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All Journal Bioeducation Journal BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan BIO-SITE |BIOLOGI Sains Terapan Jurnal Pendidikan Fisika Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Basicedu International Journal of Educational Dynamics (IJEDS) Atrium Pendidikan Biologi BiosciED: Journal of Biological Science and Education TROPICAL GENETICS International Journal of Humanities Education and Social Sciences Tropical Genetics Jurnal Serambi Biologi Biodik: Jurnal Ilmiah Pendidikan Biologi
Yuni Ahda
Universitas Negeri Padang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Journal : TROPICAL GENETICS

 1 
Primer design, in silico PCR and optimum annealing temperature for Escherichia coli detection in refillable drinking water samples Afifatul Achyar; Annisa Irna Putri; Dwi Hilda Putri; Yuni Ahda
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1092.036 KB)

Abstract

Refill Drinking Water Depots (DAMIU) in the community are easy to find at affordable prices, which is a concern in the feasibility of refill drinking water quality. E.coli is one of the pathogenic bacteria present in drinking water that has poor quality. When it enters the body it can cause symptoms of diarrhea, fever, vomiting and others. The purpose of this study was to create a specific primer for E.coli that can be used to detect E.coli and determine the optimum temperature of primer annealing. Researchers do a new primer design because the existing design does not necessarily produce the same results due to various factors of different experimental conditions. The primer design was carried out on an in silico-based and had to meet the criteria for a good primer because it would be used in vitro. E.coli gene sequences was aligned with Shigella sp. using Pairwise Alignment. Primer candidates were analyzed using NCBI's Primer3 and Geneious Prime tools. The result is that the first primer pair is forward 5'–ATGCAGTGGTTCCTTATCTCACA-3' reverse 5'- ATCCTTAATGGCACTGCGCT-3', amplifying the amplicons along 417 bp in the yraJ gene. The second primer pair, forward 5'-CAGAACGTTTTTCATTCAGCAGG-3' reverse 5'-GCCACTACCAGATCGAGTCA-3' amplifies the 573 bp amplicon in the rne gene. The optimum annealing temperature for both pairs of primers was 59.5oC.
Dermatoglyphic Analysis of Asthma Sufferers in a Population Of Students At The Faculty of Mathematics and Science, Universitas Negeri Padang Velina Salsabil; Afifatul Achyar; Yuni Ahda
Tropical Genetics Vol. 2 No. 2 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i2.30

Abstract

Asthma is a disease defined as chronic inflammation of the airways which is characterized by recurrent coughing and shortness of breath. Dermatoglyphs are the appearance of tendrils and tendril patterns on the fingers, palms, toes and soles. Dermatoglyph is used as an aid in the detection of a number of diseases that have a strong heritable basis and is also used to detect abnormalities. This study aims to analyze and compare the patterns of dermatoglyphics in asthma sufferers and non-asthmatics on the fingertips. This type of research is descriptive research with cross sectional sampling method. Samples were taken from the student population of the Faculty of Mathematics and Natural Sciences, Padang State University as many as 100 samples of fingerprint patterns. The results obtained showed that there was a significant difference in the number of arch fingerprint patterns of asthmatics compared to normal individuals. Seeing this significant difference in numbers indicates a possible link between fingerprint patterns and asthma phenotypes.
Co-Authors Abdul Razak Achyar, Afifatul Ahmad Saleh Ali Amran Ali Imran Annisa Irna Putri Atifah, Yusni Berry Devanda Chakra Wirangga Dinelti Fitria Dwi Hilda Putri Dwi Junita Zega Eldyana Rahayu Putri Febri Yanti Festiyed Festiyed Fitri Arsih Fitria, Yanti Heffi Alberida Helendra . Husnin Nahry Yarza Indra Hartanto Kiki Mulyani Lufri Lufri Mades Fifendi Muhammad Ikbal Nurul Aulia Rahmi Putri, Riri Rahmadani Quratul Akyuni Rahmawati Darussyamsu Ramadhan Sumarmin Ramadhan Sumarmin Ramadhan Sumarmin Rani Dwi Suci Hd Putri Relsas Yogica, Relsas Reny Novita Sari Rika Andima S. Syamsurizal Selaras, Ganda Hijrah Titis Wulandari Tri Putri Wahyuni Velina Salsabil windi maualana putri Yerimadesi Yerimadesi Yolanda Ruhul Azomi Yul Ifda Tanjung Yulia Ratna Sari Zuhratul Mardhiyah Amir