Article Per Year (5 Year)
p-Index From 2020 - 2025
3.018
P-Index
This Author published in this journals
All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Current Biochemistry Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Greensphere: Journal of Environmental Chemistry Prosiding Seminar Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Anto Budiharjo
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Veterinary

Published : 60 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 60 Documents
Search

 1   2   3   4   5 
The Effect of Bacillus altitudinis P-10 Combination Treatments on the Plant Growth and Seed Quality of Corn (Zea mays L) Elfira, Yolanda; Kusmiyati, Florentina; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 22, No 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.22.2.180-187

Abstract

Increasing production of Corn in recent years must be supported with the supply of good quality of seeds. Bacillus altitudinis P -10 is a plant growth promoting rhizobacteria that has biofetilizer and biopesticide activities. The aim of this research was to find out the effect of combination treatments of B. altitudinis P -10 on the growth pf the plant and seed quality of Corn (Zea mays L). The research design used was randomized group design with monofactor pattern of 4 combination treatments with 6 groups. The corn seed used was Lamuru varieties. The treatments consisted of D1 (manure), D2 (manure and B. altitudinis P-10), D3 (manure and chemical fertilizer), and D4 (manure, chemical fertilizer, and B. altitudinis P-10). The parameters observed included the height of the plant, the length of the root, the root dry matter, the crown dry matter, the total dry matter, the emergence time of male flower, the emergence time of female flower, the time of physiological ripening, the length of the cob, the diameter of the cob, the number of seeds per cob, the number of seeds per row per cob, the weight of 100 seeds, the production of the seed, the moisture content, the germination percentage, the purity and the impurity of seed. The results showed that the combination treatments of D4 gave the best parameter for the emergence time of flowers, the time of physiological ripening, the length of the cob, the diameter of the cob, the number of seeds per cob, the number of cobs per row, the weight of 100 seeds, and the production of the seed.
Identifikasi Spesies Mikroalga dari BBPBAP Jepara secara Morfologi dan Molekuler menggunakan 18S rDNA Elke Gildantia; Rejeki Siti Ferniah; Anto Budiharjo; Agung Suprihadi; Muhammad Zainuri; Hermin Pancasakti Kusumaningrum
Buletin Oseanografi Marina Vol 11, No 2 (2022): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v11i2.39703

Abstract

Mikroalga merupakan organisme eukariot bersel satu yang habitatnya berada di perairan. Suatu spesies mikroalga koleksi kultur BBPBAP Jepara berpotensi menghasilkan astaxantin dalam jumlah tinggi. Namun, spesies ini belum di karakterisasi secara molekuler. Penelitian ini bertujuan untuk memperoleh karakter morfologi dan molekuler pada isolat mikroalga dari BBPBAP Jepara menggunakan 18S rDNA guna memastikan spesies untuk pengembangan potensinya. Tahapan penelitian yang dilakukan meliputi kultivasi mikroalga, pengamatan pola pertumbuhan, pengamatan morfologi, isolasi DNA, amplifikasi menggunakan marka 18S rDNA, elektroforesis, analisis data sekuens, dan filogenetik menggunakan aplikasi NJ Plot dan MEGA 7. Hasil karakterisasi secara morfologi sel isolat mikroalga BBPBAP Jepara memperlihatkan bentuk bulat, berwarna hijau dengan ukuran 4,5 μm, tidak memiliki flagela, dan motilitas yang pasif. Hasil karakterisasi molekuler menggunakan 18S rDNA dari isolat mikroalga BBPBAP memperlihatkan kemiripan tertinggi dengan Chlorella sorokiniana sebesar 99%.  Microalgae are single-celled eukariyotic organisms whose habitat is in the waters. A species of microalgae from the Jepara BBPBAP culture collection was potential to produce high amounts of astaxantin. However, this species has not been detected molecularly previously. This study aimed to obtain the morphological and molecular characters of microalgae isolates from BBPBAP Jepara using 18S rDNA to ascertain the species and its potential development. The stages of the research carried out include; microalgae cultivation, growth pattern observation, morphological observation, DNA isolation, amplification using 18S rDNA markers, electrophoresis, sequence data analysis, and phylogenetic using NJ Plot and MEGA applications 7. Results of morphological characterization of the Jepara BBPBAP showed that cell of microalgae isolates had a round shape, green color with a size of 4.5μm, has no flagella and passive motility. The results of the molecular characterization using 18S rDNA showed that isolate BBPBAP had highest similarity with Chlorella sorokiniana about 99%.
KONSTRUKSI PLASMID PRHA SEBAGAI PEMBAWA GEN ARAA PENYANDI ENZIM L-ARABINOSA ISOMERASE DARI Thermotoga thermarum M Masfuroh; Anto Budiharjo; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.797 KB)

