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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner YARSI Medical Journal Jurnal Kedokteran Hewan
KARTINI ERIANI
Department of Biology, Faculty of Mathematics and Natural Sciences of Syiah Kuala University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers Eriani, Kartini; Hasanah, Uswatun; Fitriana, Rizky; Sari, Widya; Yunita, Yunita; Azhar, Al
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30080

Abstract

Providing scientific evidences for the medicinal benefits and possible toxic effects of the flamboyant flowers (Delonix regia (Bojer ex Hook.) Raf.) are very important to implementing the medicinal plant in this modern era. This study aimed to investigate antidiabetic potential of methanol extract of flamboyant flowers using a completely random design. Twenty-four male rats were randomly divided into 6 treatment groups with 4 replications: healthy rats (normal control, P1), diabetic rats treated with flamboyant flower extract of 0 (negative control, P2), 100 (P3), 200 (P4), and 400 mg/kg BW (P5), and rats which were induced by 0.45 mg/kg BW of Glibenclamide (positive control, P6). Diabetic condition was achieved by a single injection of alloxan 150 mg/kg BW. Treatments were given once a day for 14 days. On day 0, 3 and 18 blood samples were withdrawn from rats’ orbital vein for glucose measurement. All rats were sacrificed for liver, gastrocnemius muscles and pancreatic tissues collection. The liver and gastrocnemius muscle were subjected for glycogen measurement whereas pancreatic tissues were processed for histological examinations. Data was analyzed by ANOVA and followed by Duncan test. The results showed that flamboyant flowers extracts significantly (p<0.05) reduced blood glucose as well as degeneration and necrosis of pancreatic β cells. Optimal dose to decrease blood glucose and pancreatic cell degeneration was 200 mg/kg BW; whereas optimum dose to decrease pancreatic cell necrosis was 400 mg/kg BW. In conclusion, flamboyant flower extract can reduce blood glucose in rats. This is the first that shows antidiabetic potential of local Flamboyant flower extracts along its toxicity effect to pancreatic tissues. These information could become a basic consideration for the use of the plant extracts as a candidate to cure patients with diabetic problems.
Effect of Sperm Extender and Dilution Ratio on The Sperm Motility, Fertility and Hatchability of Depik fish Rasbora Tawarensis (Pisces: Cyprinidae) Eggs Muchlisin, Zainal Abidin; Aldila, Dhea Febby; Sarah, Putri Inten; Eriani, Kartini; Hasri, Iwan; Fadli, Nur; Muhammadar, Abdullah Abbas; Siti-Azizah, Mohd Nor; Kocabas, Filiz Kutluyer
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 20, No 2 (2024): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.20.2.%p

Abstract

The artificial breeding method for depik fish Rasbora tawarensis was not available, and the extenders and its dilution ratio are the crucial factors in artificial breeding of fishes. Herein, we tested several common sperm extenders and its dilution ratio  in the artificial breeding for depik fish. Two  series of experiments were performed to find the best extender and its dilution ratio. In the first experiment, five types of extenders, namely: Ringer’s solution, physiological solution, coconut water, sugarcane water, and roomie water. The second experiment studied four dilution levels of sperm and extender (v/v), namely: 1: 20, 1: 30, 1: 40, and 1: 50. Every treatment was conducted in four replicates and experiments were conducted in series so that the best results in the first experiment were used in the second.  The first experiment showed that Ringer’s is the best extender for depik sperm, and the second experiment revealed that Ringer’s at dilution ratio 1: 40 gave higher sperm motility, fertilization and hatching rates. Hence,  the best formula for the dilution solution of depik sperm is Ringer’s at the dilution ratio of 1: 40, sperm: extender (v/v).
Amelioration of Seminal Plasma Testosterone Concentration in Gembrong Goats after In Vivo Administration of PGF2α Hafizuddin, Hafizuddin; Husnurrizal, Husnurrizal; Siregar, Tongku Nizwan; Eriani, Kartini; Wahyuni, Sri; Ahsan, Muhammad Maulana; Sutriana, Amalia; Anwar, Anwar; Aliza, Dwinna
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.256-261

