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EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT Usma Aulia; Sri Wahyuni; Gholib Gholib; Dasrul Dasrul; Mulyadi Adam; Erdiansyah Rahmi; Abdullah Hamzah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.432 KB) | DOI: 10.21157/j.ked.hewan.v17i1.30042

Abstract

The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.
PEMBERDAYAAN KELOMPOK TERNAK MELALUI BUDIDAYA ITIK PETELUR DI GAMPONG TANJONG SELAMAT KABUPATEN ACEH BESAR, PROVINSI ACEH Hamny Sofyan; Taufan Hidayat; Sri Wahyuni; Mulyadi Adam; Amalia Sutriana; Zainabun Zainabun
Jurnal Media Tropika Vol 1 No 2 (2021): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v1i2.5467

Abstract

Tanjong Selamat is one of the villages directly adjacent to Universitas Syiah Kuala, Banda Aceh. Its strategic location makes Tanjong Selamat Village a priority to be developed into an assisted village of Universitas Syiah Kuala. The main occupations of local Tanjong Selamat villagers are farmers and breeders. Efforts to increase the community income can be done through the cultivation of laying ducks and post-harvest management in the form of making salted eggs. This community service activity was carried out in the form of extension, demonstration, and training. The activity materials are knowledge about the prospects for laying ducks cultivation, prevention and treatment of diseases that often attack ducks, good cage systems, management of breeding, and the making of salted eggs. The cage system and breeding management were carried out by making a pilot cage consisting of the main cage of 15 m2 and a breeding area of 50 m2. The cage was filled with 50 domestic ducks (peking ducks and tegal ducks) aged 4 months. During the community service activity, the participants were very enthusiastic and able to implement the technology package that had been given. Based on the activity that has been conducted, the villagers of Tanjong Selamat Village, especially the members of the livestock group, can understand well the extension material and have high enthusiasm in improving the management of laying ducks as observed during the demonstration of the cage plot as well as better understand the making of salted eggs that are in accordance with consumer desires and have a long shelf life.
The Assessment of Animal Welfare Implementation in Poultry Slaughter House in Kisaran City Asahan District Ridho Akbar Yuandi; Mulyadi Adam; Sri Wahyuni
Jurnal Medika Veterinaria Vol 16, No 1 (2022): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v16i1.13395

Abstract

This study aimed to assess the aspect of animal welfare in poultry slaughter house at Bakti market, Kartini market, and Diponegoro market in Kisaran city Asahan District North Sumatra Province. This research was conducted using direct observation and interview methods using a structured questionnaire based on animal welfare aspects. There were three aspects assesed in this study, including transportation aspects, shelter aspects and slaughter aspects. The results of the studies showed that some of the animal welfare aspects have been applied positively in poultry slaughter house in Kisaran City. However, improvements are required to be implemented in some poultry slaughter house in this area
Study of Cat’s Welfare Kept at Pet Shops in Bekasi West Java Reza Sofa Hartuti; Mulyadi Adam; Triva Murtina
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i1.3333

Abstract

The aim of this study was to find out correlation between cat’s welfare and caring management in a number of cats kept at p etshops inBekasi from January to February 2013. This study used a questionnaire with ratings based on five aspects of animal welfare (feeding, healthy,comfortable, fear, and normal behavior) and through direct observation. Data was analyzed using Correlation Test Chi Square with 95%confidence interval. The result showed that there is the corelation between animal welfare and caring management of cats at pet shop in Bekasi,West Java.____________________________________________________________________________________________________________________Key words: animal welfare, cats, pet shops 
11. Study of Cat’s Welfare Kept at Pet Shops in Gajah Mada Mal Central Jakarta Putri Aisyah Hanifah; Mulyadi Adam; Roslizawaty Roslizawaty; Abdullah Hamzah; Erdiansyah Rahmi; T. Armansyah TR
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3743

Abstract

The aim of this study was to find out correlation between cat’s welfare and caring management in a number of cats kept at pet shops in Gajah Mada mal Central Jakarta, from January to February 2016. This study used a questionnaire with ratings based on five aspects of animal welfare (freedom from hunger and thirst, freedom from discomfort, freedom from pain, injury, and disease,  freedom from fear and distress, and freedom to express normal behavior) and through direct observation. Data was analyzed by Correlation Test Chi Square with 95% confidence interval. The result shows that there is a significant correlation (P0,05) in freedom from hunger and thirst with freedom from fear and distress aspects, and there is no correlation (P0,05) between caring management and animal welfare standart in freedom from pain, injury, and disease aspects,  freedom from discomfort aspect, and freedom to express normal behavior with caring management aspects.
Perubahan Morfologi dan Morfometri Saluran Reproduksi Kelinci Lokal Bunting Semu Hasil Induksi Menggunakan GnRH Sri Wahyuni; Syafruddin Syafruddin; Muhammad Fathan Rizky Athallah; Tongku Nizwan Siregar; Mulyadi Adam; Roslizawaty Roslizawaty
Jurnal Agripet Vol 23, No 1 (2023): Volume 23, No. 1, April 2023
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v23i1.25377

