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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease journal of Basic Medical Veterinary Jurnal Kedokteran Hewan
Didik Handijatno
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Exploration of the Antibacterial Potential from Rice Eel Skin Mucus (Monopterus albus) Against Bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus Praja, Ratih Novita; Yudhana, Aditya; Handijatno, Didik; Al-Madinah, Wardatul Qoryah; Hamonangan, Jonathan Mark; Insani, Alivia Khairina; Prameswari, Nindya Pradnya; Prasetyo, Dhenatra Rifqy; Praja, Shifa Salsabilla
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.197-205

Abstract

The skin mucus of the rice eel (Monopterus albus) contains various antibacterial compounds and has the potential as a synthetic antibiotic. This research was conducted to explore the potential antibacterial power of the rice eel skin mucus against some pathogenic bacteria in freshwater fish. The bacteria were isolated from five samples of rice eel cultivation ponds belonging to Mr. Sabwan and then challenged with the mucus of the eel's skin through diffusion tests using paper discs. The rice eel skin mucus tested its antibacterial activity against three species of freshwater bacteria, Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus, by testing sensitivity and inhibitory zones. The inhibitory zones of each bacterium were measured using Vernier caliper which refers to the standardization of the inhibitory zone: < 4 mm no activity, 5-9 mm weak, 10- 14 mm medium, and > 15 mm strong. Test results showed that the eels' skin mucus could inhibit the growth of bacteria Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus.
CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Praja, Ratih Novita; Handijatno, Didik; Koesdarto, Setiawan; Yudhana, Aditya
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Co-Authors . Suwarno Adikara, Tatang Santanu Agnes Theresia Soelih Estoepangestie Al-Madinah, Wardatul Qoryah Ayang Mahindra Christian Marco Hadi Nugroho Desiandura, Kurnia Elisa Herina Dimariwu Endang Suprihati Estoepangestie, Agnes Theresia Soelih Fedik Abdul Rantam Hamonangan, Jonathan Mark Hani Plumeriastuti Harijani, Nenny Insani, Alivia Khairina Lucia Tri Suwanti, Lucia Tri Maria Gladis Bupu Maze Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nunuk Dyah Retno Lastuti Praja, Ratih Novita Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prasetyo, Dhenatra Rifqy Prestalia Dwi Rachmawati Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Ratih Ratnasari Romy Muhammad Dary Mufa Setiawan Koesdarto Tyasningsih, Wiwiek Wiwik Tyasningsih Yudhana, Aditya