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GAMBARAN KADAR HEMOGLOBIN PADA SEDIAAN DARAH LENGKAP DI PMI KABUPATEN SLEMAN PROVINSI D.I YOGYAKARTA Ana Nur ‘Aini; Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Prosiding Conference on Research and Community Services Vol 1, No 1 (2019): Prosiding Conference on Research and Community Services)
Publisher : STKIP PGRI Jombang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Darah lengkap merupakan sediaan darah yang mengadung komponen utama berupa eritrosit, trombosit dan faktor pembekuan (V dan VIII). Sediaan darah ini umumnya diberikan pada pasien yang mengalami perdarahan masif, dikarenakan darah lengkap dapat menggantikan kehilangan darah yang terjadi dan memaksimalkan oksigenasi pada organ tubuh pasien. Darah lengkap dapat disimpan hingga 30 hari, pada suhu 2- 6˚C di dalam blood bank. Akan tetapi, masa penyimpanan darah berdampak langsung pada perubahan kualitas darah. Hal ini disebabkan terjadinya stress cells yang memicu lisis pada sel darah. Adapun lisis yang terjadi pada eritrosit akan mempengaruhi kadar hemoglobin dalam darah sedangkan kadar hemoglobin merupakan salah satu indikator kualitas darah. Apabila kadar hemoglobin kurang dari 12,5g/dL darah tidak dapat diberikan pada pasien. Pada penelitian ini dilakukan pengamatan terhadap kadar hemoglobin pada sediaan darah lengkap yang menggunakan antikoagulan CPDA-1. Adapun pengukuran kadar hemoglobin dilakukan sejak masa penyimpanan 0-30 hari menggunakan instrumen hematology analyzer sysmex xs 800i. Hasil pengukuran kadar hemoglobin selama 30 hari penyimpanan  menunjukkan bahwa kadar hemoglobin relatif stabil. Hal ini karena penurunan yang terjadi tidak terlalu signifikan, yaitu  berkisar antara 0,6-2,0%. Oleh karena itu, dapat dinyatakan bahwa  selama 30 hari penyimpanan kadar hemoglobin masih baik digunakan untuk berbagai kondisi medis yang memerlukan transfusi darah lengkap
Bioleaching Ability of Fungi Isolated from an Indonesian Sulfurous River Sediment Serafica Btari Christiyani Kusumaningrum; I Wayan Warmada; Wahyu Wilopo; Endah Retnaningrum
Indonesian Journal of Chemistry Vol 20, No 4 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.29 KB) | DOI: 10.22146/ijc.44810

Abstract

The unique characteristics of sulfurous river sediment located in Ungaran, Indonesia, are a reservoir of novel fungi with manganese bioleaching properties. Fungi are known to produce metabolic organic acids that have a potential for the industrial application of leaching metal from the ores. This application has high advantages, including low cost, low energy, and creates minimal environmental damage. Therefore, this research was performed to analyze the manganese bioleaching activities of two fungal isolates (KA2B2 and KB4B) from Indonesian sulfurous river sediment on pyrolusite and determine their phenotypic characters. These activities were investigated in terms of changes in fungal biomass, soluble manganese concentration, pH reduction, and organic acid production during 16 days of leaching. Soluble manganese concentrations were measured by atomic absorption spectrometry (AAS), whereas organic acid concentrations were analyzed by high-performance liquid chromatography (HPLC). According to bioleaching investigations, KA2B2 strain was more efficient than KB4B1 strain in extracting manganese from 0.02 g/cm3 pyrolusite. It also produced higher levels of organic acids, such as oxalic acid and citric acid, than KB4B1 strain, proving that strain of KA2B2 could be used to extract manganese from pyrolusite. Based on the phenotypic characters, both strains were identified as genus Penicillium.
SINTESIS SENYAWA 4’-HIDROKSI-4-METOKSIKALKON DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI KONTAMINAN PRODUK DARAH Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Vol 7 No 1 (2022): Pharma Xplore : Jurnal Sains dan Ilmu Farmasi
Publisher : Fakultas Farmasi Universitas Buana Perjuangan Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36805/jpx.v7i1.2331

