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All Journal Folia Medica Indonesiana Journal of Health (JoH) Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat JPM (Jurnal Pemberdayaan Masyarakat) Jurnal Analis Kesehatan Jurnal Kesehatan Medical Technology and Public Health Journal Medica Hospitalia Medicra (Journal of Medical Laboratory Science/Technology) Academia Open Humanism : Jurnal Pengabdian Masyarakat Medical Scope Journal (MSJ) Jurnal Abdimas Kartika Wijayakusuma Indonesian Journal of Innovation Studies Procedia of Social Sciences and Humanities Journal of Noncommunicable Diseases (JOND) Prosiding University Research Colloquium Indonesian Journal Health Science and Medicine
Andika Aliviameita
Universitas Muhammadiyah Sidoarjo
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Variations in Erythrocyte Count and Hemoglobin Levels in Blood Samples Collected Using Conventional Anticoagulants and Vacutainers with Different Secondary Homogenization Techniques Batis, Afifah; Aliviameita, Andika
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 9 No 2 (2025): Medical Technology and Public Health Journal September 2025
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v9i2.7006

Abstract

The choice of anticoagulants and the methods of homogenization are critical pre-analytical steps that can significantly influence the accuracy of laboratory results. This study aims to assess the differences in erythrocyte count and hemoglobin levels among blood samples processed with conventional anticoagulants and Vacutainer tubes, taking into account variations in secondary homogenization techniques. A laboratory experimental design was employed, utilizing 32 blood samples acquired from eight participants. The analysis was conducted using an automated hematology analyzer. Data were subjected to a Paired T-Test, indicating no significant differences in erythrocyte counts (p=0.617) and hemoglobin levels (p=0.510) between conventional EDTA samples that were homogenized four times and those homogenized eight times. However, a significant difference was noted in erythrocyte counts (p=0.018), while hemoglobin levels remained unaffected (p=0.393) in EDTA vacutainer samples subjected to four versus eight rounds of secondary homogenization. Additionally, a significant difference was found in both erythrocyte counts (p = 0.008) and hemoglobin levels (p < 0.0001) between conventional EDTA samples and those subjected to four rounds of secondary homogenization using vacutainers. Similarly, there was a significant difference in erythrocyte counts (p = 0.006) and hemoglobin levels (p = 0.014) between conventional EDTA samples and Vacutainer samples that underwent eight rounds of homogenization.  Keywords: Blood Specimen Collection; EDTA; Erythrocyte; Hemoglobin; Secondary Homogenization
Co-Authors Ahmad Shobrun Jamil Ahmad Yudianto Ainutajriani, Ainutajriani Alda Megawati Amilya, Widya Intan Anggie Wedha Puspita ARDIANSYAH, SYAHRUL Arief Wisaksono Ariyanti, Silvia Asri, Nur Indah Nila Aziza, Vidayatul Batis, Afifah Diah Ariana Dian Delta Dina Trikumalasari Dira Nadila Ellies Tunjung Sari Maulidiyanti, Ellies Tunjung Sari Emilia, Hilda Evi Rinata Fadilatin, Lisa Anis Firmansyah, Nukayo Fitrian Desi Prameswari Hadie, Djauharoh A Intantri, Risma Jamilatur Rohmah Krisdianti, Rika Lestari, Dzulia Livia Nur Azzah Meryn Linanda Agustin Mieke Sylvia Miftahul Mushlih Millah, Hamidah Wardatul Murosidah, Pratasya Liyaajul Mushlih , Miftahul Nevy Elensya NURUL AZIZAH p Puspitasari, p Primastuti, Yuridistya Putri Puspita, Anggun Puspitasari Puspitasari Puspitasari Puspitasari Qilmia Fanani Ramadhan, Syahrul Ibnu Mubaroq Riantika, Mayang Rini, Chylen S. Rini, Chylen Setyo Rohman Dijaya Saidah, Sukma Nur Salim, Siti Zaenab Savitra, Igfina El Segara, Bayu Setiyo Rini, Chylen Silvia Ariyanti Siska Aprilia Siti Zaenab Salim Sri Mukhodim Faridah Hanum Wahyuni, Nining Xena, Alfanny Juliannindy Yanik Purwanti Yasmin, Rizka Afifah Yonika Zakaria, Rizal