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All Journal MEDIA MEDIKA INDONESIANA Jurnal Berkala Epidemiologi Jurnal NERS The Indonesian Biomedical Journal Jurnal Abdi Insani JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Asian Journal of Health Research Archives of Pediatric Gastroenterology, Hepatology, and Nutrition Folia Medica Indonesiana
Andy Darma
Department Of Child Health Faculty Of Medicine, Universitas Airlangga/ Dr. Soetomo General Academic Hospital, Surabaya, East Java, Indonesia
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Journal : The Indonesian Biomedical Journal

 1 
Zinc Administration Affects Bronchial Mucosal NF-κB p105/p50, p-NF-κB p65, IL-8, and IL-1β of Zinc-deficient Rats Reza Gunadi Ranuh; Andy Darma; Alpha Fardah Athiyyah; Ergia Latifolia; Retno Asih Setyoningrum; Boerhan Hidajat; Siti Nurul Hidayati; Anang Endaryanto; Ferry Sandra; Subijanto Marto Sudarmo
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.1041

Abstract

BACKGROUND: Risk of acute respiratory infections in children less than 5 years of age is up to 95%. Zinc deficiency is one of the main risk factors. This study aimed to explore the effect of zinc on the bronchial mucosae inflammatory status expressed by nuclear factor (NF)-κB p105/p50, NF-κB p65, interleukin (IL)-8, and IL-1β.METHODS: Twenty-four Wistar rats were divided into 4 groups: normal zinc diet group without zinc supplementation (Z1), normal zinc diet group with zinc supplementation (Z2), zinc deficient diet group without zinc supplementation (Z3), and zinc deficient diet group with zinc supplementation (Z4). NF-κB p105/p50, p-NF-κB p65, IL-8, and IL-1β were measured by immunohistochemical staining.RESULTS: The inflammatory status of bronchial mucosae between Z1 and Z2 groups showed no difference (p=0.055). However, the inflammatory status of bronchial mucosae between Z3 and Z4 groups showed significant difference (p<0.01). Multivariate factorial design showed that zinc supplementation was beneficial when given to zinc deficient diet group with regard to decrease p-NF-κB p65, IL-8 and IL-1β levels (p<0.001) and increase dendritic cell (p=0.022).CONCLUSION: Zinc administration under conditions of zinc deficiency affects the inflammatory status, as shown by the decrease of p-NF-κB p65, IL-8 and IL-1β and the increase of NF-κB p105/p50.KEYWORDS: zinc, NF-κB, p105/p50, p65, IL-8, IL-1β, rat
Lactobacillus plantarum IS-20506 Probiotic Restores Galectin-4 and Myosin-1a Expressions in Duodenum, Jejunum and Ileum of Lipopolysaccharide-induced Rats Reza Gunadi Ranuh; Alpha Fardah Athiyyah; Andy Darma; Wibi Riawan; Ingrid Suryanti Surono; Ferry Sandra; Subijanto Marto Sudarmo
The Indonesian Biomedical Journal Vol 12, No 3 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i3.1098

Abstract

BACKGROUND: Galectin-4 and Myosin-1a are important proteins for normal intestinal brush border structure and composition. Damage of these proteins by inflammation may alter digestion, absorption and barrier function. Probiotic has been widely known in maintaining gut health. However, the molecular mechanism of Lactobacillus plantarum IS-2056 probiotic in repairing intestinal brush border is not well defined. Therefore, current study was conducted by investigating the Galectin-4 and Myosin-1a expressions in a rodent model.METHODS: Male Wistar rats were induced with/without lipopolysaccharide (LPS) and treated with/without L. plantarum IS-2056 probiotic. On the seventh day, duodenum, jejunum, and ileum were collected and analyzed with western blot and immunohistochemistry for Galectin-4 and Myosin-1a expressions.RESULTS: Rats administrated with L. plantarum IS-2056 probiotic showed significant increase of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum compared to the control group (p<0.05). While in control group, Galectin-4 level tended to increase in more distal of intestinal segment and Myosin-1a level tended to decrease in more distal intestinal segment.CONCLUSION: L. plantarum IS-20506 probiotic may facilitate the repairment of damaged intestinal brush border as demonstrated by significant restoration of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum of LPS-induced rats.KEYWORDS: Lactobacillus plantarum, IS-20506, probiotic, galectin-4, myosin-1a, duodenum, jejunum, ileum
Zinc Supplementation Effect on the Bronchial Cilia Length, the Number of Cilia, and the Number of Intact Bronchial Cell in Zinc Deficiency Rats Andy Darma; Alpha Fardah Athiyyah; Reza Gunadi Ranuh; Wiweka Merbawani; Retno Asih Setyoningrum; Boerhan Hidajat; Siti Nurul Hidayati; Anang Endaryanto; Subijanto Marto Sudarmo
The Indonesian Biomedical Journal Vol 12, No 1 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i1.998

