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MCF-7 Resistant Doxorubicin are Characterized by Lamelapodia, Strong Adhesion on Substrate and P-gp Overexpression Putri, Dyaningtyas Dewi P.; Sarmoko, .; Febriansah, Rifki; Puspitasari, Endah; Ismiyati, Nur; Fitriasari, Aditya
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

The Â prognosis Â of Â breast Â cancer Â patients Â is Â closely Â associated Â with Â the Â response Â ofÂ tumor Â cells Â to Â chemotherapy Â agent. Â Doxorubicin Â is Â one Â of Â the Â primary Â chemotherapeuticÂ agents Â used Â for Â the Â treatment Â of Â breast Â cancer. Â Resistance Â to Â chemotherapy Â is Â believed Â toÂ cause Â treatment Â failure Â in Â cancer Â patients. Â Furthermore, Â long Â time Â exposure Â toÂ chemotherapeutic Â agent Â induces Â cancer Â cells Â resistance. Â MCF-7 Â sensitive Â cells Â used Â asÂ chemoresistance Â model Â have Â overexpression Â P-gp Â (P-glycoprotein). Â Chemoresistance Â wasÂ established by treating MCF-7 cells with 0.5 Âµg/ml doxorubicin-contained medium for a week.Â 50% inhibiting concentration (IC50) doxorubicin on MCF-7 cells/DOX were determined usingÂ MTT assay. Western blot assay and immunocytochemistry assay was performed to determineÂ the expression of P-gp. Morphological of MCF-7 cell/DOX was changing to become larger andÂ have Â lamellapodia. Â IC50 Â value Â of Â doxorubicin Â was Â 700 Â nM Â on Â MCF-7/DOX Â and Â 400 Â nM Â onÂ sensitive MCF-7 cells. The MCF-7/DOX sensitivity to doxorubicin was decreased, shown byÂ 1.5 Â fold Â higher Â IC50 Â of Â doxorubicin Â on Â MCF-7/DOX Â compared Â to Â MCF-7 Â sensitive Â cells.Â Treatment doxorubicin to sensitive MCF-7 cells leads to the increasing P-gp expression. TheÂ P-gp Â level Â expression Â has Â strong Â correlation Â with Â the Â low Â sensitivity Â of Â MCF-7/DOX Â toÂ doxorubicin.Key words: doxorubicin, resistance cells, sensitive MCF-7 cell

Antioxidant Activity from the Combination of Ethanolic Extract of Tea Leaves (Camellia sinensis) and Soursop Leaves (Annona muricata l.) and Optimation of the Effervescent Granule Production Masitha, Fatma Sari; Febriansah, Rifki
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 19, No 2: July 2019
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mm.190230

Soursop Leaves (Annona muricata L.) and Tea Leaves (Camellia sinensis) are plants that contain flavonoids which are considered to act as antioxidants and can become chemopreventive agents. This study aims to identify the potential of Soursop Leaves and Tea Leaves as chemopreventive agents. The first stage of this research was to determine the presence of flavonoids in both of ethanolic extract using TLC method. The second stage is to examine the antioxidant activity of the combination of ethanolic extract of soursop leaves and tea leaves against free radical DPPH (1,1-diphenyl-2-pikrihidrazil). Docking molecular test of acetogenin and catechin compounds at HER-2 and ER-Î± receptors which are biomarkers of breast cancer. Furthermore, the optimization of effervescent granule production using moist granulation method is carried out. The result shows that ethanolic extract of soursop leaves and tea leaves contains flavonoid shown with Rf value of 0.66 and 0.68, and the comparison of Rf 0.66. The combination of both ethanolic extract has very strong antioxidant activity with IC50 value 26,90 Î¼g/mL. The result of molecular docking of acetogenin and catechin compounds at HER-2 receptors shows respective docking score of -6.3 kcal/mol and -6.7 kcal/mol while ER-Î± receptors shows a docking score of -6.5 kcal/mol and -7.6 kcal/mol. In addition, the most optimal effervescent granule production is formula four with physical test result such as moisture content of 1.07% with a dissolve time of 45.5 seconds and acidity of 6.18. The result shows that ethanolic extract of soursop leaf (Annona muricata L.) and Tea Leaves (Camellia sinensis) have potential activity as chemopreventive agents and can be formulated into effervescent granule production.

