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PENINGKATAN PENGETAHUAN DAN KETERAMPILAN PEMBUATAN ROLL ON AROMATERAPI DARI MINYAK SEREH SEBAGAI ANTI REPELLENT Yumni, Gharsina Ghaisani; Hidayati, Devi Nisa; K. M., Dewi Andini
Jurnal Abdi Insani Vol 11 No 3 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i3.1839

Abstract

Dengue fever (DF) is a global health problem. The epidemiology of dengue fever in Indonesia varies but continues to increase. Dengue fever itself is caused by the Aedes aegypti (Linn.) mosquito. Indonesia's tropical geographical location provides conditions that support the breeding of disease-carrying mosquitoes. This demonstrates the urgency with which disease-carrying mosquito eradication must be carried out. Currently, mosquito control strategies rely on the use of chemical drugs to control mosquitoes, which often cause side effects. Therefore, many mosquito repellents have been developed based on natural ingredients. Cymbopogon nardus, the plant that yields citronella essential oil, is one of them. The purpose of this PkM project is to increase knowledge regarding the benefits of citronella oil. In addition, it also improves the skills of participants to produce citronella oil as an anti-repellent. This training method is a combination of lectures, demonstrations, and practical actions. Participants will be given counseling regarding the benefits of citronella oil for health. The making of roll-on preparations begins with watching tutorials and live demonstrations by the team which are then followed by the participants. The results of this activity showed an increase in participants' knowledge and skills in making lemongrass aromatherapy roll-on preparations. This can be seen in the PkM product in the form of a roll-on aromatherapy preparation that has a distinctive lemongrass aroma, warm and stable at room temperature. Participants can also plan to make roll-on preparations using other essential oils. The knowledge gained is expected to be developed by participants as entrepreneurial capital. The community service activities were carried out smoothly and according to target. The PkM team succeeded in increasing the knowledge and skills of the participants through PkM activities.
Cytotoxic Activity and Apoptosis by Extract and Ethyl Acetate Fraction of Hibiscus tiliaceus Linn in 4T1 Cell Line Hidayati, Devi Nisa; Safitri, Erika Indah; Surayya, Anis; Alviani, Dwi Lutvi; Putri, Masita Nur Amalia
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 1, FEBRUARY 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Waru leaves are proven to have cytotoxic activity. The activity of waru leaves needs to be developed in a molecular direction to see its apoptotic activity. This study aims to determine the cytotoxic activity of methanol extract (EWL) and ethyl acetate fraction fraction (EAFWL) of Hibiscus tiliaceus Linn as well as the induction of apoptosis in 4T1 cells. The methanol extract of hibiscus leaves was obtained by the maceration method, followed by a fractionation process with n-hexane, ethyl acetate, and water as solvents. Cytotoxic test of EWL was carried out at a concentration of 31.25; 62.5; 125; 250; 500 and 1000 μg/mL while EAFWL was at a concentration of 100; 200; 400; 600 and 100 μg/mL, using MTT assay. Analysis of IC50 values using linear regression. The apoptosis induction test at IC50 and ½ IC50 concentrations using flowcytometry. Data on the percentage of apoptosis were analyzed using One Way Anova statistical analysis. The results were EWL and EAFWL cytotoxic tests against 4T1 had IC50 values of 649 μg/mL and 746 μg/mL. EWL obtained a percentage of total apoptosis at a concentration of 324.5 μg/mL of 48.76%; 649 μg/mL was 67.06% and control cells were 36.16%. EAFWL percentage of total apoptosis at a concentration of 746 μg/mL of 30.8%; 373 μg/mL was 42.13% and control cells were 9.6%. So EWL and EAFWL can induce apoptosis of 4T1 cells.