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Elfi Anis Saati
Universitas Muhammadiyah Malang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Antioxidant Activity Test of Black Rice (Oryza sativa L. Indica) Extract and Total Phenolic Test of Soybean Sprout (Glycine max L.) Extract Using 70%, 90%, and 96% Ethanol Variations. Maulizah, Rizlah; Kintoko; Nina Salamah; Elfi Anis Saati
Jurnal FARMASIMED (JFM) Vol 8 No 2 (2026): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/v5bg4116

Abstract

Background: The human body requires external antioxidants to neutralize free radicals that cause cellular damage and degenerative diseases. Black rice contains higher anthocyanin levels than red rice, while soybean germination increases flavonoids, phenolic compounds, and secondary metabolites.Objective: This study aimed to determine the total phenolic content of germinated soybeans extracted using three ethanol solvent concentrations (70%, 90%, and 96%) and to valuate the antioxidant activity of soybean and black rice extracts as copigmentation agents. Methods: Soybean extracts were obtained by maceration using different ethanol concentrations, followed by analysis of total phenolic content and antioxidant activity. Black rice extract was evaluated for antioxidant activity using the DPPH method to obtain a stable antioxidant with a very strong IC₅₀ value. Results: The total phenolic content of germinated soybean extracts varied with solvent concentration: ethanol 70% yielded 2.24 mg GAE/g, ethanol 90% yielded 7.14 mg GAE/g, and ethanol 96% yielded 3.36 mg GAE/g. The total anthocyanin content of black rice extract determined by the pH differential method was 8.182 mg/100 g, with an antioxidant activity IC₅₀ of 82.24 ± 3.6 ppm, categorized as strong. The IC₅₀ value of the non-copigmented sample was 83.71 ± 1.6 ppm (moderate), while copigmentation ratios of 1:1, 1:2, and 1:3 resulted in IC₅₀ values of 70.83 ± 0.8 ppm (strong), 62.97 ± 1.2 ppm (strong), and 38.53 ± 0.3 ppm (very strong), respectively. Conclusion: Extraction of germinated soybeans using ethanol 90% significantly increased total phenolic content (p < 0.05). Copigmentation of black rice anthocyanin flavylium with germinated soybean polyphenols at a 1:3 ratio produced very strong antioxidant activity (p < 0.05), indicating its potential as a copigmentation agent in functional food products.
Co-Authors A Aulanni'am Abd. Haris Afifa Husna Agustin, Wida Ayunindya Ahmad Wahyudi Andriawan, Sony Aulanni'am, Aulanni'am Daely, Brian Fo’era-era Damat, Damat Desiana Nuriza Putri Devi Dwi Siskawardani Dewi, Abrienda Jessika Dian Fitri Argarini, Dian Fitri Dwi Budiono Fatin, Naila Salsabila Nurul Febrinasari, Salsabilla Fika Treeza Putri Fina Innama Listin Gilang Yoga Pratama Hamida, Maretha Hanif Alamudin Manshur Haprinata, Edwin Eka Heidir, Hilwa Herlambang, Alexander Husna, Afifa Iwan Nuddin Jamroji Jamroji Khusnul Khotimah Kinasih, Anggraini Kintoko Lulu Wulandini M.Sc, Desiana Nuriza Putri Maulizah, Rizlah Mika Ambarawati, Mika MISTIANAH, MISTIANAH Mochammad Wachid MP Editor, Ir. Dian Indratmi, MP Murni, Lissa Nelis Oetavi A. Nina Salamah Nisa Rahmawati Nora Idiawati Noviani, Auliarinda Nur Sriwijayanti Nurhakim Nurhakim Nurkhozin Azis Nurkhozin Azis, Nurkhozin Rahmadani, Yesika Ririn Harini Rista Anggriani Roberto Danieli Ruli Inayah Ramadhoan Safitri, Adella Audina Samsoedin, Fadhila Marwa Samudra, Norazkya Mutiara Silvia Khilmi SIMON BAMBANG WIDJANARKO Sri Wahyuni Sri Winarsih Sri Winarsih Theovilla RRD THEOVILLA RRD, THEOVILLA Tri Mahdiyahtul Faricha Trio Ageng Prayitno, Trio Ageng Vina Salviana Darvina Soedarwo Vritta Amroini Wahyudi Wachid, Moch. Wachid, Muhammad Warkoyo .