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DNA damage and shell malformation in Blue Mussel, Mytilus edulis Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 6, No 1 (2022)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.6.1.65-74

Abstract

Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour.  The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters.  DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro.  The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site.  Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.
DNA damage and shell malformation in Blue Mussel, Mytilus edulis Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 6, No 1 (2022)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.6.1.65-74

Abstract

Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour.  The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters.  DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro.  The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site.  Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.
Effectiveness of clove oil as anesthesia of Sergeant Major Abudefduf vaigiensis (Quoy & Gaimard, 1825) Ainun Ayu Utami Amris; Sri Wahyuni Rahim; Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 4, No 1 (2020)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.4.1.21-28

Abstract

The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time  and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).
Nutrient Absorption Rate of Oryzias celebensis Embryo Yulia Indah Sari Lalombo; Khusnul Yaqin; Sharifuddin bin Andy Omar
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.67-71

Abstract

Oryzias celebensis is a species of medaka fish in South Sulawesi. During incubation, the growth and development of these fish embryos depend on the nutrients stored in the eggs. Therefore, this study aimed to determine the volume size of egg yolks and oil droplets and the absorption rate of egg yolks of O. celebensis embryos that reared in different incubation media. The study was conducted using a completely randomized design (CRD) with four rearing media treatments, namely A (Early Rearing Media), B (bottled water), C (Pattunuang river water) and D (well water) with five replications . The results showed that the volume of egg yolk and O. celebensis embryo oil in each medium showed a decrease in volume size along with the embryonic development stage. The absorption rate of yolk in O. celebensis embryos in media A had the biggest yolk absorption with an average 0.015±0.038 mm3 while media D had the smallest yolk absorption with an average 0.011±0.022 mm3.
Microplastic: pollution issue and seafood security Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.25-27

Abstract

At the first time, plastic materials were produced to facilitate various activities of human life. Plastic materials that are flexible and durable have been used by humans to meet various needs to support their daily activities. Starting from human activities from waking up to going back to sleep, nowadays it cannot be separated from the use of plastic materials. This then makes humans "addicted" to plastic materials. It is as if human life cannot be separated from the use of plastic materials. Various research results in the field of pollution both on land and the sea, plastic materials that are not managed properly can contaminate human life, either directly or indirectly, to food sources, especially food from the sea.
Microplastics contamination in green mussels Perna viridis in Pangkajene Kepulauan Waters, South Sulawesi, Indonesia Ramli Ramli; Khusnul Yaqin; Nita Rukminasari
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.1-5

Abstract

Microplastics is a problem that has been concerning, especially in marine habitat. The presence of microplastics in large quantities will have an impact on the environment and marine organisms. Marine organisms that are vulnerable to contamination by microplastics pollutants namely green mussels (Perna viridis). This study aims to determine the microplastics forms and concentration found in green mussels (Perna viridis) in Maccini Baji Waters, Labakkang District, Pangkejene Kepulauan Regency, South Sulawesi. The sampling of the mussels was carried out by the method of purposive random sampling with the sample was grouped into 3 groups of shells lengths, namely 2-3.9 cm, 4-5.9 cm and 6-7.9 cm with 33 individuals for each group. Microplastics observations were carried out using a stereo microscope. In addition to analyze microplastics concentrations, an analysis of the frequency of microplastics presence in shells was also carried out. The results showed that the microplastics was found in the form of fiber and fragments with several colors namely clear white, blue, black, red, and purple. The frequency of microplastics presence in green mussels was above 50% with an average of 71.7% exposure to microplastics. The highest frequency of microplastics presence and concentration in the range of shell lengths accounting for 2-3.9 cm.
INTERACTION OF CO2 AND LIGHT AVAILABILITY ON PHOTOPHYSIOLOGY OF TROPICAL COCCOLITOPHORIDS (EMILIANIA HUXLEYI, GEPHYROCAPSA OCEANICA, AND OCHOSPHAERA SP.) Nita Rukminasari; Muhammad Lukman; Khusnul Yaqin
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 2 (2023): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i2.7912

