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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Jurnal Agroekoteknologi Buletin Kebun Raya BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Kelapa Sawit Journal of Tropical Crop Science JURNAL BUDIDAYA PERTANIAN Berkala Penelitian Agronomi Buletin Palma Indonesian Journal of Applied Research (IJAR) International Journal of Agriculture System
Diny Dinarti
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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Validasi Metode Analisis Zeatin Menggunakan Teknik Ultra High Performance Liquid Chromatography (UHPLC) sebagai Instrumen Studi Kultur In vitro Kelapa Sawit (Elaeis guineensis Jacq.) Arfan Nazhri Simamora; Diny Dinarti; Sudarsono Sudarsono
Jurnal Penelitian Kelapa Sawit Vol 28 No 1 (2020): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (781.376 KB) | DOI: 10.22302/iopri.jur.jpks.v28i1.99

Abstract

Quantitative analysis of zeatin is needed to determine correlation between callus and embryo formation with the presence of zeatin itself on explant and interaction between zeatin content on explant and synthetic plant growth regulators (PGRs) which is added to oil palm in vitro culture media. This research was conducted to obtain method of zeatin content analysis that is easy and fast to reproduce, so it can be applied to research studies on phytohormones, especially zeatin. The analysis was carried out using ultra high performance liquid chromatography (UHPLC) with C18 column and isocratically 100% methanol mobile phase and 0.2 mL / minute flow rate with UV detector at length λ = 280 nm. Analyzed explants were taken from individuals representing La Me, Avros, PPKS 239 and Simalungun varieties. Results of system suitability test indicated that the method fulfilled its requirements to be used for analysis with relative standard deviation (RSD) value was ≤ 2. Method validation gaveresults of calibration curve with the coefficient of determination r2 = 0,9996 and precision of instrument and method obtained relative standard deviation by 0,56 and 1,03, respectively. LoD and LoQ values ​​obtained were 2,457 ppm and 7,446 ppm respectively.
DNA Fingerprinting Analysis of Oil Palm In Vitro Calli Using Simple Sequence Repeats (SSR) Markers Arfan Nazhri Simamora; Diny Dinarti; Sudarsono Sudarsono; Sri Wening
JURNAL BUDIDAYA PERTANIAN Vol 18 No 1 (2022): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2022.18.1.81

Abstract

Propagating elite oil palm (Elaeis guineensis Jacq.) planting material through in vitro culture techniques requires more time and advanced technique. Early detection of culture stability would facilitate the process of culture selection and maintenance. This research aimed to analyze the DNA fingerprinting of explants and their calli. Calli consisted of embryogenic and non-embryonic calli, which had been subcultured three times. DNA of explants and calli isolated with DNeasy® Plant mini kit (Qiagen) and Genomic DNA Mini Kit (Plant) (Geneaid). DNA was amplified by SSR-PCR using 16 SSR markers, and can be bulked into two groups to save analysis cost.The result showed that 16 markers produced electropherograms that were identical between the explant and calli. Relatedness coefficient indicated that both compared explant and calli were genetically identical (r = 1). The markers used were quite informative with an average PIC number = 0.48 and can be used for DNA fingerprinting analysis of oil palm in vitro culture.
Meristem Culture Method for Virus Elimination from Shallot Bulb Prabawati Hyunita Putri; Diny Dinarti; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.185-189

Abstract

Meristem Culture Method for Virus Elimination from Shallot Bulb Shallot farmers in Indonesia have commonly grown bulbs from the previous planting season as seed sources for the next season. This may cause the accumulation of viruses in bulbs which in turn lowering plant productivity. A research was conducted to optimize the meristem culture method combined with thermotherapy (30 ºC) to produce virus-free shallot bulbs. Shallot bulbs of cv. Bima Curut that had been confirmed infected by Potyvirus and Carlavirus were used as explant sources. Bulb sterilization was carried out using bactericides, fungicides, and sodium hypochlorite. Meristem shoots of 0.6 to 0.7 mm in size were isolated from bulbs and plant successively in the shoot, root, and bulb induction medium to form mini bulbs. The mini bulbs were then planted in the screen house for 2 to 2.5 months until micro bulbs were formed. Virus detection by reverse transcription-polymerase chain reaction method confirmed that plantlets at the multiplication stage and plants in the screen house were free of Potyvirus and Carlavirus infestations. This study showed that the meristem culture method combined with thermotherapy is a potential approach for producing virus-free shallot bulbs.
Identifikasi Karakter Agronomi dan Fisiologi Daun Beberapa Genotipe Mutan Ubi Kayu (Manihot esculenta Crantz) Wening Kusuma Wardani; Nurul Khumaida; Diny Dinarti
Buletin Agrohorti Vol. 11 No. 1 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i1.46576

