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Effect of Media and Plant Growth Regulator on Proliferation of Somatic Embryogenic Callus of Oil Palm (Elaeis gueenesis Jacq.) Sulaksono, Gogoh; Hasmeda, Mery; Hanum, Laila; Wendra, Fahmi; Santika, Baitha
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.4234

Abstract

The Pisifera type oil palm is an important palm variety and mostly used as a pollen source to produce Tenera commercial hybrid varieties. This research on proliferating fertile Pisifera oil palm callus using somatic embryogenesis method was conducted at the Tissue Culture Laboratory of PT. Sampoerna Agro, Tbk from February to August 2021 to find out the optimum basal media and the concentration of growth regulators on callus proliferation. The planting material was the primary callus from the previous research. This study used a factorial Completely Randomized Design with two factors. The first factor involved two types of basal media: MS and Y3, and the second factor consisted of different concentrations of growth regulators (PGR). All treatments were repeated three times. The results showed that the use of MS media supplemented with 6 mg/l NAA and 0.5 mg/l 2,4-D was the most effective treatment for the proliferation of embryogenic callus in term of callus initiation time, the capacity of primary callus formation, primary calli morphology and proliferation, and callus fresh weight.
Peningkatan Toleransi Rendaman Padi Lokal Rawa Lebak dengan Introgresi Gen Sub1 ,, Gusmiatun; Suwignyo, Rujito A.; Hasmeda, Mery; Wijaya, Andi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.378 KB) | DOI: 10.24831/jai.v43i2.10409

Abstract

ABSTRACTOne of efforts to increase rice production at swamp area which is frequently flooded is the use of submergence tolerant rice varieties. The objectives of this research was to improve plant tolerance after submergence stress for 14 days in BC1F1 of crosses between local superior varieties and Sub1 gen donor (FR13A). The studies were carried out in two stages: 1) Crossing local superior varieties, i.e., Pegagan (Pgn), Siam (Sm), Payak Selimbuk (Pys), dan Pelita Rampak (Plr) to submergence tolerant variety FR13A; and 2) submergence tolerance evaluation of the progeny. The first study was conducted at green house of Graduate Study Sriwijaya University, Palembang. The second study was carried out at field experiment of Central Research and Development of Agricultural Biotechnology and Genetic Resources in Bogor. All studies were conducted from March 2011 until March 2013. The first study was the introgression of Sub1 gene into local genotypes Payak Selimbuk, Pegagan, Siam and Pelita Rampak from FR13A. The second study was evaluation of BC1F1 plant performance after submergence treatment for 14 days. The results showed that introgression of Sub1 gene into local varieties improved submergence tolerance as indicated by higher percentage of survived plants, lower decrease of plant dry weight, and lower decrease of grain weight per panicle compared to parents.Keywords: Sub1 gene, local swamp rice, submergence tolerant
Priming Benih Padi (Oryza sativa L.) dengan Zn untuk Meningkatkan Vigor Bibit pada Cekaman Terendam Sulaiman, Sulaiman; Suwignyo, Rujito Agus; Hasmeda, Mery; Wijaya, Andi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 1 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.525 KB) | DOI: 10.24831/jai.v44i1.12486

Abstract

Seed priming has been widely used to improve seedling vigor of several crops  such as corn, wheat, and rice. Seed priming with zinc to increase seedling tolerance on submergence stress had never been evaluated. The objective of this research was to identify agronomical and physiological characteristics of several rice genotypes during seedling stage at submergence stress following zinc priming treatment. This research used factorial randomized block design with three replications. The first factor was rice genotypes and second factor was zinc solution of ZnSO4.7H2O. Seeds were soaked with Zn solution at different concentrations namely 0, 2.5, dan 5.0 mM respectively for 24 hours. After that the seeds were germinated on soil media in side the plastic box. Fourteen days-old seedling were submerged in water for 5 days and then return to normal condition for recovery. Parameters being observed included seedling dry weight, shoot carbohydrate, and leaves chlorophyl which were measured after 10 days of recovery. Seedling height was measured once a week from 2nd to 4th week. Results showed that intolerant genotypes to submergence stress grew faster but seedling dry weight, shoot carbohydrate and leaves chlorophyll content were lower compared to those tolerant genotypes. Local and national varieties which were not tolerant to submergence stress could be improved by priming treatment with 5.0mM zinc.Keywords: abiotic-stress, seeds- priming
Pengaruh Panjang Hari, Asam Indol Asetat, dan Fosfor terhadap Tanaman Kedelai dan Kualitas Benih dalam Penyimpanan Hartawan, Rudi; Djafar, Zainal Ridho; Negara, Zaidan Panji; Hasmeda, Mery; Zulkarnain, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 1 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.34 KB) | DOI: 10.24831/jai.v39i1.13181

