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All Journal International Journal of Public Health Science (IJPHS) SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Riset Kesehatan Jurnal Surya Medika Jurnal Labora Medika JURNAL ANALIS LABORATORIUM MEDIK Jaringan Laboratorium Medis ARTERI : Jurnal Ilmu Kesehatan Borneo Journal of Medical Laboratory Technology Abdimasku : Jurnal Pengabdian Masyarakat Jurnal Health Sains Jurnal Abdi Masyarakat Indonesia Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Aurelia: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia Jurnal Pengabdian Pada Masyarakat Gema Lingkungan Kesehatan Jurnal Ilmiah Pengabdian Masyarakat Bidang Kesehatan Jurnal Dunia Ilmu Kesehatan Jurnal Laboratorium Khatulistiwa Poltekita: Jurnal Ilmu Kesehatan Jurnal Sains dan Kesehatan
Eko Naning Sofyanita
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Semarang, Semarang, Central Java, Indonesia
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Journal : Borneo Journal of Medical Laboratory Technology

 1 
The Effect of Light Exposure to Bilirubine Levels on Serum Jaundice Infant in Hospital of Islamic NU Demak Eko Naning Sofyanita; Endang Susilowaty; Roni Afriansya
Borneo Journal of Medical Laboratory Technology Vol 3 No 1 (2020): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i1.1766

Abstract

Bilirubin is a substance formed from the normal breakdown of erythrocytes in the body so that it gives a yellow color to the stool and urine. The test of bilirubin in the laboratory must avoid exposure to light, which can cause decreased serum bilirubin levels by up to 50% in 1 hour due to disruption of the bilirubin's stability. This study is a cross-sectional analytic study of primary data using 30 samples of jaundice baby serum and direct and total bilirubin test. Data collection was conducted from the primary data by a direct test using 40 samples of infant jaundice and test of direct bilirubin and total bilirubin using methods Dichlorophenyl Diazonium. The results of the tests of 40 samples can result in the mean levels of total bilirubin were exposed to the light of 8.58 mg/dl and were not exposed to light 12,67mg /dl. Direct bilirubin levels mean that exposure to light is 3.98 mg/dl. In contrast, the unexposed light of 8.71 mg/dl, so that it can be concluded that the levels of total and direct bilirubin in serum jaundice infants exposed to lower light compared with those not exposed to light.
Hubungan Jumlah Eritrosit dan Hitung Jenis Leukosit pada Masyarakat dengan Paparan Polutan Tinggi di Desa Tambak Mulyo Kota Semarang Eko Naning Sofyanita; Hendra Adistia Bangkit; Roni Afriansya
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2230

Abstract

Lead (Pb) and carbon monoxide (CO) are gas components in airborne pollutants due to vehicle fumes or industrial exhaust gases that affect the blood, namely shortening the life span of erythrocytes produced in erythropoiesis. In addition, small pollutants can be inhaled and enter the alveoli and then phagocytosis and trigger the release of mediators, namely leukocyte cells, which can cause inflammation. This study aims to determine the correlation between the erythrocytes count and differential leucocyte count of the Tambak Mulyo village community, Semarang City. This research was a cross-sectional analytic study of primary data using 20 blood samples, carried out by the erythrocytes count and differential leucocyte count tests. The examination results showed that the average erythrocytes count in all samples was 5.25 million cells/mm3 with a percentage of the normal erythrocytes count is 45.00%, and a high of erythrocytes count is 55.0%. On the test of the differential leucocyte count, 4 samples are obtained with specific values. Normal leukocytes and as many as 16 samples experienced an increase and decrease in the differential leucocyte count. The chi-square test shows Asymp.Sig> 0.05, which means there is no correlation between variables, so it can be concluded that there is no significant correlation between the erythrocytes count and differential leucocyte count (lymphocytes, basophils, eosinophils, neutrophils, and monocytes) in the community with exposure to high pollutants in Tambak Mulyo village, Semarang City.
Correlation Of Erythrocyte Sedimentation Rate (ESR) and Lymphocyte Counts in Pulmonary Tuberculosis Patients during Intensive Treatment in Purwokerto City Eko Naning Sofyanita
Borneo Journal of Medical Laboratory Technology Vol 4 No 1 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i1.2722

Abstract

Pulmonary tuberculosis is a disease caused by the bacterium Mycobacterium tuberculosis which most often attacks the lungs by spreading through the air in patients who cough, sneeze or spit. In tuberculosis, the erythrocyte sedimentation rate examination is used to help diagnose the course of the disease and help for the success of chronic therapy, and the examination of the lymphocyte count is used to support the diagnosis of bacterial infection. This study is an analytic study through a cross-sectional approach with the Pearson Correlation test. The results of this LED examination were 8 samples (21%) normal and 30 samples (79%) abnormal. On examination of the lymphocyte count, 21 samples (55%) had lymphopenia, 16 samples (42%) were normal patients, and 1 sample (3%) had lymphocytosis. In the Pearson correlation test, the correlation is unidirectional but moderately or moderately correlated (r=0.304). Based on these results, it can be concluded that there is a relationship between erythrocyte sedimentation rate and lymphocyte count. This means that if the LED value is large, the number of lymphocytes will also increase.
Efektifitas Penggunaan Deterjen Laundry Cair sebagai Agen Deparafinisasi pada Sediaan Ginjal Mencit (Mus musculus): Effectiveness of Using Liquid Laundry Detergent as a Deparaffinizing Agent in Mice Kidney Preparations Faiz Shofa Abdillah; Eko Naning Sofyanita
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5100

