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All Journal Jurnal Akuakultur Indonesia Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Akuatika Jurnal Perikanan Kelautan Jurnal Sosial Ekonomi Pesisir Jurnal Harpodon Borneo Jurnal Matematika & Sains Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Biodjati Omni-Akuatika ALCHEMY Jurnal Penelitian Kimia Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Grouper: Jurnal Ilmiah Perikanan Jurnal Biosense International Journal of Research in Community Services Prosiding Seminar Nasional Mini Riset Mahasiswa Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Farmers: Journal of Community Services Journal Serambi Engineering (JSE) Jurnal Perikanan
Yuniar Mulyani
Fakultas Perikanan Dan Ilmu Kelautan Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PERAN KLONING GEN DALAM PENGEMBANGAN VAKSIN REKOMBINAN UNTUK PENCEGAHAN PENYAKIT PADA IKAN Aisyah; Mulyani, Yuniar
JURNAL BIOSENSE Vol 7 No 02 (2024): Edisi Desember 2024
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v7i02.4717

Abstract

Industri akuakultur menghadapi tantangan besar akibat penyakit infeksius yang dapat menurunkan produktivitas dan menyebabkan kerugian ekonomi signifikan. Salah satu pendekatan yang menjanjikan dalam meningkatkan ketahanan ikan terhadap penyakit adalah penggunaan vaksin rekombinan, yang dikembangkan melalui teknik kloning gen. Kloning gen memungkinkan identifikasi dan ekspresi antigen spesifik dari patogen ikan untuk digunakan dalam pembuatan vaksin yang lebih efektif dan aman. Vaksin rekombinan menawarkan keunggulan dibandingkan vaksin konvensional, termasuk kemudahan produksi, stabilitas yang lebih baik, dan potensi untuk mengatasi berbagai patogen secara spesifik. Dalam review ini, dibahas berbagai teknik kloning gen yang digunakan dalam pengembangan vaksin rekombinan, seperti PCR, kloning plasmid, dan CRISPR. Selain itu, dikaji pula contoh aplikasi vaksin rekombinan untuk pencegahan penyakit yang disebabkan oleh bakteri dan virus pada ikan, serta tantangan dan masa depan pengembangannya. Penelitian ini menunjukkan bahwa vaksin rekombinan memiliki potensi besar untuk meningkatkan kesehatan ikan dalam akuakultur dan berkontribusi pada keberlanjutan industri perikanan global.
Promoting the Consumption of Fish-Based Processed Products as A Strategy to Improve Children's Nutrition in Majakerta Village, Majalaya District, Bandung Regency Aisyah, Aisyah; Maulina, Ine; Mulyani, Yuniar
International Journal of Research in Community Services Vol 6, No 1 (2025)
Publisher : Research Collaboration Community (RCC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijrcs.v6i1.843

Abstract

Efforts to increase fish consumption offer numerous benefits, not only enhancing intelligence but also boosting the fisheries industry. Currently, stunting remains a concerning issue, and data indicates that fish consumption is not evenly distributed across Indonesia. This represents one of the health problems that serves as an indicator of the health and well-being of a community. One of the themes of this community service program (or also known as PPM in Indonesia) is raising awareness about improving children's nutrition among the residents of Majakerta Village, Majalaya District, Bandung Regency. The implementation of the program is conducted in collaboration with student community service (or also known as KKN in Indonesia) activities. The primary objective of this program is to improve children's nutrition through the promotion of fish-based processed food consumption and to educate mothers in Majalaya District about recognizing stunting symptoms and addressing them. The main target of this program is children aged 612 years. The method used in this awareness campaign includes educational games about fish and fish-based processed food to make the material easier for elementary school children to understand. Additionally, one type of fish-based processed food is distributed for children to taste directly, aiming to increase their interest in fish consumption. The results achieved include improved knowledge about the importance of eating fish due to its many health benefits.
The effectiveness of honey supplementation in feed for improving goldfish fingerling Carassius auratus immune system against Aeromonas hydrophila bacteria attack Rosidah, Rosidah; Subhan, Ujang; Mulyani, Yuniar; Dermawan, Rifai
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3658.789 KB) | DOI: 10.19027/jai.18.1.89-100

