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Journal : Rumphius Pattimura Biological Journal

DNA Barcoding Application Using CYTB (Cytochrome-B) Marks for Authentication of Patin Fish (Pangasius sp.) Products in Surabaya Area Aulia, Wina Intan Nur; Setiawan, Edwin; Triwibowo, Radestya; Saputro, Triono Bagus; Hidayati, Dewi; Nurhayati, Awik Puji Dyah; Ridho, Muhammad Ainur Rosyid
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p079-092

Abstract

DNA barcoding is a practical and accurate molecular technique for identifying processed fish products that lack morphological characteristics. However, its application is limited by the availability of reference databases, DNA quality, and primer suitability. This study aims to determine the genetic identity of pangasius catfish (Pangasius sp.) marketed in Surabaya using the Cytochrome b (CytB) gene and to evaluate PCR-RFLP as an alternative to sequencing. A total of twenty samples (fresh and frozen fillets) were analyzed through DNA extraction and PCR amplification of the CytB gene, followed by in silico and in vitro analyses.The results show that CytB demonstrates high amplification success and provides sufficient resolution for species-level identification based on Neighbor-Joining analysis with the Kimura 2-parameter model. The study confirms that the samples were genetically identified as Pangasius pangasius and Pangasius hypophthalmus. This research highlights the novelty of integrating in silico prediction with laboratory validation to improve marker evaluation in DNA barcoding studies. Furthermore, it demonstrates that CytB-based PCR-RFLP is a rapid and cost-effective alternative to sequencing for detecting species mislabeling. Overall, the CytB gene is proven to be a reliable molecular marker for species authentication, supporting traceability systems and strengthening seafood authenticity monitoring in commercial markets.
Co-Authors A, Aunurohim Abdul Wahab Abdul Wahab Adik Mayang Indiani Adik Mayang Indiani Afrilya Dina Salsabila Agus Wahyudi Agus Wahyudi Ahmad Ardiansyah, Defan Agmu Alfani Raziful Dwi Ersandy Alfin Desta Pramaysella Alifia Cahyaning Putri Anies Wijayanti Anies Wijayanti Anto Budiharjo Arina Tri Lunggani Aulia Istighfarotunnisa Aulia, Wina Intan Nur Aunurohim Aunurohim Awik Pudji Diah Nurhayati Awik Pudji Diah Nurhayati Awik Puji Dyah Nurhayati Budiharjo Anto CATUR RIANI Christoforus Kalo Bello Puyanggana Danang Samudra David J. Marshall David Relex Devi Pramesti Wulandari Dewantoro, Giwang Dewi Hidayati Didit Prasetyo Dini Adni Navastara, Dini Adni Dini Ermavitalini Dyah Wulandari Elshinta Riantica Enny Zulaika Enny Zulaika F, Fauziah Faizah Nur Shabrina Farid Kamal Muzaki Farikha, Siti Lutfiatul Geoloveriana Esasi Hartiwiningsih Hartiwiningsih Hellend , Arbend Ficasso Van Hermanto, Michael Einstein Hermawan Hermawan Hermawan Indah Trisnawati Dwi Tjahjaningrum Iska Desmawati Kamila Islamiati Kharisma Armida Ulayya Kristianto Kristianto Lanny Kartikasari Lissa Rosdiana Lissa Rosdiana Mahendra, Januar Rahadian Maitsa Adzro Fania Marita Ika Joesidawati Michael Einstein Hermanto Millah, Ro’iqotul Mulyadi, Yeyes Nadhila, Auliya Rahma Nadila Wulan Cahyani Natanael Hari Wijaya Ni Wayan Ni Wayan Noor Nailis Sa'adah Noor Nailis Saadah Noor Nailis Saa’dah Noor Nailis Sa’adah Nova Maulidina Ashari Nova Maulidina Ashuri Nurlita Abdulgani Nurlita Abdulgani Nurul Jadid Prayogo, Fitra Adi Raden Mohamad Herdian Bhakti Rahmadani, Syakirah Shafwah Ratna Rintaningrum Riani Catur Ridho, Muhammad Ainur Rosyid Ritonga, Putri Chairani Rony Irawanto S, Suwarsih Sa'adah, Noor Nailis Saptarini, Dian Setyawan Wibisono Setyo Nugroho Shabrina Syifa Ghaissani Shovitri, Maya Sintya Agustina Eka Putri Sukma, Nur Awaliyah Mentari Syahroni, Nur Triono Bagus Saputro, Triono Bagus Triwibowo, Radestya Yanuar Ahmad Zufar Azzaria Azmi Zuwanita, Cyntya Sri