Abstract

D-tagatosa as natural low-calorie sweeterners is likely to be a sugar substitute for diabetics type II. D-tagatosa sweetness levels is by 92% the sweetness of sucrose, but only 38% of calories of sucrose. This study aimed to obtain a recombinant plasmid construction pRHA::araA. Results subcloning was then used in biotransformation processes produce D-tagatosa. Propagation vector soure pRHA   was done with the cloning procedure in E. coli TOP’10. The process  used the vector source cut with enzyme NcoI and XhoI and producing pRHA vectore for gene insertion araA. AraA gene amplification was done using Polymerase Chain Reaction (PCR)  with  primers of AITth-For and AITth-Rev. Ligation was done using T4 ligase enzyme and transformed in E. coli TOP’10 by heat-shock methods. E. coli was grown in LB medium Agar with ampicillin concentration of 100 mg/ml. Selection was made on a liquid LB and LB Agar with ampicillin concentration range of 100-200 mg/ml. Based on the result of electrophoresis visualization of the pRHA::araA recombinant plasmid isolation were negative. Keyword:  L-arabinosa isomerase, araA, D-tagatosa, T. thermarum, E. coli
EKSTRAKSI DAN UJI STABILITAS ZAT WARNA DAUN JAMBU BIJI (Psidium guajava L.) Lasria Pardede; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.396 KB)

Abstract

Guava leaves is known as a traditional medicine to treat various diseases, such as diarrhea, dengue fever, etc. Along with the development of technology, guava leafs is now used as a color contributor on  textiles. This study aims to extract on the solvent distilled water and ethanol and to test the stability of guava leaf color pigment against the influence of storage temperature, time span of the addition of an oxidant and  pH. Leaves extracted by soaked for 24 hours in distilled water solvent that has been preheated to 30°C, 50ºC, 70º C and 90ºC and ethanol with a concentration of 20%, 40%, 60%, 80% and 96%. Absorbance measurements of guava leaf extract is using spectrophotometer at a wavelength of 525 nm. The results  showed that guava leaf extract has the optimum absorbance value on distilled water solvent at 90ºC and 20%ethanol. Stability test is done by storage temperature effect, oxidizing agents adding, and pH treatment. Stability test of guava leaf extract showed that extracted guava leaf color pigment is stable on 9°C storage temperature, short time oxidant adding (3 hours), and resistant to alkaline pH conditions (pH 9).Keywords: Psidium guajava L., ethanol, distilled water, spectrophotometry
BIOPROSPEKSI BAKTERI YANG BERASOSIASI DENGAN ALGA HIJAU HALIMEDA MACROLOBA, CAULERPA RACEMOSA, DAN ULVA SP SEBAGAI PENGHASIL SENYAWA ANTIBAKTERI Risky Panji Nugroho; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.384 KB)