Abstract

ABSTRACT. Bunting semu pada kelinci dapat terjadi karena adanya induksi secara hormonal dan stimulasi fisik yang dapat menyebabkan perubahan pada morfologi saluran reproduksi. Salah satu metode induksi bunting semu adalah melalui injeksi GnRH. Penelitian ini bertujuan mengidentifikasi perubahan terhadap anatomi dan histologi saluran reproduksi pada kelinci lokal bunting semu yang diinduksi dengan penyuntikan GnRH. Dalam penelitian ini digunakan sembilan ekor kelinci betina lokal, berumur 1-2 tahun dengan bobot badan 1,8-2,2 kg. Kelinci yang dibagi ke dalam tiga kelompok perlakuan (n=3) yakni K1, K2, dan K3. Kelompok K1 (kontrol negatif), diinjeksi dengan 0,1 ml NaCl fisiologis tanpa perkawinan; K2 (kontrol positif), kelinci diinduksi dengan 100 IU PMSG, tiga hari kemudian dikawinkan dan diinjeksi 75 IU hCG; dan K3, kelinci diinduksi dengan penyuntikan 5 g GnRH secara intravena. Pada hari ke-8 setelah perlakuan seluruh kelinci disembelih lalu saluran reproduksi diambil untuk pengamatan morfologi dan morfometri oviduk, kornua uteri, serviks uteri dan vagina lalu diproses secara histoteknik dan diwarnai dengan pewarnaan hematoksilin dan eosin untuk pengamatan histologi dan histomorfometri. Panjang oviduk, kornua, dan vagina kelinci memperlihatkan perbedaan yang nyata (P0,05) antar K1 dan K2 dengan K3. Ketebalan lapisan muskularis ampula, lamina propia isthmus, lamina muskularis isthmus, tunika serosa isthmus memperlihatkan perbedaan yang nyata (P0,05) antara K1 dan K2 dengan K3. Tebal lapisan endometrium kornua uterus K2 berbeda sangat nyata (P0,05) dengan K3, namun tidak berbeda nyata (P0,05) dibandingkan K1. Lapisan serosa serviks uteri K1 dan K2 berbeda sangat nyata (P0,05) dengan K3. Histomorfometri vagina kelinci menunjukkan perbedaan yang nyata (P0,05). Berdasarkan hasil penelitian dapat disimpulkan bahwa induksi GnRH tidak menyebabkan perubahan morfologi dan morfometri saluran reproduksi kelinci lokal pada hari ke-8 setelah induksi.(Morphology and morphometry changes of reproductive tract in the pseudo pregnant doesusing GnRH)ABSTRAK. Pseudopregnancy in rabbits can occur due to hormonal induction and physical stimulation that can cause changes in the morphology of the reproductive tract. One method of hormonal induction is GnRH injection. This study aimed to determine the anatomical and histological changes of the reproductive tract in pseudo pregnant local rabbits induced by injection of GnRH. In this study, nine local female rabbits, aged 1-2 years with a body weight of 1.8-2.2 kg, were divided into three treatment groups (n=3), namely, K1 (negative control): injected with 0.1 ml of physiological NaCl without mating, K2 (positive control): injected with 100 IU PMSG and then mated with male rabbits after three days of injection and followed by injection of 75 IU Hcg; K3 injection 5g of GnRH intravena route. On the day-8 after treatment, all rabbits were slaughtered and the reproductive tract was collected for morphological and morphometric observation of the oviduct, uterine horn, uterine cervix, and vagina, and then processed histotechnically and stained with hematoxylin and eosin for histological and histomorphometric observation. The length of oviduct, uterine horn and vagina showed significant different (P0.05) between K1 and K2 with K3. The thickness of the lamina muscularis of ampulla, lamina propia of isthmus, lamina muscularis of isthmus, tunica serous of isthmus showed significant different (P0.05) between K1 and K2 with K3.The thickness of the endometrium of uterine horn was significant different (P0.05) between K2 and K3, but not significantly different (P0.05) with K1. The thickness of tunica serous of servical uterine K1 and K2 was significant different (P0.05) with K3. Vaginal histomorphometry of rabbits was not significantly different (P0.05). It can be concluded that the induction of GnRH did not changes the morphology and histology of the reproductive tract in the local rabbits on the day-8 after treatment.
Efektivitas Pemberian Beberapa Preparat Hormon Prostaglandin Komersial terhadap Persentase Berahi Sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara Novika Ayuni Rambe; Tongku Nizwan Siregar; Teuku Armansyah TR; Gholib Gholib; Budianto Panjaitan; Mulyadi Adam; Dasrul Dasrul
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16317