Abstract

Indonesia telah menjadikan daun ubi kayu sebagai salah satu sayur pilihan dalam konsumsi sehari-hari. Hal ini menjadikan peningkatan kualitas dan kuantitas daun ubi kayu perlu dilakukan. Departemen Agronomi dan Hortikultura, Fakultas Pertanian IPB telah memiliki beberapa genotype ubi kayu mutan stabil hasil iradiasi sinar gamma berpotensi sebagai varietas baru ubi kayu penghasil daun untuk sayuran. Tujuan percobaan adalah untuk mempelajari karakter agronomi dan fisiologi daun beberapa genotipe ubi kayu serta mempelajari korelasi antara tingkat hijau daun dengan hasil klorofil meter SPAD (Soil Plant Analysis Development). Penelitian menggunakan rancangan deskriptif dimana tidak ada rancangan lingkungan dan rancangan percobaan. Penilitian dilakukan dengan mengamati tinggi tanaman, lebar tanaman, indeks luad daun (ILD) daun ke-5, jumlah cabang dan tingkat hijau daun pada 35 genotipe yang berasal dari 6 tetua di lahan koleksi genotipe mutan ubi kayu. Hasil pengamatan kemudian diolah dengan menggunakan scatter plot untuk mengelompokkan genotipe unggul. Genotipe yang dinilai unggul adalah G2D12223 (tanaman asal Ratim), G3D2413 (tanaman asal UJ-5), G21533 dan G31515D (tanaman asal Gajah). Keempat genotipe kemudian diuji kandungan klorofil untuk melihat pengaruh genotipe pada klorofil dan korelasi antara tingkat hijau daun pengukuran lapangan dengan kandungan klorofil hasil uji laboratorium. Hasil uji anova dan uji lanjut menunjukkan bahwa genotipe memberikan pengaruh pada tingkat hijau daun, klorofil b, rasio klorofil a:b dan karoten. Genotipe yang dinilai unggul berdasarkan hasil tersebut adalah G31515D untuk semua karakter kecuali rasio klorofil a:b. Genotipe yang unggul dalam karakter rasio klorofil a:b adalah G21533. Hasil uji korelasi menunjukkan tingkat hijau daun berkorelasi nyata dengan total klorofil, klorofil a, klorofil b, rasio klorofil a:b dan karoten, sedangkan antosianin tidak berkorelasi dengan semua karakter uji lainnya. Kata kunci: cassava, dikotil, genotipe, karoten, klorofil
Manajemen Panen dan Pasca Panen Edible Flower di Cidadap, Kota Bandung Muhammad Reza Zakie; Diny Dinarti; Agus Purwito
Buletin Agrohorti Vol. 11 No. 1 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i1.46577

Abstract

Kegiatan penelitian telah dilaksanakan di Kecamatan Cidadap, Kota Bandung dari 27 Januari hingga 25 Mei 2019. Tujuan dari pelaksanaan penelitian ini adalah mengetahui permasalahan panen dan pasca panen edible flower di perusahaan dan dapat menemukan cara penanganan pasca panen yang baik. Edible flower memerlukan aspek pasca panen yang tepat agar tetap terjaga kesegaran dan penampilan bunga hingga sampai di tangan konsumen. Aspek teknis yang dilakukan adalah pemasangan instalasi NFT, pembuatan media tanam, persemaian, penanaman, pemeliharaan, panen, dan pasca panen. Aspek yang dipelajari bagian pengemasan akhir edible flower. Pengujian daya simpan pada tiga jenis edible flower Pansy (Viola x wittrockiana), Viola (Viola tricolor), dan Dianthus (Dianthus caryophillus) pada suhu 10 ℃ dan 20 ℃. Ketiga jenis bunga ini dapat mempertahankan kualitas hingga tujuh hari di penyimpanan suhu 10 ℃ hari dibanding dengan suhu 20 ℃ yang hanya bertahan hingga hari ketiga setelah panen. Kata kunci : hidroponik NFT, kualitas edible flowers, penyimpanan
Pengaruh Giberelin (GA3) terhadap Pertumbuhan dan Komponen Hasil Bawang Merah (Allium cepa var. aggregatum) Varietas Bima Brebes Fahmi Muhammad Cokrosudibyo; Diny Dinarti; Syarifah Iis Aisyah
Buletin Agrohorti Vol. 11 No. 2 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i2.46936

