Diny Dinarti
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia

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Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus Siti Shofiya Nasution; Diny Dinarti; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol. 13 No. 6 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.004 KB) | DOI: 10.14692/jfi.13.6.199

Abstract

Infection of Onion yellow dwarf virus (OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using combination of electrotherapy (0, 5, 10, 15, and 20 mA each for 10 minutes) and thermotherapy (23, 28, 33, 38°C each for 4 weeks). Two garlic cultivars, i.e. Sangga Sembalun and Lumbu Hijau were used as seed bulbs for OYDV elimination tests. Virus infection was confirmed using transcription-polymerase chain reaction (RT-PCR).  The result showed that thermotherapy at 33 °Cwas the best method to eliminate OYDV in garlic although the efficiency was not the same for all cultivars. The efficiency reached up to 60% for cv. Lumbu Hijau, whereas for cv. Sangga Sembalun only reached up to 40%. Electrotherapy alone or in combination with thermotherapy were not able to produce OYDV-free plantlets.
Pertumbuhan Planlet Kantong Semar (Nepenthes rafflesiana Jack.) pada Beberapa Media Tanam Selama Tahap Aklimatisasi Dendih Sukmadijaya; Diny Dinarti; Yupi Isnaini
Jurnal Hortikultura Indonesia Vol. 4 No. 3 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.86 KB) | DOI: 10.29244/jhi.4.3.124-130

Abstract

ABSTRACTThe purpose of this  research  was to determine  the effect of acclimatization media  on  N.  rafflesiana Jack. growth and to obtain the best acclimatization media. This research was conducted at Bogor Botanical Garden from June until September 2009. The research was done in  completely randomized design with threereplications. Five  kinds  media were used  as treatment,  i.e. rice   husk charcoal, cocopeat, sphagnum moss, bamboo leaf compost and combination media rice (rice husk charcoal : cocopeat = 1:1). The r esult showed that   all of the  media can be used  to  acclimate N.  rafflesiana  Jack. planlet.Key words : acclimatization, in vitro,  media combination,  Nepenthes rafflesiana ABSTRAKPenelitian  ini  bertujuan  untuk  mempelajari  pengaruh  macam  media aklimatisasi  terhadap pertumbuhan  N.  rafflesiana  Jack.  dan  untuk mendapatkan  media  yang  paling  sesuai  untuk  aklimatisasi. Penelitian dilakukan di Kebun Raya  Bogor dari bulan Juni sampai dengan September 2009. Rancangan yang digunakan dalam percobaan adalah Rancangan Acak Lengkap (RAL) dengan tiga ulangan. Lima jenis media  yang  dipergunakan sebagai  media  aklimatisasi  adalah  arang  sekam,  cocopeat,  sphagnum moss,kompos  daun  bambu dan kombinasi  media  campuran  (arang sekam  : cocopeat  = 1:1).  Hasil  percobaan menunjukkan bahwa semua media dapat digunakan untuk aklimatisasi planlet N. rafflesiana Jack.Kata  kunci: aklimatisasi, in vitro,  kombinasi media,  Nepenthes rafflesiana
Kultur Jaringan Kantong Semar (Nepenthes mirabilis) Diny Dinarti; Urip Sayekti; Yayu Alitalia
Jurnal Hortikultura Indonesia Vol. 1 No. 2 (2010): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1296.68 KB) | DOI: 10.29244/jhi.1.2.59-65

