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Journal : International Journal of Renewable Energy Development

Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System Damayanti, Astrilia; Sarto, Sarto; Sediawan, Wahyudi Budi
International Journal of Renewable Energy Development Vol 9, No 1 (2020): February 2020
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.9.1.37-42

Abstract

Biohydrogen production via dark fermentation is a prospective renewable energy technology. In the process, reused of immobilized mixed culture is very important as their activities greatly influencehydrogen production. The aim of this work was to evaluate the reuse of alginate beads affecting the biohydrogen production for 45 days. This study in batch reactor were performed using glucose 10 M as substrate, alginate and activated carbon as immobilization matrix materials, chicken eggshell as buffer, and cow dung biodigester as mixed culture. Hydrogen and pH on fermentation product are investigated by gas chromatography (GC) technique and pH meter, respectively. The colony diameter on immobilized and co-immobilized mixed culture was observed using optical microscope and colony diameter was measured using Image-Pro Plus Software v4.5.0.29. The surface morphology of immobilization and co-immobilization beads were determined using scanning electron microscope (SEM). The results showed that the colonies growth observed using optical microscopy or SEM was apparent only in the immobilization of mixed culture. The average growth and diameter of colonies per day were 90 colonies and 0.025 mm, respectively. The weight of beads and pH during the 45-day fermentation process for bead immobilization of mixed culture were 1.32–1.95 g and 6.25–6.62, correspondingly, meanwhile, the co-immobilizations of the mixed culture were 1.735–2.21g and 6.25–6.61, respectively. In addition, the average hydrogen volume of glucose fermented using an eggshell buffer and reusing the immobilization and co-immobilization beads was 18.91 mL for 15 cycles.©2020. CBIORE-IJRED. All rights reserved
Comparative Study on the Various Hydrolysis and Fermentation Methods of Chlorella vulgaris Biomass for the Production of Bioethanol Megawati, Megawati; Bahlawan, Zuhriyan Ash Shiddieqy; Damayanti, Astrilia; Putri, Radenrara Dewi Artanti; Triwibowo, Bayu; Prasetiawan, Haniif
International Journal of Renewable Energy Development Vol 11, No 2 (2022): May 2022
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2022.41696

Abstract

One of the microalgae that can be potentially used to produce bioethanol is Chlorella vulgaris, as it is rich in carbohydrates. However, the carbohydrates in C. vulgaris cannot be converted directly into ethanol. This study aimed to investigate the chemical and enzymatic hydrolysis of C. vulgaris, which is subsequently followed by fermentation. The catalysts used in the chemical hydrolysis were hydrochloric acid, sodium hydroxide, and potassium hydroxide, while the enzymes used were the mixture of alpha-amylase + glucoamylase, alpha-amylase + cellulase, and alpha-amylase + glucoamylase + cellulase. The hydrolysate obtained from chemical hydrolysis was fermented through Separate Hydrolysis Fermentation (SHF), while the one from enzymatic hydrolysis was fermented through Simultaneous Saccharification and Fermentation (SSF), in which both processes used S. cerevisiae. After undergoing five hours of enzymatic hydrolysis (using alpha-amylase + glucoamylase), the maximum glucose concentration obtained was 9.24 ± 0.240 g/L or yield of 81.39%.  At the same time and conditions of the substrate on chemical hydrolysis, glucose concentration was obtained up to 9.23 + 0.218 g/L with a yield of 73.39% using 1 M hydrochloric acid. These results indicate that chemical hydrolysis is less effective compared to enzymatic hydrolysis. Furthermore, after 48 hours of fermentation, the ethanol produced from SHF and SSF fermentation methods were 4.42 and 4.67 g/L, respectively, implying that producing bioethanol using the SSF is more effective than the SHF method.
Co-Authors Ababil, Devara Candra Abdurrafi, Faishol Ahmed Tessario Ekanuramanta Amelia Fitri Ananda Muthi Athirah Angga Pratama Anggun Septiamurti Ar Rasyid, Moh. Rizal Artanti Putri, Dewi Ayasha Maharani Ayu Nur Permadhini Bahlawan, Zuhriyan Ash Shiddieqy Bariroh, Siti Bayu Triwibowo Catur Rini Widyastuti, Catur Rini Desy Hikmatul Siami Dewi Selvia Fardhyanti Doni Rahmat Wicakso Eko Setyawan Endah Ayu Fitriana Eva Amalia Alvionita Eva Amalia Alvionita Fadlurrohman Fadlurrohman Fahreza Pracenda Felicitia Fitriana, Endah Ayu Fitriyani, Winda Risma Fortuna, Dwi Hanif Ardhiansyah Hanif Ardhiansyah Haniif Prasetiawan Harianingsih, Harianingsih Hernadin, Ivan Aldino Hutomo , Gayuh Aditya Hutomo, Gayuh Aditya Isaroyati, Luluk Karenina Hakim Kevin Thomas Khoridatus Sulwa Krisdayanti , Shendy kurniawan, Adhetya Larasati, Amalia Layliyah, Marifatul Luluk Arvi Cahyaning Suwandi Luqman Buchori M. Burhan Rubai Wijaya Maftuchan, Maftuchan Magfiroh, Meilina Maharani, Anggun Megawati Megawati - Megawati Megawati Megawati Megawati Megawati Megawati Megawati Megawati Melanie Hartalia Putrie Miftahuddin Azhari Moh. Rizal Ar Rasyid Noor Ubay, Isnina Nur Kholifah Chandra Mulyani Nur Kholifah Chandra Mulyani Nurkhasanah, Eva Nurul Huda Nurul Khusna Nuryoto Nuryoto Nuryoto Oktaviana, Vira Prasetyo, Ridwan Anung Prayogo, Robby Danang Qurnaini, Rika Alda Radenrara Dewi Artanti Putri Radenrara Dewi Artanti Putri, Radenrara Dewi Artanti Radenrara Putri Rahimsyah Ijas Nur Rasyid Rahmayetty Rahmayetty Revasha Ajeng Kamalia Reyhan Dwi Saputra Riana Defi Mahadji Putri Rizki, Muhammad Prabu Salma Alfahra Choirunisa Salwa, Dian Fatimatus Sandra Anggita Fadriana Sarto Sarto - Sarto Serlia Fitri Sadan Siti Bariroh Sulardjaka, S Syafitri, Laila Taufan Satya Wiguna Triani, Fita Tsani Muftidar Wahyudi Budi Sediawan Wahyudi Budi Sediawan Waryanto, Arif Wibowo, Muhammad Yusuf Widaya, Adi Risma Widi Astuti Widi Astuti Widi Widayat Wijaya, Muhammad Burhan Rubai Winaningsih, Ima Yusuf Rumbino Zulfi Fitriani