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ANALISIS KEKERABATAN GENETIK PADA IKAN DEWA (MAHSEER) DARI SUNGAI DI PULAU JAWA MENGGUNAKAN GEN COI Muyassar, Nabil Fathi; Mulyani, Yuniar; Zidni, Irfan; Herawati, Titin
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 21, No 4 (2025): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.21.4.238-247

Abstract

Ikan dewa (mahseer) penting untuk konservasi di Pulau Jawa karena tingginya nilai ekonomis dan ekologis, sehingga diperlukan analisis kekerabatan genetik. Penelitian dilakukan di Sungai Sungapan, Sungai Cimanuk, Curug Tonjong, Curug Cimedang, Sungai Ciwulan, Sungai Serayu, Sungai Progo, Sungai Samin, Sungai Kedung Minten, dan Sungai Brantas di pulau Jawa, bertujuan menganalisis kekerabatan genetik ikan dewa. Metode yang digunakan adalah deskriptif kualitatif dengan pengambilan sampel acak dari sungai di pulau Jawa. Identifikasi molekuler menggunakan gen COI (Cytochrome Oxidase I) untuk menganalisis jarak dan kekerabatan genetik, lalu data dianalisis secara deskriptif. Hasil elektroforesis menunjukkan amplikon ±700 bp dari gen COI untuk semua sampel. Analisis sekuens menggunakan BOLD System mengidentifikasi sampel memiliki kemiripan dengan Tor tambra dan Neolissochilus soro. Analisis genetik menggunakan MEGA11 menunjukkan jarak genetik antara spesies Tor sp. dengan spesies Neolissochilus sp. sebesar 0,0-3,2%, mengindikasikan perbedaan spesies. Tor sp. dari beberapa sungai menunjukkan kekerabatan dekat (≤1%) dengan spesies Tor lainnya (T. tambra, T. mekongensis, T. dongnaiensis). Namun, Neolissochilus sp. dari sungai lain memiliki jarak genetik tinggi (hingga 6%) dengan genus Neolissochilus dari GeneBank. Analisis filogenetik memperkuat hasil ini, mengelompokkan sampel menjadi dua klaster monofiletik yang berbeda secara genetik, yaitu klaster Tor sp. yang berkerabat dekat dengan T. tambra, dan klaster Neolissochilus sp. yang terpisah dan berkerabat jauh dengan spesies Neolissochilus lain yang tersedia di GeneBank.
Antibacterial Activity of Avicennia Mangrove Yeni Mulyani; Yuniar Mulyani; Aisyah Aisyah
ALCHEMY Jurnal Penelitian Kimia Vol 21, No 2 (2025): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.21.2.100706.180-197

Abstract

The rise of antibiotic resistance presents a major challenge, reducing the efficacy of conventional antibacterial treatments and necessitating the discovery of novel antimicrobial agents. The use of natural products has played a pivotal role in the development of antibiotics. Specifically, marine organisms, with a notable emphasis on mangroves of the genus Avicennia, have played a crucial role in this process. Avicennia marina, Avicennia officinalis, Avicennia alba, and Avicennia germinans have been found to contain secondary metabolites, including flavonoids, tannins, alkaloids, and terpenoids, which exhibit antibacterial properties against drug-resistant pathogens. The review was conducted based on literature published between 2005 and 2025. These compounds act through diverse mechanisms such as disrupting bacterial cell walls, inhibiting protein synthesis, and interfering with quorum sensing and biofilm formation. Evaluations through disk diffusion, microdilution assay, and biofilm inhibition assays have demonstrated the significant antibacterial activity of Avicennia extracts, suggesting their potential as alternative therapeutics in combating resistant bacteria. Future research should focus on enhancing these bioactive compounds’ bioavailability, stability, and large-scale production while addressing potential toxicity and navigating the complex regulatory requirements for drug approval. The continued exploration of Avicennia-derived compounds may contribute to developing novel antibiotics, offering sustainable solutions to antibiotic resistance.
Genetic Relationship of the Transgenic and Non-Transgenic Mutiara Catfish with their Hybrids Sangkuriang Catfish Iskarimah Yolanisa; Ibnu Dwi Buwono; Yuniar Mulyani; Iskandar Iskandar
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4741