Abstract

Despite of the blood products are crucial for health, it cannot be separated from the risks of bacterial contamination. The bacterial contamination obtained from collecting and processing blood processing. Various species of positive and negative Gram bacteria are known to be bacterial contaminants in blood products such as thrombocyte concentrate, packed red cell, dan whole blood. Therefore, it is essential to find an antibacterial compound that could inhibited the growth of bacterial contaminant in blood products. Chalcone compounds are known to become one of antibacterial since they have an unsaturated-ketone-group which acts as an antibacterial because it can damage the bacterial cell wall. The presence of hydroxyl group has important role for inhibiting bacterial contaminant growth as well. In this research, 4’-hydroxy-4-methoxychalcone was synthesized through the Claisen-Schmidt condensation reaction. The characterization of the chalcone compounds was carried out by mass spectroscopy using gas chromatography-mass spectroscopy (GC-MS) and identification of functional groups using Fourier transform infrared (FTIR). Bacterial contaminants from blood products that were used: Acinetobacter, Escherichia coli, Bacillus sp, and Staphylococcus aureus. In addition, Staphylococcus aureus from the American Type Culture Collection (ATCC) was used as comparing isolates. The antibacterial activity was analyzed by inhibition area method. The results showed that 4’-hydroxy-4-methoxychalcone successfully synthesized with the reaction product in yellow crystal formation with yield of 72,80%, relative purity 100%, and m/z 254. Antibacterial activity of chalcone compounds at various concentrations of 6.25 ppm; 12.5 ppm and 25 ppm against Gram-positive and Gram-negative bacteria are still relatively weak because the percentage of inhibition ability achieved is below 50%.
Evaluasi Kualitas Sediaan Packed Red Cells Hasil Pemrosesan Metode Top and Top Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum; Ikrimah Nafilata; Arum Sari; Aulia Rahman
JI-KES (Jurnal Ilmu Kesehatan) Vol. 6 No. 1 (2022): JI-KES (Jurnal Ilmu Kesehatan)
Publisher : STIKes Hafshawaty Pesantren Zainul Hasan Probolinggo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33006/ji-kes.v6i1.333

Abstract

ABSTRAKSediaan packed red cells dapat diperoleh melalui beberapa metode pemrosesan di antaranya adalah metode top and top, top and bottom serta leukodepleted. Di antara ketiga metode tersebut metode top and top adalah metode yang paling banyak digunakan di berbagai unit pelayanan darah. Pada penelitian ini dilakukan evaluasi kualitas packed red cells metode top and top dengan parameter uji kadar hemoglobin, residual leukosit, hematokrit dan pH darah. Adapun metode penelitian yang digunakan adalah observasional. Hasil pengujian menunjukkan bahwa rerata kadar hemoglobin diperoleh sebesar 49,02±5,51gram/unit, telah melebihi standar minimal hemoglobin yaitu 45gram/ unit. Selaras dengan kadar hemoglobin, kadar hematokrit juga berada pada kisaran ideal 65-75% dengan rerata kadar hematokrit sebesar 70,45±5,36%. Nilai pH darah kantong terukur berada pada rentangan 7,348±0,064, nilai ini sesuai dengan kriteria packed red cells yang baik yaitu memiliki kadar pH>6,71. Adapun parameter yang belum sesuai dengan standar adalah kadar residual leukosit.  Packed red cells yang baik seharusnya memiliki residual leukosit <1,2x109sel/unit sedangkan pada penelitian ini diperoleh rerata residual leukosit sebesar (3,66±0,41)x109sel/unit yang menunjukkan jumlah leukosit tertinggal masih berada pada jumlah yang cukup banyak. Berdasarkan evaluasi yang dilakukan dapat dinyatakan bahwa packed red cells hasil pemrosesan top and top memiliki kualitas baik dari segi kadar hemoglobin, hematokrit dan pH darah namun kurang baik ditinjau dari level residual leukositnya.Kata kunci: hemoglobin, hematokrit, ph darah, packed red cells, residual leukositABSTRACTPacked red cells(PRC) could be obtained by several processing methods such as top and top, top and bottom and leuko-depleted methods. However, the most widely used method in the blood service unit is the top and top method. Therefore, this research was conducted to evaluate the quality of PRC produced from the top and top method with the haemoglobin level, leukocytes residual, hematocrit, and blood pH parameters. The research method used is observational. In addition, this study was performed to know the QC standard accomplishment. The results showed that the average haemoglobin level was 49,02±5,51gram/unit and reached the minimum (45gram/unit).  The average of hematocrit also reached the minimum standard (65-75%) since it obtained  70,45±5,36%. The pH also had included in the sufficient criteria since it had 7,348±0,064, as pH needs to be >6,71. However, the level of the residual leukocyte had not reached the minimum standard yet (1,2x109cells/unit) since the residual leukocytes of PRC in this research were obtained (3,66±0,41)x109cells/unit. It showed that there were a high proportion of leukocytes left. Overall, based on the evaluation, it was concluded that the PRC produced from the top and top method had better quality in the haemoglobin level, hematocrit, and pH but not in the residual leukocyte level. Keywords: hemoglobin, hematocrit, blood pH, packed red cells, residual leukocytes  
Anti-Bacterial Activity of Novel Synthesized Chalcone Against S. epidermidis and S. aureus Isolated from Packed Red Cell Blood Product Aulia Rahman; Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum; Arum Sari; Mohd Nazil Salleh
International Journal of Advanced Health Science and Technology Vol. 2 No. 6 (2022): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v2i6.174