Abstract

The study to examine the calcification rate, adaptation, and the biotic response of three tropical coccolithophorids (Emiliania huxleyi, Gephyrocapsa oceanica, and Ochosphaera sp) to changes in CO2 concentration. Three selected calcifying coccolitophorids were grown at batch culture with CO2 system at two levels of CO2 (385 and 1000 ppm) and two light dark periods. The parameters measured and calculation including growth rate, particulate organic carbon content, particulate inorganic carbon content, chlorophyll a, cell size, photosynthetic, organic, inorganic carbon production, photosynthesis, and calcification rate.  The results showed that there was a different response to carbonate chemistry changes and dark and light periods in any of the analyzed parameters.  The growth rate of three selected calcifying microalgae tested was decreasing significantly at high concentrations of CO2 (1000 ppm) treatment on 14:10 hour light: dark periods. However, there was no significant difference between the two CO2 concentrations where they were illuminated by 24 hours light in growth rate.  The increasing CO2 concentration and light-dark periods were species-specific responses to photosynthesis and calcification rate for three selected calcifying microalgae.
Taman Epibion untuk Bioremediasi Mikroplastik secara In Situ di Perairan Makassar Achmad Nabil Al Ashad; Khusnul Yaqin; Andi Muh. Arul Efansyah; A. Nurul Izzah Malkab; Muhammad Yusran
Jurnal Pengelolaan Perairan Vol. 5 No. 1 (2023)
Publisher : Program Studi Manajemen Sumber Daya Perairan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Taman Epibion merupakan rangkaian alat yang menjadi media melekatnya organisme epibion. Epibion merupakan organisme yang melekat pada berbagai jenis substrat di perairan yang dapat menyerap mikroplastik sehingga berpotensi menjadi agen bioremediasi. Penelitian ini bertujuan untuk mengidentifikasi jenis epibion yang ditemukan pada taman epibion, menganalisis lama waktu yang dibutuhkan oleh taman epibion untuk mengumpulkan organisme epibion, mengukur jumlah konsentrasi mikroplastik dalam tubuh  epibion, serta menganalisis keefektifan taman epibion sebagai indikator dan agen bioremediasi untuk pencemaran mikroplastik. Penelitian ini dilaksanakan selama tiga bulan. Penelitian ini menggunakan metode kuantitatif berupa metode observasi dan experimental. Pengolahan data menggunakan analisis statistik deskriptif dengan uji One way ANOVA. Hasil Penelitian menunjukkan bahwa terdapat perbedaan signifikan antara rerata hitung konsentrasi mikroplastik pada sampel epibion di bulan ke-1 dan ke-2 dan tidak terdapat perbedaan signifikan antara rerata hitung konsentrasi mikroplastik pada air laut di bulan ke-1 dan ke-2. Penelitian ini memiliki kelebihan dibandingkan dengan penelitian sebelumnya karena media tumbuh epibion merupakan substrat buatan sehingga jumlah kehadiran epibion dan mikroplastik yang terkandung di dalamnya pada interval waktu tertentu dapat diukur sehingga perhitungan konsentrasi mikroplastik menjadi lebih baik.
Nutrient Absorption Rate of Oryzias celebensis Embryo Yulia Indah Sari Lalombo; Khusnul Yaqin; Sharifuddin bin Andy Omar
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.67-71

Abstract

Oryzias celebensis is a species of medaka fish in South Sulawesi. During incubation, the growth and development of these fish embryos depend on the nutrients stored in the eggs. Therefore, this study aimed to determine the volume size of egg yolks and oil droplets and the absorption rate of egg yolks of O. celebensis embryos that reared in different incubation media. The study was conducted using a completely randomized design (CRD) with four rearing media treatments, namely A (Early Rearing Media), B (bottled water), C (Pattunuang river water) and D (well water) with five replications . The results showed that the volume of egg yolk and O. celebensis embryo oil in each medium showed a decrease in volume size along with the embryonic development stage. The absorption rate of yolk in O. celebensis embryos in media A had the biggest yolk absorption with an average 0.015±0.038 mm3 while media D had the smallest yolk absorption with an average 0.011±0.022 mm3.
Microplastics contamination in green mussels Perna viridis in Pangkajene Kepulauan Waters, South Sulawesi, Indonesia Ramli; Khusnul Yaqin; Nita Rukminasari
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.1-5

Abstract

Microplastics is a problem that has been concerning, especially in marine habitat. The presence of microplastics in large quantities will have an impact on the environment and marine organisms. Marine organisms that are vulnerable to contamination by microplastics pollutants namely green mussels (Perna viridis). This study aims to determine the microplastics forms and concentration found in green mussels (Perna viridis) in Maccini Baji Waters, Labakkang District, Pangkejene Kepulauan Regency, South Sulawesi. The sampling of the mussels was carried out by the method of purposive random sampling with the sample was grouped into 3 groups of shells lengths, namely 2-3.9 cm, 4-5.9 cm and 6-7.9 cm with 33 individuals for each group. Microplastics observations were carried out using a stereo microscope. In addition to analyze microplastics concentrations, an analysis of the frequency of microplastics presence in shells was also carried out. The results showed that the microplastics was found in the form of fiber and fragments with several colors namely clear white, blue, black, red, and purple. The frequency of microplastics presence in green mussels was above 50% with an average of 71.7% exposure to microplastics. The highest frequency of microplastics presence and concentration in the range of shell lengths accounting for 2-3.9 cm.