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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pendidikan Matematika dan IPA AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Human Nutrition Biota Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Mangifera Edu BioEksakta : Jurnal Ilmiah Biologi Unsoed Berkala Penelitian Hayati Kaunia: Integration and Interconnection of Islam and Science Journal Kenanga : Journal of Biological sciences and Applied Biology Gunung Djati Conference Series Berkala Ilmiah Biologi Jurnal Kefarmasian Indonesia
Laksmindra Fitria
Universitas Gadjah Mada
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Veterinary Other

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TOKSISITAS ORAL AKUT SINGLE-DOSE EKSTRAK ETANOLIK DAUN GAHARU Aquilaria malaccensis Lamk. PADA TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR Fitria, Laksmindra; Cannadianti, Nadhira; Putri Gunawan, Ismi Cahya
KENANGA : Journal of Biological Sciences and Applied Biology Vol. 4 No. 2 (2024): Oktober 2024
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/kenanga.v4i2.5404

Abstract

Aquilaria malaccensis Lamk., one of tree species producing agarwood, has been utilized by the people of Southeast Asia (particularly Indonesia and Malaysia) as a component in traditional medicine due to their phytochemical constituents. This study was carried out to assess acute toxicity of oral administration of ethanolic extract of A.malaccensis (EEAM) using female Wistar rats as model animal. Procedure in accordance with OECD Guidelines for the Testing of Chemicals Test No. 423 with administration dose of 2000 mg/kg bw (single-dose). Parameters observed including signs of toxicity, body weight, body temperature, hematological profile (complete blood count), as well as evaluation of liver function (ALT and bilirubin) and renal function (BUN and creatinine). Data were analyzed statistically based on one-way ANOVA (ɑ=0.05) continued with Duncan's post hoc test (ɑ=0.05) and visualized as line charts. Results showed that neither mortality nor sublethal effects were recorded during 14 days of the experiment. The chart exhibited that the group received EEAM demonstrated a comparable trend to the control group for all parameters. Therefore, it can be concluded that EEAM is safe or no-observed-adverse-effect-level (NOAEL) for single-dose oral administration at the dose of 2000 mg/kg bw. If standardized following the Globally Harmonized System of Classification and Labeling of Chemicals (GHS), EEAM is classified in Category 5 (LD50 > 2000 mg/kg bw). Based on this finding, we will continue to conduct further study to assess the repeated-dose acute oral toxicity of EEAM (subacute period)
NILAI RUJUKAN UNTUK EVALUASI FUNGSI HATI DAN GINJAL PADA TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR Fitria, Laksmindra; Lukitowati, Fajar; Kristiawati, Dian
Jurnal Pendidikan Matematika dan IPA Vol 10, No 2 (2019): Juli 2019
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1037.481 KB) | DOI: 10.26418/jpmipa.v10i2.34144

Abstract

Evaluation of liver and renal functions are routine tests performed in preclinical studies. Liver plays a role in detoxification and secretion of various substances to regulate a wide range of metabolic functions. Kidneys serves as the main excretory and osmoregulatory organs. Thus, assessment of liver and renal functions are important in identifying the presence of damages and diseases as well as monitoring body responses towards treatments or therapy. Therefore, normal values should be established for valid reference. Until now, many researchers still use references from various publications, including foreign countries with different environmental factors and method of animals care. This study was aimed to provide reference values for liver and renal functions in male and female Wistar rats. Animals were sourced from LPPT-UGM. Age 4-, 6-, and 8-week-old represent juvenile, preadult, and adult. Blood samples were collected from orbital sinus of anesthetized animals. Sera were tested for ALT, AST, BUN, and creatinine based on spectrophotometry. Results demonstrated that values of most parameters varied, depend on sex and age. According to this finding, it can be concluded that reference values cannot be generalized but must be determined by sex and age in local population.
Role of Ficus carica in Alleviating Chronic Hypoxia Impact in Rat’s Lungs Anggraini, Dini; Meliala, Andreanyta; Siswanto; Fitria, Laksmindra; Narwidina, Paramita; Mahroos, Rifda El; Sudesty, Shellya Puti
Indonesian Journal of Human Nutrition Vol. 11 No. 2 (2024)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2024.011.02.3

