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All Journal Bioscience Bioeducation Journal Pendidikan Biologi Jurnal Pembangunan dan Alam Lestari SEMIRATA 2015 BIO-SITE |BIOLOGI Sains Terapan Eksakta : Berkala Ilmiah Bidang MIPA Atrium Pendidikan Biologi Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi BERNAS: Jurnal Pengabdian Kepada Masyarakat BIOCHEPHY: Journal of Science Education Jurnal Serambi Biologi
Mades Fifendy
Universitas Negeri Padang
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Journal : Jurnal Serambi Biologi

 1 
Effect of Papaya Leaf Extract (Carica papaya L.) on Colony Diameter and Percentage of Growth Inhibition of Fusarium oxysporum Meriza Fatma; Moralita Chatri; Mades Fifendy; Dezi Handayani
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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This study aimed to determine the effect of leaf extract concentration of C. papaya on the diameter of colonies F. oxysporum and the percentage of inhibition of growth of F. oxysporum. This research was conducted from February to April 2021 at the Integrated Research Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study was an experimental study consisting of 5 treatments and 3 replications with the administration ofleaf extract at a C. papaya concentration of 0% (control), 10%, 20%, 30% and 40%. The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (further testDNMRT)and the percentage of inhibition was analyzed descriptively. The results showed that leaf extract C. papaya at concentrations of 20%, 30% and 40% significantly affected the diameter of thecolonies F. oxysporum. The smallest colony diameter was at a concentration of 40% was 5.35 cm and the largest colony at a concentration of 10% was 6.61 cm. While the smallest percentage of inhibition at a concentration of 10% was 5% and the largest percentage of inhibition is at a concentration of 40% was 23%.
Test The Inhibition of Beringin Leaf Extract (Ficus benjamina L.) Against The Growth of Candida albicans in Vitro Santi Rodiah; Mades Fifendy; Gustina Indriati
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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This study aimed to determine the inhibitory ability of F. benjamina leaf extract on the growth of the fungus C. albicans and the optimum concentration in inhibiting the growth of the fungus C. albicans. This research was conducted from October to December 2021 at the Microbiology Laboratory and Research Laboratory of the Department of Biology, Faculty of Mathematics and Science, Padang State University. This study is an experimental study consisting of 7 treatments and 3 repetitions with F. benjamina leaf extract at concentrations of 30%, 40%, 50%, 60%, 70% as well as negative control (sterile distilled water) and positive control (10% ketoconazole). Data for the diameter of the inhibition zone was analyzed by means of variance (ANOVA) and continued with further tests Duncan's Multiple Range Test (DMRT) further test. The results showed that the leaf extract of F. benjamina was able to inhibit the growth of the fungus C. albicans. The optimum concentration of F. benjamina leaf extract in inhibiting the growth of the fungus C. albicans at a concentration of 60% was 18.91 mm.
THE EFFECT OF VARIOUS CONCENTRATIONS OF ANTI-ACNE LIQUID SOAP ON THE BACTERIA OF Staphylococcus aureus CAUSES ACNE Delfi Suganda; Mades Fifendy; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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his study aims to determine the effect of various concentrations of anti-acne liquid soap on Staphylococcus aureus and the interaction of the two types of liquid soap with their concentrations on the growth of S. aureus bacteria that causes acne. This research is an experimental study using a completely randomized design (CRD) in a 6 x 2 factorial with 3 replications. Factor A is liquid soap for acne, 2 levels are A1 = (Initial: P) , A2 = (Initial: C&C) Factor B is soap concentration, 6 levels are B1 = 2.5% (w/v), B2 = 5 % (w/v), B3 = 7.5% (w/v), B4 = 10% (w/v), B5 = 12.5% ​​(w/v), B6 ​​= Control (sterile distilled water). The results of this study indicate that the effect of various concentrations of anti-acne liquid soap on Staphylococcus aureus bacteria that causes acne is able to inhibit the growth of S. aureus, because the C&C brand of liquid anti-acne soap is able to form an average inhibition zone diameter of 1.94 cm at a concentration of 12, 5% while the anti acne P liquid soap brand was able to form an average inhibition zone diameter of 1.64 cm. From the statistical analysis data using the ANOVA test, the effect of various anti-acne liquid soaps on S.aureus had no significant effect and there was no interaction between the types of anti-acne liquid soap and its concentration on S.aureus which causes acne. Key words anti acne liquid soap, Staphylococcus aureus, concentration
