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ISOLASI DAN IDENTIFIKASI KAPANG DAN KHAMIR PADA MEDIA PERTUMBUHAN MAGGOT Black Soldier Fly Shofa, Ghaitsa Salmalika; Badruzzaman, Deden Zamzam; Harlia, Ellin
Media Pertanian Vol 9, No 1 (2024): Media Pertanian
Publisher : Program Studi Agroteknologi Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/mp.v9i1.10585

Abstract

Metode biokonversi menggunakan organisme hidup yaitu maggot BSF (Black Soldier Fly) merupakan salah satu metode untuk mengurai bahan organik yang berasal dari limbah organik. Selain maggot terdapat mikroorganisme pengurai yang berperan dalam proses biokonversi, diantaranya yaitu kapang dan khamir. Penelitian ini bertujuan untuk mengetahui jumlah dan peran kapang serta khamir hasil isolasi dan identifikasi yang terdapat pada media pertumbuhan maggot yang berasal dari kombinasi feses sapi potong, endapan susu, dan sampah organik dapur. Metode yang digunakan dalam penelitian ini adalah metode deskriptif. Hasil penelitian menunjukkan bahwa rata-rata jumlah kapang dan khamir sebelum didegradasi oleh maggot lebih tinggi dibandingkan dengan setelah didegradasi oleh maggot. Jenis kapang yang teridentifikasi pada media pertumbuhan maggot adalah Aspergillus sp, Penicillium sp, Cladosporium sp, Rhizopus sp, Trichoderma sp, dan Mucor sp. Jenis khamir yang teridentifikasi pada media pertumbuhan maggot adalah Saccharomyces sp, Zygosaccharomyces sp, dan Trichosporon sp.The bioconversion method using living organisms, namely BSF (Black Soldier Fly) maggots, is one method to break down organic matter derived from organic waste. Besides maggots, there are parser microorganisms that play a role in the bioconversion process, among them are mold amd yeast. This research aims to determine the number and role of molds and yeasts isolated and identified on maggot growth media derived from a combination of beef cattle feces, dairy waste sludge and organic kitchen waste. The method used in this research is descriptive method. The results showed that the average number of molds and yeasts before degradation by maggot was higher than after degradation by maggot. The types of mold identified in the maggot growth media were Aspergillus sp, Penicillium sp, Cladosporium sp, Rhizopus sp, Trichoderma sp, and Mucor sp. The types of yeast identified in the maggot growth media were Saccharomyces sp, Zygosaccharomyces sp, and Trichosporon sp.
Population Density Levels Wight and Length of Maggot Black Soldier Flies (Hermetia Illucens) with Different Feed Formulation Khaerunnisa, Salma; Hidayati, Yuli Astuti; Harlia, Ellin
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 2 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i2.p111-121

Abstract

Black Soldier Fly (BSF) maggots are decomposing agents cultivated to convert organic materials. Both on-farm and off-farm livestock waste, as well as kitchen organic waste, are organic materials. Organic waste and its by-products can be degraded by BSF maggots into biomass rich in protein, which can be used as feed. This study aims to evaluate the effects of formulating dairy cow feces, milk sediment, and kitchen organic waste (KOW) as growth media on the population density, weight, and length of BSF maggots. The research method used is experimental with a Completely Randomized Design (CRD) involving four treatments and five replicates. The treatments include 100% KOW (P0), 50% KOW and 50% dairy cow feces (P1), 50% KOW and 50% milk sediment (P2), and 33.33% KOW, 33.33% dairy cow feces, and 33.33% milk sediment (P3). BSF maggot rearing was carried out for 20 days, from BSF eggs to the prepupa stage. The conclusion of this study was that the P2 formulation (dairy cow feces and kitchen organic waste) could increase the population density of BSF maggots, and the P3 formulation (dairy cow feces, milk sediment, and kitchen organic waste) could increase the weight and length of BSF maggots.
BIOAUGMENTATION OF MICROORGANISMS ON THE DECOMPOSITION OF A MIXTURE OF DAIRY CATTLE MANURE AND CHICKEN EXCRETA AS SOLID ORGANIC FERTILIZER Riyanto, Kirana Fairuza; Ridwan, Roni; Harlia, Ellin
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 25, No 3 (2025)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v25i3.61453

