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Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan Jenis Kelamin Anak Sesuai Harapan Dasrul Dasrul; M. Aman Yaman; Zulfan Zulfan
Jurnal Agripet Vol 13, No 1 (2013): Volume 13, No. 1, April 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i1.546

Abstract

Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purposeABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the spermatozoa quality, sex ratio between X and Y, capacity of fertility indicated by conception rate and sex ratio of goat boar kids. Samples, used in this experiment, are fresh semen from Boer goat with high quality consists of 4 group treatments with 6 replications 1) group of spermatozoa without separation (control), 2) group of spermatozoa separated by percoll gradient density centrifugation 3 levels (P1), 5 levels (P2) and swine up (P3). The observed parameters are spermatozoa quality, X and Y spermatozoa ratio, fertilitys capacity and sex ratio on the birth. Quality examination of spermatozoa and identify X and Y spermatozoa is based on the standard method of WHO. The conception rate was based on the ratio of pregnant goat after the first insemination. Data of spermatozoa quality and spermatozoa ratio were analyzed by using analisis of variance (ANOVA) and further analysis by LSD if there were differences between treatments. The results of this experiment showed that spermatozoa quality Boer goat significantly reduced (p0,05) after separation with percoll gradient density centrifugation and swim up. Percentage spermatozoa X after percoll gradient density centrifugation was significantly higher (P0,05) compared to control and swim up. Meanwhile, the Y spermatozoa population was significantly higher (P0,05) after swim up treatment compared to percoll gradient density centrifugation and control. The percentage of sex ratio (male: female) after insemination from percoll gradient density centrifugation produced more female than male. On the other hand, insemination from swim up produced more male than female. Sex ratio produced from separation of percoll gradient density centrifugation, swim up was difference from control semen (P0,05). From this experiment, it was concluded that spermatozoa separation by percoll gradient density centrifugation and swim up can be used as one of the methods to separate X and Y spermatozoa and further can be applied to get preferred sex animals.
Adding fish meal into fermented moringa plus yellow corn in the diet does not affect performances but can recover breast yield of broiler Zulfan Zulfan; Allaily Allaily; Fitrah Khairi; Sri Jeksi; Andreansyah Andreansyah; Indri Saputri
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.74431

Abstract

Objective: This study aimed to investigate the use of fermented domesticated-type Moringa oleifera leaves (FDMOL) + yellow corn (YC) + rice bran (RB) + top mix (TM) with the provision of fish meal (FM) in replacement of partial commercial diet (CD) to broiler performances and carcass yields.Methods: The study used 100 broiler chicks, unsex, MB 90 performed into Completely Randomized Design (CRD) consisting of 5 treatments and 4 replicates. The trial diets were: MCR= 84.5% CD + 5% FDMOL + 5% YC + 5% RB + 0.5% TM (moringa+), MCF= 84.5% CD + 5% FDMOL + 5% YC + 5% FM + 0.5% TM (moringa++), C1= 100% CD, C2= 84.5% CD + 7.5% YC + 7.5% RB + 0.5% TM, and C3 = 84.5% CD + 5% YC + 5% RB + 5% FM + 0.5% TM.Results: Better adding fish meal into the FDMOL + YC rather than no fish meal in replacement of partial commercial diet to feed on the broilers. Most parameter performances and yields seemed not affected on broilers fed the FDMOL + YC base replacement diet, but the breast tended to reduce, then adding FM into FDMOL + YC relatively recovered this valuable part of cuts-up similar to the control.Conclusion: Adding fish meal into fermented domesticated Moringa oleifera plus yellow corn in the diet did not affect performances but could recover the breast yield of broiler compared to those fed the moringa-based diet without adding fish meal.
Co-Authors Aflesia Hagata Agasi, Satri Yusasra Al-kautsar Al-kautsar Allaily Allaily Allaily Allaily Allaily Allaily Allaily Allaily Allaily Allaily Andi Ananda Andi Saputra Andreansyah Andreansyah Ario Syah Putra Arismawan Arismawan Arismawan Arismawan Asmaul Husna Asmaul Husna Aswita, Evi Cut Aida Fitri Cut Aida Fitri Cut Aida Fitri Cut Aida Fitri Cut Aida Fitri Cut Aida Fitri Cut Aida Fitri Dasrul Dasrul Dasrul Dasrul Enten Juni Marlina Evi Aswita Evi Aswita Fitrah Khairi Fitrah Khairi Galih Ajie Pangestu Herawati Latif Herawati Latif Herawati Latif Herawati Latif Herawati Latif Herawati Latif Herawati Latif Herawati Latif Humaira Asra Iflijar Iflijar Ilham Ilham Indri Saputri Indri Saputri Iza Zulfiza Jaunda Fitra Khairul Murdani Lilyanda Fadilla Lukman Hakim M. A. Yaman M. Aman Yaman M. Aman Yaman M. Aman Yaman M. Aman Yaman M. Daud M.Aman Yaman Maghfira Daini Mudatsir Mudatsir Muhammad - Daud Muhammad Aman Yaman Muhammad Aman Yaman Muhammad Aman Yaman Muhammad Aman Yaman Muhammad Aman Yaman Muhammad Aman Yaman Muhammad Aman Yaman Muhammad Aman Yaman Muhammad Daud Muhammad Daud Muhammad Daud Muhammad Daud Muhammad Daud Muhammad Daud Muhammad Daud Muhammad Daud Muhammad Daud Muhammad Haris Munandar Muhammad Khomarun Zaman Muhammad Syairazi Murniati Murniati Nadia Nadia Nikita Anggraini Nur Ismira Nyakdin Nurganti Siapudan Rhaudatul Shaleha Rismanda Sari Rizki, Agam Siti Zharfa Rasyiqah Sri Jeksi Sri Jeksi Sri Wahyuni Sugito Sugito Sulaiman Ibrahim Syafriadi Syafriadi T. Fadrial Karmil Taufik Nazarullah Tri Hardianti P Unchu Maiwanda Vira Anatasha Wenny Ultaria Munthe Yadi Jufri Yeyen Safitri Yunasri Usman Zahrul Fuadi zulfikar zulfikar Zulfikar Zulfikar Zulfikar Zulfikar Zulfikar Zulfikar Zulfikar Zulfikar Zulfikar Zulfikar