Anti acne Solid Soap Antimicrobial Activity Test against Staphylococcus aureus Bacteria that Causes Acne Ade Indra Utama; Mades Fifendy; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The purpose of this study was to determine the antimicrobial activity of the anti-acne solid soap type and the effect of its concentration as well as the interaction between the anti-acne solid soap type and its concentration against Staphylococcus aureus bacteria that causes acne. This research was conducted from October - December 2021 at the Microbiology Laboratory, Faculty of Mathematics and Science, Universitas Negeri Padang. The methodology used in this study was an experiment with disc diffusion method using a completely randomized design (CRD) in a factorial consisting of 6 treatments and 3 repetitions with anti acne solid soap at concentrations of 2.5%, 5%, 7.5%, 10 %, 12.5% (w/v) and control (sterile distilled water). The inhibition zone diameter data were statistically analyzed using ANOVA, with = 5% and continued with Duncan's New Multiple Range Test (DNMRT) further test. The results of this study showed that both anti-acne solid soaps were able to provide antimicrobial activity against S. aureus bacteria at different concentrations because brand A and J solid anti-acne soap were able to form the highest average diameter of inhibition zones of 2.67 cm and 4, respectively. ,39 cm at 5% concentration. From the statistical analysis data using ANOVA, it was seen that the type and concentration of anti-acne solid soap had no significant effect on S. aureus bacteria and there was no interaction between the type of anti-acne solid soap and its concentration on S. aureus bacteria that causes acne.
Effect of Sungkai Leaf Extract (Peronema canescens J.) on Colony Diameter and Percentage of Growth of Inhibition Fusarium oxysporum Marisa Marisa; Moralita Chatri; Linda Advinda; Mades Fifendy
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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The purpose of this study was to determine the effect of the concentration of leaf extract P. canescens on colony diameter and the percentage of inhibition of growth of F. oxysporum. This research was conducted from November 2021 to December 2021, at the Integrated Research Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study was an experimental study consisting of 5 treatments and 3 replications with leaf extract at P. canescens concentrations of 0%, 10%, 20%, 30%, 40%. The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (DNMRT) further test. The results showed that the leaf extract of P. canescens . Effect on the diameter of the fungal colonies of F. oxysporum. The concentration of leaf extract affected the percentage of growth of inhibition F.oxysporum. The lowest percentage of inhibition was at a concentration of 10% and the highest at a concentration of 40%.
Resistance Test of Matoa Leaf Extract (Pometia pinnata J.R & G.Forst) On Fungi Growth of Candida albicans In-vitro Intan Rahma Putri; Mades Fifendy; Gustina Indriati
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Fungal infection is one of the most common infectious diseases in the world, including Indonesia. Candidiasis is a dangerous disease, it is proven that the cases of death caused by candidiasis are in the range of 30-40% every year. Candidiasis is an infectious disease caused by fungi of the genus Candida, of which about 70% are caused by the species Candida albicans. Antifungal resistance can become a serious problem in the future. P. pinnata leaves are known to contain secondary metabolites, namely saponins, flavonoids, alkaloids, terpenoids and tannins. The purpose of this study was to determine the inhibition of P. pinnata leaf extract on the growth of the fungus C. albicans. This research was conducted using a completely randomized design (CRD) method with 6 treatments and 3 repetitions. The treatments were control using 10% ketoconazole and P. pinnata leaf extract (30%, 40%, 50%, 60%, 70%). The data that has been obtained were analyzed using ANOVA with a confidence level of = 5% and further tested with DNMRT. The results showed that P. pinnata leaf extract was able to inhibit the growth of the fungus C. albicans at concentrations of 70%, 60%, 50%, 40%, and 30% with an average diameter of the inhibition zones 10,10 mm, 11,62 mm, 12,78 mm, 12,34 mm, 10,97 mm.