Abstract

The semen quality of Gembrong goats is lower than other goats and may be related to the low concentration of testosterone hormone. Implementation of reproductive technology using prostaglandin F2α (PGF2α) hormone is beneficial to increase the testosterone hormone in Gembrong goats. This study aimed to determine the effect of PGF2α injection on increasing testosterone levels in Gembrong goats. Male Gembrong goats (n=4), aged 2.5-4 years with similar body condition scores (BCS=3) were used in this study. Goats were divided into two treatment groups (n=2). Goats in group 1 (G1) were injected intramuscularly with 1 ml PGF2α (75 μg), while those in group 2 (G2) were injected with 1 ml physiological NaCl. Semen collection was carried out 30 minutes after treatment using an artificial vagina. Testosterone levels were measured using the enzyme-linked immunosorbent assay (ELISA). The collected data was tabulated and analyzed descriptively. The results showed that the average testosterone concentration of G1 was higher than G2 with respective concentrations of 6.41 ± 0.70 and 2.81 ± 1.75 ng/ml. It was concluded that administration of PGF2α in vivo could increase testosterone concentration in Gembrong goats.
MOLECULAR ANALYSIS OF PBMSP-1 GENE IN ERYTHROCYTE OF MICE INFECTED WITH Plasmodium berghei Rosnizar, Rosnizar; Eriani, Kartini
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.7452

Abstract

This study aimed to analyze MSP-1 gene in mice erythrocyte infected with Plasmodium berghei using polymerase chain reaction (PCR). The result showed the existence of 462 bp DNA band which was assumed to encode MSP-1 protein with molecular weight of 19 kDa that can only be found in erythrocyte infected with Plasmodium berghei. BLASTn analysis showed that PbMSP-1 obtained in this study have 100% similar identity with mRNA of PbMSP-1 partial sequence (462 bp), 93% similarity with PbMSP-1 complete sequence (5750 bp and 5376 bp), and 87% similarity with PbMSP-1 incomplete sequence (333 bp).
PRODUKSI EMBRIO KUCING SECARA IN VITRO DARI SPERMATOZOA HASIL PRESERVASI MELALUI FERTILISASI MIKRO Eriani, Kartini; Sukra, Yuhara; Boediono, Arief; Djuwita, Ita; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.563

Abstract

Penelitian ini bertujuan mengetahui motilitas dan kemampuan memfertilisasi sperma kucing yang berasal dari epididimis yang disimpanpada suhu 4 C. Epididimis disimpan dalam media phosphate buffer saline (PBS) pada suhu 4 C selama 1, 3, dan 6 hari. Viabilitas spermatozoa diamati dengan pewarnaan hoechst-propidium iodine. Fungsi biologis spermatozoa dievaluasi melalui teknik kultur in vitro dengan fertilisasi mikro dan perkembangan embrio di dalam media kultur CR1aa. Hasil penelitian menunjukkan persentase spermatozoa hidup pada hari ke-1, 3, dan 6 penyimpanan masing-masing adalah 81,0; 71,7; dan 70,7% (duktus deferens), 84,0; 81,2; dan 63,2% (kauda epididimis), 84,0%; 75,0; dan 74,7% (korpus epididimis). Persentase pronukleus (PN) yang didapat dengan teknik intra cytoplasmic sperm injection (ICSI) menggunakan spermatozoa epididimis pada hari ke-1, 3, dan 6 hari penyimpanan masing-masing adalah 8,0; 10,0; dan 5,9%. Preservasi epididimis pada suhu 4 C dalam PBS dapat digunakan untuk fertilisasi dan menghasilkan embrio kucing secara in vitro.
Co-Authors A.A. Ketut Agung Cahyawan W Ahsan, Muhammad Maulana Al Azhar AL-AZHAR AL-AZHAR Aldila, Dhea Febby Amalia Sutriana Anwar Anwar Arief Boediono Bintoro, Yudha Dasrul Dasrul Deby Anggraini, Deby Dwinna Aliza Dwitya, Yonadiah Fitriana, Rizky Hafizuddin Hafizuddin Hendra Saputra Husnurrizal, Husnurrizal I. Djuwita Ichsan Ichsan Irma Suryani ITA DJUWITA Iwan Hasri Kocabas, Filiz Kutluyer M. Nasir Mahfudhah, Raudhah MUHAMMAD RIZAL Muhammadar, Abdullah Abbas Nur Fadli Nursanti, Risa Pusaka, Semerdanta Rosnizar, Rosnizar Sarah, Putri Inten Sari, Nisa SILMI MARIYA Siti-Azizah, Mohd Nor SONY HERU SUMARSONO Sri Wahyuni Syahril, Alfis Tongku Nizwan Siregar Uswatun Hasanah Widya Sari Yasmin, Cut Yasmin, Cut Yuhara Sukra Yunita Yunita ZAINAL ABIDIN MUCHLISIN