Article Per Year (5 Year)
p-Index From 2021 - 2026
3.93
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Journal of Food and Pharmaceutical Science Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Mutiara Medika: Jurnal Kedokteran dan Kesehatan Majalah Obat Tradisional Jurnal Farmasi Sains dan Komunitas Acta Pharmaciae Indonesia : Acta Pharm Indo BERDIKARI : Jurnal Inovasi dan Penerapan Ipteks Acta Biochimica Indonesiana Menara Perkebunan Journal of Fundamental and Applied Pharmaceutical Science Universitas Muhammadiyah Yogyakarta Undergraduate Conference Proceeding Prosiding Seminar Nasional Program Pengabdian Masyarakat Pharmacy Reports Pharmaceutical Sciences and Research (PSR) Jurnal Farmasi Sains dan Praktis Journal of Food and Pharmaceutical Sciences
Rifki Febriansah
School Of Pharmacy, Faculty Of Medicine And Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta 55183, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

Published : 36 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
Antioxidant, Cytotoxic Activity and Protein Target Inhibition of Ethyl Acetate Fraction Melinjo Seed (Gnetum gnemon L.) by In Vitro and In Silico Studies on HeLa Cervical Cancer Cells Rawi Ingra Savitri; Nuha Haifa Arifin; Rifki Febriansah
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.864-873

Abstract

Cervical cancer is one of the most common cancer suffered in women. Chemotherapy usage often causes physical and psychological side effects in patients. This study aims to determine the antioxidant and cytotoxic effects of the ethyl acetate fraction of melinjo seeds (Gnetum gnemon L.) on HeLa cervical cancer cells through in vitro and in silico assays. Melinjo seed was extracted by maceration using ethanol 70% and fractionated with ethyl acetate to obtain the Ethyl Acetate Fraction of Melinjo Seed (EAFMS). The identification of the active compounds group was done using Thin Layer Chromatography (TLC) method. In vitro studies were conducted on antioxidant tests using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and cytotoxic activity test using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) Assay. In silico test for molecular docking analyzed by Autodock Vina method. The TLC analysis of EAFMS showed the presence of the stilbenoid compounds group. The antioxidant activity of EAFMS is weak, with an IC50 value of 175.8 g/ml. Cytotoxic activity of EAFMS is categorized as toxic to HeLa cancer cells with an IC50 value of 21.69 g/ml, while EAFMS has a synergistic effect combined with doxorubicin as a standard drug with a combination index (CI) value of 0.24-0.80. A molecular docking test of gnetin C with VHR receptor found a strong and stable bond with a docking score of -8.3 kcal/mol. Thus, EAFMS has the potential to be used as a chemopreventive agent for cervical cancer and can be combined with doxorubicin.
Co-Authors Adi Hermawansyah Aditya Fitriasari Aini, Dita Aji Winanta Aji Winanta Aji Winanta Alamsyah, Maura Shavira Aldi Ihza Mahendra Amaliyah, Alfiah Anggi Sabaya Anggraini, Chaessy Yori Arifin, Nuha Haifa Arsito, Puguh Novi Asti Widuri, Asti Cika Fathunissa Desmayanti, Astri Desy Bintang, Desy Devanola Pitaloka Aninda Matsna Aziza Devi Rahmawati, Devi Dita Prabaningrum, Dita Dita Sozianty Dwi Susilo Hardika, Dwi Susilo Dyaningtyas Dewi P. Putri Dzilqi Bustanul Hadi, Dzilqi Bustanul Ega Hida Prabowo Endah Puspitasari Fajriyah, Qatrunada Fatma Sari Masitha Hanik, Linta Sabila Hardi Astuti Witasari Hari Widada Hari Widada, Hari Hidayaturahmah, Rizky Ikfini, Hayu Iwan Dewanto, Iwan Johan Agung Dermawan Julita, Irna Juniananda, Melisa Kenyori, Ivana Kinanti Kintoko Kintoko Krisridwany, Annisa Kusnindyasita, Anisa Kusumaningtyas, Triana Arum Masitha, Fatma Sari Muhammad Arif Rizqi Muhammad Salman Fareza Muhtadi Muhtadi Mulyanti, Amelia Nur'afni Mustafid Amna Nazariah Putri Ningrum, Dhecella Ningrum, Dhecella Winy Cintya Ningsih, Tria Nisriina Yusan Nindy Azzahra Novitasari, Putri Rachma Noviyanti, Vidia Nuha Haifa Arifin Nuha Haifa ARIFIN Nur Ismiyati Nur Oktafiyani, Nur Octavia, Melany Ayu Ramdhani Saifulloh Ranti, Imaniar Rawi Ingra Savitri Sabtanti Harimurti, Sabtanti Saputri, Dwi Saputri, Ginanjar Zukhruf Sarmoko Sarmoko Setiadi, Prismo Setiadi, Prismo Bagas Setiyawati, Fitri Indah Siska Febdian Nitami Siti Nahdiyatul Ummah Sri Tasminatun Totok Suwanda Triana Kusumaningtyas, Triana Utami, Febri Widyaningrum, Rachmawati Yulianto Yulianto