Kajian secara In Vitro Ekstrak Etanolik Buah Morinda citrifolia L. sebagai Agen Khemopreventif Kanker Payudara yang Potensial Febriansah, Rifki; Bintang, Desy; Hardika, Dwi Susilo; Prabaningrum, Dita; Hadi, Dzilqi Bustanul; Oktafiyani, Nur
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 12, No 3 (2012)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v12i3.1038

Kanker payudara merupakan kanker terbanyak di Indonesia yang menyebabkan kematian sampai 4,3 juta orang per tahun. Sampai saat ini pengobatan yang biasa dijalani oleh penderita kanker payudara adalah dengan melakukan kemoterapi, tetapi karena efek sampingnya yang relatif besar, banyak pengobatan alternatif mulai dikembangkan. Pengobatan menggunakan bahan herbal menjadi populer untuk menggantikan pengobatan kimiawi tidak hanya karena memiliki efek yang serupa, tetapi juga karena keamanannya. Salah satu agen khemopreventif dari bahan alam adalah menggunakan buah mengkudu (Morinda citrifolia L.). Tujuan penelitian ini adalah untuk mengetahui efek ekstrak etanolik buah mengkudu dalam menghambat pertumbuhan sel kanker payudara melalui uji sitotoksik dan uji apoptosis. Sebanyak 300 gram serbuk mengkudu diekstraksi dengan 2 Liter etanol kemudian diujikan terhadap sel kanker payudara MCF-7. Uji sitotoksik dilakukan dengan metode MTT untuk mendapatkan nilai IC50. Uji Induksi Apoptosis dilakukan dengan metode double staining menggunakan reagen etidium bromida-akridin oranye. Hasil penelitian menunjukkan nilai IC50 adalah sebesar 1117 Â¼g/ml dan dari hasil pengamatan uji apoptosis menunjukkan adanya kenaikan apoptosis sel dengan peningkatan dosis pemberian ekstrak etanolik. Disimpulkan bahwa ekstrak etanolik buah mengkudu berpotensi sebagai agen kemopreventif melalui mekanisme induksi apoptosis pada sel MCF-7.Â The incidence of breast cancer is the biggest one that causing a huge number of deadly in Indonesia until 4,3 million per years. Until this time, the common medication to threat the cancer by chemotherapy still popular, but because of the negative effect, many alternative herbal medicine was developed faster not only because the safety but also the effectivity to treat. One of the natural substance which can be used as chemopreventive agent is fruits Morinda citrifolia L. The purpose of the research is to know the effects of noniâ€™s extractÂ as chemopreventive agent for breast cancer by inhibitory cell proliferation and the ability for apoptotic induction. Three hundred grams noniâ€™s flour extracted with 2 liters 70 % ethanol then tested to MCF-7 breast cancer cell lines. Cytotoxic test was done by MTT assay method to get IC50 value. Apoptotic assay was analyzed by double staining method used ethidium bromide-acrydine orange reagent. The result showed that IC50 value of noniâ€™s extract was 1117 mg/ml and the result of apoptotic assay showed that in higher concentration of ethanolic extract could increased apoptotic induction. The conclusion of the research is noniâ€™s etanolic extract having potency as chemopreventive agent by increased apoptotic induction of MCF-7 cell lines.

Pemberdayaan Kelompok Tanaman Obat Keluarga Menuju Keluarga Sehat Di Desa Sumberadi, Mlati, Sleman Febriansah, Rifki
Berdikari: Jurnal Inovasi dan Penerapan Ipteks Vol 5, No 2 (2017): August
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/bdr.5221

