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Perbandingan Metode Isolasi DNA pada Produk Perikanan Segar dan Olahan: Comparison of DNA Isolation Methods for Fresh and Processed Seafood Aninditya Artina Setiaputri; Giri Rohmad Barokah; Muh. Alsere Bardian Sahaba; Rahma Dini Arbajayanti; Nadia Fabella; Rizsa Mustika Pertiwi; Mala Nurilmala; Roni Nugraha; Asadatun Abdullah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i3.32314

Seafood product is an important commodity in the global trade market, however, seafood fraud has been increasingly reported. Therefore, a method to specifically identify species of seafood products is needed. DNA methods gain many interests for detection of seafood fraud due to its robustness. This study was aimed to determine the effectiveness of several methods for isolation of DNA from fresh and processed seafood. The DNA isolation methods used in this study were the CTAB DNAzol, TianGen, Qiagen, and Fasmac.. DNA concentration and purity were determinedusing nanodrop spectrofotometry and electrophoresis. DNA isolation showed that the DNA concentration of fresh seafood products was higher than the processed seafood products. DNA isolation using CTAB method have higher levels of DNA purity (1.60-2.00) than using commercial kits in all samples. The commercial kits offer a simple procedure and minimize the possibility of environmental contaminants.

Komponen Asam Amino dan Aktivitas Enzim Tripsin dari Usus Tuna Sirip Kuning (Thunnus albacares) dan Kakap Merah (Lutjanus campechanus): Amino Acid Components and Trypsin Enzyme Activity from Intestines of Yellowfin Tuna (Thunnus albacares, Bonnaterre 1788) and Red Snapper (Lutjanus campechanus, Poey 1860) Rahma Dini Arbajayanti; Tati Nurhayati; Mala Nurilmala
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 24 No 1 (2021): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v24i1.33878

Jeroan ikan dikenal sebagai salah satu sumber enzim pencernaan yang melimpah, terutama sebagai sumber proteinase pencernaan yang baik, salah satunya adalah tripsin. Jenis dan habitat ikan akan memengaruhi sifat tripsin ikan. Tujuan penelitian ini adalah untuk membandingkan komponen asam amino daging dan aktivitas ekstrak kasar enzim tripsin dan usus ikan tuna sirip kuning (Thunnus albacares) dan ikan kakap merah (Lutjanus campechanus). Kedua jenis ikan diamati morfometrik dan rasio bobot jeroan ikan. Selain itu komposisi kimia, komponen asam amino daging, dan aktivitas ekstrak kasar enzim tripsin dari usus kedua jenis ikan tersebut kemudian dianalisis... Secara umum ikan tuna sirip kuning memiliki komposisi asam amino yang lebih tinggi dibandingkan ikan kakap merah, terutama pada komponen asam amino histidina, leusina, asam aspartat, lisina dan asam glutamat. Komponen asam amino yang paling dominan terdapat pada kedua jenis ikan tersebut adalah asam glutamat. Enzim tripsin ekstrak kasar usus ikan tuna memiliki aktivitas yang lebih tinggi yaitu 4,908 U/mg dibandingkan dengan ekstrak kasar usus ikan kakap merah sebesar 0,076 U/mg.

The Combination of Medium and Method of Cultivation on The Growth Performance of Porphyridium cruentum Hasanah, Hasanah; wulandari, Wulandari; afriani, Afriani; Hariski, M.; Muji Rahayu, Dyah; Arbajayanti, Rahma Dini
Sriwijaya Journal of Environment Vol 10, No 1 (2025): Environmental Impacts
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Porphyridium cruentum has advantages in pigments and exopolysaccharides, so it has the potential to be developed in the food, pharmaceutical, nutraceutical, and cosmetic industries. Porphyridium cruentum cultivation media uses synthetic and indoors, causing high costs. This study aims to determine the growth performance of Porphyridium cruentum in different mediums and method of cultivation. The research methods used cultivating Porphyridium cruentum for 7 days with four treatments, namely indoors Guillard media (IGM), outdoors Guillard media (OGM), indoors fertilizer media (IFM), and outdoors fertilizer media (OFM. Each treatment had three replications. Growth was identified using an optical density spectrophotometer uv-vis, gravimetrically dried weight of biomass and pH adjustment during 7 days of cultivation. The result shows the best growth performance in IFM, followed by IGM, OFM, and OGM. Fertilizer media is better for the growth of Porphyridium cruentum than Guillard media. pH growth Porphyridium cruentum during cultivation 7-7.5. Indoor cultivation is better than outdoor cultivation. The highest biomass was IFM, which had the best performance in IFM with OD 0.247, pH 7, and biomass 79.6%.