Abstract

Bacterial contamination in blood product was still a problem in Indonesia. Various studies to improve the safety of blood product by inhibiting the bacterial growth in blood product was developed. Chalcone, is one of the natural compounds that has ability to inhibit the bacterial growth, so it can be possible to use that compound for anti-bacterial use in the future. This study aim is to evaluate the anti-bacterial activity of novel synthesis chalcone (E)-1-(4-hydroxyphenyl)-3-phenylprop-2-en-1-one against S. epidermidis and S. aureus isolated from packed red cell blood product. A novel chalcone was synthesized by Claisen-Schmidt Methods while anti-bacterial activity test was done by Agar Diffusion Methods. Chalcone that has been synthesized then characterized by Fourier Transform Infra-Red (FTIR) Methods and Gas Chromatography – Mass Spectrometry (GC-MS). Chalcone was synthesized through a condensation reaction between an aldehyde (benzaldehyde) and ketone (acetophenone) with NaOH as alkaline catalyst. The results from sedimentary analysis using FTIR showed an absorption of the C=C group in the typical medium of chalcone compounds, at the wave number of 1516.96 cm-1. The results of GC-MS characterization resulted a GC chromatogram with one peak with time of retention (tR) 19.68 minutes and relative purity 100%. MS mass spectrum shows that the molecular ion (M+) of this compound was detected at 224 which equivalents to the molecular weight of the chalcone compound. Anti-bacterial test showed that the synthetic chalcone compound with 5% concentration had an inhibitory ability of 56.02% on S. epidermidis and 54.10% on S. aureus, while 2.5% chalcone concentration had an inhibitory ability of 29.17% on S. epidermidis and 50.45% on S. aureus, and 1.25% chalcone concentration had inhibitory ability 0% on S. epidermidis and 37.27% on S. aureus.
Pengaruh Frekuensi Mengkonsumsi Kopi Dengan Kadar Hemoglobin Calon Pendonor Laki-Laki Di PMI Kabupaten Sleman Relita Pebrina; Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum; RIfail
Jurnal Ilmu Kesehatan MAKIA Vol 11 No 2 (2021): Jurnal Ilmu Kesehatan MAKIA
Publisher : LPPM ISTeK ICsada Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.244 KB) | DOI: 10.37413/jmakia.v11i2.173