Abstract

The male broodstock of Transgenic Mutiara catfish (P) (containing Clarias gariepinus Growth Hormone, CgGH) is a fish who show fast growth. Propagation of the Transgenic Mutiara catfish F1 and F2 progeny involves the crossing of broodstock P with Non Transgenic Mutiara catfish. New genetic variations (polymorphisms) can be generated from crosses broodstock P and F1 which lead to the fast growth inheritance of transgenic fish. Phylogenetic relationship analysis showed distance and genetic variation in broodstock P, F1, F2 and Sangkuriang catfish (as controls) using the RAPD-PCR method (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) as the research purposes. The results showed that OPA-03 (5'-AGTCAGCCAC-3 ') primer was selective for determining phylogenetic among catfish samples. The closest genetic similarity index originates from the female broodstock of Non Transgenic Mutiara catfish (P) (93%), while the farthest originates from the male broodstock of Mutiara Transgenic catfish (P) (51%) with F1 dan F2. Closely related broodstock catfish is not recommended to be crossed.
Molecular Identification of Bacillus Derived from the Common Carp Intestine and Their Antibiotics Resistance Yuniar Mulyani; Karimah Syakirotin; Kiki Haetami; Yeni Mulyani; Evi Liviawaty; Mochamad Untung Kurnia Agung
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5263

Abstract

The use of bacteria as a biological control agent in aquaculture is such new hope overcoming the negative impacts of the impropriate use of antibiotics. Bacillus is widely known as a potential probiotic for aquaculture, especially which are derived from aquatic biota. Due to a concern that the role of Bacillus as a probiotic agent will be disrupted by residues of antibiotics in the water, this research aimed to identify Bacillus species from common carp intestine based on the molecular method and investigate their resistance to some com-mercial antibiotics that widely used in aquaculture. Chloramphenicol, ampicillin, and metronidazole were used to determine the Bacillus resistance to antibiotics using the Kirby Bauer method. The resistance assay was carried out with a bacterial density of 108 CFU/mL. The results showed that Bacillus isolates derived from common carp  intestine were closely related and resistant to antibiotics. Isolates that resistant to the three types of antibiotics were Cc.1.9 (CgN9) and Cc.2.18 (CgM18).
Analisis Implementasi Kebijakan Penangkapan Ikan Terukur di Pelabuhan Perikanan Samudera Nizam Zachman Jakarta Asep Agus Handaka Suryana; Arvel Savero Kusuma; Yuniar Mulyani; Ine Maulina
Jurnal Serambi Engineering Vol. 11 No. 3 (2026): Juli 2026
Publisher : Faculty of Engineering, Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kebijakan Penangkapan Ikan Terukur (PIT) mereformasi tata kelola perikanan tangkap Indonesia dari kontrol input menjadi kontrol output melalui tiga pilar: zonasi Wilayah Pengelolaan Perikanan (WPP), kuota hasil tangkapan, dan Penerimaan Negara Bukan Pajak (PNBP) pascaproduksi. PPS Nizam Zachman Jakarta ditetapkan sebagai lokasi prioritas implementasi PIT, namun kondisinya di lapangan belum terdokumentasi empiris. Riset ini menganalisis implementasi kebijakan berdasarkan kerangka Edwards III, kesiapan kelembagaan pelabuhan, serta respons dan kepatuhan pelaku usaha berdasarkan model Grindle, melalui desain mixed methods concurrent embedded yang menggabungkan koding kualitatif (NVivo 15) atas 13 wawancara informan aparatur dan analisis deskriptif kuesioner Likert dari 68 responden pelaku usaha (Cronbach's Alpha = 0,9022). Implementasi PIT masih parsial, hanya PNBP pascaproduksi beroperasi penuh, zonasi terbatas, dan kuota belum diterapkan. Berdasarkan pemetaan tahapan regulatif transisi kebijakan, status implementasi berada pada Tahap 4 (Transisi Lanjutan) menuju target Tahap 5 (implementasi penuh berbasis kuota). Profil Edwards III bersifat asimetris, dengan Sumber Daya sebagai variabel terkuat (113) dan Disposisi sebagai titik terlemah (34). Indikator kesiapan kelembagaan berada di bawah standar ideal regulasi, dan respons pelaku usaha menghasilkan grand mean 2,38 (47,6%; kategori Netral). Mayoritas responden tidak pernah mengikuti sosialisasi resmi dan kerap mengalami kegagalan VMS di laut.
EXPLORATION OF THE BIOACTIVE POTENTIAL OF RHIZOPHORACEAE MANGROVE PLANTS: IMPLICATIONS IN THE FIELD OF FISHERIES Yuniar Mulyani; Yeni Mulyani; Aisyah Aisyah
Vol 15 No 2 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i2.1395

