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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences AJIE (Asian Journal of Innovation and Entrepreneurship) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Akuatik: Jurnal Sumberdaya Perairan Jurnal Perikanan Universitas Gadjah Mada COJ (Coastal and Ocean Journal)
Asadatun Abdullah
Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Bogor 16680 Telepon (0251) 8622915 Faks. (0251) 8622916
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Social Sciences

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Optimization of Genetic Material Extraction Techniques and Application of Isothermal Amplification Method for Field Authentication of Two Thresher Sharks (Alopias pelagicus and Alopias superciliosus) Aldina, Vatia Dwi Rizki Ramadhani; Abdullah, Asadatun; Rianti, Puji; Jacoeb, Agoes Mardiono
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.147-154

Abstract

The pelagic thresher shark (Alopias pelagicus) and bigeye thresher shark (Alopias superciliosus) are important shark species for Indonesia's consumption and finning industry. Both Alopias species are included in the CITES appendix II, thus requiring certain documents for trading. Regarding species identification for on-site application, the DNA isolation method is a crucial step. In this study, we developed a DNA isolation method suitable for on-site application based on isothermal amplification (LAMP) and species-specific COI gene markers. Three different extraction methods were applied, namely modified spin column kits and dipsticks. The quality of DNA was evaluated and tested for isothermal amplification using a reference sample, fresh fillet, and ethanol-preserved sample. The extracted sample concentration was in the range of 135.35-0.65 ng/μL. The LAMP test showed that three different DNA extraction methods successfully amplified the DNA fragments through the color changes at the end point of the LAMP reaction. The LAMP test was also sufficient to detect less than 10 ng of DNA from A. pelagicus and A. superciliosus within 30-50 min. The DNA from the modified spin column and dipstick extraction method combined with LAMP can potentially be used to detect Alopias pelagicus and Alopias superciliosus species on-site.
Co-Authors . Ernawati . Nurjanah . Nurjanah Ade Irma Suryani Nasution Agoes M. Jacoeb Agoes M.Jacoeb Agoes Mardiono Jacoeb Aldina, Vatia Dwi Rizki Ramadhani Anggrei Viona Seulalae Aninditya Artina Setiaputri Anita Nurul Firdaos Apriandi, Azwin Ari Elisa Ratih Aulia Azka Aulia Sekar Sari Bustami Ibrahim Chairun Nufus Chairun Nufus Chandabalo Chandabalo EVI MAYA SARI Fatimah Abdillah Giri Rohmad Barokah Hana Aulia Sativa Hanifah Husein Hizbullah Ikromatun Nafsiyah Irama Dramawanti Pamungkas Joko Ahadi Priyanto Kinanti Permata Sitaresmi Laili Izzati LINAWATI HARDJITO M. Fedi A. Sondita Mala Nurilmala Misla Alfitri Muhamad Fedi Alfiadi Sondita Muhammad Reyhan N. Nurjanah N. Nurjanah Nadia Fabella Nanang Kurnia Naufal Muharam Nurdin Norita Norita Novia Nanda Saputri Nur Azizah Nasution Nurjanah Nurjanah Nurjanah Nurjanah - Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah nurjanah Pipih Suptijah Puji Rianti Rachmawati Rusydi Rahma Dini Arbajayanti Ramlan Raudhi Kurniawan Rizky Chairunisah Rizsa Mustika Pertiwi Rizsa Mustika Pertiwi Rizsa Pertiwi Rodieser Sembiring Roni Nugraha Roza Yusfiandayani Ruddy Suwandi Sahaba, Muh. Alsere Bardian Seftylia Diachanty Shabrina Aulia Sharah Novita Sari Shindy Hamidah Manteu Siti Fauziyah Siti Nur Hakimah TATI NURHAYATI Taufik Hidayat Vicentius Marco Matutina