Abstract

Solid organic fertilizer efficiently utilizes livestock waste, with laying hen excreta providing the highest nitrogen (N) content among livestock manure. Combining laying hen excreta with dairy cow manure and fiber sources as a fertilizer base is expected to meet KEPMENTAN No. 261 of 2019 standards. To maintain essential nutrients (N, P, K), the fertilizer is enriched with Pseudomonas sp., Herbasirillum sp., and Streptomyces sp. This study aims to analyzing the effect of chicken excreta on macronutrient content (N, P, K), carbon (C) levels, C/N ratio, coliform presence, and microbial viability. The experiment followed a completely randomized design (CRD) with six treatments, namely P1: (95% Combination of DCF and FS + 5% Laying Hen Excreta), P2: (90% Combination of DCF and FS + 10% Laying Hen Excreta), P3: (85% Combination of DCF and FS +15% Laying Hen Excreta), MP1: (95% Combination of DCF and FS + 5% Laying Hen Excreta + 10% MK), MP2: (90% Combination of DCF and FS + 10% Laying Hen Excreta + 10% MK) and MP3: (85% Combination of DCF and FS + 15% Laying Hen Excreta + 10% MK). DCF (Dairy Cow Feces), FS (Fibre Source), MC (Microbial Consortium), SOF  (Solid Organic Fertilizer). Results showed that adding 15% laying hen excreta enriched with bacteria significantly increasing (P≤0.05) macronutrient content, viability, C/N ratio, and coliform presence but did not significantly affect carbon (C) content. 
Pemanfaatan POC Berbasis Limbah Ternak Untuk Penguatan Ketahanan Pangan Rumah Tangga Mandiri Di Desa Citali Kecamatan Pamulihan Kabupaten Sumedang Hidayati, Yuli Astuti; Harlia, Ellin; Marlina, Eulis Tanti; Badruzzaman, Deden Zamzam; Maulana, Yasin Pradana
Farmers : Journal of Community Services Vol 7, No 1 (2026): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v7i1.68095

Abstract

Desa Citali Kecamatan Pamulihan Kabupaten Sumedang merupakan desa dengan luas terkecil yaitu 1,51 km2 namun letak nya cukup strategis dipinggir jalan desa. Desa Citali terdiri dari 10 RW dan 28 RT, berdasarkan jenis kelamin jumlah penduduk laki-laki (2345 jiwa) dan perempuan (2328 jiwa) mempunyai rasio sama banyak. Sebagian besar masyarakatnya mempunyai mata pencaharian sebagai petani dan peternak. Di desa Citali terbentuk suatu lembaga yang mewadahi kegiatan para ibu-ibu dalam melakukan kegiatan pertanian dan peternakan yaitu yang dinamai dengan Kelompok Wanita Tani (KWT). Kegiatan pertanian dan peternakan selain menghasilkan produk utama, juga menghasilkan limbah. Kegiatan PkM ini memberikan inovasi penyuluhan dan pelatihan tentang pengolahan limbah peternakan dan pertanian menjadi POC dan dapat pemanfatannya untuk tanaman. Limbah ini diolah dengan metode pengolahan limbah terpadu menghasilkan pupuk organik cair (POC) dan dapat dimanfaatkan untuk memupuk tanaman pangan, sehingga dapat bermanfaat sebagai penguatan ketahanan pangan rumah tangga mandiri.Kata Kunci: limbah ternak, limbah pertanian, pengolahan terpadu, pupuk organik cair, ketahanan pangan 
Diseminasi Teknologi Pengolahan Limbah Ternak kepada Masyarakat Desa Cinangsi Kecamatan Cisitu Kabupaten Sumedang Marlina, Eulis Tanti; Hidayati, Yuli Astuti; Harlia, Ellin; Badruzzaman, Deden Zamzam; Maulana, Yasin Pradana
Farmers : Journal of Community Services Vol 7, No 1 (2026): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v7i1.67494