Abstract

Chronic Intermittent Hypoxia (CIH) can initiate oxidative stress and inflammation. Ficus carica is known to contain antioxidant compounds capable of suppressing oxidative stress. This study aims to investigate the protective role of Ficus carica puree (FCP) on the levels of Malondialdehyde (MDA), Superoxide Dismutase (SOD), SOD/MDA ratio, Platelet Lymphocyte Ratio (PLR), and Tumor Necrosis Factor-α (TNF-α) in the lungs of rats induced by CIH. Rats were treated with FCP at doses of 1.25 ml/200g (CIH-F1), 2.5 ml/200g (CIH-F2), and 5 ml/200g (CIH-F3). All rats, except for the neutral control group, were subjected to chronic intermittent hypoxia (CIH) for 4 hours/day for 30 days. The CIH-F2 and CIH-F3 groups demonstrated significantly lower levels of lung MDA compared to the CIH group. The CIH-F3 group showed a considerably lower level of lung SOD compared to the CIH group. However, the SOD/MDA exhibited a considerably greater value in the CIH-F3 induction group compared to the other groups. The TNF-α in lungs and PLR levels were significantly lower in the CIH-F3 group than in the CIH group. These findings suggest that FCP may serve as a functional food to mitigate the negative impact of hypoxic circumstances on lung health.
Pengaruh Ketiadaan Makan dan Minum Terhadap Kondisi Fisiologis Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Fitria, Laksmindra; Oktavia, Nurfitria; Rachid, Ayu Aziza Ar
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.12549