Abstract

Bawang merah (Allium cepa var. aggregatum) banyak dibudidayakan petani di Indonesia karena memiliki pemanfaatan yang cukup meluas. Penggunaan ZPT merupakan faktor pendukung yang memberikan kontribusi dalam peningkatan produktivitas bawang merah. Salah satu ZPT tersebut ialah giberelin atau GA3. Penelitian ini bertujuan untuk mengetahui pengaruh GA3 pada bawang merah varietas Bima Brebes, dan mendapatkan dosis optimum sehingga diharapkan dapat meningkatkan produktivitas. Penelitian ini dilaksanakan pada bulan Maret sampai Mei 2020 di Kabupaten Brebes. Penelitian dilakukan dengan cara foliar spray GA3 dengan 0–2,5 dosis dalam volume semprot 400 L air pada tanaman bawang merah varietas Bima Brebes. Rancangan yang digunakan adalah Rancangan Kelompok Lengkap Teracak faktor tunggal 4 ulangan, terdiri dari 6 perlakuan dosis masing-masing yakni 0; 0,5; 1,0; 1,5; 2,0; dan 2,5 dosis GA3. Hasil penelitian menunjukkan bahwa 0,5–2,0 dosis GA3 secara nyata berhasil meningkatkan tinggi tanaman, bobot 10 umbi, bobot kering per tanaman dan bobot per petak yang memengaruhi dugaan hasil per hektar. Adapun dosis optimum dari hasil analisis regresi menunjukkan pada parameter tinggi tanaman 4–6 MST berturut- turut yakni 1,48; 1,65 dan 1,88 dosis GA3, sedangkan untuk bobot 10 umbi yakni 1,54 dosis GA3. Kata kunci: bawang merah, bibit umbi, Bima Brebes, foliar spray, GA3
Performance and Relationship of Various Local Garlic Genotypes and Putative Mutants Resulting from MV4 Irradiation Using Morphological Marker Yusup Bahrul Ulum; Diny Dinarti; Sudarsono
Indonesian Journal of Applied Research (IJAR) Vol. 4 No. 2 (2023): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v4i2.357

Abstract

Garlic (Allium sativum L.) is used as a medicinal plant or seasoning, but its national production can only meet people's needs by 6.4%, and 95% is imported from China. The development rate of new garlic varieties is relatively slow because garlic is generally propagated vegetatively through cloves; therefore, its diversity has become relatively narrow. Character and diversity can be improved by inducing mutations using gamma-ray irradiation. Experiments on garlic subjected to gamma-ray irradiation have shown physiological changes in the putative 3rd (MV3) mutant offspring. The experiment's 4th mutant progeny (MV4) was re-planted and compared with other local genotypes. The aim was to perform clustering based on the yielding characteristics and the intensity of the anthocyanin content. The experiment used a single-plant design with 21 experimental units, namely 16 MV4 genotypes and 5 control genotypes. The analysis of variance showed that the MV4 Lumbu Kuning 1 Gy and Ciwidey 2 Gy genotypes had better tuber weight and diameter than the other genotypes, including the control genotypes. Cluster analysis clustered garlic genotypes into five groups at a cophenetic distance 0.4. Principal component analysis (PCA) also divided the garlic genotypes into four quadrants. Genotypes in quadrant 2 had anthocyanin content intensities superior to those of other genotypes.
Pengelolaan Pemupukan di Perkebunan Pisang Cavendish Klon DM2 Lampung Timur, Lampung Syaiful Anwar; Diny Dinarti; Agus Purwito
Buletin Agrohorti Vol. 11 No. 3 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i3.48440