Abstract

Pengolahan limbah menjadi pupuk organik merupakan upaya yang dapat dilakukan masyakat peternak untuk merapkan konsep ekonomi sirkular di pedesaan.  Kepemilikan ternak di masyarakat Desa Cinangsi Cisitu Sumedang belum maksimal.  Beberapa alasan menjadi hambatan dalam pengolahan limbah menjadi pupuk organik adalah terbatasnya pengetahuan peternak mengenai potensi limbah ternak, baik potensi positif maupun negatif. Kegiatan penyuluhan di Desa Cinangsi dilaksanakan melalui kerjasama dengan Dinas Tenaga Kerja Kabupaten Sumedang yang bertujuan menciptakan peternak handal yang dapat mengelola industri peternakan dari hulu ke hilir dengan menerapkan konsep zero waste.   Pada kegiatan penyuluhan ini peternak diberi pengetahuan dampak limbah terhadap lingkungan dan maksimalisasi potensi limbah sebagai sumber bahan organik untuk dikonversi menjadi pupuk organik, pupuk organik padat (POP)  dan pupuk organik cair (POC), melalui proses pengomposan.  Metode penyuluhan melalui paparan teori dan diskusi serta praktik.  Peserta penyuluhan terdiri dari kelompok peternak yang mendapat bantuan ternak domba dari Dinas Tenaga Kerja Kabupaten Sumedang TA 2024.  Melalui pre test dan post test dapat disimpulkan terjadi peningkatan pengetahuan peternak mengenai dampak limbah terhadap lingkungan serta keterampilan dalam mengolah limbah ternak menjadi pupuk organik. 
Pengaruh Campuran Feses Sapi Potong dan Feses Kuda Pada Proses Pengomposan Terhadap Kualitas Kompos Hidayati, Yuli Astuti; Marlina, Eulis Tanti; Benito AK, Tb; Harlia, Ellin
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 13 No 6 (2010): Mei 2010
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.046 KB) | DOI: 10.22437/jiiip.v0i0.121

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This  study  aims  to  determine  compost  quality  (content  of   N,  P2O5, K2O)  generated  from  various mixtures  of  beef  cattle  feces  and  the  feces  of  horses.  The method    used    in  this  study  is  the  experimental method in the laboratory using a completely randomized design with 3 treatments, ie P1 = C / N ratio of 25, P2  =  C  / N  ratio  of  30  and  P3  =  C  / N  ratio  of  35  and  replications  6  times.  To  determine  the  effect  of treatments, data were analyzed with ANOVA and Duncan test. The results showed that the mixture of feces of beef  cattle and horse  feces with various C  / N  ratio did not  significantly affect  the quality of  compost. N content  in  compost  produced =  0.7867  to  0.8000%, P2O5  content =  0.5883  to  0.6000%, K2O =  0.5733  to 0.5883%
Utilization of sheep dung and rice straw with indigenous microbial agent to optimize vermicompost production and quality YULI ASTUTI HIDAYATI; SITI NURACHMA; DEDEN ZAMZAM BADRUZZAMAN; EULIS TANTI MARLINA; ELLIN HARLIA
Biodiversitas Journal of Biological Diversity Vol. 22 No. 12 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221227

