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All Journal HAYATI Journal of Biosciences El-Hayah : Jurnal Biologi Folia Medica Indonesiana Jurnal Matematika dan Ilmu Pengetahuan Alam Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati BIOTROPIC The Journal of Tropical Biology Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Riset Biologi dan Aplikasinya Cakrawala Repositori Imwi Narra J Berkala Penelitian Hayati
Alfiah Hayati
Department Of Biology, Faculty Of Science And Technology Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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IMPROVING THE COMPETENCY OF MOLECULAR GENETICS FOR MGMP BIOLOGY TEACHERS IN SENIOR HIGH SCHOOL IN SURABAYA Sugiharto, Sugiharto; Hayati, Alfiah; Amin, M. Hilman F.
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.010-018

Abstract

Molecular Genetics is a compulsory subject in grade 12 of senior high school, covering discussions on Genetic Substances (DNA and RNA), Heredity, Protein Synthesis, Mutations and Biotechnology. Many seniors high school students have difficulty understanding this course, so their report book scores are below the KKM. One of the problems is no practicum to apply this subject because it is constrained by tools, materials, and the skills of the teaching staff at each school. Teachers are a very strategic intermediate target because they will explain directly to their students. The purpose of this community service activity is to improve the competence of teachers who are members of the MGMP Biology in Surabaya, especially in the molecular field. This activity was carried out on Saturday, June 30, 2024, attended by 24 participants coordinated by the Head of the MGMP Biology Surabaya, located at the Molecular Genetics Laboratory, Department of Biology, Faculty of Science and Technology, Airlangga University. During the training, participants were given courses and practicums such as the use of micropipettes, the DNA isolation process, the PCR method and the DNA electrophoresis process. The results of the training showed an increase in teachers' understanding as seen from the comparison of achievement scores between the pre-test (highest score = 50, range of score ​​10 – 20 = 45.83%) and post-test (highest score = 80, range of score ​​10 – 20 = 29.17%); the enthusiasm of participants during the delivery of the courses, practicums and discussions; and the satisfaction index value (above 95%) given by participants. It can be concluded that the Molecular Genetics technique training activities using the PCR and Electrophoresis methods can improve the competence and skills of MGMP Biology Surabaya teachers as an effort to support the learning process in senior high schools. From these results, it is hoped that teachers can be clearer when explaining Molecular Genetics material to their students so that students can understand the material more easily.
Immunomodulatory Activity of Allium sativum, Curcuma mangga, and Acorus calamus Combination Nanoparticle on Mice Leukocytes Profile Muchtaromah, Bayyinatul; Analisa, Nur Izzah; Ahmad, Mujahidin; Fitriasari, Prilya Dewi; Habibi, Soraya; Hayati, Alfiah; Fajriyah, Ely Nuril
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.11879

Abstract

Immunomodulators are substances acting as stimulants or suppressants against the immune system, either to specific or non-specific activity. The non-specific through the production of leukocytes as the first response in fighting against an attacking antigen. Meanwhile, the specific, hrough recognition of antigens by lymphocytes during reattack. This research aimed to determine the immunomodulatory activity of the extracts combination nanoparticles of Allium sativum, Curcuma mangga, and Acorus calamus on the leukocyte profile of mice. This study used 5 treatments and 6 repetitions. The treatment groups were K- (untreated group), P1 (extracts combination nanoparticle dose of 25 mg/kg), P2 (extracts combination nanoparticle dose of 50 mg / kg), P3 (subur kandungan herbal medicine dose of 75 mg / kg), and P4 (Clomiphene citrate dose of 0.9 mg / kgBW). The parameters used included the total number of leukocytes and their differential value. The data that met the parametric assumptions, such as normally distributed and homogeneous were examined using the One Way ANOVA test, and when there was a significant difference, it was processed with the Duncan assessment. While those that did not meet the assumptions were evaluated using a non-parametric analysis. The statistical results showed that administration of extracts combination nanoparticles of A. sativum, C. mangga, and A. calamus at doses of 25 mg/kg and 50 mg/kg were able to suppress the inflammatory reaction by decreasing the total number of leukocytes. However, the differential leukocyte count was able to maintained or modulate immune system, indicating by the percentage of neutrophil, basophil, and eosinophil in the normal range. At a dose of 50 mg/kg, decreased the percentage of lymphocytes, while for monocytes, all dosage ranges were able to increase their number.
Plant-based synthesis of gold and silver nanoparticles using Artocarpus heterophyllus aqueous leaf extract and its anticancer activities Dewi, Firli RP.; Rohmatika, Aulia U.; Jamil, Arniza KM.; Demircan, Turan; Idris, Muhammad F.; Litazkiyyah, Litazkiyyah; Fahmi, Muhammad; Rosyidah, A'liyatur; Hayati, Alfiah; Sugiharto, Sugiharto
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1770

