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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Current Biochemistry Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Greensphere: Journal of Environmental Chemistry Prosiding Seminar Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Anto Budiharjo
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Veterinary

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Profiling of Seawater Bacterial Diversity in Tanjung Mas Port Using 16S rRNA eDNA Metabarcoding and Next-Generation Sequencing (NGS) Anggoro, Naufal Sebastian; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto; Wijanarka, Wijanarka; Zainuri, Muhammad; Hadibarata, Tony
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 1 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.1.61-70

Abstract

Tanjung Emas Port is the entry and exit point for trade commodities, both regional and international filled with many ships. This condition makes biofouling a very massive process in that place by various types of marine bacteria. The initial formation of a biofilm is relevant to bacterial diversity, colonization and adhesion. The objective of the study was assessing bacterial diversity in relation to with biofouling within Tanjung Mas Port Semarang, by using 16S rRNA eDNA metabarcoding Next-Generation Sequencing (NGS). Seawater samples from aquatic sites of Tanjung Mas harbor was used for DNA extraction and amplification of the 16S rRNA V-3-V4 hypervariable region, followed by sequencing and library construction of eDNA Metabarcoding. Sequence processing and analysis was performed in QIIME 2 and RStudio using DADA2 for advanced sequencing processing and Phyloseq. The results of this research showed that bacteria is the predominant taxon constituting 100% of the community. The taxon consists of Proteobacteria (49.38%), Bacteroidota (8.67%), and Firmicutes (8.88%). Alphaproteobacteria (20.92%) and Gammaproteobacteria (12.39%) dominate at the Class level, emphasizing their versatility and ecological influence. At the Order and Family levels reveals the prevalence of Rhodobacterales (10.04%), Chitinophagales (2.53%), Rhizobiales (3.61%), Rhodobacteraceae (5.67%), Saprospiraceae (3.63%), and Rhizobiaceae (2.15%). It was found that the unculturable taxa dominance in Tanjung Mas Port was 44.66%. These taxonomic entities contribute significantly to the taxonomic and functional diversity of the microbial community, influencing nutrient cycling, organic matter degradation, ecosystem stability and biofilm formation.
A Mini Review on Analysis of Potential Antibacterial Activity of Symbiotic Bacteria from Indonesian Freshwater Sponge: An Unexplored and A Hidden Potency Setiawan, Edwin; Hermanto, Michael Einstein; Abdulgani, Nurlita; Prasetyo, Endry Nugroho; Riani, Catur; Wulandari, Dyah; Budiharjo, Anto
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.82682

Abstract

Marine sponges have been investigated as potential bioresources because of their symbiotic relationship with microbes such as Actinobacteria that produce antibacterial substances. In contrast, a group of sponges, that inhabits freshwater environments called freshwater sponges (Order Spongillida Manconi & Pronzato, 2002) and consists of only one percent among all of the sponges’ species (Phylum Porifera Grant, 1836), has  not yet intensively examined.  For this reason, we screened, determined, evaluated, and reviewed by examining several databases in Scopus, Pub Med, and Google Scholar related to potential aspects of symbiotic bacteria and their antibacterial substances that can be further utilised  and developed into synthesised  antibacterial compounds, based on published metagenomic data of symbiotic bacteria in freshwater sponges. At the same time, we compared a composition of those freshwater symbionts to marine sponges’ symbionts whether those possess a similar composition or not. Moreover, a current report and a revisit study of freshwater sponges in East Java, initiate further direction on mapping of those symbiotic bacteria from Indonesia that can be nominated as potential groups possessing antibacterial properties. 
Bioprospecting and Molecular Identification of Amylase and Cellulase Producing Thermophilic Bacteria from Sediment of Nglimut Hot Springs, Kendal Regency Budiharjo, Anto; Wulandari, Dyah; Shabrina, Jauhara; Mawarni, Risa Arum; Maulana, Anand Reyna; Nurhayati, Nurhayati; Wijanarka, Wijanarka; Hartajanie, Laksmi; Lindayani, Lindayani
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86756

