Articles
DNA damage and shell malformation in Blue Mussel, Mytilus edulis
Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 6, No 1 (2022)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.6.1.65-74
Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour. The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters. DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro. The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site. Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.
Microplastic: pollution issue and seafood security
Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.5.1.25-27
At the first time, plastic materials were produced to facilitate various activities of human life. Plastic materials that are flexible and durable have been used by humans to meet various needs to support their daily activities. Starting from human activities from waking up to going back to sleep, nowadays it cannot be separated from the use of plastic materials. This then makes humans "addicted" to plastic materials. It is as if human life cannot be separated from the use of plastic materials. Various research results in the field of pollution both on land and the sea, plastic materials that are not managed properly can contaminate human life, either directly or indirectly, to food sources, especially food from the sea.
Effectiveness of clove oil as anesthesia of Sergeant Major Abudefduf vaigiensis (Quoy & Gaimard, 1825)
Ainun Ayu Utami Amris;
Sri Wahyuni Rahim;
Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 4, No 1 (2020)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.4.1.21-28
The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time  and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).
Nutrient Absorption Rate of Oryzias celebensis Embryo
Yulia Indah Sari Lalombo;
Khusnul Yaqin;
Sharifuddin bin Andy Omar
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.5.2.67-71
Oryzias celebensis is a species of medaka fish in South Sulawesi. During incubation, the growth and development of these fish embryos depend on the nutrients stored in the eggs. Therefore, this study aimed to determine the volume size of egg yolks and oil droplets and the absorption rate of egg yolks of O. celebensis embryos that reared in different incubation media. The study was conducted using a completely randomized design (CRD) with four rearing media treatments, namely A (Early Rearing Media), B (bottled water), C (Pattunuang river water) and D (well water) with five replications . The results showed that the volume of egg yolk and O. celebensis embryo oil in each medium showed a decrease in volume size along with the embryonic development stage. The absorption rate of yolk in O. celebensis embryos in media A had the biggest yolk absorption with an average 0.015±0.038 mm3 while media D had the smallest yolk absorption with an average 0.011±0.022 mm3.
Microplastics contamination in green mussels Perna viridis in Pangkajene Kepulauan Waters, South Sulawesi, Indonesia
Ramli;
Khusnul Yaqin;
Nita Rukminasari
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.5.1.1-5
Microplastics is a problem that has been concerning, especially in marine habitat. The presence of microplastics in large quantities will have an impact on the environment and marine organisms. Marine organisms that are vulnerable to contamination by microplastics pollutants namely green mussels (Perna viridis). This study aims to determine the microplastics forms and concentration found in green mussels (Perna viridis) in Maccini Baji Waters, Labakkang District, Pangkejene Kepulauan Regency, South Sulawesi. The sampling of the mussels was carried out by the method of purposive random sampling with the sample was grouped into 3 groups of shells lengths, namely 2-3.9 cm, 4-5.9 cm and 6-7.9 cm with 33 individuals for each group. Microplastics observations were carried out using a stereo microscope. In addition to analyze microplastics concentrations, an analysis of the frequency of microplastics presence in shells was also carried out. The results showed that the microplastics was found in the form of fiber and fragments with several colors namely clear white, blue, black, red, and purple. The frequency of microplastics presence in green mussels was above 50% with an average of 71.7% exposure to microplastics. The highest frequency of microplastics presence and concentration in the range of shell lengths accounting for 2-3.9 cm.
DNA damage and shell malformation in Blue Mussel, Mytilus edulis
Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 6, No 1 (2022)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.6.1.65-74
Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour. The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters. DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro. The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site. Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.
Microplastic: pollution issue and seafood security
Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.5.1.25-27
At the first time, plastic materials were produced to facilitate various activities of human life. Plastic materials that are flexible and durable have been used by humans to meet various needs to support their daily activities. Starting from human activities from waking up to going back to sleep, nowadays it cannot be separated from the use of plastic materials. This then makes humans "addicted" to plastic materials. It is as if human life cannot be separated from the use of plastic materials. Various research results in the field of pollution both on land and the sea, plastic materials that are not managed properly can contaminate human life, either directly or indirectly, to food sources, especially food from the sea.
Effectiveness of clove oil as anesthesia of Sergeant Major Abudefduf vaigiensis (Quoy & Gaimard, 1825)
Ainun Ayu Utami Amris;
Sri Wahyuni Rahim;
Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 4, No 1 (2020)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna
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DOI: 10.29239/j.akuatikisle.4.1.21-28
The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time  and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).
