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Intensity and Prevalence Trichodina sp. on Tilapia Fish Seeds (Oreochromis niloticus) at the Jatiwangi Cultivation Pond, Majalengka Erna Nurhasanah; Mulyani, Yuniar; Zidni, Irfan; Grandiosa, Roffi
Grouper Vol. 16 No. 1 (2025): Grouper : Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v16i1.276

Abstract

The aim of this research is to determine the intensity and prevalence values ​​of Trichodina sp., using the scrapping method. Time and location of research from May to June 2024 at the Aquaculture Laboratory of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran and the Parasitology Laboratory at the Cirebon Marine and Fishery Products Quality Control and Supervision Agency (BPPMHKP). Exploratory-descriptive research method. Morphological identification using random sampling is identified microscopically. The results of the research obtained an intensity value of 31.8 individuals/head. Meanwhile, the prevalence value was 79.16%. There are 5 species of Trichodina sp. found were Trichodina acuta, Trichodina heterodentata, Trichodina nobilis, Trichodina reticulata, and Trichodina nigra.
APLIKASI BAKTERI PROBIOTIK PADA PAKAN IKAN ., Aisyah; Haetami, Kiki; Andriani, Yuli; Mulyani, Yuniar
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 10 No 1 (2022): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.76 KB) | DOI: 10.29406/jr.v10i1.3353

Abstract

Pakan merupakan aspek yang sangat penting dalam budidaya. Pentingnya efisiensi dalam produksi pakan sangat diperlukan untuk menekan biaya produksi. Salah satunya adalah penambahan probiotik Probiotik merupakan feed additive yang memiliki efek positif bagi kesehatan ikan karena dapat meningkatkan keseimbangan mikrobiota usus serta memberikan perlindungan dan perlindungan terhadap penyakit.Mengandung banyak mikroorganisme. Meningkatkan kecernaan pakan mendorong pertumbuhan dan meningkatkan kekebalan tubuh terhadap patogen tertentu. Sumber probiotik diperoleh melalui proses isolasi spesies bakteri dan karakterisasi kemudian diuji pada ikan yang diuji. Selain itu, saat memberikan probiotik pada ikan, ada dua jenis, cair dan bubuk, keduanya memiliki efek yang sama. Oleh karena itu, penggunaan probiotik ini harus diterapkan secara luas untuk meningkatkan kualitas pakan.
ANALISIS KEKERABATAN GENETIK PADA IKAN DEWA (MAHSEER) DARI SUNGAI DI PULAU JAWA MENGGUNAKAN GEN COI Muyassar, Nabil Fathi; Mulyani, Yuniar; Zidni, Irfan; Herawati, Titin
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 21, No 4 (2025): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.21.4.238-247

Abstract

Ikan dewa (mahseer) penting untuk konservasi di Pulau Jawa karena tingginya nilai ekonomis dan ekologis, sehingga diperlukan analisis kekerabatan genetik. Penelitian dilakukan di Sungai Sungapan, Sungai Cimanuk, Curug Tonjong, Curug Cimedang, Sungai Ciwulan, Sungai Serayu, Sungai Progo, Sungai Samin, Sungai Kedung Minten, dan Sungai Brantas di pulau Jawa, bertujuan menganalisis kekerabatan genetik ikan dewa. Metode yang digunakan adalah deskriptif kualitatif dengan pengambilan sampel acak dari sungai di pulau Jawa. Identifikasi molekuler menggunakan gen COI (Cytochrome Oxidase I) untuk menganalisis jarak dan kekerabatan genetik, lalu data dianalisis secara deskriptif. Hasil elektroforesis menunjukkan amplikon ±700 bp dari gen COI untuk semua sampel. Analisis sekuens menggunakan BOLD System mengidentifikasi sampel memiliki kemiripan dengan Tor tambra dan Neolissochilus soro. Analisis genetik menggunakan MEGA11 menunjukkan jarak genetik antara spesies Tor sp. dengan spesies Neolissochilus sp. sebesar 0,0-3,2%, mengindikasikan perbedaan spesies. Tor sp. dari beberapa sungai menunjukkan kekerabatan dekat (≤1%) dengan spesies Tor lainnya (T. tambra, T. mekongensis, T. dongnaiensis). Namun, Neolissochilus sp. dari sungai lain memiliki jarak genetik tinggi (hingga 6%) dengan genus Neolissochilus dari GeneBank. Analisis filogenetik memperkuat hasil ini, mengelompokkan sampel menjadi dua klaster monofiletik yang berbeda secara genetik, yaitu klaster Tor sp. yang berkerabat dekat dengan T. tambra, dan klaster Neolissochilus sp. yang terpisah dan berkerabat jauh dengan spesies Neolissochilus lain yang tersedia di GeneBank.
Antibacterial Activity of Avicennia Mangrove Yeni Mulyani; Yuniar Mulyani; Aisyah Aisyah
ALCHEMY Jurnal Penelitian Kimia Vol 21, No 2 (2025): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.21.2.100706.180-197

