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EFEK STUNTING TERHADAP KADAR GLUTATION SEBAGAI PENANDA STRES OKSIDATIF PADA ANAK Rahmanita, Hania Asmarani; Gunarti, Dwirini Retno; Andayani, Diyah Eka
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 7 No 3 (2024): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Stunting merupakan masalah kesehatan yang dikenal luas dan merupakan masalah malnutrisi yang paling umum terjadi di dunia. Pada tahun 2022, ada sekitar 148 juta atau 22,3% anak di bawah usia lima tahun di seluruh dunia mengalami stunting. Kekurangan gizi kronis dan infeksi berulang. pada anak stunting dapat menyebabkan kondisi stres oksidatif, yaitu suatu fenomena yang disebabkan oleh ketidakseimbangan antara prooksidan dan antioksidan yang terjadi di dalam sel tubuh. Glutation (GSH) merupakan antioksidan endogen non enzimatik dengan jumlah terbesar di dalam tubuh yang merupakan tripeptida, terdiri dari glutamat, sistein, dan glisin. Pada anak-anak terutama balita, kadar GSH sangat dipengaruhi oleh faktor nutrisi sehingga kondisi stunting dapat menyebabkan perubahan kadar GSH. Tinjauan literatur ini bertujuan untuk menjelaskan efek dari stunting terhadap salah satu penanda stres oksidatif yaitu antioksidan glutation (GSH) pada anak. Hasil dari penelusuran menunjukkan bahwa terdapat perubahan kadar GSH pada anak stunting yang berkaitan dengan perubahan fungsi tubuh dalam jangka pendek maupun jangka panjang yang dapat meningkatkan morbiditas dan mortalitas. Kata kunci : antioksidan, glutation, GSH, stres oksidatif, stunting DOI : 10.35990/mk.v7n3.p273-284

PERAN FLAVONOID TERHADAP TNF ALPHA PADA ENDOMETRIOSIS Wardani, Putri Kusuma; Gunarti, Dwirini Retno; Wulandari, Yohannessa
JURNAL DARMA AGUNG Vol 31 No 3 (2023): JUNI
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Darma Agung (LPPM_UDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46930/ojsuda.v31i3.3454

Endometriosis adalah kondisi yang ditandai oleh pertumbuhan jaringan endometrium di luar rahim, yang dapat menyebabkan gejala nyeri panggul dan gangguan reproduksi. Penelitian sebelumnya telah menunjukkan bahwa tingkat sitokin pro-inflamasi, seperti TNF alpha, meningkat pada endometriosis. Flavonoid, senyawa yang ditemukan dalam berbagai tanaman, telah menunjukkan potensi antiinflamasi dan dapat mempengaruhi ekspresi TNF alpha. Oleh karena itu, penelitian ini bertujuan untuk memahami peran flavonoid dalam mengatur TNF alpha pada endometriosis. Tujuan penelitian ini adalah untuk menginvestigasi peran flavonoid terhadap ekspresi TNF alpha pada endometriosis. Penelitian ini menggunakan metode penelitian kualitatif untuk mendapatkan pemahaman mendalam tentang peran flavonoid dalam hubungannya dengan TNF alpha pada endometriosis. Data dikumpulkan melalui studi kepustakaan yang diperoleh melalui Google Schoolar dan PubMed. Hasil penelitian menunjukan bahwa peran flavonoid memiliki peran dalam mengatur produksi dan aktivitas TNF alpha dalam tubuh. Flavonoid dapat menghambat produksi TNF alpha di tingkat sel dengan mengganggu jalur sinyal yang terlibat dalam regulasi produksi sitokin ini. Sehingga dapat disimpulkan bahwa flavonoid memiliki peran penting dalam endometriosis melalui mekanisme anti-inflamasi dan antiangiogenesis sebagai penghambatan ekspresi dan aktivitas TNF alpha, pengendalian pertumbuhan jaringan endometrium, dan regulasi sistem imun.

