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All Journal Jurnal Teknologi Dan Industri Pangan Media Veteriner Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Microbiology Indonesia Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kedokteran Hewan

Mirnawati Sudarwanto

Unknown Affiliation

Author-ID : 2454555

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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Peningkatan Kualitas Susu Peternakan Rakyat di Boyolali melalui Program Penyuluhan dan Pendampingan Peternak Sapi Perah Ardilasunu Wicaksono; Mirnawati Sudarwanto
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 2 No. 2 (2016): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.2.2.55-60

Boyolali District is the center area of dairy farm in Central Java Province. However, the quality of fresh milk of small scale dairy farm was still low due to a low quality of farming practice on hygiene and sanitation. The purpose of this activity was to improve the quality of fresh milk of small scale dairy farm through an extension and guidance of the farmers in using good hygiene and sanitation practices. For this purpose, 10 selecting farmers at Sruni Sub-district and 12 selecting farmers at Lanjaran Sub-district were subjected to intervention of three stages activities. First, collection of preliminary data of the level of hygiene and sanitation on milking process using Blood Agar Swab Test of milking equipment. Second, implementation of extension and advisory intervention. Third, evaluation of the microbiological milk quality based on Total Plate Count (TPC). The results showed that the hygiene and sanitation level of the first condition was considered as a bad category which 60.4% samples of fresh milk were on sixth standard (38.000 microorganism/ml of milk). After implementing extension and advisory intervention, 95.5% samples of fresh milk had a total microbiological number below SNI maximum standard (1.0 x 106 cfu/ml). Only one sample (4.5%) had TPC of 4.4 x 106 cfu/ml. Based on this result, the extension and advisory intervention could be used as a basis approach to increase the quality of fresh milk of small scale dairy farms.

Detection of Listeria monocytogenes in Pasteurized Milk Sold in Bogor and Its Relationship with Human Health AGATHA WINNY SANJAYA; MIRNAWATI SUDARWANTO; KIBUUKA ROBERT
Microbiology Indonesia Vol. 3 No. 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

Many countries have established a zero tolerance policy, under which ready-to-eat foods are contaminated with Listeria monocytogenes at a detectable level. The research was done in two parts. The first part was to qualitatively identify the presence of L. monocytogenes in pasteurized milk (n=32 samples) sold in different supermarkets in Bogor. The method was adopted from the Bacteriological Analytical Manual/Food and Drug Administration. All samples tested resulted negative to L. monocytogenes. The second part of the research was to evaluate the growth of L. monocytogenes in sterilized milk stored in an incubator set at 4oC and monitored for 7 days. The original L. monocytogenes culture at a concentration of 1x109 cfu mL-1 was diluted with buffered phosphate water 0.1% to reach a cell concentration of approximately 1.0 x 102 cfu mL-1. Growth was observed on the first, second, third, fourth and fifth day. On the sixth and seventh day, the numbers of colony forming units observed were almost similar (2.5-2.8 x 105 cfu mL-1). A population of 10 cells is sufficient to cause serious listeriosis infection in human.

AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KARI (Murraya koenigii) TERHADAP Staphylococcus aureus, Escherichia coli, dan Pseudomonas sp. Rastina R; Mirnawati Sudarwanto; Ietje Wientarsih
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui aktivitas antibakteri, konsentrasi efektif, dan pengaruh peningkatan konsentrasi ekstrak etanol daun kari (Murraya koenigii) terhadap daya hambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas sp. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri menggunakan metode difusi agar. Parameter yang diukur adalah besarnya diameter daya hambat yang terbentuk di sekitar kertas cakram. Hasil uji aktivitas antibakteri dianalisis dengan metode one way anova dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak 12,5; 25; dan 50% telah memberikan aktivitas daya hambat pertumbuhan bakteri uji. Konsentrasi efektif yang dapat menghambat bakteri Staphylococcus aureus dan Escherichia coli pada konsentrasi 50%, sedangkan bakteri Pseudomonas sp. pada konsentrasi 12,5; 25; dan 50%. Peningkatan konsentrasi ekstrak daun kari menunjukkan semakin luas diameter zona hambat pertumbuhan bakterinya. Penghambatan yang terjadi pada bakteri Staphylococcusaureus, Escherichia coli, dan Pseudomonas sp. tersebut, membuktikan bahwa daun kari mengandung senyawa aktif yang bersifat antibakteri, seperti flavonoid, fenol, alkaloid, dan saponin.