Abstract

The utilisation of enzymes in the industry has brought numerous benefits and advantages to production processes. Enzymes serve as biocatalysts, efficiently catalyzing reactions and hydrolysis in biochemical processes. However, there are challenges in applying enzymes in the industry, particularly concerning enzyme stability. The obstacle encountered in the production processes involving industrial enzyme applications is the low stability of enzymes when used at high temperatures. Heat-sensitive enzymes undergo damage or denaturation. Thermophilic microorganisms are chosen because they hold the potential to produce thermophilic enzymes. The thermophilic enzymes exhibit better heat stability compared to other enzymes, making them an effective alternative for future industrial production processes. This study aims to isolate thermotolerant bacteria from Nglimut Hot Spring sediment, screen for cellulase- and amylase-producing isolates, and molecularly identify the best isolate using 16S rRNA barcode. The results show that 22 bacterial isolates were found in the sediment of a hot spring; TS-14 was the best isolate in producing amylase, with the highest average amylolytic index of 2.38, whereas TS-15 had the highest cellulolytic index of 2.11. Based on 16S rRNA identification, TS-14 showed an homological identity of 79% with Bacillus amyloliquefaciens, while TS-15 had a 100% homological identity with Bacillus licheniformis. These results were important as the first step of screening bacterial potential to produce thermophilic enzymes that could be applied in the downstream processing in future industrial and biotechnology companies. 
MOLECULAR CHARACTERIZATION, PHYTOCHEMICALS SCREENING, AND MOLECULAR DOCKING OF CARDAMOM (Wurfbainia compacta), AND SAMBILOTO (Andrographis paniculata) AGAINST COVID-19 Kurniawati, Mufida Budi; Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jbbi.11.2.3005

Abstract

Cardamom and Sambiloto are phytopharmaceutical plants that produce phytochemical compounds that have the potential to be used to increase immunity against COVID-19. because they contain carotenoids, phenols, anthocyanins, saponins, alkaloids and steroids. This research aims to obtain the molecular characteristics of Cardamom and Sambiloto plants from the Gunungpati area, Semarang by ITS primer, testing phenolic phytochemicals, tannins, flavonoids, saponins and alkaloids followed by molecular docking tests with the 6WX4 protein. SARS-CoV-2. Molecular characterization results show that Cardamom and Sambiloto are similar to Wurfbainia compacta MF802556.1 (100%) and Andrographis paniculate LC646073.1 (84.47%). The results of the phytochemical test screening showed that both plants contain flavonoids. Molecular docking tests were carried out with the compounds Quercetin, Avicularin, Naringenin, 5-hydroxy-7,8,2',5' tetramethoxyflavone, and Retinoic Acid. Retinoic Acid as a test ligand has the greatest potential in inhibiting the 6WX4 protein in the SARS-CoV-2 virus with a binding affinity value of -7.28 and RMSD 0.00.
Deteksi Gen DXS dan Penentuan Jalur Biosintesis Karotenoid pada Chlorella pyrenoidosa Monalita, Ramadhebi; Kusumaningrum, Hermin; Budiharjo, Anto
Berkala Bioteknologi Vol. 2 No. 2 November 2019
Publisher : Berkala Bioteknologi

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Abstract

Sintesis karotenoid alami belum pernah melebihi produk sintetik pada skala komersial. Kurangnya pemahaman mengenai aspek mikrobiologis dan ekofisiologis isolat penghasil karotenoid menyebabkan terjadinya kesalahan penamaan spesies. Satu isolat lokal alga hijau yang digunakan sebagai pakan alami sumber karotenoid dalam industri makanan yaitu Chlorella pyrenoidosa. Akumulasi karotenoid jalur non- MVA pada alga hijau ditentukan oleh enzim D-1-deoksixilulosa 5-fosfat sintase, yang disandi oleh gen D- 1-deoksixilulosa 5-fosfat sintase (DXS). Tujuan utama penelitian ini adalah untuk mendeteksi gen DXS sebagai penyandi enzim kunci biosintesis karotenoid pada C. pyrenoidosa apakah juga mengikuti jalur non- MVA untuk biosintesis karotenoidnya atau tidak. Metode karakterisasi morfologis dan ekofisiologis dilakukan berdasarkan pengamatan secara berkala dan deteksi gen DXS menggunakan panduan Kuzuyama (2000). Hasil analisis keserupaan gen DXS C. pyrenoidosa pada daerah lestari memperlihatkan dapat mendeteksi parsial gen DXS dari Chlamydomonas reinhardtii. Tidak adanya hambatan pertumbuhan pada C. pyrenoidosa dengan lovastatin menunjukkan jalur non-MVA adalah jalur yang digunakan dalam biosintesis karotenoidnya.
POTENSI RIZOBAKTERI PEMBENTUK ENDOSPORA DARI TANAMAN PADI SEBAGAI BIOKONTROL FITOPATOGEN Xanthomonas oryzae Sumarno, Maerani; Budiharjo, Anto; Pujiyanto, Sri
Berkala Bioteknologi Vol. 2 No. 2 November 2019
Publisher : Berkala Bioteknologi