Peran Pemeriksaan Malondialdehid sebagai Penanda Stres Oksidatif pada Anak Stunting: Tinjauan Sistematik Tarigan, Ananda Putri; Gunarti, Dwirini Retno; Sunardi, Diana
Majalah Kedokteran Indonesia Vol 74 No 4 (2024): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.74.4-2024-1256

Introduction: Stunting is a form of chronic malnutrition which is still a nutritional problem in Indonesia. Stunting is characterized by a decrease in insulin-like growth factor-1, closely related to the release of excess reactive oxygen species that trigger oxidative stress. An increase in reactive oxygen species that bind to membrane lipids produces lipid peroxidase, which impacts cell damage. The research aims to obtain information regarding the role of malondialdehyde measurement as an early marker and a prognostic determinant of oxidative stress in protein-energy malnutrition children, such as stunted and marasmic.Method: Systematic literature was conducted with published works journal searches from 23rd – 30th November 2023, relevant keywords related to the research topic in ScienceDirect, PubMed, and Cochrane databases using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis 2020 method. The quality of selected studies was evaluated using The Joanna Briggs Institute Critical Appraisal Tools. The research protocol was registered at PROSPERO (CRD42024568540).Results: The data obtained were five articles from cross-sectional studies with the results had increased oxidative stress biomarkers, including malondialdehyde, which is significantly higher in protein-energy malnutrition children, such as stunted and marasmic, than in healthy children (p less than 0.05).Conclusion: The malondialdehyde levels as biomarkers of oxidative stress were found to be high in stunted children.

The Effect of Giving One Egg Per Day on Stunted Children Aged 2-5 Years in Buton Regency on Zinc Levels Saputro, Alvreyna Cherienda Laviashna; Gunarti, Dwirini Retno
Archives of Pediatric Gastroenterology, Hepatology, and Nutrition Vol. 4 No. 2 (2025): APGHN Vol. 4 No. 2 May 2025
Publisher : The Indonesian Society of Pediatric Gastroenterology, Hepatology, and Nutrition

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58427/apghn.4.2.2025.62-72

Background: Despite can be prevented with good nutrition, average prevalence of stunting in Indonesia remains high at 30.8%. The effects of stunting are detrimental to the individual and Indonesia's human resources. Stunted children had low blood zinc levels which disrupted enzyme and antioxidant activity, as well as bone growth processes and bone homeostasis. Eggs that contain zinc and have high bioavailability are expected to be able to increase low blood zinc levels. This study was designed to examine the impact of routine egg consumption on zinc levels in stunted children. Methods: This experimental quantitative study was conducted in the Siontapina Health Center, Buton Regency, Southeast Sulawesi. and included 22 participants which divided into intervention and control groups. The intervention was carried out by health workers giving one egg per day for 30 days with the same type of cooking per day to the participants. Zinc levels were assessed using a spectrophotometer and analyzed using statistical software. Result: The results showed a significant difference between the two groups (p <0.05). The average zinc level in the intervention group was 718.8133 ug/dL, which was higher than the control group (143.4536 ug/dL). Thus, giving 1 egg a day for 30 days caused a significant change in blood zinc levels in stunted children aged 2-5 years in Buton Regency. Conclusion: Stunted children supplemented with one egg daily for 30 days had significantly higher zinc levels than unsupplemented children.

Studi awal: Identifikasi Keragaman Gen Bakteri Saliva pada Wanita Usia Lanjut Menggunakan Teknologi 16S Ribosome Sequencing Oxford Nanopore Adhiyanto, Chris; Hendarmin, Laifa A; Harriyati, Zeti; Suwarsono, Erike A. Suwarsono A; Suri, Auliyani Andam; Nugraha, Yudhi; Gunarti, Dwirini Retno
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 7, No 1 (2025)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.46752

