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DNA damage and shell malformation in Blue Mussel, Mytilus edulis Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 6, No 1 (2022)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.6.1.65-74

Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour.Â The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters.Â DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro.Â The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site.Â Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.

Microplastic: pollution issue and seafood security Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.25-27

At the first time, plastic materials were produced to facilitate various activities of human life. Plastic materials that are flexible and durable have been used by humans to meet various needs to support their daily activities. Starting from human activities from waking up to going back to sleep, nowadays it cannot be separated from the use of plastic materials. This then makes humans "addicted" to plastic materials. It is as if human life cannot be separated from the use of plastic materials. Various research results in the field of pollution both on land and the sea, plastic materials that are not managed properly can contaminate human life, either directly or indirectly, to food sources, especially food from the sea.

Effectiveness of clove oil as anesthesia of Sergeant Major Abudefduf vaigiensis (Quoy & Gaimard, 1825) Ainun Ayu Utami Amris; Sri Wahyuni Rahim; Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 4, No 1 (2020)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.4.1.21-28

The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time Â and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).

Nutrient Absorption Rate of Oryzias celebensis Embryo Yulia Indah Sari Lalombo; Khusnul Yaqin; Sharifuddin bin Andy Omar
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.67-71

Oryzias celebensis is a species of medaka fish in South Sulawesi. During incubation, the growth and development of these fish embryos depend on the nutrients stored in the eggs. Therefore, this study aimed to determine the volume size of egg yolks and oil droplets and the absorption rate of egg yolks of O. celebensis embryos that reared in different incubation media. The study was conducted using a completely randomized design (CRD) with four rearing media treatments, namely A (Early Rearing Media), B (bottled water), C (Pattunuang river water) and D (well water) with five replications . The results showed that the volume of egg yolk and O. celebensis embryo oil in each medium showed a decrease in volume size along with the embryonic development stage. The absorption rate of yolk in O. celebensis embryos in media A had the biggest yolk absorption with an average 0.015±0.038 mm3 while media D had the smallest yolk absorption with an average 0.011±0.022 mm3.

Microplastics contamination in green mussels Perna viridis in Pangkajene Kepulauan Waters, South Sulawesi, Indonesia Ramli; Khusnul Yaqin; Nita Rukminasari
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.1-5

Microplastics is a problem that has been concerning, especially in marine habitat. The presence of microplastics in large quantities will have an impact on the environment and marine organisms. Marine organisms that are vulnerable to contamination by microplastics pollutants namely green mussels (Perna viridis). This study aims to determine the microplastics forms and concentration found in green mussels (Perna viridis) in Maccini Baji Waters, Labakkang District, Pangkejene Kepulauan Regency, South Sulawesi. The sampling of the mussels was carried out by the method of purposive random sampling with the sample was grouped into 3 groups of shells lengths, namely 2-3.9 cm, 4-5.9 cm and 6-7.9 cm with 33 individuals for each group. Microplastics observations were carried out using a stereo microscope. In addition to analyze microplastics concentrations, an analysis of the frequency of microplastics presence in shells was also carried out. The results showed that the microplastics was found in the form of fiber and fragments with several colors namely clear white, blue, black, red, and purple. The frequency of microplastics presence in green mussels was above 50% with an average of 71.7% exposure to microplastics. The highest frequency of microplastics presence and concentration in the range of shell lengths accounting for 2-3.9 cm.

DNA damage and shell malformation in Blue Mussel, Mytilus edulis Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 6, No 1 (2022)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.6.1.65-74

Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour.Â The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters.Â DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro.Â The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site.Â Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.

Microplastic: pollution issue and seafood security Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.25-27

At the first time, plastic materials were produced to facilitate various activities of human life. Plastic materials that are flexible and durable have been used by humans to meet various needs to support their daily activities. Starting from human activities from waking up to going back to sleep, nowadays it cannot be separated from the use of plastic materials. This then makes humans "addicted" to plastic materials. It is as if human life cannot be separated from the use of plastic materials. Various research results in the field of pollution both on land and the sea, plastic materials that are not managed properly can contaminate human life, either directly or indirectly, to food sources, especially food from the sea.

Effectiveness of clove oil as anesthesia of Sergeant Major Abudefduf vaigiensis (Quoy & Gaimard, 1825) Ainun Ayu Utami Amris; Sri Wahyuni Rahim; Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 4, No 1 (2020)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.4.1.21-28

The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time Â and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).