Abstract

ABSTRACTThis research was about in vitro propagation of Pitcher Plant Nepenthes mirabilis. The aims of this research were to determine: 1) the influence of kind and concentration of in vitro medium and also plant growth regulator on germination of Nepenthes mirabilis, 2)the effect of BAP and NAA on shoot multiplication of N. mirabilis. This research consisted of three experiments and all experiments used Completely Randomized Design. On the first experiment MS (Murashige and Skoog) and KC (Knudson C) were used, i.e. full (1), three-fourth (3/4), one-half (1/2) and one-fourth (1/4) Salt Concentration. On the second experiment 10 composition media were used, i.e. ½ MS, ½ MS+TDZ 0.01 ppm, ½ MS+IAA 0.1 ppm, ½ MS+GA3 10 ppm, ½ MS+150 ml coconut water, 1/4KC, 1/4 KC+TDZ 0.01 ppm, ¼KC+IAA 0.1 ppm, 1/4KC+GA3 10 ppm and 1/4KC+ 150 ml coconut water. On the third experiment BAP with 0, 0.5, 1, 2 ppm and NAA 0, 0.1, 0.2, 0.5 ppm were used as factor for shoot multiplication.The result showed that KC medium was the best medium for germination percentage of N. mirabilis (64%). MS or KC medium with ½ or ¼ salt concentration was the suitable for germinating time of N. mirabilis (average 39 days after showing). Adding of TDZ, IAA, and GA3 significantly increased germination percentage (70 – 90%) and decreased germinating time (27 – 38 days after showing). Culture growth on medium with BAP 0 - 1 ppm was the best medium for shoot and leave induction. The best medium for root growth of N. mirabilis was MS or KC medium without NAA.Key words: Nepenthes mirabilis, MS, KC, TDZ, IAA, GA3, BAP, NAA, in vitro propagation, medium
Pengaruh Iradiasi Sinar Gamma terhadap Pertumbuhan Protocorm Like Bodies Anggrek Dendrobium lasianthera (JJ. Smith) secara In Vitro Fitro Adi Cahyo; Diny Dinarti
Jurnal Hortikultura Indonesia Vol. 6 No. 3 (2015): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.083 KB) | DOI: 10.29244/jhi.6.3.177-186

Abstract

ABSTRACTThe objective of this research was to determine the effects of gamma irradiation on protocorm like bodies (PLBs) Dendrobium lasianthera and Lethal dose (LD) 30 and 50 of gamma irradiation. The irradiation was conducted at the Center of Technology Application of Isotops and Radiation, Nuclear Energy Agency (PATIR-BATAN) and culture at Tissue Culture Laboratory of IPB from February 2014 to July 2014. The treatments were arranged in a completely randomized design (CRD) with a single factor of gamma irradiation doses, include i,g. 0 Gy, 20 Gy, 40 Gy, 60 Gy, 80 Gy, and 100 Gy. Each dose of gamma irradiation treatment was repeated 5 times. There were 30 experimental units. Each experimental unit consisted of five culture bottles containing 4 individually planted PLBs Dendrobium lasianthera. The results of this research showed that the increasing doses of gamma irradiation significantly decreased the percentage of alive PLBs, PLBs germination percentage, number of leaves, number of roots, the percentage of rooted PLBs. Morphological changes among other wider and spiral leaves were observed in the treated plantlets. Lethal dose 30% (LD30) was at 19.7697 Gy and LD50 was at 67.3504 Gy.Keywords: Dendrobium lasianthera, gamma irradiation, in vitro, Lethal dose (LD), mutation ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh iradiasi gamma terhadap pertumbuhan protocorm like bodies (PLBs) anggrek Dendrobium lasianthera serta menentukan Lethal dose (LD) 30 dan 50 dari iradiasi sinar gamma. Proses iradiasi dilakukan di Pusat Aplikasi Teknologi Isotop dan Radiasi, Badan Tenaga Nuklir Nasional (PATIR-BATAN) Proses kultur dilakukan di Laboratorium Kultur Jaringan IPB. Penelitian dilakukan dari bulan Februari 2014 hingga Juli 2014. Penelitian disusun menggunakan rancangan acak lengkap (RAL) dengan faktor tunggal yaitu dosis iradiasi gamma meliputi 0 Gy, 20 Gy, 40 Gy, 60 Gy, 80 Gy, dan 100 Gy. Setiap dosis perlakuan iradiasi gamma diulang 5 kali, seluruhnya terdapat 30 satuan percobaan. Setiap satuan percobaan terdiri atas lima botol kultur yang masing-masing ditanam 4 PLBs anggrek Dendrobium lasianthera. Hasil penelitian ini menunjukkan bahwa peningkatan dosis iradiasi gamma nyata menurunkan persentase hidup PLBs, persentase PLBs berkecambah, jumlah daun, jumlah akar, dan persentase PLBs berakar. Perubahan planlet in vitro yang teramati antara lain daun melebar dan daun spiral. LD30 berada pada 19.7697 Gy dan LD50 pada 67.3504 Gy.Kata kunci: Dendrobium lasianthera, in vitro, iradiasi gamma, Lethal dose (LD), mutasi
Eliminasi Onion yellow dwarf virus melalui Kultur Meristem Tip pada Bawang Merah (Allium ascalonicum L.) Aqlima ,; Bambang S. Purwoko; Sri Hendrastuti Hidayat; Diny Dinarti
Jurnal Hortikultura Indonesia Vol. 8 No. 1 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.098 KB) | DOI: 10.29244/jhi.8.1.22-30