Abstract

Bacteria can grow in various environments, including those associated with marine organisms such as algae, sponge, sea grass and soft corals. For these organisms, bacteria help building their defense by producing secondary metabolites such as bioactive compound.  This research aims to study the potency of the bacteria which interact with green algae Halimeda macroloba, Caulerpa racemosa, and Ulva sp which are able to produce antibacterial compound. Algae, which belong to Chlorophyta, are common to be found in marine water. This research used bacterial isolation, morphologic bacterial isolate characterization, antibacterial test, molecular identification of the antibacterial compound producer isolate, and biochemical activity test. From the isolation, the researcher was able to collect five bacterial isolate; one from H. macroloba, three from C. racemosa, and one isolate from Ulva sp. Of the five isolates, one isolate from C. racemosa can prevent the E.coli, and one isolate from H. macroloba can prevent E.coli and P.aeruginosa. Isolate derived from Halimeda macroloba have the biggest prevention zone ability, which is 18.1 mm, towards P.aeruginosa. Keywords: Antibacterial, Bacterial Association, Halimeda macroloba, Caulerpa racemosa,      Ulva sp
TRANSFORMASI DAN KLONING PLASMID PJ804:77539 PADA E.coli TOP’10 Siu S.S Langden; Anto Budiharjo; w wijanarka; Wien Kusharyoto
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.848 KB)

Abstract

Kloning dan transformasi vektor PJ804:77539 dilakukan dengan tujuan perbanyakan vektor pRHA pada sel bakteri E.coli TOP’10. Ekpresi vektorpRHA diharapkan dapat terjadi pada periplasma E.coli dan memberikan ekspresi berupa kemampuan resistensi terhadap Ampicillin. Ekspresi pada periplasma bertujuan untuk meminimalisir kerugian yang timbul pada sistem ekspresi di sitoplasma di antaranya tingkat ekspresi yang rendah, protein terpotong atau resiko kontaminasi. Sekresi protein rekombinan pada periplasma dapat meningkatkan aktivitas biologis serta tingkat kestabilan produk menjadi lebih tinggi. Proses isolasi protein yang diekspresikan pada periplasma  dapat dilakukan dengan perlakuan stress osmotik ringan sehingga menurunkan resiko kontaminasi protein sitoplasma. Ekspresi protein pada periplasma diarahkan oleh peptida sinyal pelB. Peptida sinyal bekerja menarik produk protein ke periplasma dengan cara berfusi dengan ujung N-terminal pada peptida yang terekspresi. Penanda selektif (selectable marker) yang terdapat pada PJ804::77539 merupakan Ampr, suatu penanda yang memampukan bakteri untuk resisten pada keberadaan antibiotik Ampicillin. Transformasi dilakukan sesuai dengan metode heat – shock dan diseleksi pada medium LB agar dan LB cair yang mengandung antibiotik Ampicillin dengan konsentrasi 100 mg/mL. Diperoleh koloni tumbuh pada medium yang mengandung Ampicillin dan dilakukan isolasi plasmid. Visualisasi hasil elektroforesis memperlihatkan adanya pita plasmid yang diisolasi dari E.coli TOP’10 pada gel elektroforesis.Kata kunci : Ampicillin, E.coli, pelB, periplasm dan pRHA
ANALISIS FILOGENETIK Curcuma zedoaria (TEMU PUTIH) BERDASARKAN GEN INTERNAL TRANSCRIBED SPACER (ITS) Yuliandini Pangestika; Anto Budiharjo; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.193 KB)

