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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Jurnal Agroekoteknologi Buletin Kebun Raya BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Kelapa Sawit Journal of Tropical Crop Science JURNAL BUDIDAYA PERTANIAN Berkala Penelitian Agronomi Buletin Palma Jurnal Sains Agro Indonesian Journal of Applied Research (IJAR) International Journal of Agriculture System
Diny Dinarti
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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Validasi Metode Analisis Zeatin Menggunakan Teknik Ultra High Performance Liquid Chromatography (UHPLC) sebagai Instrumen Studi Kultur In vitro Kelapa Sawit (Elaeis guineensis Jacq.) Arfan Nazhri Simamora; Diny Dinarti; Sudarsono Sudarsono
Jurnal Penelitian Kelapa Sawit Vol 28 No 1 (2020): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (781.376 KB) | DOI: 10.22302/iopri.jur.jpks.v28i1.99

Abstract

Quantitative analysis of zeatin is needed to determine correlation between callus and embryo formation with the presence of zeatin itself on explant and interaction between zeatin content on explant and synthetic plant growth regulators (PGRs) which is added to oil palm in vitro culture media. This research was conducted to obtain method of zeatin content analysis that is easy and fast to reproduce, so it can be applied to research studies on phytohormones, especially zeatin. The analysis was carried out using ultra high performance liquid chromatography (UHPLC) with C18 column and isocratically 100% methanol mobile phase and 0.2 mL / minute flow rate with UV detector at length λ = 280 nm. Analyzed explants were taken from individuals representing La Me, Avros, PPKS 239 and Simalungun varieties. Results of system suitability test indicated that the method fulfilled its requirements to be used for analysis with relative standard deviation (RSD) value was ≤ 2. Method validation gaveresults of calibration curve with the coefficient of determination r2 = 0,9996 and precision of instrument and method obtained relative standard deviation by 0,56 and 1,03, respectively. LoD and LoQ values ​​obtained were 2,457 ppm and 7,446 ppm respectively.
DNA Fingerprinting Analysis of Oil Palm In Vitro Calli Using Simple Sequence Repeats (SSR) Markers Arfan Nazhri Simamora; Diny Dinarti; Sudarsono Sudarsono; Sri Wening
JURNAL BUDIDAYA PERTANIAN Vol 18 No 1 (2022): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2022.18.1.81

Abstract

Propagating elite oil palm (Elaeis guineensis Jacq.) planting material through in vitro culture techniques requires more time and advanced technique. Early detection of culture stability would facilitate the process of culture selection and maintenance. This research aimed to analyze the DNA fingerprinting of explants and their calli. Calli consisted of embryogenic and non-embryonic calli, which had been subcultured three times. DNA of explants and calli isolated with DNeasy® Plant mini kit (Qiagen) and Genomic DNA Mini Kit (Plant) (Geneaid). DNA was amplified by SSR-PCR using 16 SSR markers, and can be bulked into two groups to save analysis cost.The result showed that 16 markers produced electropherograms that were identical between the explant and calli. Relatedness coefficient indicated that both compared explant and calli were genetically identical (r = 1). The markers used were quite informative with an average PIC number = 0.48 and can be used for DNA fingerprinting analysis of oil palm in vitro culture.
Meristem Culture Method for Virus Elimination from Shallot Bulb Prabawati Hyunita Putri; Diny Dinarti; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.185-189