Abstract

Tikus (Rattus norvegicus Berkenhout, 1769) galur Wistar adalah salah satu spesies hewan coba yang lazim digunakan sebagai model dalam penelitian biomedis-praklinis. Perawatan hewan coba sangat menentukan validitas hasil penelitian. Kekurangan bahkan ketiadaan pakan dan air minum dapat terjadi saat akhir pekan maupun hari libur. Penelitian ini bertujuan untuk mempelajari pengaruh ketiadaan makan dan/atau minum terhadap kondisi fisiologis tikus Wistar. Dua belas tikus Wistar betina dewasa dibagi menjadi 4 kelompok, yaitu: Kelompok I: diberi makan dan minum ad libitum (kontrol), Kelompok II: diberi makan tetapi tidak diberi minum, Kelompok III: tidak diberi makan hanya diberi minum, dan Kelompok IV: tidak diberi makan dan minum sama sekali. Perlakuan dilakukan selama 4 hari berturut-turut, setelah itu hewan kembali diberi makan dan minum ad libitum. Data dianalisis secara statistik berdasarkan one-way repeated measures ANOVA (ɑ = 0,05). Hasil menunjukkan bahwa ketiadaan makan dan/atau minum menyebabkan kelaparan, dehidrasi, anemia, kerentanan terhadap infeksi, dan stres yang diindikasikan dengan penurunan berat badan, suhu badan, dan kadar glukosa darah, serta perubahan profil eritrosit, leukosit, dan trombosit. Meskipun sebagian analisis statistik tidak menunjukkan hasil yang signifikan, namun gangguan fisiologis ini tetap menurunkan kualitas hidup dan menimbulkan masalah kesehatan bagi hewan. Penyediaan kembali makan dan minum selama 4 hari berturut-turut mengembalikan kondisi fisiologis tikus mendekati semula. Meskipun demikian, sebaiknya tikus tetap diberi makan dan minum secara ad libitum setiap hari untuk menjaga kesehatan dan kesejahteraannya sehingga tetap layak digunakan sebagai subjek dalam penelitian. Kata kunci : kesejahteraan hewan, profil darah, puasa, Rattus norvegicus, tikus Wistar
Paparan Penyelaman Subkronik Memodulasi Profil Eritrosit dan Lipid Darah pada Tikus Wistar Putro, Helen Eko; Torop, Epa Yohana Toga; Fitria, Laksmindra; Mulyati; Rahadian Yudo Hartantyo
Berkala Ilmiah Biologi Vol 17 No 1 (2026)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Voluntary diving exercise refers to the behavior of voluntarily swimming underwater for a specific distance and duration. Diving places the body in a hypoxia state, which modulates the regulation of hematopoiesis and lipid metabolism. The effects of repeated voluntary diving exposure over a subchronic period on erythrocyte profiles and blood lipid levels in rats remain largely unknown. This study aims to investigate the effects of subchronic voluntary diving exercise on erythrocyte profiles and blood lipid levels in rats. Twenty female Wistar rats were divided into a diving group (SL) and a control group (KN). The treatment group underwent diving for 60 days, with a frequency of five training days and two rest days per week. The control group performed swimming activities over the same distance without diving and followed the same schedule. Blood samples were collected on days 0, 30, and 60 for erythrocyte profile analysis, blood plasma was collected to analyze blood lipid profile. At the end of the treatment period, the animals were euthanized and a necropsy was performed. The spleens were collected and weighed using an analytical balance. On day 60, the red blood cell count, hemoglobin, and hematocrit in the treatment group were lower than those in the control group, accompanied by increases in MCH and MCHC. These findings indicate that there were fewer circulating red blood cells but with a higher hemoglobin content per cell. These changes, along with the increase in spleen weight, indicate a physiological response to diving exposure involving the spleen and are likely related to erythrocyte redistribution (splenic sequestration). Additionally, the decrease in LDL accompanied by an increase in HDL indicates a more protective lipid profile in rats as a metabolic adaptation. Subchronic voluntary diving exercise in rats triggers physiological adaptations in the form of changes in the erythrocyte profile, increased spleen weight, and an improved lipid profile in response to repeated hypoxia exposure.
Co-Authors Abdul Razaq Chasani Aditya K. Karim Aditya Krishar Karim Andreas S. Budi, Andreas S. Anggoro Chandra Yulistiyanto Annisa Ridhowati Annisa Ridhowati, Annisa Cannadianti, Nadhira Cut M. Tiraya, Cut M. Diaz Ayu Anjani Dini Anggraini, Dini Eko Agus Suyono Eko Agus Suyono Epa Yohana Toga Torop Erlitaningsih, Ucik Gerald, Jeremia Gunawan, Isma Cahya Putri Hartantyo, Rahadian Yudo Helen Eko Putro Indah Permata Sari Indah Riwantrisna Dewi Indira Diah Utami Intan Woro Prawesti Intan Woro Prawesti, Intan Woro Isma Cahya Putri Gunawan Kholidatus Silmi Kindi Adam Kindi Adam Kristiawati, Dian Lia Lavi Illiy Lukitowati, Fajar Mahroos, Rifda El Maura Indria Meidianing Mayumi Hersasanti Meidianing, Maura Indria Meliala, Andreanyta Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Mulyati Sarto Mulyati Sarto Mulyati Sarto Mulyati Sarto Narwidina, Paramita Oktavia, Nurfitria Onny Nurrahman Marwayana Paul J. Kawatu Pricellita Nur’aivy Alvianti Putri Gunawan, Ismi Cahya Putri Nur Nur Aida Putro, Helen Eko Rachid, Ayu Aziza Ar Rahadian Yudo Rahadian Yudo Hartantyo Rahadian Yudo Hartantyo Rahadian Yudo Hartantyo Rahadian Yudo Hartantyo Rosita Dwi Putri Suranto Rury Eprilurahman Sanjaya, Wilda Bunga Tina Septy Azizah Puspitasari Siswanto Slamet Widiyanto Slamet Widiyanto Sudesty, Shellya Puti Syifa’ Aulia Rahmah Tika Candra Dewi Torop, Epa Yohana Toga Wilda Bunga Tina Sanjaya Windah Dwi Nuraini Mappalotteng