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Detection of Dengue Virus Serotypes Using RT-PCR from Jakarta, Indonesia Widiani, Nurhaida
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 5 No. 2 (2025): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/b7v64334

Abstract

This research was conducted to identify dengue virus serotypes circulating in Jakarta using the RT-PCR method. A total of 30 blood samples were used to study dengue virus serotypes circulating in Jakarta. The results of the study showed that all four dengue virus serotypes were found in Jakarta. Apart from that, cases of double infection with the dengue virus have also been found in the area, which can worsen the symptoms of the disease. RT-PCR has proven effective in detecting dengue virus with a high level of sensitivity and specificity. Early detection of the dengue virus is considered crucial in efforts to prevent and control this disease
Effectiveness of Jatropha multifida L. Leaf Extract Gel on Incision Wound Healing in Male Mice (Mus musculus) Rudini, Mahmud; Widiani, Nurhaida; Febriyani, Uun; Rahayu, Regita Lestari
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 5 No. 2 (2025): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/2yckgp97

Abstract

Jatropha multifida L. has been used as a traditional remedy for fresh wounds. Its bioactive compounds, including flavonoids, alkaloids, saponins, and tannins, are known for their potential to accelerate wound healing. Research on medicinal plants continues to advance in order to develop more practical pharmaceutical formulations. This study aimed to assess the effectiveness of Jatropha multifida L. leaf extract gel on incision wound healing in Mus musculus and to identify the optimal concentration. A laboratory experimental method with a Completely Randomized Design was used, consisting of five treatments: negative control (no gel), positive control (Bioplacenton), and extract gel concentrations of 15%, 30%, and 45%, each replicated three times using 15 male mice. Daily observations over 25 days included measurements of wound length, redness, scab formation and detachment, and overall wound closure. Data were analyzed using ANOVA to determine differences among treatment groups. One-way ANOVA results showed a significance value of < 0.05, indicating a meaningful effect on wound reduction. The 45% gel concentration demonstrated the highest effectiveness in promoting wound healing. This outcome is linked to the flavonoid content of Jatropha multifida, which supports vasodilation, collagen synthesis, and the strengthening of collagen fibers during the healing process.  
The Effect of Liquid Organic Fertilizer of Stale Rice and Sediment of Catfish Ponds on the Growth of Chili Plants (Capsicum annuum L.) Winandari, Ovi Prasetya; Anisya, Shinta; Marantika, Sapta; Widiani, Nurhaida; Novitasari, Aulia
JURNAL BIOLOGICA SAMUDRA Vol 7 No 2 (2025): Biologica Samudra
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jbs.v7i2.11732

Abstract

riculture in Indonesia produces various types of plants such as staple foods such as rice, corn and vegetables. Vegetables that contain a lot of nutrients include chili plants. The productivity of chili plants in Indonesia is still relatively low, one of the factors is the low soil fertility due to the use of inorganic fertilizers. Therefore, alternative organic fertilizers are made from restaurant/household waste, one of which is rice waste and sediment water from catfish ponds that can be recycled. This study aims to evaluate the impact of using liquid organic fertilizer (LOF) derived from fermented rice and catfish pond sediment on the growth of curly red chili plants (Capsicum annum L.). The research utilized a completely randomized design (CRD) with a factorial model comprising two factors. The first factor was LOF made from fermented rice (Factor M), and the second was LOF from catfish pond sediment (Factor N). Each factor was tested at three treatment levels, resulting in nine treatment combinations. Each combination was replicated three times, along with three replications for a negative control, totaling 30 experimental units. Data analysis was conducted using ANOVA, followed by post-hoc testing with the Least Significant Difference (LSD) method at a 5% significance level. The findings indicated that the optimal results were achieved with the N3M3 treatment combination, which involved a dose of 300 ml of fermented rice LOF and 300 ml of catfish pond sediment LOF per liter of water. This combination yielded the best performance in terms of plant height, leaf count, fresh weight, and dry weight.
MEMAKSIMALKAN POTENSI DESA WISATA HALAL KUNJIR LAMPUNG MELALUI PEMBERDAYAAN MASYARAKAT Widiani, Nurhaida; Winandari, Ovi Prasetya
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 5 (2025): Oktober
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i5.34258

