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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Vitek : Bidang Kedokteran Hewan Journal of halal product and research (JHPR) journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine
Didik Handijatno
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Praja, Ratih Novita; Handijatno, Didik; Koesdarto, Setiawan; Yudhana, Aditya
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Co-Authors . Suwarno A. T. Soelih Estoepangestie Adikara, Tatang Santanu Agnes Theresia Soelih Estoepangestie Al-Madinah, Wardatul Qoryah Ayang Mahindra Christian Marco Hadi Nugroho Desiandura, Kurnia Desty Apritya Elisa Herina Dimariwu Emmanuel Djoko Poetranto Endang Suprihati Fedik Abdul Rantam Hamonangan, Jonathan Mark Hani Plumeriastuti Illa Rohdiana Hermawati Ine Karni Insani, Alivia Khairina Kusnoto Kusnoto Lailatul Maghfiroh Lucia Tri Suwanti, Lucia Tri Maria Gladis Bupu Maze Mufasirin Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nenny Harijani Nunuk Dyah Retno Lastuti Praja, Ratih Novita Praja, Shifa Salsabilla Prameswari, Nindya Pradnya Prasetyo, Dhenatra Rifqy Prestalia Dwi Rachmawati Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Ratih Ratnasari Ratih Ratnasari Residiwati, Gretania Rima Ratnanggana Prasetya Romy Muhammad Dary Mufa Setiawan Koesdarto Sudarno, Sudarno Tri Nurhajati Tyasningsih, Wiwiek Wiwik Tyasningsih Yudhana, Aditya