Abstract

ABSTRACT The attack of Aeromonas hydrophila bacteria can cause mortality in goldfish approximately 100%. The controlling of this bacterial attack can be done through increased fish immunity. Honey is one of the natural ingredients that increases body immune system. This study aimed to determine the effective dose of honey supplemented in feed to increase goldfish fingerling resistance for disease prevention. Fish used in this study were goldfish fingerlings with 3.5 g average weight. This study was done using experimental complete randomized design method with five treatments and three replications. Treatments given were honey supplementation in feed with 0 ml/kg (A) as control treatment, 150 ml/kg (B), 200 ml/kg (C), 250 ml/kg (D), and 300 ml/kg (E). The result showed that honey supplementation in feed was effective to improve goldfish fingerlings resistance against Aeromonas hydrophila bacterial attack. The supplementation of honey in feed with 200 ml/kg was the best treatment for inducing goldfish fingerlings against A. hydrophila. This was proven by the increased white blood cells (leucocytes) (27.84 ± 5.07%) followed with no apparent clinical symptoms after attacked by A.hydrophila, such as hemorrhage, necrosis, exophthalmia or dropsy, besides showing the highest survival rate with 73.33 ± 11.5%. Keywords : Aeromonas hydophila, goldfish, honey, leucocyte, resistance ABSTRAK Serangan bakteri Aeromonas hydrophila dapat menyebabkan kematian ikan mas koki hingga mencapai 100%. Penanggulangan serangan bakteri tersebut dapat dilakukan dengan meningkatkan ketahanan tubuh (imun) ikan.  Madu merupakan salah satu bahan alami yang dapat meningkatkan ketahanan tubuh. Penelitian ini bertujuan untuk menentukan dosis efektif penambahan madu pada pakan untuk meningkatkan daya tahan tubuh benih ikan mas koki dalam upaya pencegahan penyakit aeromonasis. Benih yang digunakan adalah benih ikan mas koki berukuran 3.5 gram. Metode penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan madu pada pakan dengan dosis 0 mL/kg (A) sebagai kontrol, 150 mL/kg (B), 200 mL/kg (C), 250 mL/kg (D), 300 mL/kg (E). Hasil penelitian menunjukkan bahwa penambahan madu ke dalam pakan efektif dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan Aeromonas hydrophila. Dosis 200 ml/kg pakan memberikan hasil terbaik dalam meningkatkan ketahanan tubuh ikan mas koki terhadap serangan A. hydrophila terlihat dari peningkatan jumlah sel darah putih terbesar (27.84 ± 5.07%), tidak nampak adanya gejala klinis ikan terserang A. hydrophila seperti hemoragi, necrosis, exophthalmia maupun dropsy dan menghasilkan kelangsungan hidup benih ikan mas koki tertinggi yaitu sebesar 73.33 ± 11.5%. Kata kunci: Aeromonas hydrophila, ketahanan tubuh, ikan mas koki, madu, sel darah putih. 
EFFECT OF MICROENCAPSULATED Lactobacillus sp. ON CATFISH (Pangasius hypophthalmus) INFECTED WITH Aeromonas sp. Kharima, Zhafira Putri; Mulyani, Yuniar; Iskandar, Iskandar; Herman, Roffi Grandiosa
Aqusains: Jurnal Ilmu Perikanan dan Sumberdaya Perairan Vol. 13 No. 1 (2024): November 2024
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aqs.v13i1.p1575-1588