Abstract

BACKGROUND: Airway epithelium is the first line of defense against a variety of exposures. Inflammatory processes, hyperresponsiveness and zinc deficiency cause epithelial damage. Zinc is involved in apoptosis and microtubule formation. However, its role in the integrity of bronchial mucosa and cilia is unclear.METHODS: To assess the effect of zinc on the integrity of the bronchial epithelium, 24 male Rattus norvegicus strain Wistar rats were randomized into four experimental groups: normal zinc diet group without zinc supplementation, normal zinc diet group with 60 ppm zinc supplementation, zinc deficient diet group without zinc supplementation, and zinc deficient diet group with 120 ppm zinc supplementation. Bronchial mucosal integrity was measured with the number of epithelial cells, and the number and length of cilia.RESULTS: Number of cell in normal zinc diet group was 8.8±1.82, while it was only 8.1±1.08 in zinc deficient diet group (p<0.001). Number of cilia per cell was 4.6±1.08 in normal zinc diet group, compared to 4.0±0.79 in zinc deficient diet group (p<0.001). Ciliary length also differ by 7.68±0.66 μm in normal zinc diet group and only 5.16±0.91 μm in zinc deficient diet group (p<0.001).CONCLUSION: Zinc supplementation of the normal zinc diet group affected the length of bronchial cilia. Zinc supplementation of the zinc deficient diet group affected the integrity of the bronchial epithelium, which was shown by the number and length of cilia, and the number of epithelial cells.KEYWORDS: zinc, bronchial epithelial integrity, cilia length, number of cilia, epithelial cell 
Co-Authors Alpha Fardah Athiyyah Ana Bilah, Aisah Nur Anak Agung Putri Nadia Paramitha Anang Endaryanto Ariani Setiawati Aries Budianto Arya Satya Rajanagara Bambang Pudjo Semedi BUDIYANTO Burhan Mahendra Kusuma Dadik Rahardjo, Dadik Dadik Raharjo Dimas Setyanto Dwiyanti Puspitasari, Dwiyanti Eko Budi Koendhori, Eko Budi Ergia Latifolia Fahrizal Rizky Muharram Ferry Sandra Fitria, Anisa Lailatul Hidajat, Boerhan I Gusti Made Reza Gunadi Ranuh Ingrid Suryanti Surono Juniastuti Juniastuti Kamal Musthofa Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Laura Navika Yamani Maharani, Aussie Tahta Mira Irmawati Mohammad Hud Suhargono Muhammad Faizi Muhammad Faizi, Muhammad Muhammad Irawan Muhammad Shoifi Muhammad Tidar Abiyu Muhammad Yusuf Nesa, Ni Nyoman Metriani Ni Nyoman Metriani Nesa Ni Nyoman Metriani Nesa Novi Rahayu Arianti PA, Deanty Ayu PA, Deanty Ayu Rachmat Agung Widodo Rahardian Indarto Susilo Raharjo, Dadik Retno Asih Setyoningrum Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Ranuh Reza Ranuh Romdhoni, Achmad Chusnu S.Pd. M Kes I Ketut Sudiana . Sacharissa Zerlina Tsarwah Thirafi Shirakawa, Toshiro Shirakawa, Toshiro Siti Nur Faidah SITI NURUL HIDAYATI Sofia Wardhani Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto steven christian susianto Steven Christian Susianto Subijanto MS Subijanto Subijanto Sudarmo, Subijanto Marto Sumitro, Khadijah Rizky Susianto, Steven Christian Sutrisno Sutrisno Toshiro Shirakawa Vina Lidya Setjaputra Wibi Riawan Wiweka Merbawani Zayyin Dinana