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Abstract

Xanthomonas oryzae adalah fitopatogen penyebab hawar daun bakteri yang mengakibatkan penurunan hasil pertanian rata-rata mencapai 20-70 % di wilayah Asia. Gejala hawar daun bakteri ditandai dengan terbentuknya garis pada helaian daun yang berubah menjadi kuning, kemudian putih hingga menyebabkan tanaman menjadi layu dan mati. Rizobakteri pembentuk endospora merupakan mikroba tanah yang berpotensi sebagai biokontrol dalam menghambat pertumbuhan fitopatogen. Tujuan penelitian ini adalah diperolehnya isolat rizobakteri pembentuk endospora dari tanaman padi sebagai biokontrol terhadap fitopatogen X. oryzae. Metode yang digunakan meliputi, isolasi, uji aktivitas antibakteri, identifikasi secara molekuler, dan karakterisasi secara biokimia. Rizobakteri pembentuk endospora yang diperoleh dari hasil isolasi tanaman padi sebanyak dua puluh isolat. Isolat P-10 merupakan isolat yang mempunyai daya hambat terbesar terhadap X. oryzae dengan zona hambat sebesar 18,89 mm. Hasil identifikasi molekuler menggunakan gen 16S rRNA menunjukkan isolat P-10 memiliki homologi 98% dengan Bacillus pumilus. Karakterisasi biokimia menunjukkan isolat P-10 berbentuk batang dengan letak endospora di tengah, gram positif, positif menghasilkan katalase, bersifat motil, negatif dalam menghidrolisis pati, tidak membentuk gas pada glukosa, karakteristik tersebut sesuai dengan karakter B. pumilus.
Karakterisasi Potensi Bacillus altitudinis P-10 sebagai Plant Growth Promoting Rhizobacteria (PGPR) Husna, Faza Laili; Budiharjo, Anto; Purwantisari, Susiana
Berkala Bioteknologi Vol. 4, No. 1, April 2021
Publisher : Berkala Bioteknologi

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Abstract

Plant Growth Promoting Rhizobacteri (PGPR) memiliki kemampuan untuk meningkatkan pertumbuhan serta meningkatkan ketahanan terhadap hama dan penyakit pada tanaman. Bacillus altitudinis P-10 dari rhizosfer tanaman padi persawahan organik di Kecamatan Banyubiru, Kabupaten Semarang, Jawa Tengah memiliki potensi sebagai PGPR yang memiliki kemampuan menghambat fitopatogen Xanthomonas oryzae dan Xanthomonas campestris, serta diprediksi memiliki gen untuk produksi antibiotik peptida non ribosomal. Karakterisasi B. altitudinis P-10 dilakukan untuk mengetahui potensinya sebagai PGPR melalui uji kualitatif yang meliputi aktivitas kitinolitik dan selulolitik, produksi IAA (Indole Acetic Acid), produksi HCN (Hidrogen Sianida), kemampuan melarutkan fosfat dan produksi senyawa antibiotik. Hasil uji kualitatif menunjukkan B. altitudinis P-10 memiliki aktivitas selulolitik, kemampuan menghasilkan IAA dan kemampuan melarutkan fosfat serta produksi senyawa antibiotik dari golongan peptida non ribosomal yaitu bacitracin, gramicidin, surfactin, fengycin dan bacillomycin D. Bacillus altitudinis P-10 memiliki karakteristik sebagai PGPR melalui kemampuannya dalam memproduksi IAA, melarutkan fosfat, aktivitas selulolitik dan produksi antibiotik. Hal tersebut menunjukkan potensi B. altitudinis P-10 sebagai PGPR.
EKSTRAKSI DAN UJI STABILITAS ZAT WARNA DAUN JAMBU BIJI (Psidium guajava L.) Pardede, Lasria; Kusdiyantini, Endang; Budiharjo, Anto
Berkala Bioteknologi Vol. 2 No. 2 November 2019
Publisher : Berkala Bioteknologi