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ABSTRACTMeristem tip culture is culture of isolated meristem with 1-2 leaf primordia on suitable medium. This method is generally used to obtain free virus plant. Optimation of plant growth regulators (PGRs) was done to accelerate explant growth without callus formation and to avoid somaclonal variation in meristem tip culture. The aims of this study were to achieve the best combination of PGR for meristem tip growth and to evaluate meristem tip culture potential for Onion yellow dwarf virus (OYDV) elimination in shallot. This study used combination of PGRs 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) with or without 0.1 mg L-1 IAA and medium without PGR. This research consisted of two experiments conducted separately. In experiment I, cv. Bima Brebes was used and experiment II cv. Tiron was used. Each experiment was arranged in completely randomized block design with single factor (PGR combination) that has 8 combination levels and 3 replications. The result showed that medium without PGR was the most efficient for meristem tip growth. Primary shoot was growing without callus formation. RT-PCR analysis showed that all of the tested samples were still infected by OYDV. Meristem tip culture method did not eliminate OYDV in both cultivars.Keywords: Auxin, cytokinin, GA3, OYDV, RT-PCRABSTRAKKultur meristem tip merupakan kultur meristem yang diisolasi 1-2 primordia daun dan pada media yang sesuai. Metode ini umum digunakan untuk mendapatkan tanaman bebas virus. Optimasi terhadap zat pengatur tumbuh (ZPT) dilakukan untuk mempercepat pertumbuhan eksplan tanpa disertai pembentukan kalus untuk menghindari terjadinya variasi somaklonal pada kultur meristem tip. Penelitian ini bertujuan untuk mendapatkan kombinasi ZPT terbaik bagi pertumbuhan meristem tip dan untuk mengevaluasi potensi kultur meristem tip dalam mengeliminasi virus Onion yellow dwarf virus (OYDV) pada tanaman bawang merah. Penelitian ini menggunakan 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) dengan penambahan atau tanpa 0.1 mg L-1 IAA serta media tanpa ZPT. Penelitian ini terdiri atas 2 percobaan terpisah. Percobaan 1 menggunakan cv. Bima Brebes dan Percobaan 2 menggunakan cv. Tiron. Masing-masing percobaan disusun berdasarkan rancangan kelompok lengkap teracak (RKLT) dengan 1 faktor, yaitu kombinasi ZPT yang terdiri atas 8 taraf kombinasi dan 3 ulangan. Hasil yang didapat menunjukkan bahwa media tanpa penambahan ZPTmerupakan media yang paling efisien untuk pertumbuhan tunas meristem tip. Tunas utama tumbuh tanpa disertai pembentukan kalus. Hasil analisis RT-PCR menunjukkan bahwa seluruh sampel yang dideteksi masih terinfeksi OYDV. Metode kultur meristem tip belum dapat mengeliminasi virus OYDV pada kedua kultivar bawang merah.Kata kunci: Auksin, GA3, OYDV, RT-PCR, sitokinin
Genetic Diversity of Shallot (Allium cepa var. aggregatum) Based on Morphology and Molecular Markersetik Bawang Merah (Allium cepa var. aggregatum) Berdasarkan Marka Morfologi dan Molekuler Erviana Eka Pratiwi; Awang Maharijaya; Diny Dinarti
Jurnal Hortikultura Indonesia Vol. 11 No. 1 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.1.51-60

