and specificity of ingestan allergen specific IgE ELISA method was 17.4 and78.6%, res-pectively. Results of these two methods have a correlation coefficient 0.375 with p=0.003. Based on this study of specificIgE immunoblot and ELISA methods, both have diagnostic sensitivity and specificity, which are almost the same. The sensitivity ofimmunoblot method inhalant allergens are superior to ELISA. The Immunoblot method ingestan allergen specificity is superior toELISA.
NILAI DIAGNOSTIK KASET IMUNOKROMATOGRAFI SEBAGAI ALAT PENUNJANG DIAGNOSIS DEMAM BERDARAH DENGUE PADA PENDERITA DEWASA Kusuma Pindayani; Aryati Aryati; Y. Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.887

Abstract

A rapid immunochromatographic test that incorporates blue particle conjugated with recombinant dengue envelope protein,antihuman IgM monoclonal antibodies and antihuman IgG monoclonal antibodies (Virotec Dengue IgG/IgM XP; Indec Diagnostics,Indonesia using technology of USA) was evaluated by using IgG and IgM captured ELISA (Dengue Duo ELISA, Panbio) which has a99% sensitivity and a 92% specificity as the “gold standard”. This assay, cassette in shape, is performed in 15–30 minutes and detectsboth immunoglobulin M (IgM) and IgG in a capture format. There were 57 paired sera of dengue haemmorhagic fever (according toWHO 1997 criterias) and 72 sera of non dengue haemmorhagic fever patients (typhoid fever, UTI, malaria, chikungunya, hepatitis andpneumonia). For the diagnosis of dengue virus infection, the rapid test showed a sensitivity of 98%, positive predictive value of 88%,negative predictive value of 98% and specificity of 99%. The rapid test is a useful aid in rapid diagnosis and differentiation betweenprimary and secondary dengue virus infection.
NILAI DIAGNOSTIK ANTI DENGUE IgA DAN NS1, SERTA IgM/IgG DI INFEKSI VIRUS DENGUE (The Diagnostic Value of Anti Dengue IgA and Anti Dengue IgM/IgG in Dengue Virus Infection) Resna Resna; Aryati Aryati; Puspa Wardhani; Erwin Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1264

Abstract

The clinical manifestations of dengue virus infection are varied and thus a specific diagnostic examination is required. Usually antidengueIgM is often used, but the presence in the circulation is 3−8 months long. NS1 is sensitive in the detection of primary infection,whereas IgG is more better used in secondary infection. The examination of anti-dengue IgA as a new marker is estimated to be ableto detect the acute primary and secondary infection, however the diagnostic value of anti-dengue IgA is not much well known for theIndonesian population. This study was done at the Tropical Infectious Disease Ward of Dr. Soetomo Hospital, Surabaya during February– April 2013. The samples consisted of 37 sera from patients infected by dengue virus and 37 sera from those non one (dengue virusinfection patients). The NS1 serum, anti-dengue IgM and anti dengue IgG were examined by ELISA and anti-dengue IgA was examined byan indirect immunochromatography method using Assure@ Dengue IgA Rapid Test (MP Biomedicals Asia Pacific Pte Ltd). The diagnosticvalue was analyzed by 2x2 table with a confidence interval of (CI) 95%. The used gold standards were from the 1997th WHO criteriaand one of the positive dengue serological tests by ELISA (NS1/anti dengue IgM/anti dengue IgG). AUC and anti-dengue IgA cut-off weredetermined by ROC curve. The Diagnostic value of anti-dengue IgA showed a sensitivity and specificity of 83.8% (67.3 to 93.2) and 81.1%(64.3 to 91.4). A positive predictive value of 81.6% (65.1 to 91.7) and a negative predictive value of 83.3% (66.5 to 93.0) was found. Thepositive likelihood ratio was 4.4 times (2.2 to 8.8) and negative likelihood ratio of only 0.2 times (0.09 to 0.42). The best cut off valueof 0.2 was shown by the area under the curve of 83.5%. Based on this study, the diagnostic value of anti-dengue IgA had a good validityfor the diagnosis of dengue virus infection.
RELATIONSHIP BETWEEN D-DIMER LEVEL AND CLINICAL SEVERITY OF SEPSIS Yessy Puspitasari; Aryati Aryati; Arifoel Hajat; Bambang Pujo Semedi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1196

