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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Jurnal Agroekoteknologi Buletin Kebun Raya BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Kelapa Sawit Journal of Tropical Crop Science JURNAL BUDIDAYA PERTANIAN Berkala Penelitian Agronomi Buletin Palma Jurnal Sains Agro Indonesian Journal of Applied Research (IJAR) International Journal of Agriculture System

Diny Dinarti

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia

Author-ID : 254212

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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Pengaruh Kombinasi Zat Pengatur Tumbuh BAP dan IAA terhadap Pertumbuhan dan Perkembangan Tanaman Daun Dewa (Gynura procumbens (Back)) dalam Kultur in Vitro Mufa'adi, Ardianto; Aziz, Sandra Arifin; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 3 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Gynura procumbens (Back) has been used for traditional medical treatment in Indonesia. Micropropagation. one of propagation methods, becomes an effective method in propagating the G. p.rocumbens (Back). The in vitro research using The group Randomize Block Design was conducted to study the growth and development of G. procumbens (Back) explant by applying combinations of BAP ( 0, /,2, and 3 ppm) and IAA (0, 0.5, and / ppm) in the Murashige and Skoog (MS) medium. The results showed combination of BAP 3 ppm and IAA 0.5 ppm was the optimum combination for the shoot multiplication, which produced 85.4 shoots per bottle. BAP 3 ppm combined with / ppm of lAA resulted in 100 % callussed culture with the largest diameter. Key words: Gynura u.rocumbens (Back), BAP, IAA, in Vitro

Induksi Umbi Mikro Tanaman Daun Dewa (Gynura pseudochina (Lour.) DC) Secara In Vitro dengan Perlakuan Sukrosa dan Daminozide Hartanto, Donny; Aziz, Sandra Ariﬁn; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The effects of sucrose and daminozide on in-vitro microtuber formation were evaluated for producing microtubers to supply year round microtubers and to facilitate sterilized explants exchange regionally and internationally. Uninodal stem segment explants were cultured on agar solidiﬁ ed Murashige and Skoog medium supplemented with 3% sucrose, 0.5 mg L-16-benzylaminopurine (BAP), 0.1 mg L-1 α-naphthaleneacetic acid (NAA) for bud multiplication for 3 months. Three nodals stem segment which already formed three perfect leaves were cultured on agar solidiﬁ ed MS medium, 3% sucrose and 1 mg L-1 NAA for two weeks to induce root formation (until ± minimum 60% of the explants rooted). The next step was the induction of three nodal stem segments for microtuber formation on agar solidiﬁ ed MS medium supplemented with 5 mg L-1 BAP and two levels of sucrose, four concentrations of daminozide for 10 weeks. Sucrose at 6% resulted in the signiﬁ cantly highest number of microtuber. The daminozide 41.29 mg L-1 stimulated tuberization at base of the stems and reduce number of microtuber formation in stolon. Keywords: daminozide, Gynura pseudochina (L.) DC, in vitro, microtuber, sucrose

Induksi Mutasi Kalus Embriogenik Gandum (Triticum aestivum L.) melalui Iradiasi Sinar Gamma untuk Toleransi Suhu Tinggi Setiawan, Ryan Budi; Khumaida, Nurul; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Mutation techniques through gamma ray irradiation is useful to support breeding programs for genetic improvement of wheat. Genetic improvement on tolerance to high temperatures is necessary for development of wheat in Indonesia. The purpose of this study was to determine the level of radiosensitivity to be used as the basis for the induction of mutations by gamma ray irradiation on embryogenic callus to obtain putative mutants with high temperature tolerance. Explants used were embryogenic callus cultured on MS medium containing 30 g L-1 sukrosa, 2 g L-1 gelrite, 2 mg L-1 2.4D and 1 mg L-1 picloram. Culture incubated for 6 weeks with temperature 20±4 oC in the room culture. Five irradiation doses (10, 20, 30, 40 and 50 Gy) were used in radiosensitivity testing. A factorial, completely randomized design was applied to the experiment. The first factor was selection temperature with three levels (25, 30 and 35 oC), and the second factor was doses of gamma ray iradiation with three levels (10, 20, and 30 Gy). The result showed that radiosensitivity levels varied among varieties, LD20: 7.79 to 18.96 Gy and LD50: 24.29-33.63 Gy. Selayar variety which has highest sensitivity value compared with Dewata and Nias. Increasing doses of iradiation and temperature decrease survival of embryogenic callus, number of embryos, and percentage of germinated plantlets. Based on in vitro selection using high temperature (25, 30, and 35 oC), the obtained 19 putative mutants were derived from embryos that appear on the surface of embryogenic callus survival after irradiation and high temperature selection.Keywords: in vitro selection, putative mutant, radiosensitivity, somatic embryo