Abstract

ABSTRAK Donor darah adalah proses pengambilan darah dari seseorang secara sukarela untuk disimpan di bank darah yang kemudian digunakan untuk keperluan transfusi darah. Sebelum pelaksanaan pengambilan darah (aftap), calon donor terlebih dahulu diseleksi untuk mengetahui apakah memenuhi syarat/ kriteria yang ditentukan. Seleksi donor diantaranya adalah pemeriksaan kadar hemoglobin (Hb). Kadar Hb setiap orang berbeda-beda, hal ini dapat dipengaruhi oleh beberapa faktor salah satunya minuman yang populer saat ini adalah kopi. Tujuan penelitian ini adalah mengetahui pengaruh frekuensi minum kopi dengan kadar hemoglobin calon pendonor laki-laki. Jenis penelitian ini bersifat deskriptif korelational dengan pendekatan cross sectional. Sampel penelitian berjumlah 50 responden ditentukan dengan concecutive sampling. Hasil yang didapat dalam penelitian ini yaitu frekunesi mengkonsumsi kopi responden 4 kali dalam seminggu memiliki kadar Hb normal 40,0% lebih banyak dari kadar Hb rendah 2,0% dan kadar Hb tinggi 0,0%, sedangkan responden dengan perilaku >4 kali minum kopi dalam seminggu memiliki kadar Hb normal 30,0% lebih banyak dari kadar Hb rendah 4,0% dan kadar Hb tinggi 0,0%. Responden dengan perilaku 6 kali minum kopi dalam seminggu memiliki kadar Hb normal 20,0% lebih banyak dari kadar Hb rendah 4,0% dan kadar Hb tinggi 0,0%. Kesimpulan penelitian ini adalah terdapat pengaruh frekuensi konsumsi kopi dengan kadar hemoglobin calon pendonor laki-laki dengan nilai signifikasi 0.000.
Antibacterial Activity of 4-hydroxychalcone Against Bacterial Contaminant of Packed Red Cells Serafica Btari Christiyani Kusumaningrum; Wiwit Sepvianti; Relita Pebrina; Aulia Rahman
Indonesian Journal of Biology Education Vol 5, No 2 (2022): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijobe.v5i2.7158

Abstract

The bacterial contamination of blood products is still become a serious problem in consideration, thus it can cause the risk of blood transfusion, namely bacterial sepsis. Packed red cells is blood product and potentially contain bacterial contaminant. The source of bacterial contamination can come from the skin disinfection process in less aseptic during blood collection, donor bacterimia, and  blood processing. In response, currently there have been many antibacterial compounds developments purposely to reduce the risk of bacterial contamination of blood products. This study aimed to observe the activity of 4-hydroxychalconecompounds in contaminant bacteria isolated from packed red cells blood products. Bacterial isolates isolated from the packed red cells were Staphylococcus aureusbased on cell morphology, biochemistry and colony shape. The antibacterial activity of 4-hydroxychalchone compound against Staphylococcus aureus isolates used the diffusion method. The results showed, there were antibacterial activity of 4-hydroxychalchone at a concentration of 2.5% 1.25% and 0.625% against packed red cells contaminant bacteria based upon the clear zone formed. In conclusion, the bacteria isolate was obtained from the PRC was gram positive bacteria, Staphylococcus aureus. The 4-hydroxychalcone had successfully synthesized and has antibacterial activity against S. aureus isolated from PRC, with the biggest inhibition power was 71,36% at 5,0% of chalcone concentration.
Pengaruh Model Pembelajaran Berbasis Proyek terhadap Keterampilan Proses Sains dan Motivasi Belajar Siswa SMA Negeri 1 Candimulyo pada Materi Perubahan Lingkungan Nida Hamidah; Muhammad Radian Nur Alamsyah; Serafica Btari Christiyani Kusumaningrum
Edukhasi: Jurnal Inovasi Pendidikan Vol 1 No 2 (2023): Jurnal Inovasi Pendidikan
Publisher : Edu Berkah Khatulistiwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.621 KB) | DOI: 10.60132/jip.v1i2.37