Abstract

Bawang merah di Indonesia pada umumnya diperbanyak secara vegetatif, tetapi bawang merah yang tersebar tersebut beragam bentuknya sehingga diduga memiliki keragaman genetik yang tinggi. Penelitian ini bertujuan mengidentifikasi keragaman morfologi menggunakan 21 karakter bawang merah serta keragaman molekuler menggunakan penanda RAPD. Hasil penelitian menunjukkan bahwa karakter morfologi membagi 40 genotipe bawang merah menjadi 2 kelompok utama pada koefisien ketidakmiripan 0.59 yaitu kelompok A (38 genotipe) dan B (2 genotipe), sedangkan analisis morfologi berdasarkan 14 karakter morfologi umbi menunjukkan adanya dua kelompok utama pada koefisien ketidakmiripan 0.50 yaitu kelompok A dan B, masing-masing terdiri atas 36 dan 4 genotipe. Analisis berdasarkan penanda molekuler menunjukkan dua kelompok utama pada koefisien ketidakmiripan 0.41 yaitu kelompok A (3 genotipe) dan B (37 genotipe). Marka RAPD menghasilkan 229 pita polimorfik DNA dengan total 100% dan primer informatif adalah SBN2, OPE11 dan SBN9. Pengelompokkan dari 40 genotipe dalam penelitian ini tidak berhubungan dengan asal geografis. Genotipe BM12, BM19, BM78 dan BM01 memiliki ukuran daun yang panjang, ukuran dan diameter umbi besar dan memiliki intensitas warna dasar kulit umbi kering yang gelap. BM63 dan BM24 memiliki perilaku daun yang tegak, panjang daun sedang, ukuran dan diameter umbi sedang. Genotipe potensial ini dapat dikembangkan untuk meningkatkan varietas bawang merah di Indonesia. Kata kunci: genotipe, koefisien ketidakmiripan, RAPD, primer informatif
ANALISIS BIOINFORMATIKA BERBASIS WEB PADA SEKUEN GENOM PARSIAL SAGU (Metroxylon sagu Rottb.) Devit Purwoko; Imam Civi Cartealy; Teuku Tajuddin; Diny Dinarti; Sudarsono Sudarsono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1161.678 KB) | DOI: 10.29122/jbbi.v5i1.2878

Abstract

WEB-based bioinformatic analysis on partial genome sequence of Sago (Metroxylon sagu Rottb.)ABSTRACTSago genome sequencing analysis is still very limited. This study is a preliminary study of sago sequence analysis obtained from NGS technology to understand and identify new genetic sequences that have homology to genes in the NCBI database. Sequences were analyzed using Blast2Go to determine the genetic function annotation, putative gene identification was performed on the Arabidopsis database using the BLASTx program with a 10-3 e-value limit on The Arabidopsis Information Resource (TAIR) (http://www.arabidopsis.org/index.jsp). Gene interactions were analyzed using DAVID and GeneMania programs. Based on sequence analysis with Blast2Go, 33 sequences with Blastx hit consisting of: 29 sequences had a high homology. The sago sequences with a similarity of ≥ 90% are glutamate decarboxylase and HT1-like serine threonine kinase with hit number 10. The distribution of interactions between genes from GeneMania analysis is known to be mostly interconnected in the 65.13% protein domain, predicted 19.83%, genes with 14.47% shared expression and the remaining 0.57% had localization together.Keywords: bioinformatics, gene annotation, gene ontology, genome sequence, Metroxylon sagu ABSTRAKKajian analisis sekuen genom sagu hingga saat ini masih amat terbatas. Penelitian ini merupakan riset pendahuluan analisis sekuen sagu yang diperoleh dari teknologi NGS untuk mengetahui dan mengidentifikasi sekuen gen baru yang memiliki homologi dengan gen pada database NCBI. Sekuen dianalisis menggunakan perangkat Blast2Go untuk mengetahui anotasi fungsional gen, identifikasi gen putatif dilakukan terhadap database Arabidopsis menggunakan program BLASTx dengan batas e-value 10-3 pada The Arabidopsis Information Resource (TAIR). Interaksi gen dianalisis menggunakan program DAVID dan GeneMania. Berdasarkan analisis sekuen dengan Blast2Go, diperoleh 33 sekuen dengan Blastx hit yang terdiri atas: 29 sekuen memiliki homologi yang tinggi. Gen dengan rataan kemiripan ≥ 90% adalah glutamate decarboxylase dan serine threonine-kinase HT1-like dengan jumlah hit 10. Persebaran interaksi antar gen hasil analisis GeneMania diketahui sebagian besar saling terkait pada domain protein 65,13%, koneksi yang berhasil diprediksi 19,83%, gen dengan ekspresi bersama 14,47% dan sisanya 0,57% memiliki peranan bersama. Kata Kunci: anotasi gen, bioinformatika, Metroxylon sagu, ontologi gen, sekuen genome 
PERTUMBUHAN DAN HASIL BAWANG MERAH ASAL UMBI TSS VARIETAS TUK TUK PADA UKURAN DAN JARAK TANAM YANG BERBEDA Wika Anrya Darma; Anas Dinurrohman Susila; Diny Dinarti
Agrovigor 2015: Vol 8, No 2 (2015) SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.739 KB) | DOI: 10.21107/agrovigor.v0i0.980