Abstract

Pisang merupakan buah tropis yang sangat diminati oleh masyarakat Indonesia, sehingga dibutuhkan peningkatan produksi buah pisang yang tinggi dengan kualitas yang baik melalui manajemen pemupukan. Kegiatan penelitian bertujuan mempelajari pengelolaan pemupukan pisang Cavendish klon DM2 di Lampung Timur. Penelitian dilaksanakan pada Januari hingga Mei 2019. Pengamatan pengelolaan pemupukan meliputi konsep 5T (tepat jenis, tepat dosis, tepat waktu, tepat cara, dan tepat tempat) dan evaluasi produksi. Analisis data hasil penelitian menggunakan Uji t-student. Hasil pengamatan di areal dibandingkan dengan standar dari Departemen Research and Development. Pemupukan di kebun telah memenuhi kriteria tepat kerja. Ketepatan realisasi dosis pupuk Urea, Kiesrite, dan ZnSO4 telah tepat dosis, sedangkan pupuk KCl dan Petro cas kurang tepat dosis. Realisasi waktu pemupukan belum memenuhi standar kebun. Ketepatan cara pemupukan Urea, KCl, dan Petro cas telah memenuhi kriteria, namun Kiestrite dan ZnSO4 belum memenuhi standar kebun. Semua jenis pupuk belum tepat tempat saat aplikasi kecuali pupuk ZnSO4. Umur buah rata-rata yang dipanen pada sampel blok pengamatan belum maksimal dengan lebar buah yang masih di bawah standar kebun. Jumlah sisir dan hasil bobot tandan juga masih lebih rendah dibandingkan standar kebun. Kata kunci: pisang, pupuk, produksi, tandan
Pertumbuhan Tunas Temu Putih secara In Vitro pada Beberapa Komposisi Zat Pengatur Tumbuh Amalia Nazhira; Diny Dinarti; Sudarsono
Buletin Agrohorti Vol. 12 No. 2 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i2.56863

Abstract

Rimpang jahe putih memiliki masa dormansi selama 2-3 bulan. Penelitian ini bertujuan untuk mengetahui komposisi zat pengatur tumbuh (ZPT) yang sesuai untuk perbanyakan tunas jahe putih secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan 3 AGH pada bulan Maret hingga Agustus 2022. Penelitian menggunakan rancangan acak lengkap pola faktorial (RAL) dengan 5 taraf komposisi ZPT pada media dasar MS, yaitu media MSo tanpa penambahan ZPT (kontrol); 3 ppm BAP + 0.5 ppm TDZ + 0.1 ppm NAA; 9 ppm BAP; 1.5 ppm TDZ; 3 ppm Kinetin + 0.5 ppm TDZ + 0.1 ppm NAA. Setiap perlakuan diulang sebanyak 5 kali, setiap unit percobaan terdiri dari 2 botol dan setiap botol ditanam satu eksplan. Penambahan 3 ppm BAP + 0.5 ppm TDZ + 0.1 ppm NAA dan 3 ppm Kinetin + 0.5 ppm TDZ + 0.1 ppm NAA memberikan hasil multiplikasi tunas yang paling tinggi, yaitu 6,8 tunas. Penambahan ZPT pada media tidak berpengaruh nyata terhadap pemanjangan tunas, pertambahan jumlah daun, dan pembentukan akar. Perlakuan dengan penambahan 3 ppm BAP + 0.5 ppm TDZ + 0.1 ppm NAA; 9 ppm BAP; dan 3 ppm Kinetin + 0.5 ppm TDZ + 0.1 ppm NAA menghasilkan plantlet yang menunjukkan kelangsungan hidup 100% pada masa aklimatisasi dua minggu. Kata kunci: auksin, multiplikasi, planlet, sitokinin, Zingiberaceae
Increasing Ploidy Level of Garlic (Allium sativum L.) “Tawangmangu Baru” In-Vitro Using Colchicine Molla Gebreyohannes Hailu; Ni Made Armini Wiendi; Diny Dinarti
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.122-136