Abstract

The objective of this study was to investigate the effect of photoperiod, indole acetic acid (IAA), and phosphorus on soybean plant growth and seed quality during storage. The trial was carried out at Sebapo Experimental Station, Jambi, Center for Post Harvest Research and Development, The Ministry of Agriculture and Center for Forest Research and Development, The Ministry of Forestry, Bogor from November 2009 until June 2010. Split-split plot design was used consisted of three factors, i.e. photoperiods (12 and 14 hours 54 minutes) as the main plot, concentrations of IAA (0, 75, and 150 ppm) as subplot, and dosages of phosphorus (0, 60, and 120 kg P2O5 ha-1) as subplot. The result showed that photoperiod, IAA, and phosphorus application had significantly improved production and seed quality. The path analysis showed that the weight of 1,000 seeds affected other variables. The seed protein content has the largest path coefficient compared to other variables. The treatment of mother plant with 14 hours 54 minutes photoperiod combined with IAA of 150 ppm and P2O5 of 120 kg ha-1 resulted in the highest seed quality during 90 days of storage in room temperature.
PENGEMBANGAN PROGRAM PEKARANGAN PANGAN LESTARI (P2L) BERBASIS TEKNOLOGI HIDROPONIK VERTIKULTUR UNTUK MENDUKUNG KEMANDIRIAN PANGAN KELUARGA DI DESA TANJUNG BARU KECAMATAN INDRALAYA UTARA Irmawati, Irmawati; Rusdan, Risna; Amiros, Nabilah; Fadhilah Rizar, Fitra; Purnama Ria, Rofiqoh; Hasmeda, Mery
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 8, No 12 (2025): MARTABE : JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v8i12.%p

Abstract

Program Pekarangan Pangan Lestari (P2L) bertujuan meningkatkan kemandirian pangan keluarga melalui pemanfaatan lahan pekarangan secara produktif dan berkelanjutan. Kegiatan pengabdian ini dilaksanakan di Desa Tanjung Baru, Kecamatan Indralaya Utara, Kabupaten Ogan Ilir, Provinsi Sumatera Selatan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan masyarakat dalam menerapkan teknologi hidroponik vertikultur sebagai alternatif budidaya sayuran di lahan terbatas.Pelaksanaan kegiatan meliputi tahapan survei awal, sosialisasi, pelatihan, demonstrasi, pendampingan, serta evaluasi melalui pre-test dan post-test. Kegiatan diikuti oleh kelompok wanita tani dan masyarakat umum dengan antusiasme tinggi. Hasil evaluasi menunjukkan adanya peningkatan pengetahuan peserta dari rata-rata 50% pada pre-test menjadi 90% pada post-test. Peserta memahami prinsip dasar sistem hidroponik vertikultur, mulai dari pemilihan media tanam, pengaturan nutrisi, hingga perawatan tanaman. Penerapan teknologi hidroponik vertikultur terbukti mampu mengoptimalkan lahan pekarangan menjadi sumber pangan keluarga yang berkelanjutan. Masyarakat mulai mampu memproduksi sayuran segar untuk kebutuhan konsumsi rumah tangga dan memiliki peluang menambah pendapatan dari hasil panen. Kegiatan pengabdian ini berkontribusi terhadap peningkatan kemandirian pangan, perbaikan gizi keluarga, dan pemberdayaan masyarakat pedesaan melalui pemanfaatan teknologi pertanian sederhana dan ramah lingkungan.