Abstract

The rise of antibiotic resistance presents a major challenge, reducing the efficacy of conventional antibacterial treatments and necessitating the discovery of novel antimicrobial agents. The use of natural products has played a pivotal role in the development of antibiotics. Specifically, marine organisms, with a notable emphasis on mangroves of the genus Avicennia, have played a crucial role in this process. Avicennia marina, Avicennia officinalis, Avicennia alba, and Avicennia germinans have been found to contain secondary metabolites, including flavonoids, tannins, alkaloids, and terpenoids, which exhibit antibacterial properties against drug-resistant pathogens. The review was conducted based on literature published between 2005 and 2025. These compounds act through diverse mechanisms such as disrupting bacterial cell walls, inhibiting protein synthesis, and interfering with quorum sensing and biofilm formation. Evaluations through disk diffusion, microdilution assay, and biofilm inhibition assays have demonstrated the significant antibacterial activity of Avicennia extracts, suggesting their potential as alternative therapeutics in combating resistant bacteria. Future research should focus on enhancing these bioactive compounds’ bioavailability, stability, and large-scale production while addressing potential toxicity and navigating the complex regulatory requirements for drug approval. The continued exploration of Avicennia-derived compounds may contribute to developing novel antibiotics, offering sustainable solutions to antibiotic resistance.
Effectiveness of Lactic Acid Bacteria to Improve Cyprinus carpio Fingerlings Resistance Against Rosidah, Rosidah; Mulyani, Yuniar; Lili, Walim; Rosyidah, Khasanatur
Jurnal Biodjati Vol 5 No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.5260

Abstract

Common carp (Cyprinus carpio) cultivation is often hampered by a disease attack, one of them is the attack of Edwarsiella tarda. Lactic acid bacteria (LAB) can be used as an alternative to prevent diseases in fish by increasing the body's resistance. This research aimed to determine the most effective isolates of LAB that increase of the resistance of carp fingerlings to the attack of E. tarda bacteria and see which isolates can produce the highest survival. The LAB isolates used were the result of isolation from the gut of carp. This study used a Completely Randomized Design (CRD) consisting of four treatments with three replications. The fish were immersed with different LAB isolates, CcB7, CcB8, and CcB15 in the same density of 108 cells / mm3. Immersion was carried out for 30 minutes with a frequency of seven days. While during the research, two immersions were carried out before the challenge test against E. tarda bacteria. The parameters observed were the number of leukocytes, hematocrit, erythrocyte, differential leukocytes, survival rate, and clinical symptoms that appeared. The results showed that all LAB isolates used in this study could increase the body resistance of carp against the attacks of E. tarda bacteria. The LAB CcB7 isolate was the most effective for enhancing the body resistance of carp fish withthe highest increase level of  leukocyte, erythrocyte, and hematocrit were 18 ± 0.057,7 ± 0,077, and 0.26± 7.31% respectively. After being challenged with E. tarda bacteria producing mild clinical symptoms, the highest increase is in monocyte and neutrophil cells was 20 and 62% respectively, the highest reduction in lymphocytes was – 9%  and the highest survival rate was 80%. 
Molecular Identification of Bacillus Derived from the Common Carp Intestine and Their Antibiotics Resistance Mulyani, Yuniar; Syakirotin, Karimah; Haetami, Kiki; Mulyani, Yeni; Liviawaty, Evi; Agung, Mochamad Untung Kurnia
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5263