Abstract

Abstrak: Pariwisata halal merupakan salah satu sektor strategis yang berperan dalam meningkatkan daya saing daerah sekaligus mendorong kesejahteraan masyarakat. Desa Kunjir, Lampung Selatan, memiliki potensi besar untuk dikembangkan sebagai desa wisata halal, namun masih menghadapi kendala berupa rendahnya pemahaman masyarakat dan pelaku UMKM terkait sertifikasi halal. Kegiatan pengabdian ini bertujuan untuk meningkatkan soft skill (pemahaman pentingnya sertifikasi halal, kesadaran akan standar syariah dalam wisata halal) sekaligus hard skill (keterampilan teknis pendaftaran sertifikasi halal melalui aplikasi SiHalal) bagi pelaku UMKM Desa Kunjir. Metode kegiatan dilakukan dengan pendekatan Asset-Based Community Development (ABCD) melalui tahapan Pra-kegiatan (Discovery), tahapan pelaksanaan (Dream,Design, Delivery), Tahap evaluasi (Destiny). Kegiatan ini diikuti oleh 48 peserta dari 14 UMKM dengan produk unggulan lokal. Evaluasi keberhasilan kegiatan dilakukan melalui wawancara dan analisis data capaian keterampilan mitra. Hasil evaluasi menunjukkan adanya peningkatan signifikan keterampilan peserta: pemahaman pentingnya sertifikasi halal meningkat dari 35% menjadi 90%, pengetahuan prosedur pendaftaran meningkat dari 25% menjadi 85%, keterampilan teknis penggunaan aplikasi SiHalal meningkat dari 10% menjadi 75%, dan sebanyak 14 UMKM berhasil mendaftarkan 15 produk untuk sertifikasi halal (100%). Dengan demikian, kegiatan ini membuktikan bahwa pengembangan desa wisata halal dapat dilakukan secara inklusif dan berkelanjutan melalui penguatan kapasitas UMKM. Desa Kunjir berpotensi menjadi model replikasi bagi desa lain dalam pengembangan wisata halal berbasis pemberdayaan masyarakat.Abstract: Halal tourism is one of the strategic sectors that plays an important role in enhancing regional competitiveness while promoting community welfare. Kunjir Village, South Lampung, has great potential to be developed as a halal tourism village; however, it still faces challenges such as the low level of understanding among the community and MSME actors regarding halal certification. This community service program aims to improve both soft skills (understanding the importance of halal certification and awareness of sharia standards in halal tourism) and hard skills (technical skills in registering halal certification through the SiHalal application) of MSME actors in Kunjir Village. The program was implemented using the Asset-Based Community Development (ABCD) approach through three stages: the pre-activity stage (Discovery), the implementation stage (Dream, Design, Delivery), and the evaluation stage (Destiny). The program involved 48 participants from 14 MSMEs producing local superior products. Evaluation of the program’s effectiveness was conducted through interviews and analysis of partners’ skill achievement data. The results showed a significant increase in participants’ skills: understanding of the importance of halal certification improved from 35% to 90%, knowledge of the registration procedure increased from 25% to 85%, technical skills in using the SiHalal application improved from 10% to 75%, and 14 MSMEs successfully registered 15 products for halal certification (100%). These findings demonstrate that the development of halal tourism villages can be carried out inclusively and sustainably through strengthening the capacity of MSMEs. Kunjir Village has the potential to serve as a replication model for other villages in developing halal tourism based on community empowerment.
Microstructural Mechanisms of Sugarcane Bagasse Biodegradation by Locally Isolated Tropical Cellulolytic Fungi for Environmentally Friendly Composting Nurhaida Widiani; Ovi Prasetya Winandari
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.279-283

Abstract

Sugarcane bagasse is a highly recalcitrant lignocellulosic residue that decomposes slowly under natural conditions, requiring an effective biological agent to accelerate composting. This study evaluated the effectiveness of locally isolated tropical cellulolytic fungi in enhancing bagasse biodegradation based on macromorphological changes and microstructural evidence obtained through Scanning Electron Microscopy (SEM). Four treatments were tested: uninoculated control (P0), single inoculum A (P1), single inoculum B (P2), and consortium A+B (P3), with composting conducted for 56 days under a modified Takakura system. The consortium treatment (P3) exhibited the most advanced compost maturity, characterized by darker coloration, brittle texture, granular structure, and a distinct earthy odor. SEM micrographs further confirmed the greatest extent of lignocellulose breakdown in P3, showing deep longitudinal fissures, fibril delamination, lumen collapse, high porosity, and dense hyphal and spore colonization. These findings indicate that the consortium of cellulolytic fungi accelerates lignocellulose depolymerization through synergistic extracellular enzymatic activity, leading to faster decomposition than single isolates. Overall, the results highlight the bioconversion potential of indigenous tropical fungal consortia as environmentally friendly bioactivators for sustainable management of sugarcane bagasse and other lignocellulosic wastes.