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All Journal Archive of Community Health METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Farmasi Udayana E-Journal Of Animal Science Udayana University Jurnal Veteriner Agrointek Jurnal Peternakan Indonesia Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Bali Anatomy Journal Interdisciplinary Social Studies Public Health and Preventive Medicine Archive
I Nengah Sujaya
Program Studi Ilmu Kesehatan Masyarakat Fakultas Kedokteran Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Veterinary

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Isolasi dan Idetifikasi Bakteri aam Laktat Penghasil Bakteriosin dari Urutan, Sosis Fermentasi Tradisional Bali Ni Made Sri Dwijastuti; I Nengah Sujaya; Ni Nengah Dwi Fatmawati
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p08

Abstract

This study aimed to isolate and identify lactic acid bacteria (LAB) that could produce bacteriocins from urutan. Urutan is a Balinese sausage that is traditionally produced and naturally fermented. Urutans used in this study were obtained from several traders in the Baturiti, Mengwi, and Tegallalang region, Bali. More than 300 LAB colonies were isolated and underwent inhibition test against the growth of the indicator bacteria. Crude bacteriocins from two isolates, namely J2 and J6, showed inhibition zones against the growth of Lactobacillus plantarum E12.1 and Escherichia coli ATCC 8739. The J6 isolate also inhibited the growth of Enterococcus faecalis ATCC 29212. Both J2 and J6 were gram-positive, bacilli shaped, and showed negative catalase test. Based on the results of the 16S rDNA gene amplification, the two isolates were identified as Lactobacillus plantarum. Keywords: Lactic Acid Bacteria, Bacteriocin, Lactobacillus plantarum, Urutan
PEMBINAAN PETANI DI DESA SONGAN, KECAMATAN KINTAMANI-BANGLI MENGENAI PENGGUNAAN PESTISIDA N. M. Utami Dwipayanti; M. Ayu Hitapretiwi Suryadhi; N K. Sutiari; I N. Sujaya; I Agus Gelgel Wirasuta; N T. Suryadhi
Buletin Udayana Mengabdi Vol 11 No 1 (2012): Volume 11 No.1 – April 2012
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

The knowledge of farmers on pesticides and its association on health and the environment still remains an issue. Located in the rural area of Bali, the village of Songan, Kintamani, Bangli with its fertile soil accommodates many farm lands. The counseling and assistance included 20 farmers during their working hours to allow direct observation. The counseling and assistance targeted at issues regarding safe uses of pesticides and its association to health and the environment. The knowledge of participants was evaluated by pre- and post-test. Less than a half (40%) were able to identify an association between pesticide use and health, while almost all do not know the chronic effect of pesticides to health and/or the environment. Recommendations target health care facilitators are expected to continue counseling and assistance to the farmers and others in order to maintain a healthier and more sustainable future.
PEMBINAAN PETANI DI DESA SONGAN, KECAMATAN KINTAMANI-BANGLI MENGENAI PENGGUNAAN PESTISIDA N . M. Utami Dwipayanti; M. Ayu Hita Pretiwi Suryadhi,; N. K. Sutiari; I. N. Sujaya; I M. Agus Gelgel Wirasuta; N. T. Suryadhi
Buletin Udayana Mengabdi Vol 13 No 2 (2014): Vol 13, No. 2 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