DISTRIBUSI VERTIKAL DAN HORIZONTAL SUHU DAN SALINITAS DI TAMAN NASIONAL PERAIRAN NATUNA SAAT MUSIM BARAT TAHUN 2008 (STUDI KASUS: PULAU LAUT) Lestariningsih , Wiwid Andriyani; Khaldun, Muhammad Hafidz Ibnu; Arbajayanti, Rahma Dini
Mantis Journal of Fisheries Vol. 1 No. 02 (2024): Desember 2024
Publisher : Department of Fisheries, Animal Science Faculty, Universitas Jambi.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/mjf.v1i02.40058

Taman Nasional Perairan Natuna merupakan salah satu kawasan konservasi laut yang dilindungi karena memiliki keanekaragaman hayati yang sangat penting dalam menjaga keseimbangan ekosistem laut serta mendukung keberlanjutan sumber daya alam perairan Indonesia. Pengelolaan kawasan konservasi memerlukan berbagai pertimbangan dan pemahaman mengenai kondisi fisik seperti adalah parameter suhu dan salinitas. Penelitian ini bertujuan untuk memberikan gambaran yang lebih komprehensif mengenai distribusi suhu dan salinitas di TNPN, khususnya di Pulau Laut. Data pada penelitian ini menggunakan data Argo Float dan akan dianalisis secara spasial dan vertikal dengan analisis stratifikasi perairan. Distribusi horizontal nilai suhu berkisar 25.99-27.31°C dan salinitas berkisar antara 33.01-33.31‰. Distribusi vertikal suhu berkisar antara 25.5-26.5°C dan 33.25-33.4‰. pada awal musim Barat terdapat lapisan homogen pada kedalaman 0-5 m dan bervariasi didaerah pesisir. Variasi nilai suhu dan salinitas diduga karena percampuran vertikal yang diinduksi oleh angin musim barat yang bergerak dengan kecepatan rata-rata 6.69 m/s. Sehingga, Taman Nasional Perairan Natuna cocok untuk dikembangkan sebagai kawasan konservasi.

The Combination of Medium and Method of Cultivation on The Growth Performance of Porphyridium cruentum Hasanah, Hasanah; wulandari, Wulandari; afriani, Afriani; Hariski, M.; Muji Rahayu, Dyah; Arbajayanti, Rahma Dini
Sriwijaya Journal of Environment Vol 10, No 1 (2025): Environmental Impacts
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22135/sje.2025.10.1.11-16

Porphyridium cruentum has advantages in pigments and exopolysaccharides, so it has the potential to be developed in the food, pharmaceutical, nutraceutical, and cosmetic industries. Porphyridium cruentum cultivation media uses synthetic and indoors, causing high costs. This study aims to determine the growth performance of Porphyridium cruentum in different mediums and method of cultivation. The research methods used cultivating Porphyridium cruentum for 7 days with four treatments, namely indoors Guillard media (IGM), outdoors Guillard media (OGM), indoors fertilizer media (IFM), and outdoors fertilizer media (OFM. Each treatment had three replications. Growth was identified using an optical density spectrophotometer uv-vis, gravimetrically dried weight of biomass and pH adjustment during 7 days of cultivation. The result shows the best growth performance in IFM, followed by IGM, OFM, and OGM. Fertilizer media is better for the growth of Porphyridium cruentum than Guillard media. pH growth Porphyridium cruentum during cultivation 7-7.5. Indoor cultivation is better than outdoor cultivation. The highest biomass was IFM, which had the best performance in IFM with OD 0.247, pH 7, and biomass 79.6%.

Study on the Spawning Techniques of Jelawat Fish (Leptobarbus hoevenii) at Balai Perikanan Budidaya Air Tawar (BPBAT) Sungai Gelam Anggraini, Gira; Arbajayanti, Rahma Dini; Rolin, Febrina; Pangentasari, Dwinda; Leni, Yusyam; Hafidz Ibnu Khaldun, Muhammad; Putinur, Putinur; Roiska, Riris; Bareta, Ainun Rohmawati; Hasanah, Hasanah
Mantis Journal of Fisheries Vol. 2 No. 03 (2025): Desember 2025
Publisher : Department of Fisheries, Animal Science Faculty, Universitas Jambi.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/mjf.v2i03.50236

This study aims to determine the spawning techniques of Leptobarbus hoevenii fish at the Sungai Gelam Freshwater Aquaculture Center (BPBAT). Activities include maintaining prospective broodstock, selecting mature broodstock, injecting ovulation hormones, and the spawning process, stripping, egg handling, water quality monitoring to highlight its importance, and embryo development. Ovaprim hormone injections were administered to the female broodstock four times and once to the male broodstock to stimulate ovulation. The stripping process was carried out after ovulation and was followed by dry mixing of eggs and sperm before being spread into the hatching tank. The results showed a fertilization rate of 63,08% and a hatching rate of 9,73%, which were influenced by gamete quality and the physiological condition of the broodstock. Observations of embryogenesis showed developmental stages ranging from morula, blastula, gastrula, to organogenesis before the eggs hatch into larvae. Water quality parameters during the study were in the optimal range for spawning, namely pH 7,24–7,77, temperature 27–30,5°C, and DO 3,53–5,58 mg/L. The results of this study explain that the success of Hoven’s carp fish seeding is highly dependent on the readiness of the broodstock, the application of artificial spawning techniques, and good water quality management.

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