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Abstract. Hidayati YA, Nurachma S, Badruzzaman DZ, Marlina ET, Harlia E. 2021. Utilization of sheep dung and rice straw with indigenous microbial agent to optimize vermicompost production and quality. Biodiversitas 22: 5445-5451. Sheep dung is a useful by product that can be potentially processed into more useful products with minimal pollution. Vermicomposting is a waste processing method that produces Solid Organic Fertilizer and worm biomass as the raw materials for drugs and cosmetics. This research aimed to utilize the role of indigenous fungi and bacteria to determine the influence of C/N ratio from sheep dung and rice straw mixture on vermicompost quality (N, P, K, Ca, Mg). This experimental research was conducted in a Completely Randomized Design with three treatments (T1: C/N ratio of 25; T2: C/N ratio 30; T3: C/N ratio 35) and six replicates for each treatment. Data so obtained were analyzed with ANOVA and Duncan's Multiple Range Test. The results showed that C/N ratio had a noticeable influence on vermicompost quality. C/N ratio 30 produced the highest vermicompost quality (N: 1.11 ± 023381%; P: 0.56 ± 0.010328 %; K: 0.51 ± 0.021369 %; Ca: 0.33 ± 0.0248 %; Mg: 0.14 ± 0.0228%).
Microbial quality and macronutrient of vermicompost produced by Eisenia fetida in dairy wastewater solids EULIS TANTI MARLINA; YULI ASTUTI HIDAYATI; ELLIN HARLIA; DEDEN ZAMZAM BADRUZZAMAN; NAOMI MEYNADHEA; NUR ARISTAWIDYA RAHAYU
Biodiversitas Journal of Biological Diversity Vol. 25 No. 6 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250643

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Abstract. Marlina ET, Hidayati YA, Harlia E, Badruzzaman DZ, Meynadhea N, Rahayu NA. 2024. Microbial quality and macronutrient of vermicompost produced by Eisenia fetida in dairy wastewater solids. Biodiversitas 25: 2729-2737. Dairy Wastewater Solids (DWS) contain enough nutrients for the growth of earthworms and utilize the nutritional requirements of livestock waste for growth. The growth of earthworms affects the quality of vermicompost or castings that are useful as organic fertilizers. This research aimed to determine the growth of Eisenia fertida using DWS as a growth medium, and its effect on the quality of vermicompost produced. The organic material decomposed in DWS by indigenous microorganisms and earthworms can convert DWS into organic fertilizer through composting. Rice straw was added as a carbon source to obtain the ideal C/N ratio for the growth of decomposing microorganisms. Good growth of decomposer microorganisms can increase the availability of nutrients in DWS as a source of plant nutrition. E. fetida was used in vermicomposting for 14 days after the initial decomposition phase had passed. This research used the experimental method of Completely Randomized Design (CRD) with three stocking density treatments (T1: 6.6 g/L; T2: 13.3 g/L; T3: 20.0 g/L ) and six replications. The results showed that higher stocking density promoted an increase in the biomass weight of earthworms. Various indigenous microorganisms act as decomposers and work with earthworms to convert organic matter into inorganic matter as a plant nutrient. The stocking density treatment had no significant effect on vermicompost production, total nitrogen content, and P2O5 vermicompost but had a significant effect on K2O, Ca, Mg, population of Nitrogen-Fixing Bacteria (NFB) and Phosphate-Solubilizing Bacteria (PSB). Vermicomposting, DWS, and rice straw mixture produced vermicompost with high functional microbial and macronutrient concentrations.
Isolation and cellulolytic activity of bacteria from beef cattle feces using rice straw as enrichment substrate EULIS TANTI MARLINA; YULI ASTUTI HIDAYATI; ELLIN HARLIA; YASIN PRADANA MAULANA; GINA CHYNTHIA KAMARUDIN PUTERI
Biodiversitas Journal of Biological Diversity Vol. 27 No. 6 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270617