Induksi Tetraploid Tunas Pucuk Jeruk Siam Simadu (Citrus nobilis Lour) Menggunakan Kolkisin secara In Vitro Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Seedless fruit is one of the criteria (necessary) to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is a diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines.The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0, 0.1, 0.2, and 0.3%) for 3 hours. The results showed that the high concentration of 0.3% reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine.The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplast per pair of guard cells. Compared to those of control, leaves with 0.1% colchicine had lower stomatal density, and larger stomatal size. It appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.Keywords:chloroplasts, doubling chromosomes, stomatal size, stomatal density

Ketahanan Beberapa Klon Kentang (Solanum tuberosum L.) terhadap Asam Fusarat dan Penyakit Busuk Kering Umbi Sari, Dewi Citra; Dinarti, Diny; Suwarno, Willy Bayuardi; Purwito, dan Agus
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTFusaric acid produced by Fusarium spp. played a major role in potato dry rot development. Using fusaric acid as a selection agent may be useful to identify resistant clones. The aim of this experiment was to evaluate the morphological responses of 10 potato clones (Granola, Atlantic, Cipanas, DTO 28, DTO 33, Russet Burbank, IPB 1, CIP 801040, CIP 801045, dan CIP 801050) and their resistance level to fusaric acid and Fusarium solani. The research was conducted in Plant Breeding Laboratory and Tissue Culture Laboratory 3, Departement of Agronomy and Horticulture from April 2014-February 2015. The in vitro experiment was arranged in a randomized block design with 4 fusaric acid concentrations and 4 replications, while the F. solani infection experiment was arranged in a completely randomized design with 5 replications. The result showed that fusaric acid inhibits growth, reduce microtubers production, and caused planlets death. Correlation analysis between in vitro resistance to fusaric acid and F. solani infection on tuber showed positive and notable result. Accordingly, fusaric acid can be used to identify any clones resistant to F. solani.Keywords: Fusarium solani, phytotoxin, selection

Perbanyakan Tunas Mikro pada Beberapa Umur Simpan Umbi dan Pembentukan Umbi Mikro Bawang Merah pada Dua Suhu Ruang Kultur Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 2 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Shallot bulb generally stored for several month before planted in the fi eld. Since explant age is one of important factors in tissue culture development, storage period of shallot bulb might alter the explant growth in vitro. Shoots of shallot formed in the in vitro culture should form bulbs before can be use as seedling, and temperature may affect micro bulb induction. Two experiments had been conducted to evaluate 1) the effect of storage period in the fi eld on the growth of shallot explant in vitro and 2) the effect of culture room temperature in microbulbs induction of shallot. In the fi rst experiment, shallot bulb had been stored for 1, 2, 3 and 4 months before used as explants. Storage period signifi cantly infl uenced the explant growth in vitro. Bulb with 2 months storage gave the best performance on number of micro shoot, number of leaves and roots, and less of vitrifi cation. Micro shoots on three weeks after planting (WAP) was feasible to use as propagule for shallot micro bulb induction. In the second experiment, shoots from propagation medium was transplanted to bulb induction medium and grown in growth chamber with different temperatures (day/night) 20/17 oC and 30/27 oC, respectively. Micro bulb induction was infl uenced by temperature. Lower temperature showed good results for number of leaves, length of leaves, number of roots, and length of roots. However, temperature of 30/27 oC gave the best result on number of micro bulb, diameter of bulb and bulb width:bulb disk diameter ratio. Keywords: Allium, explants age, micro bulb, temperature, 2ip

Perbanyakan Pepaya (Carica papaya L.) ‘Sukma’ In Vitro dari Eksplan Tunas Pucuk sebagai Respon terhadap BA dan NAA Wardani, Fitri Fatma; Efendi, Darda; Dinarti, Diny; Witono, dan Joko Ridho
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 2 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Papaya is one of tropical fruits native to Southern Mexico and which have been cultivated in Indonesia for a longtime. Papaya is usually propagated by seeds. Therefore, the offsprings are not true-to-type. This study was conducted todevelop a protocol of in vitro propagation of papaya ‘Sukma’ from shoot tips of in vitro germinated seeds as explants. Seedswere extracted from fruit that physiologically ripe and it germinated on MS basal medium. The experiment was set up in arandomized block design with culturing day as blocks (four blocks) and various concentrations of BA (0.0, 0.5, 1.0, 1.5, and2.0 mg L-1) in MS medium enriched with NAA 0.5 mg L-1 as treatment. Shoot tips in the MS medium without plant growthregulator as control so there was six treatments and 24 observation units. Each observation units contained five culturevessels and each culture vessels contained four explants. The results showed that the highest number of shoots, number ofleaves, number of nodes, and percentage of explant forming callus were obtained by BA 1.0-1.5, 1.0-2.0, 0.5-2.0, and 1.0-1.5 mg L-1, respectively. The highest percentage of explant forming roots were obtained in medium without BA. Analysis ofregression showed that the optimum concentration to get the highest number of shoots and leaves were BA 1.31 and 1.35 mgL-1, while explants will be rooted in medium without BA. Thus, in vitro propagation for papaya ‘Sukma’ should be conductedin two steps, i.e. for shooting and rooting growth.