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Penelitian ini dilaksanakan mulai Oktober 2014 hingga Februari 2015 di Green House Kebun Percobaan IPB Cikabayan, Dramaga, Bogor. Penelitian ini bertujuan memperoleh ukuran umbi asal TSS dan jarak tanam yang optimal dalam memproduksi umbi benih bawang merah. Rancangan penelitian menggunakan Rancangan Acak Lengkap dua faktor. Faktor pertama yaitu ukuran umbi yang terdiri dari umbi berukuran sedang (diameter= 1.5-1.8 atau berbobot 5-10 g) dan besar (diameter= 1.8 cm atau berbobot 10 g). Faktor kedua yaitu jarak tanam yang terdiri dari jarak tanam 20x20 cm dan 30x30 cm. Umbi yang digunakan dalam percobaan ini merupakan umbi hasil penanaman dari biji TSS yang telah disimpan selama dua bulan. Hasil penelitian menunjukkan bahwa penggunaan umbi berukuran sedang dan besar tidak memberikan pengaruh yang nyata terhadap produksi bawang merah. Sedangkan jarak tanam memberikan hasil yang berbeda nyata. Penggunaan umbi berukuran sedang pada jarak tanam yang lebih rapat dapat meminimalkan biaya produksi dari segi penyediaan umbi benih..Kata kunci: Bawang merah, jarak tanam, ukuran umbi, umbi benih asal TSS
Kriopreservasi Tunas in Vitro Pepaya ‘Sukma’ Dengan Perlakuan Prakultur, Loading, Dan Dehidrasi Dengan Pvs2 Dan Modifikasinya Fitri Fatma Wardani; Joko Ridho Witono; Darda Efendi; Diny Dinarti
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.3999

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Papaya has high genetic variability because it is an open-pollinated plant and has genotype and phenotypeare that are easily changed due to environment changes. Cryopreservation is a storing method of germplasm in liquid nitrogen (-196 oC) which can maintain the genotype and phenotype of germplasm. The experiment aimed to obtain the best preculture, loading, and dehydration for cryopreservation of papaya ‘Sukma’ in vitro shoots. For preculture, we planted shoots on MS media with 0.3 M and 0.4 M sucrose for 1, 2, and 3 days. In the loading treatment, we immersed shoots in loading solution (liquid MS+1.2M glycerol+0.4M sucrose) for 0, 10, 20, and 30 minutes. For dehydration, we immersed shoots in cryoprotectant (PVS2 and its modification) for 5, 10, and 15 minutes. Then, shoots were immersed in liquid nitrogen. The results showed thatshoots had the best survival rate while they had been precultured on MS medium with 0.3 M sucrose for 3 days. The best loading treatment time was 20–30 minutes. The best dehydration treatment was obtained by modification of PVS2 for 10 minutes. The shoots have not been able to recovery after cryopreservation, so it can be concluded that cryopreservation of in vitro papaya ‘Sukma’ shoots has not been successful.
PENGARUH 2iP DAN AIR KELAPA TERHADAP MULTIPLIKASI TUNAS BAWANG MERAH (Allium Ascalonicum L.) KULTIVAR SUMENEP SECARA IN VITRO Chitra Priatna; Fitri Rachmawati; Diny Dinarti
Jurnal Agroekoteknologi Vol 10, No 1 (2018)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v10i1.5461

