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Journal : Jurnal Veteriner

Respons Imun Puyuh (Coturnix coturnix Japonica) Dewasa yang Mendapat Ekstrak Daun Singkong dalam Mengatasi Dampak Cekaman Panas (IMMUNE RESPONSE OF ADULT QUAILS (COTURNIX COTURNIX JAPONICA) TREATED WITH CASSAVA LEAF EXTRACT TO OVERCOME HEAT STRESS) Koekoeh Santoso; Anindita Sista Widyadhari; Okti Nadia Poetri; La Jumadin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.42 KB) | DOI: 10.19087/jveteriner.2019.20.4.519

Abstract

The aim of the research was to prove the potency of cassava leaf extract to overcome heat stress of adult quails on the variable of antibody titer of Newcastle Disease (ND), MDA level, and total protein. The research was divided into four groups and conducted in 6 replications for each group, consisting of control group, group A, B, and C. All the groups were exposed to heat stress, and then treated with cassava leaf extract with different dosages for 5,292 mg/168 g body weight, 10,584 mg/168 g body weight, and 21,168 mg/168 g body weight for 28 days after being adapted for a week. Variables of antibody titer of Newcastle Disease (ND), MDA level, and total protein were measured every week. The result showed that HI titer of overall tested groups was less than 2 HI units. HI titer contains positive antibody of antibody titer of ND if it reaches 16 HI units. HI test in the present study showed that all of quail serums contained negative antibody of ND. The level of MDA fluctuated with the highest and smallest value was found in group B and control group, respectively. In addition, the administration of cassava leaves extract tended to decrease total protein, where control group was significantly different to both group A and C (P < 0,05). In conclusion, cassava leaves extract has the potential to decrease the stress level, but the administration up to 21,168 mg/168 g BB has not been able to decrease the level of MDA in quails that suffered from heat stress. In Dosage 21, 168 mg extract of cassava leaves was potential to lower stress level so that it was unable to stimulate immune respond to form ND titer antibody.
Profil Leukosit Puyuh (Coturnix coturnix japonica) yang Mendapat Ekstrak Daun Singkong dalam Mengatasi Dampak Cekaman Panas Koekoeh Santoso; Anindita Sista Widyadhari; Okti Nadia Poetri; La Jumadin
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.141 KB)

Abstract

Penelitian ini bertujuan mengkaji potensi daun singkong (Manihot esculenta) dalam mengatasi cekaman panas pada puyuh terhadap parameter total leukosit, diferensiasi leukosit dan indeks stres. Penelitian ini dibagi menjadi empat kelompok. Kelompok kontrol serta Kelompok A, B, dan C masing-masing mendapat cekaman panas, kemudian diberi ekstrak klorofil daun singkong 5,292, 10,584, dan 21,168 mg/ 168 g bobot badan per oral selama 28 hari setelah diadaptasikan satu minggu. Parameter seperti total leukosit, diferensiasi leukosit dan indeks stres dilakukan setiap minggu. Hasil penelitian menunjukkan bahwa menunjukkan jumlah leukosit mengalami penurunan yang tidak berbeda nyata sejalan dengan peningkatan dosis ekstrak. Limfosit kelompok perlakuan ekstrak daun singkong (A, B, dan C) lebih tinggi (P < 0,05) dibandingkan kontrol, sedangkan heterofil kelompok perlakuan ekstrak daun singkong (A, B, dan C) lebih rendah (P < 0,05) dibandingkan kontrol. Monosit dan eosinofil kelompok perlakuan ekstrak daun singkong (A, B, dan C) cenderung lebih rendah dibandingkan kontrol. Pengamatan basofil menunjukkan hasil yang tidak berbeda nyata (P < 0,05) antara kontrol dengan kelompok perlakuan ekstrak daun singkong. Hasil penelitian lain menunjukkan bahwa semakin tinggi nilai H/L maka semakin tinggi tingkat stres hewan. Rasio H/L tertinggi terlihat pada kelompok kontrol, yang diikuti dengan kelompok perlakuan A, B, dan C. Penurunan tingkat stres puyuh teramati sejalan dengan peningkatan dosis ekstrak daun singkong yang diberikan. Simpulan pada penelitian ini adalah pemberian ekstrak daun singkong mampu menurunkan total leukosit serta rasio H/L. Selain itu, pemberian ekstrak daun singkong cenderung menurunkan monosit, heterofil, dan eosinofil serta meningkatkan limfosit.
Respons Kekebalan Tubuh Ayam IPB D1 terhadap Infeksi Virus Penyakit Tetelo (Newcastle Disease) (IMMUNE RESPONSE OF IPB D1 CHICKEN AGAINST NEWCASTLE DISEASE VIRUS ) Retno Setyaningsih; Sri Murtini; Okti Nadia Poetri; Cece Sumantri
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.58 KB)