Sterilisasi dan Pertumbuhan In Vitro Tunas Aksilar Pepaya Kultivar Callina dan Caliso Efendi, Darda; Dinarti, Diny; Fajri, Rahmi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 1 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Pepaya tipe sedang dan kecil sudah digemari di Indonesia sejak satu dekade lalu. Sebagian besar tanaman pepaya yang diperbanyak dari biji menghasilkan profit yang rendah karena heterogenitas dan variasi genetik yang diakibatkan oleh penyerbukan silang, sehingga diperlukan kegiatan secara kultur in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan Pusat Kajian Hortikultura Tropika (PKHT) IPB, Bogor, dari November 2018 hingga Januari 2020. Eksplan merupakan tunas aksilar pepaya kultivar Callina dan Caliso umur 2 minggu. Penelitian dirancang secara faktorial dalam lingkungan Rancangan Acak Kelompok (RAK). Penelitian ini dibagi menjadi dua percobaan: a) Percobaan pertama bertujuan untuk mengetahui pengaruh metode sterilisasi dengan faktor sumber eksplan (rumah kaca dan lapangan) dan metode sterilisasi (M1, M2, M3, dan M4), dan b) Percobaan kedua bertujuan untuk optimalisasi konsentrasi BAP dan GA3 pada pertumbuhan tunas dengan konsentrasi BAP 0.0, 0.5, 1.0, 1.5, dan 2.0 mg L-1, dan konsentrasi GA3 0.0, 0.3, dan 0.4 mg L-1. Hasil penelitian menunjukkan bahwa eksplan dari rumah kaca pada sterilisasi metode M4 menghasilkan tingkat kontaminasi terendah yaitu 19.9% untuk Caliso dan 29.9% untuk Callina. Media terbaik untuk menghasilkan tinggi tunas optimum adalah BAP 0.5 mg L-1 dan GA3 0.3 mg L-1 untuk Caliso dan BAP 0.5 mg L-1 dan GA3 0.4 mg L-1 untuk Callina dengan tinggi rata-rata 1.6 dan 1.8 cm, sehingga tunas dapat dipindahkan ke tahap pengakaran. Kata kunci: Asam giberelat, Benzyl amino purine, Carica papaya, kontaminasi, sterilisasi

Evaluasi Metabolomik Mutan Putatif Bawang Putih (MV3) Hasil Iradiasi Gamma LD50 Syarifah Iis Aisyah; Winarni, Utin; Diny Dinarti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 1 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Peningkatan keragaman genetik bawang putih dapat melalui pemuliaan mutasi buatan menggunakan sinar gamma. Penelitian bertujuan untuk mempelajari pengaruh dosis iradiasi gamma pada LD50 terhadap pertumbuhan, produksi, dan kandungan senyawa metabolit generasi MV3 tiga genotipe bawang putih. Penelitian dilaksanakan pada bulan Mei sampai September 2020 di Kebun Percobaan Pasir Sarongge IPB. Percobaan terdiri atas enam populasi mutan bawang putih tanpa ulangan, yaitu Ciwidey 0 Gy , Ciwidey 6 Gy, Tawangmangu Baru 0 Gy, Tawangmangu Baru 8 Gy, Lumbu Kuning 0 Gy, Lumbu Kuning 10 Gy. Setiap populasi memiliki jumlah individu yang berbeda tergantung ketersediaan bahan tanam dari generasi sebelumnya. Total 364 tanaman yang diuji tersebut diamati pertumbuhan dan produksi tanaman. Hasil penelitian paparan dosis LD50 pada semua genotipe bawang putih menurunkan diameter umbi, jumlah siung dibandingkan kontrol. Senyawa baru yang terdeteksi pada CW 6 Gy yaitu 2-Hexanol; 2-Hexanol, 3,4-dimethyl-; Cyclopentene, 1,2,3,3,4-pentamethyl-; Trisulfide, methyl 2-propenyl. Senyawa baru yang terdeteksi pada LK 10 Gy yaitu Butyl isobutyl phthalate; 2-Pentanol, 2-methyl-; Ether, 2-chloro-1-methylethyl isopropyl; Hentriacontane; Isoamyl lactate. Senyawa baru yang terdeteksi pada TMB 8 Gy yaitu; Cyclopentene, 1,2,3,3,4-pentamethyl-; Decane; 3-Vinyl-1,2-dithiacyclohex-4-ene; Diallyl disulphide. Kata kunci: allicin, antimikroba, GC-MS, metabolomik, mutan