Abstract

“Tawangmangu Baru” garlic variety is known to have low productivity. The variety is still highly demanded due to its strong flavour and aroma; however, its production has not yet been able to fulfill the local needs of Central Java due to the small size and limited production area. This study aimed to determine the effect of concentration and time duration of colchicine treatment towards increasing the ploidy level of “Tawangmangu Baru” garlic variety for genetic variability. The experimental design used in this study was a complete randomized design with two factorials and 12 combinations. The first factor was concentration of colchicine, i.e. 0.00, 0.02, 0.04, 0.06, 0.08 and 0.10%, and the second factor was the immersion time, i.e. 24 and 48 hours. The result indicated that, 4.72% callus induction was obtained in BDS + 0.4 mg.L-1 2,4-D + 2.0 mg.L-1 kinetin; and 4.0% callus proliferation were obtained in both BDS + 1.5 mg.L-1 2,4-D + 1.0 mg.L-1 kinetin and MS +1.5 mg.L-1 2,4-D and 1.0 mg.L-1 kinetin. The untreated plantlets showed higher mortality rate compared to the explants with 48 hours colchicine treatment. Higher number of shoots were recorded in 0.1% colchicine at 48 hours and lower shoots in 24 hours, whereas 0.1% colchicine at 24 and 48 hours showed the highest ploidy level of total nuclear DNA analyzed by flow cytometry. The genetic diversity of the “Tawangmangu Baru” garlic was successfully enhanced by colchicine and immersion treatment. Mutant lines with tetraploid and mixoploid plants were obtained. The putative lines obtained at 0.1% colchicine treatment were subcultured to produce new mutants before testing the phenotype. The application of colchicine at 24 and 48 hours treatment improved the genetic potential of “Tawangmangu Baru” garlic variety in vitro. The application of colchicine increased the ploidy level and an increase in ploidy is expected to make the bulb size larger. Larger tuber size will increase the tuber weight, and also the overall garlic productivity and production in the future.
Co-Authors , Krisantini Agus Purwito Ali Husni Ali Husni Amalia Nazhira Anas Dinurrohman Susila Aqlima , Aqlima, nFN Ardianto Mufa'adi Arfan Nazhri Simamora Arfan Nazhri Simamora ARSYAD, MIRZA ARSIATY ASMONO, DWI Atika Fathur Rahmi Awang Maharijaya Azzahra, Elmi Irmayanti Bambang S. Purwoko Cartealy, Imam Civi Cartealy, Imam Civi Chitra Priatna Cucun Yuliana dan Joko Ridho Witono Debi Rani Mutiara Dendih Sukmadijaya Devit Purwoko Dewi Citra Sari Dodo R. Sastra Donny Hartanto Dwi Murti Puspitaningtyas, Dwi Murti Efendi, Darda Efi Toding Tondok Erviana Eka Pratiwi Fahmi Muhammad Cokrosudibyo Fatsan, Ahmad Fitri Fatma Wardani Fitri Fatma Wardani Fitri Rachmawati Fitri Yulianti Fitro Adi Cahyo Halimah Widyaningrum Imam Civi Cartealy Ismail Maskromo Isna Tustiyani Joko Ridho Witono Khairiyah, Hayat Kusumawardani, Annisa Try Marai Rahmawati Maria Utami Dewi Wardhani, Maria Utami Dewi Megayani Sri Rahayu Meliyana Molla Gebreyohannes Hailu Muhamad Syukur Muhammad Alif Baharudin Muhammad Reza Zakie Ni Made Armini Wiendi Nurul Khumaida Nurul Khumaida Prabawati Hyunita Putri Purwoko, Bambang Sapto Purwoko, Devit purwoko, devit Rachmani, Inda Hidayati Rahmadara, Gemilang Rahmi Fajri Refa Yulianingsih Rusmiyati, Henny Ryan Budi Setiawan Sandra A. Aziz Sandra Arifin Aziz Sandra Arifin Aziz Sari Nurulita Sari, Rahmah Dian Shandra Amarillis Siti Shofiya Nasution Sobir Sobir SRI HENDRASTUTI HIDAYAT Sri Wening Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO, nFn Sugiyanta Suparjo Syaiful Anwar Syarifah Iis Aisyah Teuku Tajuddin Teuku Tajuddin TINCHE, nFn Ulil Azmi Nurlaili Afifah Urip Sayekti Utin Winarni Wening Kusuma Wardani Widaningsih, Ida Wika Anrya Darma, Wika Anrya Willy Bayuardi Suwarno Winarso D. Widodo Yayu Alitalia Yuliana, Cucun Yupi Isnaini Yusnita Sari Yusup Bahrul Ulum