Pengaruh Paparan Logam Timbal Terhadap Kematian Embrio Ikan Medaka Embrio (Oryzias celebensis Weber, 1894) di Indonesia
Damayani, Indah Ashary;
Kurniawan B, Agus;
Yaqin, Khusnul;
Irmawati
SIGANUS: Journal of Fisheries and Marine Science Vol 3 No 2 (2022): SIGANUS: Journal of Fisheries and Marine Science
Publisher : Universitas Sulawesi Barat
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DOI: 10.31605/siganus.v3i2.1507
Penelitian ini bertujuan untuk menentukan pengaruh paparan logam timbal terhadap embrio dan tingkat kelangsungan hidup Ikan Medaka. Laju proporsional mortalitas dan survival rate embrio ikan medaka yang terpapar logam Pb pada nilai yang berbeda pada konsentrasi 0,02 mg / l, 0,2 mg / l dan 2 mg / l. Pengumpulan data kematian dilakukan setiap jam selama 10 hari dengan mengamati jumlah telur yang mati disamping mengamati kematian juga tingkat kelangsungan hidup. Hasil analisis data mortalitas dengan nilai rata-rata dan standar deviasi didapatkan mortalitas embrio ikan medaka pada kontrol berbeda nyata dengan kelompok kontrol. Dampak toksikologi embrio lebih terlihat pada konsentrasi mortalitas yang lebih tinggi (2 mg/l) dibandingkan dengan konsentrasi yang lebih rendah (0,02mg / l). Insiden tertinggi sebesar konsentrasi deviasi standar (0,02mg / l). Hasil penelitian membuktikan bahwa semakin tinggi pengaruh konsentrasi logam Pb, maka kematian atau kelangsungan hidup embrio ikan medaka yang umum terjadi di perairan permukaan sungai pattunuangasue semakin rendah. A B S T R A C T The present study aimed to determine the mortality of embryos and the survival rate of medaka fish exposed to Pb metal. The proportional rate of mortality and survival rate of medaka fish embryos exposed to Pb metal at different values at concentrations of 0.02 mg/l, 0.2 mg/l and 2 mg/l. Mortality data collection was carried out every hour for 10 days by observing the number of dead eggs in addition to mortality also observed survival rates. Mortality data analysis results with the average value and standard deviation obtained mortality of medaka fish embryos at the control were significantly different from the control group. The embryo toxicological impact was more pronounced in higher mortality concentrations of (2mg/l) compared to lower concentrations (0.02mg/l). Their highest incidence amounted to standard deviation concentrations of (0,02mg/l). The study proved significant the higher the concentration of Pb metals impact, the mortality or the lower the survival rate of medaka fish embryos commonly occurring in surface waters in Pattunuangasue river.
Konsep Hukum dalam Pelaksanaan Peralihan Hak Atas Tanah Karena Jual Beli
Fathul Mujib Al-Mursyidi;
Khusnul Yaqin
ALADALAH: Jurnal Politik, Sosial, Hukum dan Humaniora Vol. 2 No. 2 (2024): Jurnal Politik, Sosial, Hukum dan Humaniora
Publisher : Sekolah Tinggi Ilmu Syariah Nurul Qarnain Jember
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DOI: 10.59246/aladalah.v2i2.814
It cannot be denied that in everyday people's lives there is still a lot of buying and selling of land that is done underhand. A land sale and purchase agreement that is not made before an Acting Land Deed Maker (PPAT) can cause losses to the buyer, for example the act of land sale and purchase in the case of Decision No. 34/Pdt.G/2020/PN.Dmk. The aim of this research is to answer the problem regarding the legal strength of land sale and purchase agreements that were not made before the Acting Land Deed Maker (PPAT) and to analyze the legal considerations of the Demak District Court judge's decision regarding No. 34/Pdt.G/2020/PN.Dmk. The results of this research show that 1) The legal strength of private deeds in sales and purchase agreements for land with ownership certificates in decision no. 34/Pdt.G/2020/PN.Dmk is valid and has legal force. This is proven by the positive arguments of the lawsuit, evidence and facts confirmed by the parties. 2) Analysis of the decision in case No. 34/Pdt.G/2020/PN.Dmk, the panel of judges gave permission to the Plaintiff to appear before the PPAT to sign the Deed of Sale and Purchase of land rights, granting the plaintiff's claim in part with verstek using the balance theory. The judge also carefully reviewed the contents of article 125 HIR, especially points 3 and 5, by deciding to grant the plaintiff's petitum not only based on the plaintiff's absence. The judge has also paid attention to the specifics of land sales and purchases regulated in the Basic Agrarian Law (UUPA). The authority to make certain authentic deeds, the difference is the legal basis that regulates the two. PPAT is Law Number 5 of 1960, Government Regulation Number 24 of 1997, Government Regulation Number 37 of 1998 and Regulation of the Head of the National Land Agency Number 1 of 2006, while Notary is Law Number 30 of 2004. However, in the provisions of Article 15 Paragraph (1) letter f of Law Number 30 of 2004 further strengthens the authority of Notaries in making deeds regarding land which are not deeds made by PPAT, especially deeds of land sale and purchase agreements.