Abstract

D-dimer merupakan tolok ukur laboratorium yang menunjukkan derajat keparahan pada sepsis. Selama tahapan sepsis terjadiaktivasi prokoagulan yang tidak diimbangi aktivitas antikoagulan (depresi protein C dan meningkatnya pelepasan Plasminogen activatorinhibitor) sehingga dapat meningkatkan hasilan fibrin polimer. Fibrin polimer yang telah mengalami cross-linked akan difibrinolisis olehplasmin membentuk formasi D-dimer. Tujuan penelitian untuk menganalisis hubungan D-dimer dengan derajat keparahan klinis darisepsis. Metode penelitian bersifat potong lintang observasional. Sampel darah sitrat dari 52 pasien sepsis yang dirawat di IRD, ICU, ROI,Ruang penyakit dalam RSUD. Dr. Soetomo Surabaya, dikumpulkan selama Februari 2016–Juni 2016. Kadar D-dimer diukur denganmetode ELFA (Enzyme Linked Fluorescent Assay). Proses dan tafsiran data menggunakan analisis deskriptif, One sample Kolmogorovsmirnovdan uji Pearson digunakan untuk menganalisis kenasaban. Didapatkan rerata kadar D-dimer 3879,46±2800,29 ng/mL.D-dimer pada non-survivors sepsis menurut skor APACHE II dan SOFA lebih tinggi daripada survivors sepsis. Terdapat kenasabanpositif yang bermakna antara kadar D-dimer dengan skor APACHE II dan skor SOFA r=0,513 dan r=0,580 (p=0,01). Berdasarkantelitian ini dapat disimpulkan D-dimer memiliki kenasaban dengan derajat keparahan klinis dari sepsis, semakin tinggi nilai D-dimermenunjukkan keparahan sepsis.
DETEKSI ANTIBODI MULTIPEL HEPATITIS C DALAM DARAH DONOR (Multiple Antibody Detection of Hepatitis C in Donor Blood) Ranti Permatasari; Aryati Aryati; Budi Arifah
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1278

Abstract

Hepatitis C (HCV) infection could be spread by blood transfusion. Screening of HCV in donor blood could prevent HCV infection to therecipient. HCV antibody test using rapid test of multiple antibody detection by immunochromatography method is an easy and rapid testthat could detect four HCV antibodies separately. The aim of this study was to evaluate the diagnostic value of antibody HCV using multipleantibody detection rapid test in diagnosing HCV infection. This was an analytical observational study with a cross sectional design. Thesamples consisted of 42 donors’ blood serum from the Surabaya Branch of the Indonesian Red Cross which underwent HCV infectiontest using ELISA method. The samples were then tested using PCR HCV RNA as the gold standard and antibody HCV multiple antibodydetection rapid test The diagnostic value of HCV antibody test using multiple antibody detection rapid test by immunochromatographymethod showed a diagnostic sensitivity of 100%, diagnostic specificity of 75%, positive predictive value of 66.7% and negative predictivevalue of 100%, a diagnostic efficiency of 83.3%, with a positive probability ratio of 4 times. The most often positive antibody patternwas four (4) positive antibodies (core protein, NS3, NS4 and NS5). Core protein (CP) and NS3 were the most often positive antibodies.Based on this study result, the HCV antibody test using multiple antibody detection rapid test by immunochromatography method hasa good diagnostic value.
LIMFOSIT T CD4+SEBAGAI PERAMAL PERJALANAN PENYAKIT PASIEN YANG MENGALAMI SEPSIS Lestari Ekowati; Aryati Aryati; Hardiono Hardiono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 3 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i3.418

Abstract

Sepsis is the most common cause of ICU mortality in USA. Mortality of sepsis in developing countries is still very high, about 50- 70% and has became a 80% incidence in septic shock. There was a decrease of CD4+ T lymphocyte count in patients with sepsis caused by apoptosis indicating septic patients suffered from immune functional impairment. CD4+ T lymphocyte count can reflect the severity of sepsis and predict the prognosis of the patients with sepsis effectively. Eighty eight (88) patients who met sepsis criteria were studied. The researchers collected clinical variables of all patients within 24 hours diagnosis of sepsis, and calculated APACHE II score. At the same time, blood sample were taken to measure the CD4+ T lymphocyte count. The data were analyzed using independent Student-T-test and ROC curve was used for prognosis. There is a significant difference in CD4+ T lymphocyte count between non survival and survival group (non survival group 203±178 cells/μL, survival group 442±303 cells/μL, p<0.001), and the percentage of CD4+ T lymphocyte (non survival group 25.05±11.55%, survival group 34.38±9.15%, p<0.001). There is an under ROC curve for CD4+ T lymphocyte count was 0.81, and for the percentage of CD4+ T lymphocyte was 0.748. Cut off value for CD4+ T lymphocyte count was 204 cells/μL, and the percentage of CD4+ T lymphocytes was 25.23%. Based on this study, the CD4+ T lymphocyte count can be used as a predictor of prognosis in sepsis patients.
DIAGNOSTIC VALUE OF FASTSURE TB DNA RAPID TEST FOR DIAGNOSIS OF PULMONARY TUBERCULOSIS Diyan Wahyu Kurniasari; Jusak Nugraha; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1203