Abstract

Deparaffinization is one of the stages of tissue processing, which aims to dissolve paraffin for full-color application. Deparaffinization can only be done by xylol to dissolve paraffin as fat. Liquid laundry detergent has the main component in the form of a surfactant which has the property of removing types of lipids by dissolving and emulsification because xylol has carcinogenic properties, flammable properties, and is relatively expensive. This study aimed to describe the quality of tissue preparations using liquid laundry detergents 1.5%, 3%, and 4.5% as deparaffinized agents and also to describe and compare several concentrations of the most effective laundry detergents as deparaffinized agents. Results from the preparation quality using xylol as a deparaffinized agent by conducting an experimental study with a cross-sectional design showed promising results. The quality of preparations using 1.5% liquid laundry detergent got poor results. The quality of preparations using 3% liquid laundry detergent got poor results. The quality of preparations using 4.5% liquid laundry detergent got good results. Based on the results of the Kurskal Wallis test with a significance value of p <0.05, which indicates that H0 is rejected, the conclusion that can be drawn is that there is an unfavorable difference in results between xylol and liquid laundry detergent as a deparaffinized agent.
Perbedaan Hasil Pewarnaan Hematoxylin Eosin (HE) Pada Histologi Kulit Mencit (Mus Musculus) Berdasarkan Ketebalan Pemotongan 3 Μm, 6 Μm Dan 9 Μm Nazhiifah, Tristania Shofi; Sofyanita, Eko Naning
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6090

Abstract

Sectioning is a step that must be passed before staining Hematoxylin Eosin (HE). The aim of this study was to determine differences in the results of Hematoxylin Eosin (HE) staining in mice skin histology (Mus musculus) based on the thickness of the microtome sections of 3 μm, 6 μm, and 9 μm. Experimental research, true experimental research design post test only control group design. The research sample was mice skin preparations (Mus musculus). Primary data collection, cutting of skin preparations using a microtome and staining Hematoxylin Eosin (HE). Field readings were carried out at 400x magnification (40x objective). Data was processed using Kruskal Wallis and Man Whitney. The average value of 6 μm nuclear cutting was 1.44, cytoplasm was 1.15 and color uniformity was 3. The results showed an abnormal distribution, the Kruskal Wallis test (p=0.008) there was a difference in the quality of staining in the 3 μm, 6 μm and 9 μm. The Man Whitney test for the 3 μm and 6 μm microtome cutting groups (p=0.412) showed no difference in the group, the 6 μm and 9 μm microtome cutting groups (p=0.004) there were differences in the group, the 3 μm and 9 μm cutting groups ( p=0.004) there was a difference in the cutting group. The conclusion of this study was that 6 μm microtome cutting produced preparations with better staining quality compared to 3 μm and 9 μm microtome cutting.
Co-Authors Achmad Zulfa Juniarto Achmad Zulfa Juniarto Aniesa Izzatun Nafsi Anna Sufi Annisa Anung Sugihartono Ari Suwondo Ari Suwondo Arya Iswara Arya Iswara Auliya, Qurrotu A'yuni Azahra, Nabila Burhannudin Dina Afrianti1 Djoko Priyatno Djoko Priyatno, Djoko Duri, Iin Demiany Elis Susilawati Endang Susilowaty Errina Septiyasari Faiz Shofa Abdillah Hadipranoto, Ichsan Hendra Adistia Bangkit Heri Nugroho Heri Nugroho I Made Wisnu Darma Putra Ichsan Hadipranoto Lilik Setyowatiningsih Maulana, Mochamad Rizal Nabila, Jihan Nanik Aryani Putri Nazhiifah, Tristania Shofi Nur Indah Palupi NURUL QOMARIYAH Nurul Qomariyah Nurul Qomariyah Palupi, Nur Indah PUJI LESTARI Putri Kurniasiwi Qurrotu A’yuni Auliya Raden Mohamad Herdian Bhakti Rihanesa Diana Putri Ririh Jatmi Wikandari Rodhi Hartono Roni Afriansya Rosidah, Umi S.Y. Didik Widiyanto Setyowatiningsih, Lilik Siti Thomas Zulaikhah Siwi, Utami Purnama Susilawati, Elis Swastya Putri, Adita Puspitasari Swastyaputri, Adita Puspitasari Syanubari, Sonnia Pusparindah Taufiqurrachman Nasihun Umi Rosidah WAHYUDI Widiyanto, S.Y. Didik