DAYA TAHAN HIDUP Toxoplasma gondii DALAM SUSU KAMBING SETELAH PASTEURISASI SUHU TINGGI WAKTU SINGKAT Rismayani Saridewi; Denny Widaya Lukman; Mirnawati Sudarwanto; Umi Cahyaningsih
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Tujuan dari penelitian adalah menetapkan daya tahan hidup takizoit Toxoplasma gondii galur RH dalam susu kambing setelah dipasteurisasi pada suhu tinggi dalam waktu singkat. Dalam penelitian ini digunakan metode in vivo dan mencit diinfeksi dengan takizoit Toxoplasma gondii galur RH secara intraperitoneal dengan konsentrasi 2,76x106 takizoit/ekor. Mencit dibagi atas tiga kelompok perlakuan, yaitu susu pasteurisasi dan takizoit yang dipanaskan pada suhu 72 °C selama 15 detik (P), susu pasteurisasi dan takizoit tanpa dipanaskan sebagai kontrol positif (KP), dan susu pasteurisasi tanpa takizoit sebagai kontrol negatif (KN). Hasil penelitian menunjukkan bahwa tidak ditemukan takizoit di dalam cairan peritoneal pada P dan KN. Takizoit Toxoplasma gondii galur RH ditemukan pada KP yang mempunyai jumlah konsentrasi hampir sama sebelum dan setelah infeksi.

Keberadaan Escherichia coli Extended Spectrum ?-lactamase Resistan Antibiotik di Peternakan Sapi Perah Cijeruk, Bogor Herwin Pisestyani; Denny Widaya Lukman; Hadri Latif; Mirnawati Sudarwanto
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.505

Contamination of Extended spectrum-betha lactamase (ESBL) producing Escherichia coli in milk can cause public health problems. The misuse of antibiotics in dairy has implications for the emergence of multi-resistant bacteria that can infect humans through food (foodborne diseases). The ESBL producing E. coli is resistant to ?-lactam antibiotics (including penicillins and 3rd and 4th derivatives of cephalosporins). This study aimed to analyze the phenotype of antibioticresistant ESBL-producing E. coli from livestock, farmer, dairy cows, milk, and pasteurized milk. The research was conducted on a dairy farm in Cijeruk District, Bogor. The method used was the ESBL EC tricycle for phenotype confirmation consisting of culture on tryptone bile x-glucuronide (TBX) agar and MacConkey agar (MCA) with the addition of the cefotaxime antibiotic 4 µg/mL for the presumption of ESBL-producing E. coli followed by confirmation of ESBL producing E. coli with indol biochemical tests with sulfite indole motility (SIM) and double disk diffusion test (DDST) on Mueller Hinton agar (MHA). The results from this study showed that ESBL producing E. coli was not found in water, effluent, milker hand swabs, teat swabs, and milk from the individual cows. ESBL producing E. coli was identified from fresh milk (2/10) and pasteurized milk (2/10) on the same farm. ESBL-producing E. coli were resistant to eight antibiotics (ampicillin, cefotaxime, ceftazidime, cefpodoxime, cephalothin, streptomycin, azithromicyn, enrofloxacin). Multidrugresistant ESBL-producing E. coli is one of the foodborne diseases that pose a health threat to the community.

Co-Authors A. Winny Sanjaya Abdulwahed Ahmed Hassan ADI SUDONO AGATHA WINNY SANJAYA Agustin Indrawati Anastasia Winny Sanjaya Andriani . Andrijanto Hauferson Angi Annytha Ina Rohi Detha Anton Apriyantono Ardilasunu Wicaksono ASEP SAEFUDDIN Betty Sri Laksmi Jenie Daowen Zhang Denny W. Lukman Denny Widaya Lukman Etih Sudarnika Ewald Usleber Feri Kusnandar Hadri Latif Harsi D. Kusumaningrum Herastuti Sri Rukmini Heri Yulianto Herwin Pisestyani I wayan Teguh Wibawan Ida Bagus Arka Idwan Sudirman IETJE WIENTARSIH Jodi Vanden Eng KIBUUKA ROBERT L.I. Sudirman Mangaraja Pidoli Tampubolon Maya Purwanti Mazdani Ulfah Daulay Ni Wajan Leestyawati palgunadi Nining Arini Nurliana . Purwanti Maya Rastina Rastina Razali . Retno Damayanti Soejoedono Rifda Naufalin Rismayani Saridewi Rita Kusriastuti Rochman Naim Srihadi Agungpriyono Surachmi Setiyaningsih Surachmi Setyaningsih Suryono . Umi Cahyaningsih Upik Kesumawati Hadi Widagdo Sri Nugroho William A. Hawley Winiati P. Rahayu

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