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Abstract. Marlina ET, Hidayati YA, Harlia E, Maulana YP, Puteri GCK. 2026. Isolation and cellulolytic activity of bacteria from beef cattle feces using rice straw as enrichment substrate. Biodiversitas 27 (6): d270617. https://doi.org/10.13057/biodiv/d270617. Beef cattle (Bos indicus/taurus) feces contain diverse microbial communities with potential roles in lignocellulosic biomass transformation. This study aimed to evaluate the initial decomposition of rice straw and beef cattle feces, using this information as a basis for isolating and preliminarily identifying cellulolytic bacteria from beef cattle waste. Rice straw was mixed with beef cattle feces to initiate decomposition for 7 days. Changes in chemical composition and structure were assessed using fiber fraction analysis and Scanning Electron Microscopy (SEM). The addition of cattle feces reduced Acid Detergent Fiber (ADF) from 51.36% to 42.31%, Neutral Detergent Fiber (NDF) from 72.08% to 60.82%, lignin from 12.89% to 7.40%, cellulose from 38.47% to 34.91%, and hemicellulose from 20.72% to 18.51%. SEM showed increased surface roughness and fiber degradation (loosening and fragmentation), with structural changes that can be attributed to microbial activity. Based on this finding, microbial enrichment was carried out with cattle feces and rice straw, with the addition of molasses as a nutrient source for microbial growth. Seven bacterial isolates were obtained, among which three (LS-6, LS-11 and LS-14) exhibited the highest cellulolytic activity on Carboxy-Methyl Cellulose (CMC) agar and  dinitrosalicylic acid (DNS). Molecular level identification of these isolates through 16S rRNA gene sequence revealed that these isolates were closely affiliated with Bacillus safensis and Bacillus pumilus group. The results suggest that the isolates exhibit cellulolytic activity under the test conditions and may contribute to lignocellulose degradation. This study provides a first step for further research on cellulolytic bacteria from beef cattle feces for the effective use of lignocellulosic biomass.
Penyuluhan dan Pelatihan Pengolahan Sampah Dapur Organik di Desa Jelegong Kecamatan Rancaekek Kabupaten Bandung HIDAYATI, YULI ASTUTI; Harlia, Ellin; Marlina, Eulis Tanti; Badruzzaman, Deden Zamzam; Maulana, Yasin Pradana
Farmers : Journal of Community Services Vol 7, No 2 (2026): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v7i2.72008

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Desa Jelegong merupakan salah satu desa yang berada di wilayah administratif Kecamatan Kutawaringin, Kabupaten Bandung, Provinsi Jawa Barat. Wilayah Administrasi terbagi ke dalam 4 dusun, 19 RW, dan 73 RT. Desa ini termasuk dalam kawasan pedesaan yang memiliki potensi sumber daya alam dan sosial yang cukup berkembang. Desa ini memiliki iklim yang relatif sejuk dan mendukung aktivitas pertanian serta pemukiman masyarakat. Salahsatu Lembaga yang ada di desa Jelegong adalah  Pemberdayaan dan Kesejahteraan Keluarga (PKK), merupakan organisasi kemasyarakatan yang berfokus pada pemberdayaan keluarga sebagai unit terkecil dalam masyarakat. Gerakan ini bertujuan untuk meningkatkan kualitas hidup masyarakat melalui berbagai program yang mencakup aspek kesehatan, pendidikan, ekonomi, dan lingkungan. Kelompok PKK ini terdiri dari ketua, sekretaris, bendahara dan POKJA-PKJA, serta anggotanya merupakan ibu-ibu kader sebagai penggerak PKK. Berdasarkan tujuan PKK untuk meningkatkan kualitas hidup masyarakat dari aspek lingkungan, Salahsatu kondisi lingkungan yang perlu diperhatikan adalah menumpuknya timbulan sampah yang berasal dari dapur rumahtangga masyarakat desa Jelegong. Kegiatan PkM ini bertujuan memberikan pengetahuan dan ketrampilan dalam mengolah limbah dapur organik diolah menggunakan metode pengomposan menjadi kompos dan dapat dimanfaatkan sebagai pupuk organik