The knowledge of farmers on pesticides and its association on health and the environment still remains an issue. Located in the rural area of Bali, the village of Songan, Kintamani, Bangli with its fertile soil accommodates many farm lands. The counseling and assistance included 20 farmers during their working hours to allow direct observation. The counseling and assistance targeted at issues regarding safe uses of pesticides and its association to health and the environment. The knowledge of participants was evaluated by pre- and post-test. Less than a half (40%) were able to identify an association between pesticide use and health, while almost all does not know the chronic effect of pesticides to health and/or the environment. Recommendations target health care facilitators to continue counseling and assistance to the farmers and others in order to maintain a healthier and sustainable future.Keyword: pesticide, health, environment, farmers knowledge
PENGEMBANGAN WIRAUSAHA SANITASI DI WILAYAH KERJA PUSKESMAS KUBU II, KECAMATAN KUBU, KABUPATEN KARANGASEM, BALI I g. H. Purnama; S. G. Purnama; M. A. H. Suryadhi; M. U. Dwipayanti; I. N. Sujaya
Buletin Udayana Mengabdi Vol 15 No 1 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Kebiasaan buang air besar sembarangan masih dilakukan oleh kurang lebih 45% penduduk Indonesia yang umumnya tinggal di pedesaan. Kebiasaan tersebut tidak hanya karena kurangnya kesadaran akan kebutuhan sanitasi, namun juga dikarenakan keterbatasan pilihan teknologi dan sarana sanitasi yang tersedia di tingkat lokal. Puskesmas Kubu II, Kecamatan Kubu telah berusaha menerapkan program Sanitasi Total Berbasis Masyarakat (STBM) untuk menggugah kesadaran masyarakat akan kebutuhan sanitasi dan memfasilitasi untuk terbangunnya aksi kolektif masyarakat untuk menyelesaikan masalah tersebut. Di sisi lain, isu terbuangnya air setelah menyiram closet menjadi isu penting dan berpotensi menjadi hambatan program sanitasi di daerah tersebut. Salah satu upaya yang dilakukan adalah dengan memperkenalkan teknologi Septik Tank Biofilter kepada wirausaha sanitasi di daerah tersebut. Para wirausaha diberikan pembekalan mengenai cara pengaplikasian Septik Tank Biofilter dengan semaksimal mungkin menggunakan media lokal. Selama proses pendampingan kendala yang dihadapi calon pengusaha sanitasi adalah pengetahuan tentang aplikasi teknologi Septik Tank Biofilter dan masih sedikitnya wirausaha yang mau menerapkan teknologi ini sebelum dipasarkan . Semua kendala ini diharapkan dapat diatasi dengan membangun komunikasi antara puskesmas, pemerintah desa, masyarkat pengguna mengenai solusi pendanaan dan dukungan untuk upaya peningkatan kebutuhan.
PEMBINAAN PEDAGANG MAKANAN KAKI LIMA UNTUK MENINGKATKAN HIGIENE DAN SANITASI PENGOLAHAN DAN PENYEDIAAN MAKANAN DI DESA PENATIH, DENPASAR TIMUR I Nengah Sujaya; Ni Made Utami Dwipayanti; Ni Ketut Sutiari; L.P Lila Wulandari; Ni Kadek Tresna Adhi
Buletin Udayana Mengabdi Vol 9 No 1 (2010): Volume 9 No.1 – April 2010
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Abstract Generally street vendor has little knowledge on hygiene and sanitation of food handling. Usually they trade in a night market or on street sides. There are two markets in Penatih Village that operate night market. Both markets located in a strategic location thus they have many visitors every day. In these two markets there are some vendors that need to be counseled and assisted in order to improve their knowledge on hygiene and sanitation of food handling. The counseling and assistance was conducted every week from 25 September to 10 October 2009, with 10 vendors as target participants. The venues were located in Penatih Market and Agung Market, Penatih Village, East Denpasar. The activities were conducted in the form of counseling and assisting during trading hours towards the participants in regards of hygiene and sanitation of food handling. In order to evaluate and measure the knowledge improvement of the participants, pre test and post test were carried out. The result shows that, most of participants have less knowledge on food additives in terms of usage, types and risk of usage. The participants have good knowledge on usage of clean water for utensil and food washing as well as knowledge on personal hygiene. It is recommended to conduct a continuous and regular counseling to street vendors with the involvement of local primary health care (puskesmas).
PENGEMBANGAN STARTER DARI Lactobacillus spp. ISOLAT SUSU KUDA SUMBAWA UNTUK PEMBUATAN SUSU TERFERMENTASI I Gusti Ayu Kusuma Dewi; I Gusti Ngurah Agung Dewantara Putra; I Nengah Sujaya
Jurnal Farmasi Udayana Vol. 2, No. 1, Tahun 2013
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