Abstract

Meristem Culture Method for Virus Elimination from Shallot Bulb Shallot farmers in Indonesia have commonly grown bulbs from the previous planting season as seed sources for the next season. This may cause the accumulation of viruses in bulbs which in turn lowering plant productivity. A research was conducted to optimize the meristem culture method combined with thermotherapy (30 ºC) to produce virus-free shallot bulbs. Shallot bulbs of cv. Bima Curut that had been confirmed infected by Potyvirus and Carlavirus were used as explant sources. Bulb sterilization was carried out using bactericides, fungicides, and sodium hypochlorite. Meristem shoots of 0.6 to 0.7 mm in size were isolated from bulbs and plant successively in the shoot, root, and bulb induction medium to form mini bulbs. The mini bulbs were then planted in the screen house for 2 to 2.5 months until micro bulbs were formed. Virus detection by reverse transcription-polymerase chain reaction method confirmed that plantlets at the multiplication stage and plants in the screen house were free of Potyvirus and Carlavirus infestations. This study showed that the meristem culture method combined with thermotherapy is a potential approach for producing virus-free shallot bulbs.
Identifikasi Karakter Agronomi dan Fisiologi Daun Beberapa Genotipe Mutan Ubi Kayu (Manihot esculenta Crantz) Wening Kusuma Wardani; Nurul Khumaida; Diny Dinarti
Buletin Agrohorti Vol. 11 No. 1 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i1.46576

Abstract

Indonesia telah menjadikan daun ubi kayu sebagai salah satu sayur pilihan dalam konsumsi sehari-hari. Hal ini menjadikan peningkatan kualitas dan kuantitas daun ubi kayu perlu dilakukan. Departemen Agronomi dan Hortikultura, Fakultas Pertanian IPB telah memiliki beberapa genotype ubi kayu mutan stabil hasil iradiasi sinar gamma berpotensi sebagai varietas baru ubi kayu penghasil daun untuk sayuran. Tujuan percobaan adalah untuk mempelajari karakter agronomi dan fisiologi daun beberapa genotipe ubi kayu serta mempelajari korelasi antara tingkat hijau daun dengan hasil klorofil meter SPAD (Soil Plant Analysis Development). Penelitian menggunakan rancangan deskriptif dimana tidak ada rancangan lingkungan dan rancangan percobaan. Penilitian dilakukan dengan mengamati tinggi tanaman, lebar tanaman, indeks luad daun (ILD) daun ke-5, jumlah cabang dan tingkat hijau daun pada 35 genotipe yang berasal dari 6 tetua di lahan koleksi genotipe mutan ubi kayu. Hasil pengamatan kemudian diolah dengan menggunakan scatter plot untuk mengelompokkan genotipe unggul. Genotipe yang dinilai unggul adalah G2D12223 (tanaman asal Ratim), G3D2413 (tanaman asal UJ-5), G21533 dan G31515D (tanaman asal Gajah). Keempat genotipe kemudian diuji kandungan klorofil untuk melihat pengaruh genotipe pada klorofil dan korelasi antara tingkat hijau daun pengukuran lapangan dengan kandungan klorofil hasil uji laboratorium. Hasil uji anova dan uji lanjut menunjukkan bahwa genotipe memberikan pengaruh pada tingkat hijau daun, klorofil b, rasio klorofil a:b dan karoten. Genotipe yang dinilai unggul berdasarkan hasil tersebut adalah G31515D untuk semua karakter kecuali rasio klorofil a:b. Genotipe yang unggul dalam karakter rasio klorofil a:b adalah G21533. Hasil uji korelasi menunjukkan tingkat hijau daun berkorelasi nyata dengan total klorofil, klorofil a, klorofil b, rasio klorofil a:b dan karoten, sedangkan antosianin tidak berkorelasi dengan semua karakter uji lainnya. Kata kunci: cassava, dikotil, genotipe, karoten, klorofil
Manajemen Panen dan Pasca Panen Edible Flower di Cidadap, Kota Bandung Muhammad Reza Zakie; Diny Dinarti; Agus Purwito
Buletin Agrohorti Vol. 11 No. 1 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i1.46577