Abstract

The use of bacteria as a biological control agent in aquaculture is such new hope overcoming the negative impacts of the impropriate use of antibiotics. Bacillus is widely known as a potential probiotic for aquaculture, especially which are derived from aquatic biota. Due to a concern that the role of Bacillus as a probiotic agent will be disrupted by residues of antibiotics in the water, this research aimed to identify Bacillus species from common carp intestine based on the molecular method and investigate their resistance to some com-mercial antibiotics that widely used in aquaculture. Chloramphenicol, ampicillin, and metronidazole were used to determine the Bacillus resistance to antibiotics using the Kirby Bauer method. The resistance assay was carried out with a bacterial density of 108 CFU/mL. The results showed that Bacillus isolates derived from common carp  intestine were closely related and resistant to antibiotics. Isolates that resistant to the three types of antibiotics were Cc.1.9 (CgN9) and Cc.2.18 (CgM18).
Co-Authors Afifah Shabirah Agung, Mochamad Untung Kurnia Agus Purwanto Aisyah Aisyah . Aisyah . Aisyah Aisyah Aisyah Aisyah Aisyah Aisyah Aldhi Rahmadianto Alin Shelina Nurashila Anqy Riyanto Chitra Devi Amelia Dachlan, Dara Tasya Zallika Davinci, Leonardo Dermawan, Rifai Dian Yuni Pratiwi Eddy Afrianto Elvin Giantara Muharam Emma Rochima Erna Nurhasanah Eulis Reni Sundari Evi Liviawaty Fiddy Semba Prasetiya Fittrie Mellianawaty Pratiwy Ghina Ilmia Hafshah Grandiosa Herman, Roffi Handaka, Asep Agus Henhen Suherman Herman, Roffi Grandiosa I Nyoman Pugeg Aryantha Ibnu Dwi Buwono Iis Rostini Imtiyaz Nur Imtiyaz Nur Shadrina Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Ine Maulina Ine Maulina Iskandar Iskandar Iskandar Iskandar Iskarimah Yolanisa Isni Nurruhwati Iwang Gumilar Juli A Sinaga Karimah Syakirotin Karsih, Okta Rizal Kharima, Zhafira Putri Khasanatur Rosyidah Kiki Haetami Kiki Haetami Kurnia Agung, Mochamad Untung Luthfi Nur Aulia Mochamad Untung K. Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Mochamad Untung Kurnia Agung Muhammad Luthfi Ramadhan Muyassar, Nabil Fathi Nono Carsono Nur , Imtiyaz Nur Aulia, Luthfi Nurfitri Nurfitri Petrus Putra Bagaskhara Putri Indah Ayuningrum Putri, Miftahul Agra Rahidin, Umar Rahmat Ariffudin Rita Rostika Rizka Nurfadillah Roffi Grandiosa Roffi Grandiosa Roffi Grandiosa Herman Rosidah - Rosidah Rosidah Rosidah Rosidah Rosyidah, Khasanatur Rudy Kurniadi Rusky Intan Pratama Shadrina, Imtiyaz Nur Sri Lusi Mulyani Sulastri Arsad Sundari, Eulis Reni Syafrudin Lewaru Syakir, Muhammad Sabiq Syakirotin, Karimah Tiaraningsih, Thania Titin Herawati Ujang Subhan Walim Lili Yeni Mulyani Yolanisa, Iskarimah Yuli Andriani Yuli Andriani Zahidah - Zidni, Irfan Zuzy Anna