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Abstract

Daun jambu biji dikenal sebagai obat tradisonal untuk mengobati berbagai macam penyakit, seperti diare, demam berdarah, dll. Seiring perkembangan teknologi daun jambu biji kini digunakan sebagai penyumbang warna pada tekstil. Penelitian ini bertujuan untuk mengekstraksi pada pelarut aquades dan etanol serta menguji stabilitas warna daun jambu biji terhadap pengaruh suhu penyimpanan, rentan waktu penambahan oksidator dan pH. Daun diekstrak dengan direndam selama 24 jam pada pelarut aquades yang telah dipanaskan terlebih dahulu pada suhu 30ºC, 50ºC, 70ºC dan 90ºC dan etanol dengan konsentrasi 20%, 40%, 60%, 80% dan 96%. Pengukuran absorbansi ekstrak daun jambu biji menggunakan spektrofotometri pada panjang gelombang 525 nm. Hasil menunjukkan bahwa ekstrak daun jambu biji mempunyai nilai absorbansi optimum pada pelarut aquades pada suhu 90ºC dan etanol 20%. Uji stabilitas dilakukan dengan perlakuan pengaruh terhadap suhu penyimpanan, penambahan oksidator dan pH. Uji stabilitas ekstrak daun jambu biji menunjukkan bahwa zat warna daun jambu biji yang diekstrak stabil pada suhu penyimpanan 9°C, penambahan oksidator dengan waktu yang singkat (pada jam ke 3) dan tahan pada kondisi pH basa (pH 9).
Isolasi dan Identifikasi Molekuler Bakteri Pelarut Fosfat dari Gua Gamelan di Kawasan karst Kiskendo Kendal, Jawa Tengah Mahmudah, Hawari Rosdiana; Suprihadi, Agung; Budiharjo, Anto
Berkala Bioteknologi Vol. 2 No. 2 November 2019
Publisher : Berkala Bioteknologi

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Abstract

Bakteri pelarut fosfat (BPF) merupakan bakteri yang mampu melarutkan fosfat yang tidak tersedia menjadi tersedia sehingga dapat diserap oleh tanaman. Penelitian ini bertujuan untuk melakukan isolasi dan identifikasi molekuler bakteri pelarut fosfat melalui identifikasi 16s rRNA dari guano gua gamelan dan kemampuannya dalam melarutkan fosfat. Kegiatan penelitian ini dilakukan dalam beberapa tahap, yaitu isolasi BPF dalam medium pikovskaya dan NBRIP, pewarnaan gram, serta identifikasi molekuler. Hasil isolasi diperoleh dua isolat bakteri, yaitu G4-1 dan G5. Isolat G5 yang paling tinggi melarutkan fosfat dengan diameter zona bening sebesar 22.01 mm, dan isolat G4-1 memiliki diameter zona bening 19.8 mm. Isolat G4-1 dan G5 merupakan bakteri gram- negatif. Hasil amplifikasi DNA BPF menggunakan primer universal 27 F (5’-AGAGTTTGATCMTGGCTCAG- 3’) dan Primer 1492 R (5’-GGTTACCTTGTTACGACTT-3’) menghasilkan produk PCR berukuran 1520 bp dan 1235 bp. Hasil analisis sekuen gen 16s rRNA menunjukan bahwa isolat G4-1 memiliki homologi sebesar 80% dengan Acinetobacter iwofii strain-JCM parsial sekuen, sedangkan isolat G5 memiliki homologi sebesar 92% dengan Serratia marcesens strain-NBRC
EFEKTIVITAS KRIM FORMULASI TEH HIJAU (Camellia sinensis) SEBAGAI AGEN ANTIBAKTERI TERHADAP PATOGEN KULIT Prayogo, Fitra Adi; Wijayanti, Heni; Rahmawati, Novita; Budiharjo, Anto; Wulandari, Dyah
Greensphere: Journal of Environmental Chemistry Vol 5, No 1 (2025): Volume 5 Nomor 1 Tahun 2025
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/gjec.2025.26815