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Abstract

Susu terfermentasi yang beredar di pasaran menggunakan bakteri probiotik yang bukan berasal dari strain lokal Indonesia. Beberapa strain yang diisolasi dari susu kuda sumbawa menunjukkan potensi untuk dikembangkan menjadi probiotik potensial. Namun, sebagian besar strain-strain belum diuji kemampuannya dalam proses karakterisasi susu untuk membuat susu terfermentasi. Penelitian ini bertujuan untuk menggali potensi Lactobacillus spp. isolat susu kuda Sumbawa sebagai starter susu terfermentasi. Sebanyak 32 isolat Lactobacillus spp. isolat susu kuda Sumbawa yang berpotensi sebagai probiotik diseleksi kemampuannya untuk dikembangkan sebagai starter susu fermentasi yang didasarkan pada produksi massa sel, pH dan karakteristik organoleptik susu terfermentasi. Dari 32 isolat Lactobacillus spp. yang dipergunakan diperoleh 2 isolat potensial yaitu Lactobacillus sp. SMM37 dan Lactobacillus sp. SMM42. Kedua isolat ini dapat memproduksi massa sel yang cepat, menghasilkan susu terfermentasi dengan pH yang baik yaitu Lactobacillus sp. SMM37 sebesar 3,68 dan Lactobacillus sp. SMM42 sebesar 4,19, serta karakteristik organoleptik yang baik yang meliputi rasa, kekentalan (tekstur), ada tidaknya butiran (curd).
Isolasi dan Identifikasi Bakteri Asam Laktat dari Cairan Rumen Sapi Bali sebagai Kandidat Biopreservatif ISOLATION AND IDENTIFICATION OF ACID LACTIC BACTERIA FROM BALI CATTLE’S GASTRIC FLUID AS A POTENTIAL CANDIDATE OF BIOPRESERVATIVE I Wayan Suardana; I Nyoman Suarsana; I Nengah Sujaya; Komang Gede Wiryawan
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to isolate and identify of lactic acid bacteria originated from gastric fluid of bali cattle, and to determine their potential as the candidates of biopreservative. Lactic acid bacteria were isolated by culturing the gastric fluid of bali cattle in de Mann, Rogosa, Sharpe (MRS) medium; screening the bacteria, and identification of bacteria species by Analytical Profile Index (API) 50 CHL Kit. The results showed that, the new species of lactic acid bacteria were isolated and identified as Lactococcus lactis spp lactis 1 (SR21 isolate) and Lactobacillus brevis 1 (SR54 isolate) that have broad spectrum antimicrobial activities. It is clear from this study that a potential lactic acid bacteria producing antimicrobial agent can be isolated from the gastric fluid of bali cattle.
Eksopolisakarida dari Lactobacillus sp. Isolat Susu Kuda Sumbawa dan Potensinya sebagai Prebiotik (EXOPOLYSACCHARIDES FROM LACTOBACILLUS SP. ISOLATED FROM SUMBAWA MARE’S MILK AND ITS POTENTIAL APPLICATION AS PREBIOTICS) I Nengah Sujaya; Ni Putu Desy Aryantini; Ni Wayan Nursini; Cok. Istri Dewiyani Cakrawati; Ni Luh Made Ema Juliasari; Ni Made Utami Dwipayanti; Yan Ramona
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to isolate exopolysaccharides (EPS) producing Lactobacilli isolated fromsumbawa mare’s milk and its potential as prebiotics for modulating the growth of Bifidobacteriumbreve. Nine strains of Lactobacillus sp. were screened for their capabilities to produce EPS usingmodified MRS medium containing sucrose. Prebiotics potential of the EPS was verified by culturingB. breve JCM1273 in TOS medium containing EPS. The results showed that all strains ofLactobacillus sp. produced EPS on MRS sucrose medium and two strains (Lactobacillus SK3.1 andLactobacillus SK4) produced more EPS compared to the other strains tested. Bifidobacteriumbreve JCM1273 showed weak activity while in direct metabolism of EPS produced by Lactobacillussp. SK4 and its growth was enhanced on acid hydrolyzed EPS. Since this phenomenon mighthappened when the EPS exposed by the low pH during gastric passage, hence the EPS might be apotential source to be developed as prebiotics. Nevertheless, further investigation is necessary toevaluate the bifidogenic affects of EPS in Lactobacillus sp. SK4.
Pemberian Gamal Tambahan dalam Ransum Meningkatkan Neraca Nitrogen dan Populasi Mikrob Proteolitik Rumen Sapi Bali (ENHANCEMENT PROVISION OF GLIRICIDIA SEPIUM IN DIET INCREASE NITROGEN BALANCE AND POPULATION OF RUMEN PROTEOLITIK MICROORGANISM OF BALI CATT Ni Nyoman Suryani; I Gede Mahardika; Sentana Putra; Nengah Sujaya