Abstract

Curcuma zedoaria belongs to Zingiberaceae family which has a local name white turmeric. This local name is not only used by C. zedoaria, but also used by C. mangga and Kaempferia rotunda. This problem leads to incorrect selection of ingredients, so that the therapeutic effect can not to achieved. Therefore phylogenetic analysis is important to differentiate three types of these plants. Phylogenetic analysis illustrated the taxonomic classification of organisms based on evolutionary history. Sequence which used in this study was Internal Transcribed Spacer (ITS). ITS flanked by the coding region of 18S, 5.8S and 26S rDNA on each unit in a series of chromosomes. Aimed of this study was to perform phylogenetic analysis of C. zedoaria which from Indonesia based on ITS gene sequences. Methods included were DNA isolation using Doyle and Doyle method (1987), ITS gene amplification using ITS1 and ITS4 primer, analysis of ITS gene sequences using Basic Local Aligment Search Tool (BLAST), and construction of phylogenetic trees using MEGA6 by neighbor-joining tree method and bootstrap method with 1000 replications. DNA isolation resulted DNA concentration of 853 ng/µl and purity value of 2.17. Amplification of  ITS region resulted in 700 bp product. Result of homology search showed C. zedoaria had homology with C. zedoaria voucher JLS 71432 clone 4 from Czezh Republic with 72 % homology and 3 % gap. Phylogenetic analysis showed that white turmeric collected in this study was C. zedoaria and different from C. mangga and K. rotunda. This study concluded that C. zedoaria different from C. zedoaria from the Czech Republic, however both were in a Curcuma monophyletic group.Keywords: Curcuma zedoaria, Internal Transcribed  Spacer,  phylogenetic, white turmeric
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI RUSIP IKAN TERI (Stolephorus sp.) Deverina Mergypta; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.505 KB)

Abstract

Rusip is a traditional food from Bangka-Belitung made from fresh anchovy with salt and palm sugar that is brooded for seven days. Rusip fermentation process is caused by microbial activity mainly from lactic acid bacteria. The purpose of this study was to isolate lactic acid bacteria from rusip, to characterize isolates obtained based on common characteristic of lactic acid bacteria, and to carry out proximate analysis of rusip anchovy. Isolation from rusip fermented food obtained eight bacterial isolates, five isolates were circular and three isolates were rod-shaped. Characterization and identification of lactic acid bacterial were performed using tests that led to the common characteristic of lactic acid bacteria, i.e the gram staining, motility test, acid formation test, catalase test, and proteolytic test. The result showed that eight bacterial isolates were gram-positive, non-motile, catalase-negative, positive to produce acid, and had proteolytic activity. Rusip anchovy had 0,64% of total lactic acid and the pH 5,40. Nutrient content of rusip fermentation had 10,3644% moisture content; 50,0702% ash content; 0,9524% crude fat content; 0,3764% crude fiber content; 34,8603% crude protein content; and 14,1171% carbohydrate content. Keywords: rusip, isolation, characterization, lactic acid bacteria, proximate analysis
BIOPROSPEKSI RHIZOBAKTERI PENGHASIL IAA (Indole Acetic Acid) DARI TANAMAN JAGUNG (Zea maysL.) DI AREA PERTANIAN SEMI ORGANIK DESA BATURKEC. GETASAN KAB. SEMARANG Khoirul Huda; Anto Budiharjo; Budi Raharjo
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.977 KB)

Abstract

Microorganisms in nature have a rich diversity and have an important role in human lives, especially in agriculture. Some types of bacteria live in the area of plant roots called rhizobacteria. Some rhizobacteria has the ability to stimulate the growth of crops such as produce IAA (Indole Acetic Acid). This study aims to find rhizobacteria in maize (Zea mays L.) which has the ability to produce IAA (Indole Acetic Acid) that can be used as a reference in rhizobacteria resource utilization to increase agricultural production in a sustainable and environmentally friendly.This study was conducted with bacterial isolation, characterization of bacterial isolates in colony and cell morphology, The test of rhizobacteria’s ability to produce IAA (Indole Acetic Acid), the molecular identification of the isolates were able to produce IAA rhizobacteria and confirmatory tests. The results of isolation obtained seventeen isolates of rhizobacteria where there is a one of isolate (J.6) is able to produce 20.5 ppm IAA in TSB (Tryptic Soy Broth) supplemented with 100 ppm of L-tryptophan. The Result of the molecular identification show that isolate which has the ability to produce IAA has 97% similarity with Bacillus safensis Strain A-2. Isolate “J.6” have the same characteristic features with Bacillus safensis. They are a gram-positive, rod-shaped, capable of forming endospores, motile, catalase positive, capable of fermenting glucose and hydrolyzing a starch. Key word : Rhizobacteria, Indole Acetic Acid, Bacillus safensis Strain A-2
KARAKTERISASI MOLEKULER TANAMAN SAMBUNG NYAWA (Gynura procumbens [Lour.] Merr) BERDASARKAN 18S rRNA Octavia Dewi Christiningrum; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.529 KB)