Pengaruh Paparan Logam Timbal Terhadap Kematian Embrio Ikan Medaka Embrio (Oryzias celebensis Weber, 1894) di Indonesia Damayani, Indah Ashary; Kurniawan B, Agus; Yaqin, Khusnul; Irmawati
SIGANUS: Journal of Fisheries and Marine Science Vol 3 No 2 (2022): SIGANUS: Journal of Fisheries and Marine Science
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/siganus.v3i2.1507

Penelitian ini bertujuan untuk menentukan pengaruh paparan logam timbal terhadap embrio dan tingkat kelangsungan hidup Ikan Medaka. Laju proporsional mortalitas dan survival rate embrio ikan medaka yang terpapar logam Pb pada nilai yang berbeda pada konsentrasi 0,02 mg / l, 0,2 mg / l dan 2 mg / l. Pengumpulan data kematian dilakukan setiap jam selama 10 hari dengan mengamati jumlah telur yang mati disamping mengamati kematian juga tingkat kelangsungan hidup. Hasil analisis data mortalitas dengan nilai rata-rata dan standar deviasi didapatkan mortalitas embrio ikan medaka pada kontrol berbeda nyata dengan kelompok kontrol. Dampak toksikologi embrio lebih terlihat pada konsentrasi mortalitas yang lebih tinggi (2 mg/l) dibandingkan dengan konsentrasi yang lebih rendah (0,02mg / l). Insiden tertinggi sebesar konsentrasi deviasi standar (0,02mg / l). Hasil penelitian membuktikan bahwa semakin tinggi pengaruh konsentrasi logam Pb, maka kematian atau kelangsungan hidup embrio ikan medaka yang umum terjadi di perairan permukaan sungai pattunuangasue semakin rendah. A B S T R A C T The present study aimed to determine the mortality of embryos and the survival rate of medaka fish exposed to Pb metal. The proportional rate of mortality and survival rate of medaka fish embryos exposed to Pb metal at different values at concentrations of 0.02 mg/l, 0.2 mg/l and 2 mg/l. Mortality data collection was carried out every hour for 10 days by observing the number of dead eggs in addition to mortality also observed survival rates. Mortality data analysis results with the average value and standard deviation obtained mortality of medaka fish embryos at the control were significantly different from the control group. The embryo toxicological impact was more pronounced in higher mortality concentrations of (2mg/l) compared to lower concentrations (0.02mg/l). Their highest incidence amounted to standard deviation concentrations of (0,02mg/l). The study proved significant the higher the concentration of Pb metals impact, the mortality or the lower the survival rate of medaka fish embryos commonly occurring in surface waters in Pattunuangasue river.

The estimation of carbon storage in the seagrass meadows of Badi Island Baharuddin, Nurazizah Pratiwi; Yaqin, Khusnul; Mashoreng, Supriadi
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 7, No 2 (2023)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.7.2.117-124

Global warming is becoming increasingly apprehensive day by day, marked by the continued increase in levels of carbon dioxide (CO2) in nature. One of the efforts made to reduce the effects of global warming is biosequestration by empowering photosynthetic organisms, one of which is seagrass beds. This study aims to measure the density and percentage of seagrass cover, biomass value, and estimate carbon storage in seagrass biomass in the form of tissue at the top (leaves and upright stems) and tissue at the bottom (roots and rhizomes), as well as estimate carbon storage in sediments. Sampling was carried out using line transect and quadrant plot methods at each station. Calculation seagrass carbon storage using the Loss of Ignition (LOI) method at the Soil Chemistry Laboratory of the Pangkep State Agricultural Polytechnic, a total of 5 species of seagrass were found, namely Enhalus accoroides, Thalassia hemprichii, Halodule uninervis, Halopila ovalis, and Cymodocea rotundata. The average seagrass carbon storage on Badi Island is 18.92 gC/m2, or 0.189 MgC/ha. The species that act as the largest contributor to carbon storage are Cymodocea rotundata, namely 35.44 gC/m2, and Halopila ovalis, which acts as the lowest contributor to carbon storage, namely 1.92 gC/m2. The average seagrass carbon storage at the bottom of the substrate was three times greater than that at the top of the substrate, namely 14.10 gC/m2 at the bottom and 4.82 gC/m2 at the top. The average carbon storage in sediments is 10.98 gC/m2, or 0.109 MgC/ha.

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