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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease journal of Basic Medical Veterinary Jurnal Kedokteran Hewan
Didik Handijatno
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Exploration of the Antibacterial Potential from Rice Eel Skin Mucus (Monopterus albus) Against Bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus Praja, Ratih Novita; Yudhana, Aditya; Handijatno, Didik; Al-Madinah, Wardatul Qoryah; Hamonangan, Jonathan Mark; Insani, Alivia Khairina; Prameswari, Nindya Pradnya; Prasetyo, Dhenatra Rifqy; Praja, Shifa Salsabilla
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.197-205

Abstract

The skin mucus of the rice eel (Monopterus albus) contains various antibacterial compounds and has the potential as a synthetic antibiotic. This research was conducted to explore the potential antibacterial power of the rice eel skin mucus against some pathogenic bacteria in freshwater fish. The bacteria were isolated from five samples of rice eel cultivation ponds belonging to Mr. Sabwan and then challenged with the mucus of the eel's skin through diffusion tests using paper discs. The rice eel skin mucus tested its antibacterial activity against three species of freshwater bacteria, Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus, by testing sensitivity and inhibitory zones. The inhibitory zones of each bacterium were measured using Vernier caliper which refers to the standardization of the inhibitory zone: < 4 mm no activity, 5-9 mm weak, 10- 14 mm medium, and > 15 mm strong. Test results showed that the eels' skin mucus could inhibit the growth of bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus.
Co-Authors . Suwarno Adikara, Tatang Santanu Agnes Theresia Soelih Estoepangestie Al-Madinah, Wardatul Qoryah Ayang Mahindra Christian Marco Hadi Nugroho Desiandura, Kurnia Elisa Herina Dimariwu Endang Suprihati Estoepangestie, Agnes Theresia Soelih Fedik Abdul Rantam Hamonangan, Jonathan Mark Hani Plumeriastuti Harijani, Nenny Insani, Alivia Khairina Jola Rahmahani Lucia Tri Suwanti, Lucia Tri Maria Gladis Bupu Maze Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nunuk Dyah Retno Lastuti Praja, Ratih Novita Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prasetyo, Dhenatra Rifqy Prestalia Dwi Rachmawati Rahaju Ernawati Raharjo, Dadik Ratih Ratnasari Romy Muhammad Dary Mufa Setiawan Koesdarto Tyasningsih, Wiwiek Wiwik Tyasningsih Yudhana, Aditya