Abstract

This research aims to determine the effect of green tea extract cream 6%, 8%, and 10% w/w against Staphylococcus epidermidis and Propionibacterium acne. The method begins with making green tea extract using the maceration method, and qualitative flavonoid, alkaloid, saponin, and tannin tests are carried out. Next, S. epidermidis and P. acnes were rejuvenated on NA media and cultured at 37°C for 24 hours. Both bacteria were then inoculated into different test tubes containing 0.9% NaCl. The suspension was then vortexed, and the turbidity level was equalized with McFarland's solution. Both bacteria were then cultured on NA media, and paper disks that had previously been soaked in clindamycin (control +) and 0%, 6%, 8%, and 10% green tea extract cream were placed on the agar media. The culture was then incubated for 24 hours at 37°C, and the inhibition zone was checked with a caliper. Results show that green tea extract has positive tests for flavonoids, alkaloids, saponins, and tannins. The optimum concentration of extract cream on antibacterial activity against S. epidermidis and P. acne is 10%. Inhibition zone comparison showed a significant difference between the diameter of the inhibition zone of S. epidermidis and P. acne, p = 0,00485 (p < 0,05), which means that green tea extract cream is better at fighting P. acne.
Co-Authors AB SUSANTO Abhinaya, Danendra Daryl Ade Fajrian Adila Nawan Hasrimi Agung Suprihadi Agung Suprihadi Ahmad Thontowi Anggoro, Naufal Sebastian Aninditia Sabdaningsih Ariani, Nur Frida Asgasatya, Syahid Fattah Barri Pratama Budi Raharjo Bunga Fajriani CATUR RIANI Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Deverina Mergypta Dian Wahyu Kemalaputri Dudi Hardianto, Dudi Dyah Ayu Riani Dyah Wulandari Edwin Setiawan Elfira, Yolanda Elke Gildantia Elvi Yetti Endang Kusdiyantini Endang Kusdiyantini Fahmi, Maya Fitriana Ilul Fauziah Citra Rahmawati Fitri Arum Sasi, Fitri Arum Fitria, Lutfiah Rahmadini Florentina Kusmiyati HARTAJANIE, LAKSMI Hasrimi, Adila Nawan Heni Wijayanti, Heni Hermanto, Michael Einstein Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Husna, Faza Laili IS HELIANTI Khoirul Huda Kristina Kristina Kurniawati, Mufida Budi Kusumaningrum, Hermin Laila Nur Faizah Lasria Pardede Lindayani, Lindayani Luthfy AN M Masfuroh Maerani Sumarno Mahmudah, Hawari Rosdiana Maria, Atina Maulana, Anand Reyna Mawarni, Risa Arum Maya Fitriana Ilul Fahmi MG Isworo Rukmi Mia Tri Wardani Monalita, Ramadhebi Muhammad Adib Mubarok Muhammad Mulyadi, Muhammad Muhammad Zainuri Norma Sainstika Pangestu Novita Rahmawati, Novita Nur Endah Wahyuningsih Nurhayati Nurhayati Nurlita Abdulgani Octavia Dewi Christiningrum Olivia Nisa Mumtianah Pardede, Lasria Prayogo, Fitra Adi Rahayu, Annisa Rizky Rahmah Putri Sunarno Ratna Cempaka Lingga Rejeki Siti Ferniah Risky Panji Nugroho Risma Wiharyanti Safirah, Dearesty Saniha Adini, Saniha Sayono Setyo Nugroho Shabrina, Jauhara Siti Nur Jannah Siti Nur Jannah Siu S.S Langden Sonny Abdi Setiyawan, Sonny Abdi Soowanayan, Chumporn Sri Pujiyanto Suetrong, Satinee Sumarno, Maerani Suprihadi Susiana Purwantisari Teuku Tajuddin Thu&#039;ti Alawiyyah Tony Hadibarata, Tony w wijanarka Wahyu Dewi U. Haryanti Wien Kusharyoto Wijanarka Wijanarka Yason Lukman Sudjito, Yason Lukman Yohanes Chandrawijaya Yopi Yopi Yuliandini Pangestika