The elderly are a population particularly susceptible to diseases resulting from the decline in immune system function. The mouth is one of the areas of the body that is the entry point for microorganisms into the body. It is known that the oral microbiome greatly affects oral and systemic health. The oral bacterial profile is important to understand its role in maintaining health and causing disease. Therefore, maintaining oral health is very important, especially in our study of the elderly. Utilizing advanced sequencing techniques such as Oxford Nanopore Technology (ONT) offers the opportunity to reveal bacterial diversity comprehensively. This study aims to identify the oral microbiome in a group of elderly women. Knowledge of the oral microbiota profile could help spread awareness of the benefits of maintaining good dental hygiene, especially in older adults. Methods: Seven elderly housewives had their unstimulated saliva samples taken in the morning and kept at -80°C. The phenol-chloroform technique was used to isolate the DNA, and a Qubit Fluorometer was used to measure purity and concentration. By employing ONT for 16S rRNA sequencing and Epi2Me software for analysis, taxonomic mapping of the bacterial diversity was accomplished. Results: According to the taxonomic analysis, Streptococcus and Veillonella were the most common genera in the samples. It was common to find species like Veillonella parvula, Veillonella atypica, Streptococcus mitis, and Streptococcus rubneri. These genera contributed to the dynamics of oral biofilms by exhibiting complex ecological interactions. With advanced sequencing technologies, this work highlights the diversity of oral bacteria in elderly women in Indonesia while providing a new perspective. Notwithstanding a few limitations, these findings provide insight into the ecological dynamics of the oral microbiota, potentially leading to clinical applications in the treatment and prevention of disease.

Isolation and Purification of Breast Milk Folate Binding Protein: Salting-Out and Chromatography Techniques Saleh, Mgs. M. Irsan; Subandrate, Subandrate; Gunarti, Dwirini Retno; Hermansyah, Hermansyah; Hafy, Zen; Kesuma, Yudianita; Sadikin, Mohamad
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.11303

ABSTRACT. Folate binding protein (FBP) is a protein in breast milk that plays a role in the regulation and bioavailability of folic acid. In contrast to cow's milk FBP, information about breast milk FBP is still limited. This research aims to determine the isolation and purification methods of breast milk FBP and the molecular weight of breast milk FBP. The sample in this study was 1000 mL of breast milk. Breast milk was prepared in several stages to yield whey. Isolation and purification of FBP from whey were carried out in stages, salting-out, ion exchange chromatography, and affinity chromatography. Whey salting-out with 95% saturation of ammonium sulfate could precipitate folate-binding proteins. This precipitate showed three peaks on DEAE chromatography. Peak II DEAE 95% was thought to be a negatively charged folate-binding protein. Peak II DEAE 95% also showed the presence of two peaks on affinity chromatography. It was believed that Peak II AF 95% was a pure folate-binding protein. Peak II AF 95% showed the presence of a single band on SDS-PAGE and western blot. This indicated that the folate-binding protein was 100% pure. FBP can be isolated from breast milk by the salting-out method using 95% ammonium sulfate, DEAE chromatography, and affinity chromatography. FBP from breast milk has a molecular weight of approximately 37 kDa. The final level of FBP isolated from breast milk is approximately 0.022 mg/mL. The successful isolation of FBP from breast milk provides an opportunity to use it to understand the clinical role of FBP in increasing folic acid levels in both breast milk and infant serum, as well as to develop methods for determining folic acid levels in these fluids. Keywords: Breast milk, folate binding protein, isolation, purification, molecular weight

Superoxide dismutase (SOD) activity in stunted children: Review article Badrya, Leily; Retno Gunarti, Dwirini; Wulandari, Yohannessa
Science Midwifery Vol 12 No 2 (2024): June: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v12i2.1581

This literature review aims to provide an overview of superoxide dismutase (SOD) activity in stunted children. Method is a literature search was done regarding superoxide dismutase activity in stunting. The literature search utilized PubMed to search for published articles, including clinical trials, case reports, and review articles between 2011 and 2023. The result of stunting is a type of chronic malnutrition caused by long-term malnutrition in children. Malnutrition is the cause of 45% of all deaths in children aged <5 years. Deficiency of macronutrients, especially protein and amino acids, especially leucine and arginine in stunting, can cause a decrease in mTORC1 activation, as a pathway in the body's protein synthesis, especially enzymatic synthesis. Disruption of the mTORC1 pathway due to stunting causes a decrease in the synthesis of enzymatic endogenous antioxidants, especially superoxide dismutase (SOD), followed by an increase in free radical molecules in the body. An imbalance between superoxide dismutase (SOD) and an increase in free radicals in stunted children can create short-term impacts, such as recurrent acute infections. There can also be long-term impacts, namely non-communicable diseases, such as obesity, heart disease, and diabetes mellitus in the future. Conclusion: based on this literature review, it can be concluded that there is a decrease in the synthesis and activity of superoxide dismutase (SOD) found in stunted children.