Abstract

Newcastle disease (ND) or Tetelo is an endemic poultry disease in Indonesia, and caused significant economic losses. Instead of diseases control programme has been carried out, ND outbreaks still occurs among poultry flock. IPB D1 chicken is a type of cross-breeding chickens between pelung-sentul crossbred chicken male and kampung-broiler crossbred chicken female, this chicken is genetically selected based on on body resistance to disease. The aim of our research is to study immune response of IPB D1 against ND infection. Fourty IPB D1 chickens were used in this study, and divided into three groups : vaccinated group, unvaccinated group, control group. Vaccinated group received two time ND vaccination at age 10 weeks and booster at age 12 weeks. Unvaccinated and control group were remain not vaccinated. At age 13 weeks, vaccinated and unvaccinated group were challenge with ND virus (107 EID50/ml). Immune response were determined based on antibody titer which is measured by haemagglutination inhibition (HI) test and the profile of white blood cell of the chicken. Our result showed that both vaccinated and unvaccinated group has ND antibody titer e” log 24 at challenge time, and survived after challenge. White blood cell profile among all groups showed that lymphocites has the higher number than other leukocyte type. It can be concluded that IPB D1 chickens showed a good response against ND virus.
Berisiko Tinggi Titik Masuk Hewan Pembawa Rabies di Kabupaten Sumbawa dan Dompu, Provinsi Nusa Tenggara Barat Nurjumaatun Nurjumaatun; Eko Sugeng Pribadi; Okti Nadia Poetri
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.380

Abstract

Rabies is a zoonosis caused by infection of Lyssavirus belong to Rhabdoviridae family. Dompu and Sumbawa District, which are located on Sumbawa Island, West Nusa Tenggara Province, were designated as rabies outbreak in 2019. This study was aim to qualitatively estimate the magnitude of the risk of rabies-carrying animals (RCA) entry at each entry point for RCA to Sumbawa and Dompu Regencies, NTB Province. The risk assessment was carried out following risk analysis guidelines of the United NationsWorld Organization forAnimal Health (World Organization for Animal Health, Office Internationale de Epizootic, OIE), and referring to Australian Biosecurity methods. The uncertainty assessment refers to the European Food Safety Authority (EFSA). The results showed that the entry points were in Lunyuk sub-District, Sumbawa sub-District, Labuhan Badas sub-District, Moyo Hilir sub-District, Moyo Hulu sub-District, Unter Iwes sub-District in Sumbawa District, and Pekat sub-District, Kempo sub-District, Manggelewa sub-District, Hu’u subDistrict and Woja sub-Districts in Dompu District. Based on the research results, it can be concluded that rabies-carrying animals entry points in NTB Province are classified as high risk. The high risk at rabies-carrying animals entry points is due to the lack of public awareness and knowledge about rabies.
Laporan Kasus: Studi Morfopatologi Demam Babi Afrika pada Babi di Bogor, Jawa Barat Rahayu Woro Wiranti; Dwi Utari Rahmiati; Aldo Yanuar Wuriyantara; Okti Nadia Poetri; Ekowati Handharyani
Jurnal Veteriner Vol 24 No 4 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.4.564

Abstract

Demam Babi Afrika atau African swine fever (ASF) adalah penyakit menular yang dapat terjadi pada ternak babi dan babi liar. Penyakit tersebut disebabkan oleh virus ASF. Gejala klinis sangat bervariasi dari kasus yang terjadi perakut, akut, subakut dan kronis tergantung pada virulensi virus. Kejadian ASF dapat menyebabkan angka kematian tinggi pada babi, penurunan kondisi sosial, dan ekonomi termasuk kontaminasi lingkungan. Berdasar beberapa pertimbangan masalah tersebut, maka akan sangat bermakna bila dilakukan studi morfopatologi dan klarifikasi terhadap kejadian ASF pada babi (Sus scrofa domesticus). Kajian saat ini dilaksanakan dengan melakukan prosedur nekropsi terhadap dua ekor babi umur empat bulan, yaitu dengan melakukan pemeriksaan kondisi tubuh dan organ-organ interna. Sampel organ diambil untuk pemeriksaan histopatologi yang selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan lanjut dilakukan dengan metode polymerase chain reaction (PCR) menggunakan primer universal (p72 dan p54). Hasil pemeriksaan makroskopik menunjukkan bahwa beberapa bagian kulit mengalami erythema, terjadi splenitis hemoragi, enteritis hemoragi disertai dengan lymphadenopathy pada limfonodus mesenterika dan terjadi hemoragi pada meningen dan pembuluh darah di otak. Hasil pemeriksaan histopatologimenunjukkan lesi pada organ limforetikuler seperti limpa dan limfonodus mesenterika, yaitu radang limfositik disertai hemoragi. Pemeriksaan pada otak menunjukkan lesi radang limfositik disertai hemoragi pada selaput meningen. Hasil pemeriksaan molekuler dengan metode PCR memberikan konfirmasi bahwa penyakit disebabkan oleh virus ASF. Berdasarkan hasil pemeriksaan komprehensif secara makroskopik, mikroskopik dan evaluasi PCR dapat disimpulkan bahwa kedua ekor babi tersebut menderita infeksi ASF tipe akut.