Abstract

Mangrove forests, as coastal ecosystems rich in biodiversity, play an important role in ecology and the fisheries sector. Mangroves not only function as coastal protectors and habitats for aquatic organisms but also produce bioactive compounds that have potential as antibacterial, antioxidant, antifungal, and antiviral agents. Research on mangrove plants from the Rhizophoraceae family, such as Bruguiera, Kandelia, Ceriops, and Rhizophora, has shown that compounds like flavonoids, tannins, alkaloids, and saponins have significant biological activities. Flavonoids act as antimicrobials, while tannins inhibit bacterial growth by precipitating proteins. Alkaloids disrupt bacterial cell walls and have potential in combating cancer cells. Studies also reveal significant antioxidant activity, with some mangrove extracts showing great potential in combating free radicals. Moreover, these compounds offer potential in reducing reliance on synthetic antibiotics for the treatment of cultured fish diseases and provide opportunities for the development of natural-based health products. This review aims to integrate recent findings on the bioactivity of mangrove plants and propose innovative applications of bioactive compounds in fisheries and conservation with a focus on ecosystem sustainability and optimal use of natural resources.
Sanitasi dan Higienitas Tempat Pelelangan Ikan Serta Analisis Mutu Organoleptik Ikan Tongkol Lisong (Auxis rochei Risso, 1810) di Muara Angke, Jakarta Utara Thania Tiaraningsih; Rusky Intan Pratama; Yuniar Mulyani; Iis Rostini
Vol 15 No 4 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i4.1697

Abstract

The Fish Auction Place (FAP) is a facility that supports fishermen in distributing their catches. Muara Angke FAP is one of the active auction places in Jakarta. Fish is a perishable commodity that requires proper handling to maintain its quality, including hygiene and sanitation facilities, especially to preserve the quality of little tuna (Auxis rochei). This study aims to analyze the sanitation and hygiene conditions at Muara Angke FAP based on Minister of Marine Affairs and Fisheries Decree No. 1 of 2007 (KEP. KP 01/MEN/2007), assess the organoleptic quality of little tuna, and analyze the relationship between sanitation-hygiene and tuna quality. The research was conducted from January to February 2025 using a case study method. Sanitation and hygiene data were obtained using purposive sampling, while little tuna samples for organoleptic and pH tests were taken using simple random sampling. Spearman correlation was used to analyze the relationship between FAP sanitation-hygiene and tuna quality. The results show that most sanitation and hygiene aspects meet the standards, although some deficiencies remain in facilities such as handwashing stations, toilets, environmental cleanliness, and warning signs. The organoleptic test yielded a final score of 7, indicating that frozen little tuna at Muara Angke FAP is still considered fresh. The pH ranged from 5.3 to 5.7, also indicating freshness. Spearman correlation analysis showed a weak positive relationship (r= 0.281) with no significant correlation (p= 0.231) between sanitation-hygiene and the quality of little tuna.
Co-Authors Afifah Shabirah Agus Purwanto Aisyah Aisyah . Aisyah . Aisyah Aisyah Aisyah Aisyah Aisyah Aisyah Aldhi Rahmadianto Alin Shelina Nurashila Anqy Riyanto Arvel Savero Kusuma Chitra Devi Amelia Dachlan, Dara Tasya Zallika Davinci, Leonardo Dermawan, Rifai Dian Yuni Pratiwi Eddy Afrianto Elvin Giantara Muharam Emma Rochima Erna Nurhasanah Eulis Reni Sundari Evi Liviawaty Fiddy Semba Prasetiya Fittrie Mellianawaty Pratiwy Ghina Ilmia Hafshah Handaka, Asep Agus Henhen Suherman I Nyoman Pugeg Aryantha Ibnu Dwi Buwono Iis Rostini Imtiyaz Nur Imtiyaz Nur Shadrina Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Ine Maulina Iskandar Iskandar Iskandar Iskandar Iskarimah Yolanisa Isni Nurruhwati Iwang Gumilar Juli A Sinaga Karimah Syakirotin Karsih, Okta Rizal Kharima, Zhafira Putri Khasanatur Rosyidah Kiki Haetami Luthfi Nur Aulia Miftahul Agra Putri Mochamad Untung K. Agung Mochamad Untung Kurnia Agung Muhammad Luthfi Ramadhan Muyassar, Nabil Fathi Nono Carsono Nur , Imtiyaz Nur Aulia, Luthfi Nurfitri Nurfitri Petrus Putra Bagaskhara Putri Indah Ayuningrum Putri, Miftahul Agra Rahidin, Umar Rahmat Ariffudin Rita Rostika Rizka Nurfadillah Roffi Grandiosa Roffi Grandiosa Roffi Grandiosa Herman Rosidah - Rosidah Rosidah Rosidah Rosidah Rudy Kurniadi Rufi'i Rusky Intan Pratama Shadrina, Imtiyaz Nur Sri Lusi Mulyani Sulastri Arsad Sundari, Eulis Reni Syafrudin Lewaru Syakir, Muhammad Sabiq Thania Tiaraningsih Titin Herawati Ujang Subhan Walim Lili Yeni Mulyani Yuli Andriani Zahidah Zahidah Zidni, Irfan Zuzy Anna