Abstract

Diagnosis tuberkulosis (TB) di Indonesia menurut Kemenkes RI 2014 masih mengandalkan pemeriksaan mikroskopis Basil TahanAsam (BTA) dari hapusan dahak namun memiliki kepekaan dan kekhasan diagnostik yang rendah. Kepekaan diagnostik kulturMycobacterium tuberculosis (M.tuberculosis) pada media Lowenstein Jensen (LJ) lebih tinggi daripada mikroskopis BTA namun hasilnyamemerlukan waktu 6-8 minggu. Uji cepat Fastsure TB DNA menggunakan metode Cross Priming Amplification (CPA) merupakanuji deteksi kualitatif DNA M.tuberculosis yang diamplifikasi pada satu suhu tetap dan menggunakan nucleic acid lateral flow stripdalam perangkat plastik tertutup. Penelitian ini dilakukan untuk menilai nilai diagnostik uji cepat Fastsure TB DNA untuk diagnosistuberkulosis paru. Metode penelitian secara observasional potong lintang selama Juni sampai November 2015. Total 58 spesimen dahakdari 33 orang terduga tuberkulosis paru dan 25 orang non-tuberkulosis dari RS Paru Karang Tembok Surabaya. Setiap spesimendilakukan pemeriksaan mikroskopis BTA, kultur M.tuberculosis pada media LJ sebagai standar baku emas dan ekstraksi DNA danamplifikasi pada uji cepat Fastsure TB DNA. DNA terekstraksi dimasukkan ke dalam tabung kemudian ke cartridge yang tersedia dalamperangkat. Hasil diamati dalam waktu 30 menit berupa munculnya garis uji dan pembanding pada pita. Berdasarkan penelitian ini,diperoleh kepekaan dan kekhasan diagnostik uji cepat Fastsure TB DNA masing-masing sebesar 84,8% dan 92% dengan koefisien kappaadalah 0,757 menunjukkan kesesuaian yang cukup baik. Uji cepat Fastsure TB DNA merupakan pemeriksaan yang cepat, praktis, relatiftidak mahal untuk diagnosis M.tuberculosis dari spesimen klinis khususnya pada BTA negatif.
ANALISIS FILOGENETIK DENGUE DI INDONESIA Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1009

Abstract

Molecular epidemiology is needed to solve the problem for endemic Dengue Hemorrhagic Fever in Indonesia.This research has been carried out consisting of 525 Dengue Hemorrhagic Fever sera, according to the WHO criteria.These sera were collected from 19 cities in Indonesia comprising the islands of Sumatera, Batam, Kalimantan, Sulawesi, Papua, Java,Bali and Lombok from 2003 until 2005. The immune response profile was as follows 57.14% (300/525) secondary infection, 12.57%(66/525) primary infection, 4.20% (22/525) equivocal and 26.09% (137/525) negative. From 192 PCR samples, 100 (52%) serawere positive, consisting of 65% DEN-2, 15% DEN-3, 12% DEN-4 and 8% DEN-1. Homology analysis showed nucleotide differences incapsid region DEN-2 serotypes, while DEN-3 serotypes were relatively consistent. Phylogenetic analysis using envelope (E) gene revealedthat the Cosmopolitan genotype from Gorontalo in 2005, is currently circulating locally, with the potential to cause a severe hemorrhagicdisease. Members of this genotype were closely related to viruses from Malaysia, Singapore, Thailand, Philippines and Australia. Theisolate from Jakarta, 2003 showed DEN-3 with I genotype. This genotype was similar to the isolate from Indonesia 1978, 1985, andalso from Thailand 1992, Philippines 1997, and Fiji 1992. These results showed Cosmopolitan genotype from DEN-2 was similar toSoutheast Asia countries. It was also revealed that genotype-I from DEN-3 showed no change over the years since 1978.
PENYAKIT VIRUS EBOLA Henny Elfira Yanti; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1108

