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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Teknologi Pangan dan Gizi Jurnal Penelitian Pascapanen Pertanian MIX : Jurnal Ilmiah Manajemen Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Kedokteran Hewan Current Biomedicine

Trioso Purnawarman

Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Institut Pertanian Bogor

Author-ID : 2480235

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Keberadaan Bakteri Listeria monocytogenes pada Keju Gouda Produksi Lokal dan Impor (PRESENCE OF LISTERIA MONOCYTOGENES IN LOCAL AND IMPORTED GOUDA CHEESES) Debby Fadhilah Pazra; Trioso Purnawarman; Denny Widaya Lukman
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.719 KB)

Listeria monocytogenes is included in the foodborne pathogen, which has been associated with severaloutbreaks of human listeriosis especially in high risk groups. Listeria monocytogenes could be found inGouda cheeses because of poor hygienic and sanitation practices. In addition, this bacteria could surviveduring the making of cheese and cheese ripening process. The purpose of this study was to identify thepresence of L. monocytogenes in local and imported Gouda cheeses and how the safety level of the Goudacheese against contamination of L. monocytogenes. This study used the conventional method in accordancewith the Bacteriological Analytical Manual, US Food and Drug Administration and Bergey’s Manual ofDeterminative Bacteriology to detect the presence of L. monocytogenes at 15 samples of local Gouda cheeseand 15 samples of imported Gouda cheese sold in supermarkets in Jakarta and Bogor. The results of thisstudy showed that was not found L. monocytogenes in local and imported Gouda cheese. It could be concludedthat is Gouda cheese relatively safe from L. monocytogenes and meets Indonesian National Standard.

Identifikasi Escherichia coli yang Resistan Antibiotik pada Daging Burger yang Dijual di Sekitar Kampus IPB Dramaga Bogor Kumala Andri Asari; Denny Widaya Lukman; Trioso Purnawarman
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mengetahui cemaran Escherichia coli dan resistansinya terhadap antibiotik pada sampel daging burger yang dijual pada radius 2 km batas Kampus Institut Pertanian Bogor di Dramaga, Bogor. Sebanyak 35 sampel daging burger diperoleh dari semua pedagang burger (tujuh pedagang). Isolasi dan identifikasi E. coli mengacu pada Standar Nasional Indonesia 2897:2008. Uji resistansi terhadap antibiotik menggunakan metode Kirby-Bauer dengan acuan dari Clinical Laboratory Standards Institute tahun 2018. Uji resistansi antibiotik dilakukan terhadap semua isolat E. coli yang didapat dari semua koloni E. coli yang diisolasi dari sampel daging burger. Antibiotik yang digunakan dalam penelitian ini adalah ampisilin, amoksisilin, streptomisin, oksitetrasiklin, tetrasiklin, sefotaksim, gentamisin, enrofloksasin, asam nalidiksat, kloramfenikol, dan trimetoprim-sulfametoksasol. Data dianalisis secara deskriptif. Hasil pengujian menunjukkan11,4% (4 dari 35 sampel) positif E. coli. Isolat yang diperoleh dari empat sampel ada lima isolat E. coli (100%) yang resistan terhadap antibiotik yang diuji. Resistansi tertinggi ditemukan pada antibiotik streptomisin (100%) dan gentamisin (80%). Dari lima isolat E. coli yang didapat, empat isolat di antaranya telah resistan terhadap tiga atau lebih golongan antibiotik yang dikenal sebagai multi-drug resistant (MDR), dengan pola resistansi yang terdiri atas AMP-AMC-S-CN (ampisilin-amoksisilin-streptomisin-gentamisin), AMP-S-ENR-NA-SXT-CN (ampisilin-streptomisin-enrofloksasin-asam nalidiksat-trimetoprim sulfametoksasol-gentamisin), dan AMP-S-ENR-NA-SXT-OT-CN (ampisilin-streptomisin- enrofloksasin-asam nalidiksat- trimetoprim sulfametoksasol-oksitetrasikilin-gentamisin). Keberadaan E. coli resistan pada daging burger yang termasuk pangan siap santap (ready-to-eat food) dapat menimbulkan risiko kesehatan pada konsumen.

Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Daging Ayam pada Pasar Tradisional di Kota Bogor (ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM CHICKEN MEAT OF TRADITIONAL MARKETS IN THE CITY OF BOGOR) Connie Januari; Mirnawati Bachrum Sudarwanto; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic use in farm is spread widely to treat of poultry disease including therapy, supportive or preventive use and as afeed additive to improve chicken performance. The negative effects of antibiotic use can increase the level of bacterial resistance to antibiotics. This study aimed to investigate on antibiotic resistance in Escherichia coli isolated from chicken meat that were sold in Traditional Market of Bogor City. A total of 175 samples of chicken meat were taken by purposive sampling method, out of 175 found 50 positive samples of E. coli. The samples were subjected to E. coli examination and the isolated E. coli were tested for the antibiotic resistance using eight antibiotics, i.e., amoxicillin, cefotaxime, colistin, nalidixid acid, streptomycin, erythromycin, oxytetracillin, and tetracycline. The study was conducted by using the disk diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute guidelines. The study showed E. coli isolated from chicken meat were resistance towards amoxicilin (90%), colistin (94%), nalidixid acid (86%), streptomycin (98%), erythromycin (98%), oxytetracillin (84%), tetracycline (86%), and cefotaxime antibiotics (12%). The proportion of multidrugresistant was 99%. The higher of multidrug-resistant indicated the E. coli would be a threat to public and environmental health.

Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Trioso Purnawarman; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Agus Setiyono; Muharam Saepulloh
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.261 KB)

Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.

Resistensi Antibiotik Terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat (ANTIBIOTIC RESISTANCE TO ESCHERICHIA. COLI ISOLATED FROM BROILER FARMS IN SUBANG DISTRICT, WEST JAVA PROVINCE) Aji Barbora Niasono; Hadri Latif; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic resistance in avian bacterial pathogens is a common problem in the Indonesia poultry industry. The present study aimed to provide information on the present status of antibiotic resistance in Escherichia coli (E. coli) from a broiler farm environment. The research method uses a cross sectional study with a stratified random sampling pattern. E. coli was collected from litter using boot swab method at the broiler house farms in Subang District. E. coli isolates (n = 74) were obtained from 74 samples of boot swabs. The sample was isolated and identified morphologically and biochemically using the IMVIC test to obtain E. coli isolates. The test was followed by a sensitivity test for nine antibiotics, namely tetracycline, sulfamethoxazole, trimethoprim, ampicillin, nalidixic acid, ciprofloxacin, enrofloxacin, gentamicin and chloramphenicol by using the agar dilution method. The interpretation of the results of this sensitivity test refers to the Clinical and Laboratory Standards Institute CLSI VET01S (2015) and CLSI M100 (2018). Measurement of the level of knowledge is done using a structured questionnaire that has been tested for validity and reliability. E. coli was isolated from 74 samples of boot swab. 93.2% of E. coli samples were found to be MDR with the highest prevalence in four types of antibiotics at 40.5%. The most common resistances observed were against tetracycline 97.3%, sulfamethoxazole 87.8%, trimethoprim 74.3%, ampicillin 68.9%, nalidixic acid 64.8%, ciprofloxacin 45.9%, enrofloxacin 40.5%, gentamicin 28.4% and chloramphenicol 10.8%. The findings of this study revealed the high level of antibiotic resistance in broiler environment is worrisome and have negative implications for human and animal health.

Co-Authors A.S. Satyaningtijas Afiff , Usamah Agus Setiyono Agustin Indrawati Aji Barbora Niasono Amalo, Gabryella Fransina Anisa Rahma, Anisa annisa, rizka fajri Aulia Andi Mustika Ayunina, Yasmine Qurrota Chairun Nisa Connie Januari Debby Fadhilah Pazra Denny Widaya Lukman Dwi Desmiyeni Putri Dwida Agustina Suherman Engki Zelpina Etih Sudarnika Fachriyan Hasmi Pasaribu Gabryella Fransina Amalo Hadiyanti, Cresensia Rara Hadri Latif Hasudungan A Sidabalok Hasudungan A Sidabalok, Hasudungan A Herwin Pisestyani I wayan Teguh Wibawan IETJE WIENTARSIH Joko Pamungkas Karunia Maghfiroh Khoirun Nisa Kumala Andri Asari Lina Noviyanti Sutardi Madyastuti, Rini Maulita Lutfiani Melani Wahyu Adiningsih Mohamad Syamsul Ma’arif Monika Danaparamitha Andriani Muharam Saepulloh Nugroho, Radhitya Aryo Okti Nadia Poetri Rahmat Setya Adji Retno Damayanti Soejoedono Rusman Efendi Santosa, Alfian Sa’diah, Siti Septa Walyani Sudarwanto, Mirnawati Bachrum Surachmi Setiyaningsih Susanto Nugroho Syafril Daulay Syahdu Pramono Tarigan, Ronald Vindriati, Zukhrufa Vista Yasmine Qurrota Ayunina

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