Abstract

The prevention of fish infections caused by Aeromonas sp. bacteria can be achieved using Lactobacillus sp. bacteria. This study aims to identify and analyse the effect of Lactobacillus sp. using the microencapsulation method to treat catfish infected with Aeromonas sp. The identification of Lactobacillus sp. bacteria was conducted through microbiological and molecular tests. After obtaining the test results, the research continued with the application of microencapsulation using the emulsion technique. The impact of this microencapsulation was evaluated on catfish through an experimental approach employing a completely randomised design (CRD), with 10 catfish tested in each experimental aquarium. The study included five treatments, designated as A, B, C, D, and E. Treatment A served as the control, without the administration of Lactobacillus. Treatment B involved the application of Lactobacillus without microencapsulation. Treatments C, D, and E utilised varying doses of Lactobacillus at 0.003, 0.005, and 0.007 g/L, respectively. Each treatment was conducted in triplicate. Microbiological and molecular testing identified encapsulated Lactobacillus sp., specifically Lactobacillus helveticus and Lactobacillus johnsonii. The results indicated that treatment D, comprising a dose of 0.005 g/L of Lactobacillus helveticus and Lactobacillus johnsonii, was the most effective in promoting the healing of catfish infected with Aeromonas hydrophila bacteria.Keywords: Completely randomized design, bacterial infection mitigation, probiotic bacteria, emulsion technique.
EFFECTS OF Bacillus sp PROBIOTICS ON GROWTH AND SURVIVAL OF Carassius auratus IN URBAN FISH FARMING Syakir, Muhammad Sabiq; Mulyani, Yuniar; Rostika, Rita; Haetami, Kiki; Davinci, Leonardo; Karsih, Okta Rizal
Asian Journal of Aquatic Sciences Vol. 8 No. 2 (2025): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.2.214-222

Abstract

This study aims to determine the optimum density of Bacillus sp. bacteria applied to feed to increase the growth of C. auratus maintained in a fish farming system in an 80 L bucket. As a form of urban farming, this research was conducted in the yard of Building 1 of the Faculty of Fisheries and Marine Sciences, UNPAD. This study used an experimental method with a Completely Randomized Design model consisting of 4 treatments and three replications, namely treatment A (control), treatment B (Bacillus sp 106 CFU/mL), treatment C (Bacillus sp. 108 CFU/mL), and treatment D (Bacillus sp 1010 CFU/mL). Observations were made for 60 days. Observation parameters were weight growth, length growth, specific growth rate, feed efficiency, survival, and water quality. The results of this study indicate that treatment C (Bacillus sp 108 CFU/mL) was the most effective in increasing the growth of C. auratus, as seen from the absolute weight growth of 61.20 g, absolute length growth of 1.30 cm, specific growth rate of 0.77%/day, feed efficiency of 38%, and fish survival rate of 96% which were significantly different compared to the others.
Pemanfaatan Sistem IOT Dan Panel Surya Dalam Akuaponik Untuk Memanfaatkan Lahan Terbatas Serta Mengukur Produktivitas Rahidin, Umar; Maulina, Ine; Mulyani, Yuniar
Prosiding Seminar Nasional Mini Riset Mahasiswa Vol 3, No 2 (2024)
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Internet of thing (IoT) merupakan suatu konsep yang bertujuan untuk memperluas manfaat dari konektivitas internet yang tersambung secara terus menerus. Sedangkan panel surya adalah kumpulan sel surya yang disusun untuk menyerap sinar matahari secara efektif. Sel surya bertugas menyerap sinar matahari. Sel surya sendiri terdiri dari berbagai komponen photovoltaic, atau komponen yang dapat mengubah cahaya menjadi listrik. Panel surya digunakan untuk membantu dalam menghemat penggunaan listrik selama budidaya. Gabungan Internet of thing (IoT) dan panel surya dimanfaatkan pada budidaya ikan agar mempermudah pembudidaya serta menghemat pengeluaran selama proses budidaya. Penelitian ini bertujuan untuk mempermudah budidaya ikan dan tanaman dalam tempat yang sama di lahan sempit serta untuk mengetahui dampak dari penggunaan IoT dan panel surya terhadap produkvitas. Penelitian dilakukan dengan metode deskriptif eksploratif dan analitis dimana dalam penelitian ini sitem akuaponik diharapkan dapat meminimalisir waste pada budidaya perikanan. Adapun konsep akuaponik yang dirancang pada penelitian ini adalah NFT (Nutrient Film Technique ) dimana pada konsep ini pipa dipasang secara horizontal agar air dapat mengalir dengan baik. Hasil yang didapat, penggunaan IoT (Internet of thing) menunjukan hasil yang baik, dimana ikan lele sangkuriang memiliki bobot total 1044 g dengan 50,6 g kangkong sedangkan pada akuaponik konvensional sebesar 955 g ikan lele dan 22,9 g kangkung.
Promoting the Consumption of Fish-Based Processed Products as A Strategy to Improve Children's Nutrition in Majakerta Village, Majalaya District, Bandung Regency Aisyah, Aisyah; Maulina, Ine; Mulyani, Yuniar
International Journal of Research in Community Services Vol. 6 No. 1 (2025)
Publisher : Research Collaboration Community (Rescollacom)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijrcs.v6i1.843

