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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education The Indonesian Journal of Occupational Safety and Health MNJ (Malang Neurology Journal) Majalah Kesehatan FKUB Indonesian Journal of Medicine CRJIM (Clinical and Research Journal in Internal Medicine) Jurnal Klinik dan Riset Kesehatan Indonesian Journal of Biomedicine and Clinical Sciences Deka in Medicine
Didi Candradikusuma
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Co-Authors Abellia, Gaby Agustina Tri Endharti Al-Rasyid, Harun Amalia, Dinda Antariksa, Genta Asmiragani, Syaifullah Badrul Munir, Badrul Bagus Hermansyah Burhan, Niniek Chozin, Iin Noor Dewi Indiastari Dwi Budi Santoso Dwi, Pratiwi Suryanti Dwikarlina, Intan Eko Arisetijono Erma Sulistyaningsih Evatta, Arde Fathoni, Alfan Fatma, Endah Gatoet Ismanoe Hendrik Wahyudi Heri Sutanto Heri, Sutanto Hidayat Sujuti Indiastari, Dewi Kristyana, Septian Loeki Enggar Fitri Machlusil Husna, Machlusil Milanitalia Gadys Rosandy Natasia, Septalisa Niniek Budiarti Niniek Budiarti B Nur Samsu Purbasari, Bethasiwi Rendy Wijaya Rosandy, Milanitalia Rosandy, Milanitalia Gadys Samsul Arifin Santoso, Dimas Shahdevi Nandar Kurniawan, Shahdevi Nandar Sinta Silvia Sri Budhi Rianawati, Sri Budhi Tri Wahju Astuti, Tri Wahju Tuda, Josef Sem Berth Umar Zein Widodo Mardi Santoso, Widodo Mardi Winoto, Eden Suryoiman Yudha, Nyoman Satvika Dharma Yulia Rusmayanti, Mega