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh model pembelajaran berbasis proyek terhadap keterampilan proses sains dan motivasi belajar siswa SMA Negeri 1 Candimulyo pada materi perubahan lingkungan. Metode yang digunakan adalah quasi experiment dengan desain pretest-posttest nonequivalent control group. Teknik analisis data menggunakan analisis statistik deskriptif serta uji hipotesis dengan Independent Sample T Test atau Mann Whitney U Test pada taraf signifikansi =0,05. Dari hasil analisis deskriptif data keterampilan proses sains diperoleh nilai rata–rata posttest kelas kontrol sebesar 50,71 dan kelas eksperimen sebesar 78,37. Hasil ini menunjukkan bahwa nilai rata-rata posttest kelas eksperimen lebih tinggi dibanding kelas kontrol. Disamping itu, hasil uji hipotesis dengan Mann Whitney U Test mendapat nilai signifikansi sebesar 0,000 (sig. < 0,05), yang berarti Ha diterima atau terdapat perbedaan keterampilan proses sains yang signifikan antara kelas eksperimen dan kelas kontrol. Kemudian, hasil analisis deskriptif data motivasi belajar siswa memperoleh nilai rata-rata akhir kelas kontrol sebesar 76,19 dan kelas eksperimen sebesar 79,62. Hasil ini menunjukkan bahwa rata-rata akhir kelas eksperimen lebih tinggi dibanding kelas kontrol. Selain itu, uji hipotesis dengan Independent Sample T Test mendapat nilai signifikansi sebesar 0,024 (sig. < 0,05) yang berarti Ha diterima atau terdapat perbedaan motivasi belajar yang signifikan antara kelas eksperimen dan kelas kontrol. Berdasarkan pada hasil penelitian, maka dapat disimpulkan bahwa terdapat pengaruh model pembelajaran berbasis proyek terhadap keterampilan proses sains dan motivasi belajar siswa SMA Negeri 1 Candimulyo pada materi perubahan lingkungan.
Smart and Green Packaging Made from Chitosan-based Biofilm with the Addition of Ginger Oil and Anthocyanins from Butterfly Pea Flower Extract (Clitoria Ternatea L) Fransisca Widhi Mahatmanti; Mohammad Alauhdin; Serafica Btari Christiyani Kusumaningrum
Jurnal Kimia Sains dan Aplikasi Vol 27, No 2 (2024): Volume 27 Issue 2 Year 2024
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.27.2.53-63

Abstract

A chitosan-based biofilm modified with ginger essential oil and butterfly flower extract has been made. This biofilm was tested for its potential as a raw material for smart and environmentally friendly packaging (smart and green packaging). The potential of biofilm as a packaging raw material is known from the characterization results, which include color changes at various pHs, mechanical properties, antioxidant properties, antibacterial properties, and morphological structure. The produced biofilms exhibit sensitivity to alterations in pH levels, manifesting distinct color transitions from pink and purple to green within the pH range of 1 to 12. This phenomenon arises due to adding butterfly flower extract into the biofilm, which contains anthocyanin dyes with a total concentration of 1113.3 mg/L. Adding butterfly flower extract and ginger oil contributes to an augmented biofilm thickness; however, this is accompanied by a reduction in both tensile strength and percent elongation. Concurrently, the addition of butterfly flower extract and ginger oil imparts antioxidant and antibacterial properties to the biofilm. The introduction of additional extracts enhances the antioxidant and antibacterial attributes of the biofilm. In the color and pH response assessment, the biofilm augmented with a 7.5% v/v extract exhibited a color difference value (ΔE) exceeding 5 across all assessed pH values. These findings signify the observable color variations in the biofilm due to pH fluctuations with the unaided eye. According to the outcomes of characterization and analysis, the produced biofilm holds promise as an environmentally friendly packaging solution due to its reliance on natural components and its endowed antioxidant and antibacterial properties, contributing to the prolonged preservation of packaged food items. Moreover, the biofilm demonstrates the capability to gauge the quality of food products based on their pH, which is evident through direct color alterations.
Differences in Fermentation Time Varying Cassava Tapai Substrate, Purple Sweet Potatoes and Banana Kepok Based on Physical Properties, Organoleptic Characteristics and pH Maharani Sopiani; Serafica Btari Christiyani Kusumaningrum; Dian Fajarwati Susilaningrum; Silvia Ardenia; Dewi Fatma Humairoh; Niken Safitri; Ainnatul Khiroh
Indonesian Journal of Biology Education Vol. 7 No. 1 (2024): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tapai is a fermented product that is very popular with the public and has better nutritional value. Using various substrates in producing tapai can be a new alternative in diversifying food processing, one of which is purple sweet potato and Kepok banana, which are a problem in production and occupy the top ranking of agricultural products in Indonesia. Purple sweet potatoes and Kepok bananas have the same essential ingredients as cassava, namely starch. This research was conducted to determine the effect of fermentation time and substrate variations on the physical properties, organoleptic characteristics and acidity levels of cassava tapai, purple sweet potato and Kepok banana. This research was conducted using a completely randomized design experimental research method with a factorial pattern consisting of two factors. The first factor is the substrate factor, and the second is the fermentation time length. The analysis showed that the fermentation time and substrate variations influenced the physical properties, organoleptic characteristics and acidity level of the tapai. Overall, tapai-fermented food was preferred by panelists on the third day of fermentation.