Pengelolaan Pemupukan di Perkebunan Pisang Cavendish Klon DM2 Lampung Timur, Lampung Anwar, Syaiful; Dinarti, Diny; Purwito, Agus
Buletin Agrohorti Vol. 11 No. 3 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i3.48440

Pisang merupakan buah tropis yang sangat diminati oleh masyarakat Indonesia, sehingga dibutuhkan peningkatan produksi buah pisang yang tinggi dengan kualitas yang baik melalui manajemen pemupukan. Kegiatan penelitian bertujuan mempelajari pengelolaan pemupukan pisang Cavendish klon DM2 di Lampung Timur. Penelitian dilaksanakan pada Januari hingga Mei 2019. Pengamatan pengelolaan pemupukan meliputi konsep 5T (tepat jenis, tepat dosis, tepat waktu, tepat cara, dan tepat tempat) dan evaluasi produksi. Analisis data hasil penelitian menggunakan Uji t-student. Hasil pengamatan di areal dibandingkan dengan standar dari Departemen Research and Development. Pemupukan di kebun telah memenuhi kriteria tepat kerja. Ketepatan realisasi dosis pupuk Urea, Kiesrite, dan ZnSO4 telah tepat dosis, sedangkan pupuk KCl dan Petro cas kurang tepat dosis. Realisasi waktu pemupukan belum memenuhi standar kebun. Ketepatan cara pemupukan Urea, KCl, dan Petro cas telah memenuhi kriteria, namun Kiestrite dan ZnSO4 belum memenuhi standar kebun. Semua jenis pupuk belum tepat tempat saat aplikasi kecuali pupuk ZnSO4. Umur buah rata-rata yang dipanen pada sampel blok pengamatan belum maksimal dengan lebar buah yang masih di bawah standar kebun. Jumlah sisir dan hasil bobot tandan juga masih lebih rendah dibandingkan standar kebun. Kata kunci: pisang, pupuk, produksi, tandan

Co-Authors , Krisantini Agus Purwito Ali Husni Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arfan Nazhri Simamora Arfan Nazhri Simamora ARSYAD, MIRZA ARSIATY ASMONO, DWI Awang Maharijaya Aziz, Sandra Ariﬁn Azzahra, Elmi Irmayanti Bambang S. Purwoko Cartealy, Imam Civi Cartealy, Imam Civi Chitra Priatna Cucun Yuliana Debi Rani Mutiara Dendih Sukmadijaya Devit Purwoko Dodo R. Sastra Donny Hartanto Dwi Murti Puspitaningtyas, Dwi Murti Efendi, Darda Efi Toding Tondok Erviana Eka Pratiwi Fahmi Muhammad Cokrosudibyo Fathur Rahmi, Atika Fatsan, Ahmad Fitri Fatma Wardani Fitri Fatma Wardani Fitri Rachmawati Fitri Yulianti Fitro Adi Cahyo Hailu, Molla Gebreyohannes Halimah Widyaningrum Imam Civi Cartealy Ismail Maskromo Isna Tustiyani Joko Ridho Witono Khairiyah, Hayat Kusumawardani, Annisa Try Marai Rahmawati Maria Utami Dewi Wardhani, Maria Utami Dewi Megayani Sri Rahayu Meliyana Mufa'adi, Ardianto Muhamad Syukur Muhammad Alif Baharudin Muhammad Reza Zakie Nazhira, Amalia Ni Made Armini Wiendi Nurul Khumaida Nurul Khumaida Prabawati Hyunita Putri Purwoko, Bambang Sapto purwoko, devit Purwoko, Devit Rachmani, Inda Hidayati Rahmadara, Gemilang Rahmi Fajri Refa Yulianingsih Rusmiyati, Henny Ryan Budi Setiawan Sandra A. Aziz Sandra Arifin Aziz Sari Nurulita Sari, Dewi Citra Sari, Rahmah Dian Shandra Amarillis Siti Shofiya Nasution Sobir Sobir SRI HENDRASTUTI HIDAYAT Sri Wening Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO, nFn Sugiyanta Suparjo Syaiful Anwar Syarifah Iis Aisyah Teuku Tajuddin Teuku Tajuddin TINCHE, nFn Ulil Azmi Nurlaili Afifah Urip Sayekti Wening Kusuma Wardani Widaningsih, Ida Wika Anrya Darma, Wika Anrya Willy Bayuardi Suwarno Winarni, Utin Winarso D. Widodo Witono, dan Joko Ridho Yayu Alitalia Yuliana, Cucun Yupi Isnaini Yusnita Sari, Yusnita Yusup Bahrul Ulum

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