Abstract

Kegiatan penelitian telah dilaksanakan di Kecamatan Cidadap, Kota Bandung dari 27 Januari hingga 25 Mei 2019. Tujuan dari pelaksanaan penelitian ini adalah mengetahui permasalahan panen dan pasca panen edible flower di perusahaan dan dapat menemukan cara penanganan pasca panen yang baik. Edible flower memerlukan aspek pasca panen yang tepat agar tetap terjaga kesegaran dan penampilan bunga hingga sampai di tangan konsumen. Aspek teknis yang dilakukan adalah pemasangan instalasi NFT, pembuatan media tanam, persemaian, penanaman, pemeliharaan, panen, dan pasca panen. Aspek yang dipelajari bagian pengemasan akhir edible flower. Pengujian daya simpan pada tiga jenis edible flower Pansy (Viola x wittrockiana), Viola (Viola tricolor), dan Dianthus (Dianthus caryophillus) pada suhu 10 ℃ dan 20 ℃. Ketiga jenis bunga ini dapat mempertahankan kualitas hingga tujuh hari di penyimpanan suhu 10 ℃ hari dibanding dengan suhu 20 ℃ yang hanya bertahan hingga hari ketiga setelah panen. Kata kunci : hidroponik NFT, kualitas edible flowers, penyimpanan
Pengaruh Giberelin (GA3) terhadap Pertumbuhan dan Komponen Hasil Bawang Merah (Allium cepa var. aggregatum) Varietas Bima Brebes Fahmi Muhammad Cokrosudibyo; Diny Dinarti; Syarifah Iis Aisyah
Buletin Agrohorti Vol. 11 No. 2 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i2.46936

Abstract

Bawang merah (Allium cepa var. aggregatum) banyak dibudidayakan petani di Indonesia karena memiliki pemanfaatan yang cukup meluas. Penggunaan ZPT merupakan faktor pendukung yang memberikan kontribusi dalam peningkatan produktivitas bawang merah. Salah satu ZPT tersebut ialah giberelin atau GA3. Penelitian ini bertujuan untuk mengetahui pengaruh GA3 pada bawang merah varietas Bima Brebes, dan mendapatkan dosis optimum sehingga diharapkan dapat meningkatkan produktivitas. Penelitian ini dilaksanakan pada bulan Maret sampai Mei 2020 di Kabupaten Brebes. Penelitian dilakukan dengan cara foliar spray GA3 dengan 0–2,5 dosis dalam volume semprot 400 L air pada tanaman bawang merah varietas Bima Brebes. Rancangan yang digunakan adalah Rancangan Kelompok Lengkap Teracak faktor tunggal 4 ulangan, terdiri dari 6 perlakuan dosis masing-masing yakni 0; 0,5; 1,0; 1,5; 2,0; dan 2,5 dosis GA3. Hasil penelitian menunjukkan bahwa 0,5–2,0 dosis GA3 secara nyata berhasil meningkatkan tinggi tanaman, bobot 10 umbi, bobot kering per tanaman dan bobot per petak yang memengaruhi dugaan hasil per hektar. Adapun dosis optimum dari hasil analisis regresi menunjukkan pada parameter tinggi tanaman 4–6 MST berturut- turut yakni 1,48; 1,65 dan 1,88 dosis GA3, sedangkan untuk bobot 10 umbi yakni 1,54 dosis GA3. Kata kunci: bawang merah, bibit umbi, Bima Brebes, foliar spray, GA3
Performance and Relationship of Various Local Garlic Genotypes and Putative Mutants Resulting from MV4 Irradiation Using Morphological Marker Yusup Bahrul Ulum; Diny Dinarti; Sudarsono
Indonesian Journal of Applied Research (IJAR) Vol. 4 No. 2 (2023): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v4i2.357