Abstract

Efforts to increase fish consumption offer numerous benefits, not only enhancing intelligence but also boosting the fisheries industry. Currently, stunting remains a concerning issue, and data indicates that fish consumption is not evenly distributed across Indonesia. This represents one of the health problems that serves as an indicator of the health and well-being of a community. One of the themes of this community service program (or also known as PPM in Indonesia) is raising awareness about improving children's nutrition among the residents of Majakerta Village, Majalaya District, Bandung Regency. The implementation of the program is conducted in collaboration with student community service (or also known as KKN in Indonesia) activities. The primary objective of this program is to improve children's nutrition through the promotion of fish-based processed food consumption and to educate mothers in Majalaya District about recognizing stunting symptoms and addressing them. The main target of this program is children aged 612 years. The method used in this awareness campaign includes educational games about fish and fish-based processed food to make the material easier for elementary school children to understand. Additionally, one type of fish-based processed food is distributed for children to taste directly, aiming to increase their interest in fish consumption. The results achieved include improved knowledge about the importance of eating fish due to its many health benefits.
Intensity and Prevalence of Ectoparasites in Carp in Cirata Reservoir KJA, Cianjur Regency, West Java Sri Lusi Mulyani; Mulyani, Yuniar; Rochima, Emma; Roffi Grandiosa
Grouper Vol. 16 No. 1 (2025): Grouper : Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v16i1.275

Abstract

One of the problems that arise in fish farming is the occurrence of disease attacks that can cause losses to farmers. Parasites that are classified as ectoparasites themselves usually live outside the host. The purpose of this study was to analyze the level of intensity and prevalence of ectoparasites in goldfish in Floating Net Cages (KJA). The study used a survey method, fish samples were taken using purposive sampling method. The results showed that the highest intensity value at station 1 was Tricodina sp. 7 ind/head, station 2 was Ichthyophtirius multifilis which was 2.5 ind/head, station 3 was Dactylogyrus sp. which was 2.75 ind/head with a low-medium category. The lowest intensity value is station 1 Lernea sp. and Ergasilus sp. 1 ind/head, station 2 namely Vorticella sp. and Gyrodactylus sp. namely 1 ind/head, station 3 Chilodonella sp. and Gyrodactylogyrus sp. namely 1 ind/head with a low category. The prevalence value of station 1 is Dactylogyrus sp. 20%, station 2 Dactylogyrus sp. 20.00%, station 3 Dactylogyrus sp. 20.00% with frequent category. The lowest station 1 Lernea sp. 3.33%, Tricodina sp. 3.33%, Ergasilus sp. 3.33%, and Ichthyophtirius multifilis 3.33%. Station 2 Chilodonella sp. 3.33%, and Vorticella sp. 3.33%. Station 3 Gyrodactylus sp. 3.33% with the category sometimes.
Intensity and Prevalence Trichodina sp. on Tilapia Fish Seeds (Oreochromis niloticus) at the Jatiwangi Cultivation Pond, Majalengka Erna Nurhasanah; Mulyani, Yuniar; Zidni, Irfan; Grandiosa, Roffi
Grouper Vol. 16 No. 1 (2025): Grouper : Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v16i1.276