Abstract

Green synthesis of nanoparticles has garnered significant attention for its sustainable and environmentally friendly approach. Despite extensive research on Artocarpus heterophyllus-derived nanoparticles using seeds, fruits, and rind, the therapeutic potential of its leaf extract remains largely unexplored, particularly in MCF-7 breast cancer cells. The aim of this study was to investigate the potential of aqueous leaf extract from A. heterophyllus as a reducing and capping agent to synthesize silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs), as well as to evaluate their anticancer efficacy. The nanoparticles were characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and particle size analysis to confirm the formation. To evaluate anticancer potential, key oncogenes associated with cancer proliferation and survival were analyzed, including c-Myc, cyclin D1, human epidermal growth factor receptor-2 (HER-2), microRNA-622 (miR-622), and cyclooxygenase-2 (COX-2). The present study demonstrated that AgNPs and AuNPs synthesized from A. heterophyllus extract had distinct sizes and shapes, with AgNPs averaging approximately 12.75 nm and exhibiting a spherical morphology, while AuNPs averaged 109.26 nm and had a pentagonal shape. Furthermore, AuNPs had no anticancer activity. In contrast, AgNPs showed potent anticancer effects, with inhibitory concentration (IC50) values of 124.626 and 54.981 µg/mL at 48 and 72 hours, respectively. The AgNPs treatment increased the proportion of cells in G2/M phase, indicating the induction of mitotic catastrophe leading to cell death. AgNPs downregulated the expression of several oncogenes associated with cancer cell proliferation and survival (cyclin D1, COX-2, HER-2, and miR622), but did not significantly reduce c-Myc expression. In conclusion, AgNPs derived from A. heterophyllus leaf extract have significant potential as a novel therapeutic agent in cancer treatment while preserving its biocompatibility, emphasizing the promise of sustainable and cost-effective synthesis of plant-based nanoparticles.
Co-Authors Adelah Putri Milenia Sari Affandi, Moch Agoes Soegianto Agustina, Widayati Amin, M. Hilman F. Analisa, Izza Analisa, Nur Izzah Ansori, Arif NM. Antien Rekyan Seta Aucky Hinting Aunurohim Aurira Thrisna Dwi Aprianti Azizaturrohmah Bayyinatul Muchtaromah Berlian Rustantina Binti Mar`atus Solikha Demircan, Turan Dewi, Firli RP. Dewi, Putri Alicia Duhita, Maharani R. Dwi Winarni Ely Nuril Fajriyah Erlyn Nurul Fauziah Erna Agustina Fajriyah, Ely Nuril Farah Annisa Nurbani Fatma Aulia Nadiyah Fatmi Indah Hati Firdaus, Ana MK. Firli Rahmah Primula Dewi Gebby Agnessya Esa Oktavia Habibi, Soraya Hana Widyana Hanik Faizah I.B. Rai Pidada Idris, Muhammad F. Iman Dary Supriyadi Putra Inesavira Rindaputri Inne Ninda Resita Jamil, Arniza KM. Khaleyla, Firas Khusnita Giarti Ladiya Fitri Ma’rufah Laili Musyarofah Lestari, Lita I.D. Litazkiyyah, Litazkiyyah Manikya Pramudya Maya Safitri Maya Safitri Minarno, Eko B. Muhamad Fadhil Mirza Muhamad Hilman Fu'adil Muhamad Maulana Abdizen MUHAMMAD FAHMI Muhammad Hilman Fu'adil Amin Mujahidin Ahmad Nabilla, Nithasya Nashrulloh, Allam Thoriq Nazil Dwi Rahayuningtyas Ni Wayan Sukma Antari Nina Puspita Ninik Fadhillah Nita Yuliarini Nur Izzah Analisa Nurbani, Farah Annisa Nureka Tiantono Nuris Auliya Nurul Mahmudati Prilya Dewi Fitriasari Princessa Nandita Febrionny Risanda Ulinnuha Rohmatika, Aulia U. Rosmanida Rosyidah, A'liyatur S. Sugiharto Septiani, Adiibtia Setiyowati, Putri Ayu Ika Soesanto Mangkoewidjojo Soraya Habibi Sri Puji Astuti Wahyuningsih Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Sukmaningrum Sugiharto Suhestri Suryaningsih Sukarti Moeljopawiro Trisnadi Widyaleksono Catur Putranto Umi Nur Alfiah Ummi Zubaidah Virid Gibson, Virid Vuanghao Lim Vuanghao, Lim Widi Pangestu Wilujeng Widjiati Widjiati Yuli Winarsih