Studi Awal: Identifikasi Keragaman Bakteri Saliva Wanita Usia Lanjut Menggunakan Teknologi 16S rRNA Sequencing Oxford Nanopore Adhiyanto., Ph.D, Chris; Hendarmin, Laifa A.; Harriyati, Zeti; Suwarsono, Erike A.; Suri , Auliyani Andam; Nugraha, Yudhi; Gunarti, Dwirini Retno
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol. 7 No. 1 (2025)
Publisher : Pharmaceutical and Biomedical Sciences Journal (PBSJ)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.49111

The elderly are a population particularly susceptible to diseases resulting from the decline in immune system function. The mouth is one of the areas of the body that is the entry point for microorganisms into the body. It is known that the oral microbiome greatly affects oral and systemic health. The oral bacterial profile is important to understand its role in maintaining health and causing disease. Therefore, maintaining oral health is very important, especially in our study of the elderly. Utilizing advanced sequencing techniques such as Oxford Nanopore Technology (ONT) offers the opportunity to reveal bacterial diversity comprehensively. This study aims to identify the oral microbiome in a group of elderly women. Knowledge of the oral microbiota profile could help spread awareness of the benefits of maintaining good dental hygiene, especially in older adults. Methods: Seven elderly housewives had their unstimulated saliva samples taken in the morning and kept at -80°C. The phenol-chloroform technique was used to isolate the DNA, and a Qubit Fluorometer was used to measure purity and concentration. By employing ONT for 16S rRNA sequencing and Epi2Me software for analysis, taxonomic mapping of the bacterial diversity was accomplished. Results: According to the taxonomic analysis, Streptococcus and Veillonella were the most common genera in the samples. It was common to find species like Veillonella parvula, Veillonella atypica, Streptococcus mitis, and Streptococcus rubneri. These genera contributed to the dynamics of oral biofilms by exhibiting complex ecological interactions. With advanced sequencing technologies, this work highlights the diversity of oral bacteria in elderly women in Indonesia while providing a new perspective. Notwithstanding a few limitations, these findings provide insight into the ecological dynamics of the oral microbiota, potentially leading to clinical applications in the treatment and prevention of disease.

Purification of total IgG from sars-cov-2 convalescent serum Lusinanto, Arfat; Gantini, Ria Syafitri Evi; Gunarti, Dwirini Retno; Sadikin, Mohamad
Acta Biochimica Indonesiana Vol. 8 No. 2 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.203

Background: Convalescent plasma contains neutralizing antibodies against SARS-CoV-2 but also potentially harmful inflammatory cytokines. Purified immunoglobulin G fractions offer a safer therapeutic alternative by concentrating antibodies while removing inflammatory mediators. Objective: To establish a systematic protocol for purifying total IgG from SARS-CoV-2 convalescent serum using sequential chromatographic techniques. Methods: Serum from 90 recovered donors was pooled into three independent samples. Purification employed four sequential steps: ammonium sulfate precipitation (50% saturation), Sephadex G-100 size-exclusion chromatography, DEAE-Cellulose ion-exchange chromatography, and Protein A affinity chromatography. Purity was assessed by native polyacrylamide gel electrophoresis and radial immunodiffusion. Results: Starting from serum with 19.68 ± 7.27 mg/mL IgG and 110.47 ± 11.99 mg/mL total protein, the four-step purification yielded final IgG concentration of 1.14 ± 0.70 mg/mL with 1.19 ± 0.16 mg/mL total protein. This achieved 6.3-fold purification with purity ratio of 1.01 ± 0.38 and 5.8% recovery. Native PAGE confirmed high purity with a single IgG band. Conclusion: Sequential chromatography successfully purified total IgG from convalescent serum, providing a laboratory-scale method for preparing safer immunoglobulin therapeutics.

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