Abstract

The aim of this research is to determine the intensity and prevalence values ​​of Trichodina sp., using the scrapping method. Time and location of research from May to June 2024 at the Aquaculture Laboratory of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran and the Parasitology Laboratory at the Cirebon Marine and Fishery Products Quality Control and Supervision Agency (BPPMHKP). Exploratory-descriptive research method. Morphological identification using random sampling is identified microscopically. The results of the research obtained an intensity value of 31.8 individuals/head. Meanwhile, the prevalence value was 79.16%. There are 5 species of Trichodina sp. found were Trichodina acuta, Trichodina heterodentata, Trichodina nobilis, Trichodina reticulata, and Trichodina nigra.
APLIKASI BAKTERI PROBIOTIK PADA PAKAN IKAN ., Aisyah; Haetami, Kiki; Andriani, Yuli; Mulyani, Yuniar
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 10 No 1 (2022): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.76 KB) | DOI: 10.29406/jr.v10i1.3353

Abstract

Pakan merupakan aspek yang sangat penting dalam budidaya. Pentingnya efisiensi dalam produksi pakan sangat diperlukan untuk menekan biaya produksi. Salah satunya adalah penambahan probiotik Probiotik merupakan feed additive yang memiliki efek positif bagi kesehatan ikan karena dapat meningkatkan keseimbangan mikrobiota usus serta memberikan perlindungan dan perlindungan terhadap penyakit.Mengandung banyak mikroorganisme. Meningkatkan kecernaan pakan mendorong pertumbuhan dan meningkatkan kekebalan tubuh terhadap patogen tertentu. Sumber probiotik diperoleh melalui proses isolasi spesies bakteri dan karakterisasi kemudian diuji pada ikan yang diuji. Selain itu, saat memberikan probiotik pada ikan, ada dua jenis, cair dan bubuk, keduanya memiliki efek yang sama. Oleh karena itu, penggunaan probiotik ini harus diterapkan secara luas untuk meningkatkan kualitas pakan.
Co-Authors Afifah Shabirah Agus Purwanto Aisyah Aisyah . Aisyah . Aisyah Aisyah Aisyah Aisyah Aisyah Aisyah Aldhi Rahmadianto Alin Shelina Nurashila Anqy Riyanto Arvel Savero Kusuma Chitra Devi Amelia Dachlan, Dara Tasya Zallika Davinci, Leonardo Dermawan, Rifai Dian Yuni Pratiwi Eddy Afrianto Elvin Giantara Muharam Emma Rochima Erna Nurhasanah Eulis Reni Sundari Evi Liviawaty Fiddy Semba Prasetiya Fittrie Mellianawaty Pratiwy Ghina Ilmia Hafshah Handaka, Asep Agus Henhen Suherman I Nyoman Pugeg Aryantha Ibnu Dwi Buwono Iis Rostini Imtiyaz Nur Imtiyaz Nur Shadrina Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Ine Maulina Iskandar Iskandar Iskandar Iskandar Iskarimah Yolanisa Isni Nurruhwati Iwang Gumilar Juli A Sinaga Karimah Syakirotin Karsih, Okta Rizal Kharima, Zhafira Putri Khasanatur Rosyidah Kiki Haetami Luthfi Nur Aulia Miftahul Agra Putri Mochamad Untung K. Agung Mochamad Untung Kurnia Agung Muhammad Luthfi Ramadhan Muyassar, Nabil Fathi Nono Carsono Nur , Imtiyaz Nur Aulia, Luthfi Nurfitri Nurfitri Petrus Putra Bagaskhara Putri Indah Ayuningrum Putri, Miftahul Agra Rahidin, Umar Rahmat Ariffudin Rita Rostika Rizka Nurfadillah Roffi Grandiosa Roffi Grandiosa Roffi Grandiosa Herman Rosidah - Rosidah Rosidah Rosidah Rosidah Rudy Kurniadi Rufi'i Rusky Intan Pratama Shadrina, Imtiyaz Nur Sri Lusi Mulyani Sulastri Arsad Sundari, Eulis Reni Syafrudin Lewaru Syakir, Muhammad Sabiq Thania Tiaraningsih Titin Herawati Ujang Subhan Walim Lili Yeni Mulyani Yuli Andriani Zahidah Zahidah Zidni, Irfan Zuzy Anna