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh perbedaan PGF2 analog terhadap persentase berahi sapi di Labuhanbatu Selatan. Sampel yang digunakan dalam penelitian ini adalah data hasil UPSUS SIWAB tahun 2017. Dalam penelitian ini digunakan data 2.547 ekor sapi yang memiliki variasi breed dan umur, dengan skor kondisi tubuh baik. Sapi-sapi dikelompokkan menjadi tiga kelompok berdasarkan preparat sinkronisasi berahi yang digunakan. K1= kelompok sapi yang diinjeksi dengan 5 ml PGF2-1 (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%) berjumlah 1.300 ekor. K2= kelompok sapi yang diinjeksi dengan 5 ml PGF2-2 (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml) berjumlah 600 ekor. K3= kelompok sapi yang diinjeksi dengan 2 ml PGF2-3 (cloprostenol 75 mg/ml dan chlorocresol 1,0 mg/ml) berjumlah 647 ekor. Penyuntikan dilakukan secara intramuskulus, dua kali dengan interval 10 hari. Data yang diperoleh kemudian dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Withney. Hasil analisis menunjukkan persentase berahi pada kelompok 1; 2; dan 3 masing-masing 80,7%; 50% dan 61,8% (P0,01). Dari hasil penelitian dapat disimpulkan bahwa PGF2-1 mempunyai efektivitas lebih baik dibandingkan PGF2-2 dan PGF2-3 dalam induksi sinkronisasi berahi pada sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara.(Effectiveness of different commercial prostaglandin hormone administration on the percentage of estrous in cattles in South Labuhanbatu, North Sumatera)ABSTRACT. The aim of this study was to determine the effect of differences PGF2 analogues on the percentage of estrus in cattles in South Labuhanbatu. The sample used in this study was the 2017 SIWAB UPSUS data. Data of 2,547 cattles with various breed and age as well as in good body condition scores were selected. The cattles were grouped into three data groups based on the hormone used for estrus synchronization. The cattles in data group I (1300 cows) were injected with 5 ml PGF2-1 (dinoprost tromethamine 5 mg/ml and benzyl alcohol 1.65%). The cattles in data group II (600 cows) were injected with 5 ml PGF2-2 (5.5 mg/ml dinoprost tromethamine and 12.0 mg/ml benzyl alcohol). The cattles in data group III (647) were injected with 2 ml of PGF2-3 (cloprostenol 75 g/ml and chlorocresol 1.0 mg/ml). Injections were carried out intramuscularly, twice at 10-day intervals. The data obtained were then analyzed Kruskal Wallis and continued with Mann Withney test. The results showed that the percentage of estrus in groups I; II; and III were 80.7%; 50% and 61.8% (P0.01). From the results of the study it can be concluded that Lutalyse has better effectiveness than Capriglandin and Sincrovall in the induction of synchronization estrus in cattle in South Labuhanbatu, North Sumatera.
Persentase Kebuntingan Kambing Betina Resipien Setelah Pelaksanaan Embrio Transfer Ginta Riady; Mulyadi Adam; Idawati Nasution
Jurnal Agripet Vol 2, No 1 (2001): Volume 2, No. 1, April 2001
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v2i1.3087

Abstract

ABSTRACT. A study on the pregnanncy rate of recipient does following embryio transfer procedure had been carried out. This study used 2 donor does and 10 recipient does. Donor does are subjected to both estrous synchronization (using progesterone in CIDR-G device) and superovulation protocols using follcle stimulating hormone in Folltropin-V. However, recipient does are only subjected for estrous synchronization protocol. Estroussynchronization protocol ni donor does and recepientb ones are conducted at the same tame. Embryo collection is carried out through cervix at day 7 following breeding. Parameter of this study is pregenancy rate of recipient does fillowing embryo transfer. Data collected in this study will be analyzed descriptively. Recipient does received two embryos by non-surgery method. This study showed that recipient does were foun non-pregnant during pregnancy diagnosis. It can be related to the incidence of early embryonic death are nutrion and overcrowding in uterus. It can be conclude that embryo transfer procedure carried out in this study was not effective.