Abstract

Generative propagation of onion sumenep cultivars had not provided optimum results. To overcome this problem vegetative propagation was used with tissue culture systems. Growth Regulator (ZPT) 2iP and Coconut Water were used as a source of cytokines to stimulate explant. This research was aimed to observe effect of 2iP and coconut water in stimulating red onion compound shoots. This experiment was carried out using a completely randomized design consisting of two factors. The first factor was the provision of 2iP with five levels of concentration of 0.0, 2.0, 4.0, 6.0, 8.0 mg/l and the second factor was the provision of coconut water with three levels of concentration of 10, 20 and 30%. The parameters observed were shoot number, leaf number, leaf length, root number and root length .The results of this research showed that the combination of 8.0 mg/l 2iP and 20% coconut water resulted in an average number of shoots and the highest number of leaves, each of which was 6.5 shoots and 6.3 leaves at 8 MSP. The combination of 2iP treatment and coconut water gave more significant effect than that of the single treatment.
Co-Authors , Krisantini Agus Purwito Ali Husni Ali Husni Amalia Nazhira Anas Dinurrohman Susila Aqlima , Aqlima, nFN Ardianto Mufa'adi Arfan Nazhri Simamora Arfan Nazhri Simamora ARSYAD, MIRZA ARSIATY ASMONO, DWI Atika Fathur Rahmi Awang Maharijaya Azzahra, Elmi Irmayanti Bambang S. Purwoko Cartealy, Imam Civi Cartealy, Imam Civi Chitra Priatna Cucun Yuliana dan Joko Ridho Witono Debi Rani Mutiara Dendih Sukmadijaya Devit Purwoko Dewi Citra Sari Dodo R. Sastra Donny Hartanto Dwi Murti Puspitaningtyas, Dwi Murti Efendi, Darda Efi Toding Tondok Erviana Eka Pratiwi Fahmi Muhammad Cokrosudibyo Fatsan, Ahmad Fitri Fatma Wardani Fitri Fatma Wardani Fitri Rachmawati Fitri Yulianti Fitro Adi Cahyo Halimah Widyaningrum Imam Civi Cartealy Ismail Maskromo Isna Tustiyani Joko Ridho Witono Khairiyah, Hayat Kusumawardani, Annisa Try Marai Rahmawati Maria Utami Dewi Wardhani, Maria Utami Dewi Megayani Sri Rahayu Meliyana Molla Gebreyohannes Hailu Muhamad Syukur Muhammad Alif Baharudin Muhammad Reza Zakie Ni Made Armini Wiendi Nurul Khumaida Nurul Khumaida Prabawati Hyunita Putri Purwoko, Bambang Sapto purwoko, devit Purwoko, Devit Rachmani, Inda Hidayati Rahmadara, Gemilang Rahmi Fajri Refa Yulianingsih Rusmiyati, Henny Ryan Budi Setiawan Sandra A. Aziz Sandra Arifin Aziz Sandra Arifin Aziz Sari Nurulita Sari, Rahmah Dian Shandra Amarillis Siti Shofiya Nasution Sobir Sobir SRI HENDRASTUTI HIDAYAT Sri Wening Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO, nFn Sugiyanta Suparjo Syaiful Anwar Syarifah Iis Aisyah Teuku Tajuddin Teuku Tajuddin TINCHE, nFn Ulil Azmi Nurlaili Afifah Urip Sayekti Utin Winarni Wening Kusuma Wardani Widaningsih, Ida Wika Anrya Darma, Wika Anrya Willy Bayuardi Suwarno Winarso D. Widodo Yayu Alitalia Yuliana, Cucun Yupi Isnaini Yusnita Sari Yusup Bahrul Ulum