Pengembangan tanaman berkhasiat obat telah mengalami percepatan hingga pada penemuan obat maupun teknologi baru. Teknologi terapan harus dapat diimplementasikan agar mendatangkan manfaat luas hingga lapisan terbawah melalui kelompok-kelompok masyarakat. Kelompok tani dan masyarakat pedesaan memiliki peran vital dalam pembangunan masyarakat, tidak hanya dalam kemandirian pangan, namun juga dapat diarahkan pada kemandirian kesehatan melalui pengembangan tanaman obat keluarga. Kelompok Tanaman Obat Keluarga (TOGA) di Dusun Warak Kidul dan Dusun Gabahan VI adalah kelompok tani rintisan di Desa Sumberadi, Kecamatan Mlati, Kabupaten Sleman, wilayah yang dekat dengan perkotaan namun kondisi masyarakatnya masih membutuhkan pembinaan. TOGA identik dengan jamu yang berasal dari tanaman obat yang berasa pahit, tidak memiliki nilai estetika dan tidak enak dikonsumsi, sehingga pengembangannya masih terbatas karena kurang diminati. Masyarakat belum menyadari bahwa sayuran dan bumbu dapur juga merupakan herbal berpotensi obat, sehingga dapat dikategorikan sebagai TOGA. Pengabdian masyarakat ini bertujuan untuk meningkatkan pengetahuan dan kemampuan warga masyarakat khususnya anggota kelompok TOGA dalam pemanfaatan dan pengolahan tanaman obat menjadi bentuk sediaan yang lebih ekonomis. Kegiatan pengabdian masyarakat dimulai dengan pembentukan kelompok TOGA yang beranggotakan para ibu rumah tangga di dusun tersebut. Selanjutnya dilakukan penyuluhan tentang khasiat tanaman obat, pembuatan kebun TOGA yang benar, proses pembuatan obat herbal yang tepat dan pembentukan pos herbal desa sebagai fasilitator dan pendamping para anggotanya. Dari kegiatan pengabdian masyarakat ini dapat disimpulkan bahwa pengetahuan masyarakat meningkat terkait pemanfaatan tanaman obat dan dapat meningkatkan taraf perekonomian khususnya para anggota kelompok TOGA di Desa Sumberadi.Â Kata Kunci : Desa Sumberadi, kebun TOGA, penyuluhan, sediaan obat herbal, pos herbal desa

Pemberdayaan Kelompok Tanaman Obat Keluarga Menuju Keluarga Sehat Di Desa Sumberadi, Mlati, Sleman Febriansah, Rifki
Berdikari: Jurnal Inovasi dan Penerapan Ipteks Vol 5, No 2 (2017): August
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/bdr.5221

Pengembangan tanaman berkhasiat obat telah mengalami percepatan hingga pada penemuan obat maupun teknologi baru. Teknologi terapan harus dapat diimplementasikan agar mendatangkan manfaat luas hingga lapisan terbawah melalui kelompok-kelompok masyarakat. Kelompok tani dan masyarakat pedesaan memiliki peran vital dalam pembangunan masyarakat, tidak hanya dalam kemandirian pangan, namun juga dapat diarahkan pada kemandirian kesehatan melalui pengembangan tanaman obat keluarga. Kelompok Tanaman Obat Keluarga (TOGA) di Dusun Warak Kidul dan Dusun Gabahan VI adalah kelompok tani rintisan di Desa Sumberadi, Kecamatan Mlati, Kabupaten Sleman, wilayah yang dekat dengan perkotaan namun kondisi masyarakatnya masih membutuhkan pembinaan. TOGA identik dengan jamu yang berasal dari tanaman obat yang berasa pahit, tidak memiliki nilai estetika dan tidak enak dikonsumsi, sehingga pengembangannya masih terbatas karena kurang diminati. Masyarakat belum menyadari bahwa sayuran dan bumbu dapur juga merupakan herbal berpotensi obat, sehingga dapat dikategorikan sebagai TOGA. Pengabdian masyarakat ini bertujuan untuk meningkatkan pengetahuan dan kemampuan warga masyarakat khususnya anggota kelompok TOGA dalam pemanfaatan dan pengolahan tanaman obat menjadi bentuk sediaan yang lebih ekonomis. Kegiatan pengabdian masyarakat dimulai dengan pembentukan kelompok TOGA yang beranggotakan para ibu rumah tangga di dusun tersebut. Selanjutnya dilakukan penyuluhan tentang khasiat tanaman obat, pembuatan kebun TOGA yang benar, proses pembuatan obat herbal yang tepat dan pembentukan pos herbal desa sebagai fasilitator dan pendamping para anggotanya. Dari kegiatan pengabdian masyarakat ini dapat disimpulkan bahwa pengetahuan masyarakat meningkat terkait pemanfaatan tanaman obat dan dapat meningkatkan taraf perekonomian khususnya para anggota kelompok TOGA di Desa Sumberadi.Â Kata Kunci : Desa Sumberadi, kebun TOGA, penyuluhan, sediaan obat herbal, pos herbal desa

Potency Of Andrographolide, L-Mimosine And Asiaticoside Compound As Antiviral For Covid-19 Based On In Silico Method Ningrum, Dhecella; Aini, Dita; Juniananda, Melisa; Febriansah, Rifki
Proceedings of Universitas Muhammadiyah Yogyakarta Graduate Conference Vol. 2 No. 2 (2023): Strengthening Youth Potential for Sustainable Innovation
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/umygrace.v2i2.418