Abstract

Garlic (Allium sativum L.) is used as a medicinal plant or seasoning, but its national production can only meet people's needs by 6.4%, and 95% is imported from China. The development rate of new garlic varieties is relatively slow because garlic is generally propagated vegetatively through cloves; therefore, its diversity has become relatively narrow. Character and diversity can be improved by inducing mutations using gamma-ray irradiation. Experiments on garlic subjected to gamma-ray irradiation have shown physiological changes in the putative 3rd (MV3) mutant offspring. The experiment's 4th mutant progeny (MV4) was re-planted and compared with other local genotypes. The aim was to perform clustering based on the yielding characteristics and the intensity of the anthocyanin content. The experiment used a single-plant design with 21 experimental units, namely 16 MV4 genotypes and 5 control genotypes. The analysis of variance showed that the MV4 Lumbu Kuning 1 Gy and Ciwidey 2 Gy genotypes had better tuber weight and diameter than the other genotypes, including the control genotypes. Cluster analysis clustered garlic genotypes into five groups at a cophenetic distance 0.4. Principal component analysis (PCA) also divided the garlic genotypes into four quadrants. Genotypes in quadrant 2 had anthocyanin content intensities superior to those of other genotypes.
Potensi Mikrob Endofit dalam Menekan Penyakit Busuk Umbi pada Tanaman Bawang Merah: Potential of Endophytic Microbes in Suppressing Basal Rot Disease in Shallot Plants Sari, Rahmah Dian; Tondok, Efi Toding; Dinarti, Diny; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol. 20 No. 3 (2024): Mei 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.3.115-125

Abstract

Bawang merah adalah salah satu komoditas hortikultura unggulan di Indonesia. Salah satu kendala produksi bawang merah di Indonesia adalah penyakit busuk pangkal yang disebabkan oleh Fusarium oxysporum. Beberapa mikrob endofit telah dilaporkan perannya sebagai agens hayati dan efektif menekan penyakit tanaman. Penelitian ini bertujuan mengetahui kemampuan Bacillus siamensis, Chaetomium sp., Cuvularia lunata, dan Trichoderma asperellum dalam menekan pertumbuhan F. oxysporum. Mikrob endofit merupakan koleksi Departemen Proteksi Tanaman, Institut Pertanian Bogor. Pengujian secara in vitro dilakukan dengan metode uji koloni ganda dan uji produksi senyawa organik volatil (SOV) anticendawan dengan metode tangkup. Pengujian SOV dilakukan pada medium ADK dan TSA dengan tingkat konsentrasi, yaitu 20%, 40%, 60%, 80%, dan 100%. Uji in vivo dilakukan dengan menanam umbi bawang merah setelah direndam dalam suspensi mikrob endofit, kemudian dilakukan inokulasi F. oxysporum pada 1 minggu setelah tanam. Hasil uji koloni ganda menunjukkan hambatan sebesar 51.41% (B. siamensis), 71.04% (Chaetomium sp.), 69.45% (C. lunata), dan 74.55% (T. asperellum), sedangkan uji produksi SOV menunjukkan nilai THR yaitu 34.45% (B. siamensis), 14.53% (Chaetomium sp.), 35.23% (C. lunata), dan 42.57% (T. asperellum). Penghambatan insidensi penyakit oleh mikrob endofit pada uji in vivo berkisar 60.00% sampai dengan 73.33%.
Incidence of main viruses infecting local garlic in Java, Indonesia Hidayat, Sri Hendrastuti; Meliyana; Refa Yulianingsih; Diny Dinarti; Sari Nurulita
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.2237-15

Abstract

Virus infection is one of the challenges in garlic production due to it perpetuates from one generation to the next and its infection caused huge yield reduction. There was still few information regarding virus status on Indonesian local garlic cultivars. This study was aimed to detect four major viruses infecting local garlic in Indonesia, they were members of genus Potyvirus (Onion yellow dwarf virus/OYDV, Leek yellow stripe virus/LYSV), and Carlavirus (Garlic common laten virus/ GCLV and Shallot latent virus/SLV). Garlic samples were obtained from IPB University collection and field survey in Tegal and Karanganyar (Central Java Province). Dot immuno-binding assay (DIBA) was done for initial virus indexing on non-commercial and commercial cultivars. Reverse transcription polymerase chain reaction (RT-PCR) using four specific primers was done to detect virus on commercial cultivars. DIBA from leaf samples showed that virus incidence of OYDV was relatively higher (92.3 to 100%) than GCLV and SLV (84.6 to 100%) from all tested cultivars. On average, ‘Lumbu Hijau’ has the lowest level of virus titter (severity) than other cultivars. The virus incidence of both bulbil and single clover was similar (97 – 100%) while virus titter of OYDV, GCLV, and SLV on bulbil was the lowest than other propagation materials. Detection by RT-PCR from two commercial cultivars showed that ‘Lumbu Hijau’ has less virus incidence than ‘Jawa Lama’. LYSV, OYDV, GCLV were detected on both cultivars but SLV was not found. Further virus indexing using larger number of samples and involving more virus targets needs to be done.
The Graded Concentration of Bleach Solution and Material Size Plays an Important Role in the In Vitro Sterilization of Thyphonium flagelliforme Plants Rahmadara, Gemilang; Tajuddin, Teuku; Suparjo, Suparjo; Sobir, Sobir; Dinarti, Diny; Khairiyah, Hayat
International Journal of Agriculture System VOLUME 12 ISSUE 1, JUNE 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v12i1.5070