Abstract

Ebola virus disease has known as Ebola hemorrhagic fever (EHF) is an acute viral syndrome characterized by fever and bleeding witha high mortality rate in humans and non human (primates). The current outbreak inWestern Africa is the largest ebola outbreak since theebola virus was first discovered in 1976. The first EHF case that reemerged back in Africa occurred in March 2014 and in Desember 29th2014 had been revealed 20,153 cases and 7,883 deaths. The virus is transmitted from wild animals and spread in the human populationthrough human –to -human transmission. Ebola virus infection is characterized by immunosuppression and systemic inflammatoryresponse. Both condition cause the damage of blood vessels, coagulation and disorders of the immune system, leading to multiple organfailure and shock. Until now there are no ebola standards treatment guidelines. However, the life survival increased with early supportivecare such as rehydration and symptomatic treatment.
RAGAM BERBAGAI PERBENIHAN BAKTERI TERKAIT KERENTANANNYA TERHADAP ANEKA JENIS ANTIBIOTIKA Carolina M Viany S; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.960

Abstract

Infection is the major public health problem in Indonesia which could increase its morbidity and mortality. The antibiotics treatmentwhich were given irrationally leads to bacteria susceptibility and worsen the problem. One of the efforts to manage the bacteriasusceptibility the physician has to know the bacterial pattern and its characteristic to resist various antibiotics. The information maycontribute as a reference to give antibiotic therapy in a rational manner. To know the bacterial susceptibility pattern against variousantibiotics a study was carried out using specimens derived from several hospitals which referred them to a private laboratory inSurabaya. The specimens consisted of blood, urine and sputum were referred during September 2007 up to July 2008. The identificationof the bacteria and it‘s susceptibility pattern were carried out by a conventional method and an auto analyzer (Vitek 2 Compact). Theantibiotic susceptibility test was carried out by conventional method using Kirby Bauer modifiied diffusion technique and Vitec 2 Compactusing MIC reference. The results showed that the most common bacteria found from blood was Escherichia coli which was still sensitiveto amoxicillin-clavulanic acid, amikacin, and cefepim. And from the urine was Escherichia coli which were still sensitive to meropenemfollowed by amikacin and gentamycin. Whereas from the sputum was found Streptococcus α haemolyticus which was still sensitive toamoxicillin-clavulanic acid, piperacillin tazobactam, linezolid. The result of the antibiotic susceptibility test is mostly dominated bythe Betalactam group, such as amoxicillin-clavulanic acid and carbapenem group like meropenem. Besides of that, in this study wasalso found multiple drug resistance organisms (MDRO), such as Escherichia coli ESBL, Enterobacter liquefaciens ESBL, Enterobacteragglomerans ESBL, Klebsiella ozaenae ESBL, and Klebsiella pneumoniae ESBL. The susceptibility pattern of the bacteria derived fromblood and sputum is dominated by gram positive cocci. Whereas from urine is dominated by Gram negative rods.
Co-Authors Ade Rochaeni Arifoel Hajat Asnimar Asnimar Ayu Nuryani Ayunda Imaniar Bastiana Bermawi Betty Agustina Tambunan Budi Arifah Carolina M Viany S Devi Rahmadhona Diah Puspitarini Diyan Wahyu Kurniasari Djoko Marsudi Dominicus Husada Dwi Retno Pawarti Erwin Triyono Ety Retno Setyowati Furtasan Ali Yusuf Hardiono Hardiono Henny Elfira Yanti Ismed Wijaya Izzuki Muhashonah Janti Tri Habsari Janti Trihabsari Jeine Stela Akualing Johanis Johanis Juli Soemarsono Jusak Nugraha Kenedi, Kenedi Kusuma Pindayani Lestari Ekowati Lukman Lukman M Fakhriza M Thohirin Ramadhani M. Robi’ul Fuadi M. Y. Probohoesodo M.Y. Probohoesodo Mauli Isra Muahammad Arif Muhammad Vitanata Arfijanto, Muhammad Vitanata Mukhlisul Muzahid Munawaroh - Munawaroh Fitriah Niniek.F. Lantara Pawarti, Dwi Reno Prihatini Prihatini Pudjo Hartono Puspa Wardhani Puspa Wardhani Puspitasari, Yessy Ranti Permatasari Resna Resna Roudhotul Ismaillya Noor Samsudin, Samsudin Semedi, Bambang Pujo Siti Permata Hadi Soedarsono Soedarsono Sri Kartika Sari Syamsul Hidayat Ucy Nadjmiyah Usman Hadi Windu Nafika Y. Probohoesodo