Introduction - COVID-19 is an infectious disease that is a global challenge, making it necessary for innovation and the development of antiviral agents in prevention and treatment efforts. Indonesia is known as a country rich in potential plants as medicine, a potential antiviral plant among them Andrographis paniculata , Mimosa pudica and Centella asiatica. The compound that have antiviral activity in each of these plants are andrographolide (Andrographis paniculata), L-mimosine (Mimosa pudica) and asiaticoside (Centella asiatica). The purpose of this study is to identify the potential of the andrographolide, l-mimosine and asiaticoside to inhibit SARS COV-2 protein and to predict the molecular drug profile which refers to lipinski's rule of 5. Purpose - The purpose of this study was to determine the potential of Andrographis paniculata , Mimosa pudica and Centella asiatica compounds in inhibiting the SARS CoV-2 protein and to determine the prediction of drug profiles such as molecules referring to Lipinski's rule of 5. Methodology - The methods used in this research by molecular docking and lipinski's Ro5 using PKCSM. The control compounds used are favipiravir and remdesivir. Results - The results of the affinity of target protein bonds using molecular docking obtained the best results in NSP3 proteins with An Asiaticoside docking score of -10.1 kcal / mol stronger than favipiravir -5.3 kcal/mol and remdesivir -8.6 kcal/mol. As for the compounds L-mimosine -6.1 kcal / mol and Andrographolide -7.7 kcal / mol. The compounds of andrographolide and L-mimosine showed good results and met the 5 aspects of Lipinski's Ro5, but asiaticoside compounds did not good enough. . Conclusion - The Research has concluded that the compound of the Andrographis paniculata , Mimosa pudica and Centella asiatica have a good potential in inhibiting SARS cov2 protein that is stronger than favipiravir and almost the same with remdesivir activity.

Investigation of Moringa Leaf Compounds as Colon Anticancer Agents Using Bioinformatics and Molecular Docking Methods Saputri, Dwi; Kusumaningtyas, Triana; Setiadi, Prismo; Febriansah, Rifki
Proceedings of Universitas Muhammadiyah Yogyakarta Graduate Conference Vol. 2 No. 2 (2023): Strengthening Youth Potential for Sustainable Innovation
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/umygrace.v2i2.421

Introduction - Colon cancer is a disease that has a high mortality rate. Cancer treatments such as chemotherapy can cause severe side effects. So, it is necessary to do research on herbal plants to minimize these side effects. The bioactive compounds in Moringa leaves (Moringa oleifera L.) are known to have antioxidant and anticancer activity, so they have the potential to be used as cancer treatment. Purpose – . This study aims to determine the potential of Moringa leaves as an anticancer agent in silico. Methodology/Approach – The in silico test used is STITCH-STRING bioinformatics and molecular docking. STITCH-STRING bioinformatics was used to determine the target protein of the test compound, and molecular docking was used to determine the binding affinity between the test compound and cancer protein. Findings – In the STITCH-STRING bioinformatics test, the potential targets for kaempferol and quercetin were TP53-ESR1 and STAT3-EGFR, respectively.test Molecular docking on cancer protein showed that quercetin compound in EGFR protein had the best binding with a docking score of -9.2 kcal/mol. This shows good results because the docking score obtained is lower than the comparison drug (Fluorouracil) which has a docking score of -4.2 kcal/mol. These results indicate that compounds in Moringa leaves (Moringa oleifera L.) have the potential to be used as anticancer agents. Originality/ Value/ Implication – Moringa leaf research using bioinformatics and molecular docking methods is still rarely done, so this research can be used as further knowledge related to the search for moringa leaf compounds that have the potential for anticancer using bioinformatics and molecular docking methods

Screening the Activity of Epigallocatechin Gallate as a Colorectal Anticancer Agent: In Silico and ADMET/Pharmacokinetic Studies Setiadi, Prismo Bagas; Alamsyah, Maura Shavira; Ningsih, Tria; Fajriyah, Qatrunada; Febriansah, Rifki
Proceedings of Universitas Muhammadiyah Yogyakarta Graduate Conference Vol. 3 No. 2 (2024): Crafting Innovation for Global Benefit
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/umygrace.v3i2.602