Abstract

Sambung Nyawa (Gynura procumbens) is a traditional medicinal plant used by people as an antihyperglycemic drug, antihyperlipidemic and chemoprevention agents. Comprehensive information on this plant needs to be done, including DNA analysis, so it can complement morphological character of an organisms. This study aimed to molecularly characterize and determine the relationship between G. procumbens with other organisms using ribosomal 18S rRNA gene. PCR (Polymerase Chain Reaction) was used to amplify the 18S rRNA gene fragments G. procumbens. DNA sequences were analyzed using the BLAST program at the NCBI portal and construction of phylogenetic trees were tested using Bootstrap method 1000x. The results showed the 18S rRNA sequence of Gynura procumbens fragments was 1600 bp, and G. procumbens belonged in the family of Asteraceae.Key words : Gynura procumbens, PCR (Polymerase Chain Raction), 18S rRNA, Bootstrap method
Co-Authors AB SUSANTO Abhinaya, Danendra Daryl Ade Fajrian Adila Nawan Hasrimi Agung Suprihadi Agung Suprihadi Ahmad Thontowi Anggoro, Naufal Sebastian Aninditia Sabdaningsih Ariani, Nur Frida Asgasatya, Syahid Fattah Barri Pratama Budi Raharjo Bunga Fajriani CATUR RIANI Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Deverina Mergypta Dian Wahyu Kemalaputri Dudi Hardianto, Dudi Dyah Ayu Riani Dyah Wulandari Edwin Setiawan Elfira, Yolanda Elke Gildantia Elvi Yetti Endang Kusdiyantini Endang Kusdiyantini Fahmi, Maya Fitriana Ilul Fauziah Citra Rahmawati Fitri Arum Sasi, Fitri Arum Fitria, Lutfiah Rahmadini Florentina Kusmiyati HARTAJANIE, LAKSMI Hasrimi, Adila Nawan Heni Wijayanti, Heni Hermanto, Michael Einstein Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Husna, Faza Laili IS HELIANTI Khoirul Huda Kristina Kristina Kurniawati, Mufida Budi Kusumaningrum, Hermin Laila Nur Faizah Lasria Pardede Lindayani, Lindayani Luthfy AN M Masfuroh Maerani Sumarno Mahmudah, Hawari Rosdiana Maria, Atina Maulana, Anand Reyna Mawarni, Risa Arum Maya Fitriana Ilul Fahmi MG Isworo Rukmi Mia Tri Wardani Monalita, Ramadhebi Muhammad Adib Mubarok Muhammad Mulyadi, Muhammad Muhammad Zainuri Norma Sainstika Pangestu Novita Rahmawati, Novita Nur Endah Wahyuningsih Nurhayati Nurhayati Nurlita Abdulgani Octavia Dewi Christiningrum Olivia Nisa Mumtianah Pardede, Lasria Prayogo, Fitra Adi Rahayu, Annisa Rizky Rahmah Putri Sunarno Ratna Cempaka Lingga Rejeki Siti Ferniah Risky Panji Nugroho Risma Wiharyanti Safirah, Dearesty Saniha Adini, Saniha Sayono Setyo Nugroho Shabrina, Jauhara Siti Nur Jannah Siti Nur Jannah Siu S.S Langden Sonny Abdi Setiyawan, Sonny Abdi Soowanayan, Chumporn Sri Pujiyanto Suetrong, Satinee Sumarno, Maerani Suprihadi Susiana Purwantisari Teuku Tajuddin Thu'ti Alawiyyah Tony Hadibarata, Tony w wijanarka Wahyu Dewi U. Haryanti Wien Kusharyoto Wijanarka Wijanarka Yason Lukman Sudjito, Yason Lukman Yohanes Chandrawijaya Yopi Yopi Yuliandini Pangestika