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Journal : Jurnal Kedokteran Hewan

THE EFFECT OF BLACK CUMIN (Nigella sativa) SUPPLEMENTATION THROUGH DRINKING WATER ON THE HISTOLOGY OF SMALL INTESTINE AND LARGE INTESTINE OF BROILER CHICKENS Madi Hartono; Purnama Edy Santosa; Ratna Ermawati; Muhammad Mirandy Pratama Sirat
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.882 KB) | DOI: 10.21157/j.ked.hewan.v15i3.19774

Abstract

This study aimed to determine the effect of black cumin (Nigella sativa) supplementation through drinking water on the histology of broiler chickens’ small intestine and large intestine. The research was conducted from April-September 2020 in a cage facility of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. This research used a completely randomized design with four treatment groups and three replications (five broilers per replication) with a total of 60 male broilers. The treatments were drinking water without Black Cumin (P0, control); drinking water with Black Cumin 36 mg/kg BW/day (P1); 72 mg/kg BW/day (P2); and 144 mg/kg BW/day (P3). Three broilers from each group were randomly necropsied at 31 days old, and samples of the small intestine (duodenum, jejunum, and ileum) and large intestine were fixed with 10% formalin solution and sent to the Lampung Disease Investigation Center for histological preparations. The observation of preparations was carried out microscopically using the Leica DM500® Binocular Microscope to accurately calculate various parameter sizes. The results were analyzed statistically with one way Analysis of Variance at significant level 5% and if proven significant, then a Tukey test was conducted. The results of this study were that the supplementation of black cumin 72 mg/kg BW/day through drinking water could significantly increase (P0.05) 1) the average sizes of villi height and villi area of small intestine (duodenum, jejunum, ileum) and large intestine (colon) of broiler chickens; 2) the average sizes of basal villi width, villi apex width, and gland diameter of broiler duodenum; and 3) the average size of apex villi width of broiler colon. The conclusion of this study was the supplementation of black cumin at dose of 72 mg/kg BW/day through drinking water could increase the histological sizes of the small intestine and large intestine of broilers.
THE EFFECT OF BLACK CUMIN (Nigella sativa) SUPPLEMENTATION THROUGH DRINKING WATER ON THE HISTOLOGY OF SMALL INTESTINE AND LARGE INTESTINE OF BROILER CHICKENS Hartono, Madi; Santosa, Purnama Edy; Ermawati, Ratna; Sirat, Muhammad Mirandy Pratama
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i3.19774

Abstract

This study aimed to determine the effect of black cumin (Nigella sativa) supplementation through drinking water on the histology of broiler chickens small intestine and large intestine. The research was conducted from April-September 2020 in a cage facility of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. This research used a completely randomized design with four treatment groups and three replications (five broilers per replication) with a total of 60 male broilers. The treatments were drinking water without Black Cumin (P0, control); drinking water with Black Cumin 36 mg/kg BW/day (P1); 72 mg/kg BW/day (P2); and 144 mg/kg BW/day (P3). Three broilers from each group were randomly necropsied at 31 days old, and samples of the small intestine (duodenum, jejunum, and ileum) and large intestine were fixed with 10% formalin solution and sent to the Lampung Disease Investigation Center for histological preparations. The observation of preparations was carried out microscopically using the Leica DM500 Binocular Microscope to accurately calculate various parameter sizes. The results were analyzed statistically with one way Analysis of Variance at significant level 5% and if proven significant, then a Tukey test was conducted. The results of this study were that the supplementation of black cumin 72 mg/kg BW/day through drinking water could significantly increase (P0.05) 1) the average sizes of villi height and villi area of small intestine (duodenum, jejunum, ileum) and large intestine (colon) of broiler chickens; 2) the average sizes of basal villi width, villi apex width, and gland diameter of broiler duodenum; and 3) the average size of apex villi width of broiler colon. The conclusion of this study was the supplementation of black cumin at dose of 72 mg/kg BW/day through drinking water could increase the histological sizes of the small intestine and large intestine of broilers.
Co-Authors Abimanyu Prastyo Ardiansyah Adek Rayhan Regisa Aksal Alfarizki Alfarizki, Aksal Alfaruq, Fahmi Fadhilah Ali Husni Arif Qisthon Aris Haryanto Ayu Fitriani Ayu Lidyana Ayu Lidyana Bayu Hadi Setya Irawan Budiyanto, Ferly Rahmat Charles Rangga Tabbu Cici Hardiyanti Dakhlan, Akhmad Deny Sapto Chondro Utomo Desmawan, Deris Dhani, Akbar Rahmat Dian Septinova Dini Wahyu Yudianingtyas Emi Maimunah Erwanto Erwanto Erwanto Fajar Shodiq Permata Fani Setiawan Farida Fathul Fatmawati, Siti Tika Fauzi, Teo Achmad Fina Arzakiyah Fitria Tsani Farda Fitria Tsani Farda Guntur Januar Yudhistira Hanafi, Riyan Hanip Rangga Saputra Hardiyanti, Cici Hari, Putu Gaura Hermilinda Parera Hizkia, Ezra I Kadek Dwi Agus Candra Wijaya Ilhamsyah, Alifudin Imam Widodo Imam Widodo Juandita, Karina Natasya Karina Natasya Juandita Kusuma, Yosea Talenta Lidyana, Ayu Liman Liman Liman Liman Liman, Liman Madi Hartono Madi Hartono Madi Hartono Madi Hartono Madi Hartono Mahfud Rivai Medania Purwaningrum Mia Widowati Michael Haryadi Wibowo Muhammad Mirandy Pratama Sirat Muhammad Mirandy Pratama Sirat Muhiddin Sirat Muhtarudin Muhtarudin mulya, arif eka Nisak, Rohmatin Nur Aini Nur Aini Pramana, Sandika Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Rais, Ambarwati Mirna Ramadanti, Andin Hairunnisa Rivai, Mahfud Riyan Hanafi Riyan Hanafi Rohmatin Nisak Rr Riyanti, Rr S Siswanto Safira, Auliya Sagala, Yollanda Natalia Sandika Pramana Sani, Darwan Sanjaya, Purba Setio, Slamet Shaleh, Shofyan Sherlina Widya Rahma Sherlina Widya Rahma Sirat, Muhiddin Siswanto Siswanto Siswanto Siswanto Siswanto Siswanto Siti Tika Fatmawati Siti Tika Fatmawati Slamet Setio Sri Suharyati Surmini Surmini Surmini Surmini Surmini, Surmini Syahrio Tantalo Syahrio Tantalo Syahrio Tantalo Teo Achmad Fauzan Teo Achmad Fauzi Teo Achmad Fauzi Utami Syifana Widyastuti Veronica Wanniatie Widowati, Mia Yollanda Natalia Sagala Yudhistira, Guntur Januar Yuliana, Darma Zulvina Afrianti