Introduction - Colorectal cancer is one of the third ranked cancers in Indonesia and has a high level of malignancy. The bioactive compound Epigallocatechin Gallate (EGCG) is known to have antioxidant and cytotoxic activity, so it has the potential to be developed as an anticancer agent. Purpose - This study aims to determine the potential of EGCG compounds as anticancer in silico. Methodology/Approach - The in-silico tests used are molecular docking and ADMET/Pharmacokinetic profiles using biosig pkcsm.Finding - The results of the respective binding affinities formed from EGCG compounds with the target protein affinities of COX-2 and Caspace-3 are -9.2 and -5.4 which are better links between EGCG and Caspace. The Lipinski rule show that the epigallocatecin compound has strength to absorb is 47%. This indicates that EGCG has the potential as a candidate for colorectal anticancer drugs with poor absorption. Originality/Value/Implications – In Silico, many studies have been carried out, but it is still rare to combine molecular docking methods with pKCSM to determine the solubility properties of compounds. The prospect of this research can become a basic or key for developing ECGC compounds into preparations because they have low bioavailability and can be continued in in vitro and in vivo research.

Exploration of date palm (Phoenix dactylifera) bioactivity as anti-SARS-CoV-2: in silico study Arifin, Nuha Haifa; Febriansah, Rifki
Pharmacy Reports Vol. 2 No. 3 (2022): Pharmacy Reports
Publisher : Indonesian Young Scientist Group and UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51511/pr.50

The COVID-19 pandemic has highlighted the need for innovation and the development of antiviral agents. One plant with potential antiviral properties is the date palm (Phoenix dactylifera). However, research on the antiviral activity of dates against SARS-CoV-2 is limited. This study aims to assess the antiviral potential of compounds found in date palm fruit using a molecular docking method. Specifically, we compare these compounds to the antiviral drugs ritonavir and nirmatrelvir in their ability to inhibit SARS-CoV-2 proteins. The molecular docking analysis was conducted using various tools and software, including Autodock Vina, DS Visualizer, Autodock Tools, Python, and Marvin Sketch. The results of the study indicate that compounds such as apigenin, diosmetin, and luteolin have strong potential as antiviral agents. The binding affinity of apigenin in date fruit with various SARS-CoV-2 proteins is as follows: -7.6 kcal/mol for 3CL-Pro, -8.7 kcal/mol for Nsp3, -5.7 kcal/mol for PD-ACE-2, and -7.0 kcal/mol for RBD-S. Diosmetin exhibits similar binding affinities with these proteins: -6.7 kcal/mol, -8.5 kcal/mol, -5.6 kcal/mol, and -7.2 kcal/mol, respectively. Luteolin also shows strong binding affinities: -7.9 kcal/mol, -8.6 kcal/mol, -5.7 kcal/mol, and -7.3 kcal/mol. In comparison, nirmatrelvir achieved docking scores of -7.2 kcal/mol, -7.5 kcal/mol, -5.1 kcal/mol, and -6.3 kcal/mol with the same proteins, while ritonavir scored -7.0 kcal/mol, -8.2 kcal/mol, -5.6 kcal/mol, and -6.7 kcal/mol, respectively. Apigenin, diosmetin, and luteolin demonstrate stronger potential than nirmatrelvir and ritonavir, as evidenced by their lower docking scores when compared to these drugs.

Anticancer Activity of The Ethanol Fraction of Gnetum gnemon L. Seeds on HeLa Cell Lines Rahmawati, Devi; Ningrum, Dhecella Winy Cintya; Kenyori, Ivana Kinanti; Febriansah, Rifki; Octavia, Melany Ayu; Hermawansyah, Adi
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 23, No 2 (2023): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v23i2.18421

Cervical cancer results from abnormal cell development in the cervix and is caused by the Human Papillomavirus (HPV). Cancer treatment with chemotherapy drugs has dangerous side effects because it can attack normal cells. Gnetum gnemon L. seeds are known to possess anticancer potential. Therefore, natural ingredients are necessary to develop new cancer drug strategies with minimal side effects. This study aims to determine the anticancer activity of the ethanol fraction of Gnetum gnemon L. Seeds (EFGS) against HeLa cells. The research method used in this study was an identification test to detect compounds from EFGS using the TLC method and the HeLa cell cytotoxic test using the MTT Assay method. The results showed that EFGS contained stilbenoid group compounds based on TLC-Densitometry, with an Rf value of 0.22 cm. The cytotoxic test results on HeLa cells indicated an IC50 value of 784 µg/mL, classifying it as having the potential as a moderate category anticancer agent. Therefore, this study indicates that EFGS can be utilized as a chemopreventive agent.

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