Abstract

The sterilization is a primary step in in-vitro culture. plant breeding through in-vitro culture hinges on the successful effectiveness of the sterilization method in producing sterile plants. This research aims to optimize sterilization methods using a completely randomized factorial design. The first factor is the size of the material (small and large), and the second factor is the graded concentration of the bleach solution. The study revealed that Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants. The application of graded bleach solution concentrations at 25%, 20%, and 15% is able to reduce contamination to 0% on small size materials and 5 % on large size materials. Material sizes do not have a significant effect on contamination level, all the same, large size materials increase the survival rate of sterilized rodent tuber plants.
Co-Authors , Krisantini Agus Purwito Ali Husni Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arfan Nazhri Simamora Arfan Nazhri Simamora ARSYAD, MIRZA ARSIATY ASMONO, DWI Awang Maharijaya Aziz, Sandra Arifin Azzahra, Elmi Irmayanti Bambang S. Purwoko Cartealy, Imam Civi Cartealy, Imam Civi Chitra Priatna Cucun Yuliana Debi Rani Mutiara Dendih Sukmadijaya Devit Purwoko Dodo R. Sastra Donny Hartanto Dwi Murti Puspitaningtyas, Dwi Murti Efendi, Darda Efi Toding Tondok Erviana Eka Pratiwi Fahmi Muhammad Cokrosudibyo Fathur Rahmi, Atika Fatsan, Ahmad Fitri Fatma Wardani Fitri Fatma Wardani Fitri Rachmawati Fitri Yulianti Fitro Adi Cahyo Hailu, Molla Gebreyohannes Halimah Widyaningrum Imam Civi Cartealy Ismail Maskromo Isna Tustiyani Joko Ridho Witono Khairiyah, Hayat Kusumawardani, Annisa Try Marai Rahmawati Maria Utami Dewi Wardhani, Maria Utami Dewi Megayani Sri Rahayu Meliyana Mufa'adi, Ardianto Muhamad Syukur Muhammad Alif Baharudin Muhammad Reza Zakie Nazhira, Amalia Ni Made Armini Wiendi Nurul Khumaida Nurul Khumaida Prabawati Hyunita Putri Purwoko, Bambang Sapto Purwoko, Devit purwoko, devit Rachmani, Inda Hidayati Rahmadara, Gemilang Rahmi Fajri Refa Yulianingsih Rusmiyati, Henny Ryan Budi Setiawan Sandra A. Aziz Sandra Arifin Aziz Sari Nurulita Sari, Dewi Citra Sari, Rahmah Dian Shandra Amarillis Siti Shofiya Nasution Sobir Sobir SRI HENDRASTUTI HIDAYAT Sri Wening Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO, nFn Sugiyanta Suparjo Syaiful Anwar Syarifah Iis Aisyah Teuku Tajuddin Teuku Tajuddin TINCHE, nFn Ulil Azmi Nurlaili Afifah Urip Sayekti Wening Kusuma Wardani Widaningsih, Ida Wika Anrya Darma, Wika Anrya Willy Bayuardi Suwarno Winarni, Utin Winarso D. Widodo Witono, dan Joko Ridho Yayu Alitalia Yuliana, Cucun Yupi Isnaini Yusnita Sari, Yusnita Yusup Bahrul Ulum