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to study the effect of different forage composition in diet on nitrogen balance andmicrobial population of Bali cattle. Randomized Block Design consisted of four feed treatments with 3block of weight live as replicates were used in this study. Body weight of male bali cattle used rangedbetween 181-265 kg. These four treatments based on dry matter were: A (45% elephant grass + 0% ricestraw + 15% glyricidia + 10% calliandra + 30% concentrate); B (30% elephant grass +10% rice straw + 20%glyricidia + 10% calliandra+ 30% concentrate) ; C (15% elephant grass +20% rice straw + 25% glyricidia+10% calliandra + 30% concentrate) and D (0%elephant grass + 30% rice straw + 30% glyricidia + 10%calliandra+ 30% concentrate) . Variables measured were nitrogen balance and rumen microbial population.The collected data were analyzed by analysis of variance. The result showed that nitrogen intake in cattlefed with diet C was significantly (P<0.05) higher than these in other treatments and nitrogen retention(P<0.05) was significantly higher as compared to those fed with diet A. Amylolytic and cellulolytic bacterialpopulations were not significantly different (P>0.05) among all treatments, but the population of proteolyticbacteria was found the lowest (P<0.05) in cattle fed with diet A. It can be concluded that increasedglyricidia and rice straw in the diet could increased nitrogen intake, nitrogen retention and proteolyticbacterial population.
Analisis Sekuen Probe Gena Shiga Like Toxin-2 dari Isolat Lokal Escherichia coli O157:H7 (PROBE SEQUANCE ANALYSIS OF SHIGA LIKE TOXIN-2 GEN FROM ESCHERICHIA COLI O157:H7 LOCAL ISOLATES) I Wayan Suardana; I Nengah Sujaya; Wayan Tunas Artama
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A Shiga-like toxin producing Escherichia coli O157:H7 was detected in faecal samples of cattle, andhuman as well as in beef. The performance of agent indicated that it has been identified as harmful andoften life-threatening zoonotic agent. It is therefore important to analysed the genetic characteristic ofShiga toxin Escherichia coli (STEC) and to develop a diagnostic probe in order  to optimalized of diagnostictest  for the agent. The  study was started by amplifiying  stx2 gene, purifying of PCR product, sequencingof stx2 gene, analyzing  of phylogenetic tree, and finally  by analyzing   of  diagnostic  probe candidate.Homology study showed that the genetic sequence of the local isolate of  E. coli O157:H7 i.e SM25(1)isolated from cattle feces has  a genetic and fuctional similarity with  the control isolate i.e E. coli O157:H7ATCC 43894 originated from human.  Further study showed that a probe with  foreward primer  sequanceof 5’-AATTTATATGTGGCCGGGTTC-‘3 which were respectively designed as a PFS and PRS 176 bp product.Appeared to be potential candidate of diagnostic probe for the agent.
Co-Authors Cahyawati, Putu Nita Cok. Istri Dewiyani Cakrawati Dwijastuti, Ni Made Sri Exposto, Levi Anatolia S.M. Fukuda Kenji I D.P. Kartika Pratiwi I Dewa Made Sukrama I g. H. Purnama I Gede Mahardika I Gusti Ayu Kusuma Dewi I Gusti Ngurah Agung Dewantara Putra I Komang Gede Wiryawan I Made Agus Gelgel Wirasuta I MADE SUTARGA I Nyoman Suarsana I Wayan Gede Artawan Eka Putra I Wayan Suardana I.D.P.K. Pratiwi Ida Ayu Sri Sinca Maha Uni Kadek Tresna Adhi KOMANG AYU NOCIANITRI Luh Aris Aryandeni Utami Luh Putu Lila Wulandari Made Ayu Hita Pretiwi Suryadhi N T. Suryadhi N. N. Suryani N. T. Suryadhi N.P. Desy Aryantini N.W. Nursini N.W.A. Utami Ni Ketut Sutiari Ni Luh Made Ema Juliasari Ni Luh Putu Suariani Ni Made Utami Dwipayanti Ni Nengah Dwi Fatmawati Ni Nyoman Suryani Ni Putu Desy Aryantini Ni Putu Eka Trisdayanti Ni Putu Suariani Ni Putu Wiastari Ni Putu Widarini Ni Wayan Nursini Putu Rima Sintyadewi S. G. Purnama S. PUTRA S. Putra sang gede purnama Sawitri, Anak Agung Sagung Sentana Putra Tadashu Urashima Wayan Redi Aryanta Wayan Tunas Artama Wulandari, Kristina Yan Ramona Yoshitake Orikasa Yuji Oda