Uji Daya Hambat Ekstrak Daun Gambir (Uncaria gambir Roxb.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus Putri Rahma Padilla; Mades Fifendy; Irdawati; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Staphylococcus aureus merupakan salah satu mikroflora normal yang berada dalam mulut, apabila dipengaruhi oleh faktor predisposisi seperti perubahan kuantitas mikroorganisme menjadi tidak seimbang akan menimbulkan infeksi. Beberapa penyakit dalam rongga mulut dan sekitarnya yang dapat disebabkan oleh Staphylococcus aureus yaitu abses, ginggivitis, angular cheilitis, parotitis, staphyloccal mucositis dan denture stomatitis. Untuk mengatasi infeksi Staphylococcus aureus dapat digunakan suatu alternatif, salah satunya mencari senyawa-senyawa yang berpotensi sebagai antibiotik dari tumbuhan yang memiliki khasiat obat. Pengobatan untuk infeksi Staphylococcus aureus adalah dengan pemberian antibiotik yang dapat mengahambat pertumbuhan atau mematikan Staphylococcus aureus yang menginfeksi. Salah satu tanaman yang dapat dimafaatkan sebagai obat tradisional sebagai antibiotik adalah daun gambir (Uncaria gambir). skrining fitokimia dari ekstrak daun gambir menunjukan terdapatnya kandungan golongan senyawa kimia di dalam simplisia dan ekstrak etanol gambir yaitu alkaloid, flavonoid, saponin, dan tannin yang mempunyai sfektivitas sebagai antioksidan. Penelitian ini merupakan penelitian eksperimen, menggunakan metode RAL yang terdiri dari 6 perlakuan dan 3 pengulangan dengan pemberian ekstrak daun gambir konsentrasi 10%, 20%, 30%, 40%, 50% serta kontrol positif (Amoxicilin 10%). Hasil penelitian menunjukkan bahwa ekstrak daun gambir mampu menghambat pertumbuhan bakteri Staphylococcus aureus. Konsentrasi optimum ekstrak daun gambir dalam menghambat pertumbuhan bakteri Staphylococcus aureus pada konsentrasi 50% sebesar 21,6 mm.
Co-Authors Ade Indra Utama Andri Akhiruddin Lubis Anik Aspirin Ardi Ardi Armen Amir Asrul Azwir Anhar Betti Anggrayeni Chairatun Dyna Delfi Suganda Deni Kurniati Des M Des M Desi Musliati Desriyati Desriyati Dezi Handayani Dezi Handayani Diana Susanti Diga Mulya Dila Fitria Dini Yusta Prestu Ningsih Dwi Hilda Putri Eka Vidya Putra Elsa Yuniarti Faradilla Rattriana Fera Mawita Fitri Deswita Gustina Indriati Gustina Indriati Halizah Halizah Heffi Alberida Hendra Lardiman I Ketut Suada Ilham Pratama Intan Rahma Putri Irdawati Irdawati Irdawati Irdawati irma - leilani Irma Lailani Eka Putri Lara Annisa Linda Advinda Mardiah Tajun Marisa Marisa Meriza Fatma Metatia Monica Moca Faulina putri Moralita Chatri Muhyiatul Fadilah Multi Asmul Sovia NAJMI ARIF Nani Subandini Nur Annisah Nur Asiah Siregar Nurmiati Nurmiati Oki Jumaidi Oktavianes Oktavianes Periadnadi Periadnadi Pitri Anita Primayanti Jasdi Pujiana Okta Putri Putri Rahma Padilla Rahmadhani Fitri Rahmawati D Raysa Juwita Relsas Yogica, Relsas Rezi Junialdi Rhini Febrianti Ridhatul Rahayu Lova Ristiono Ristiono Rizki Rizki Rizki Rizki RRP Megahati Siregar RRP Megahati Siregar Santi Rodiah Selaras, Ganda Hijrah Shinta Sari Maria Sisri Muharti Sisri Yandila SUCI FAJRINA Suci Pebra Jusana Syadzli Agus Saputera Syovia Osnita Syukryani Syukryani Vega Ersalina Verawati Yesni Viola Mecita Vivi Fitriani Widya Anggrayesti Wisma Kayani Witria Malra Sari Yossi Rahmadeni Yuni Sepriani Zelvia Misdar