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All Journal Jurnal Akuakultur Rawa Indonesia Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Perikanan dan Kelautan Jurnal Kelautan Tropis JFMR (Journal of Fisheries and Marine Research) BIOEDUSCIENCE Jurnal Penamas Adi Buana Journal of Aquaculture and Fish Health Kontribusia : Research Dissemination for Community Development Grouper: Jurnal Ilmiah Perikanan Jurnal Perikanan Pantura Journal of Fisheries & Marine
Sari, Putri Desi Wulan
Departement Of Aquaculture, Faculty Of Fisheries And Marine, Universitas Airlangga, Jl. Mulyorejo, Mulyorejo, Surabaya 60115, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences Veterinary Other

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Review Artikel : Persebaran Famili Anisakidae yang Menginfeksi Ikan Laut di Indonesia Putri Desi Wulan Sari; Sri Subekti; Yarin Dwi Monica
BIOEDUSCIENCE Vol 5 No 3 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/536358

Abstract

Background: Family of Anisakidae is one of the zoonotic helminth parasitics. The distribution of the family Anisakidae is extensive. It needs to be known because the diversity of parasites can provide information about the distribution area and impact of the parasites of the family Anisakidae in Indonesia. This review article summarises the distribution of parasites from the family Anisakidae that infects marine fish and the factors that influence Indonesia. Methods: This research is qualitative research through literature study as the main object. The data from the literature study results are presented in tables and figures then explained descriptively. Results: The distribution of Anisakidae in Indonesia is found in four genera, namely the genus Anisakis, Pseudoterranova, Terranova and Contracaecum, which are mostly found from the carnivorous marine fish of the family Balistidae, Carangidae, Epinephelidae, Lutjanidae, Priacanthidae, and Scombridae Conclusions: Four genera of endoparasitic worms from the Anisakidae family were reported to infect Indonesian marine fish, including Anisakis, Pseudoterranova, Terranova and Contracaecum. Temperature, salinity, hosts' presence, and anthropogenic factors are biotic and abiotic factors that can affect the distribution of Anisakidae. The information on this literature study results is hoped to be used as the basis for developing research in fish parasitology in particular and increasing public awareness of fish processing in general.
PEMBERDAYAAN SISWA-SISWI SEKOLAH MENENGAH KEJURUAN NEGERI PERIKANAN DALAM PEMBENIHAN IKAN AIR TAWAR Akhmad Taufiq Mukti; Muhamad Amin; Putri Desi Wulan Sari; Veryl Hasan; Gunanti Mahasri; Puguh Yugo Wijanarko; Lia Oktavia Ika Putri; Salman Aldo Alfaresi
Jurnal Penamas Adi Buana Vol 8 No 01 (2024): Jurnal Penamas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/penamas.vol8.no01.a8740

Abstract

The limited knowledge and skills in the hatchery technology application of the freshwater fish to seed production business are obstacles faced by prospective young entrepreneurs in the aquaculture sector. The Community Partnership Program (PKM) was provided as a strategic step to overcome the obstacles faced by the target partner group in this PKM activity in line with the Public Vocational Secondary School's (SMKN) expectations to provide sufficient insight into science and technology for SMKN students as provision for independent entrepreneurship. The aim of this PKM was to transfer the application of freshwater fish (catfish) hatchery technology and the produce fish seeds independently. The activity method was conducted through: a) delivery of material and training related to broodstocks selection and gonad maturation, b) training on the application of freshwater fish hatchery technology (catfish), and c) regular monitoring and assistance to PKM target partner groups by involving related agencies (SMKN). Delivery of material was carried out regularly, both online and face to face (offline). Approximately 40 students of SMKN Ngadirojo, Pacitan Regency who took the flagship program of Aquaculture Agribusiness, attended this activity. This PKM activity was also attended by accompanying teachers. The practical results show an increase in the knowledge and skills of partners in catfish hatchery through the application of semi-artificial catfish broodstocks spawning technology.
Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Rozi; Tyasningsih, Wiwiek; Rahmahani, Jola; Aksono, Eduardus Bimo; Yunus , Muchammad; Al-Arif, Mohammad Anam; Kuncorojati, Suryo; Kusdarwati, Rahayu; Sari, Putri Desi Wulan; Amal, Mohammad Noor Azmai; Salleh, Annas; Khanand, Nadeem; Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.62035

Abstract

Graphical Abstract Highlight Research The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.  
Analisis Kualitas Perairan Berdasarkan Tingkat Kesuburan dan Status Pencemaran Air Pesisir Bomo, Banyuwangi, Indonesia: Analysis of Water Quality Based on Trophic Status and Pollution Status in Bomo Coastal Waters, Banyuwangi, Indonesia Dewi, Nina Nurmalia; Nindarwi, Daruti Dinda; Sari, Putri Desi Wulan; Fauzan, Agung Luthfi
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 3 (2025): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.03.4

Abstract

Perairan pesisir Bomo Banyuwangi merupakan wilayah pesisir yang potensial yang menjadi habitat bagi berbagai organisme akuatik. Adanya pengaruh antropogenik dapat berpotensi mempengaruhi kondisi perairan. Penelitian ini bertujuan untuk mengetahui kondisi lingkungan perairan berdasarkan penentuan tingkat kesuburan dan status pencemaran air pesisir Bomo. Penelitian dilaksanakan di wilayah pesisir Bomo dengan pengambilan sampel di tiga stasiun yang diambil berdasarkan jarak perairan dari daratan. Setiap stasiun diambil sampel sebanyak 3 kali ulangan yang diambil selama 2 bulan pengamatan. Pengambilan sampel meliputi parameter kualitas air yang terdiri dari parameter fisika, kimia, dan biologi yang dianalisis secara insitu maupun exsitu. Data kualitas air dianalisis tingkat kesuburannya menggunakan pendekatan Trophic State Index (TSI) dan Trophic Level Index (TLI). Status pencemaran air dianalisis berdasarkan indeks storet dan indeks pencemaran (pollution index). Berdasarkan hasil penelitian, didapatkan bahwa tingkat kesuburan perairan berada pada kategori eutrofik dengan nilai TSI 74,31-76,58 dan super-eutrofik dengan nilai TLI 6,39-6,84, sedangkan status pencemaran air dipesisir Bomo tergolong memiliki pencemaran yang tinggi dengan nilai indeks storet -37 dan -41 pada bulan Juli dan September dan berdasarkan indeks pencemaran (IP) memiliki nilai 9,10 dan 5,77 untuk masing-masing bulan Juli dan September. Hal ini menandakan bahwa adanya masukan limbah ke perairan yang mempengaruhi kondisi perairan pesisir Bomo.   Bomo Coastal Water Banyuwangi are a potential coastal area that is a habitat for various aquatic organisms. The presence of anthropogenic activity can potentially affect the condition of the waters. This study aims to determine the condition of the aquatic environment based on determining the level of trophic status and pollution status of the Bomo coastal waters. The study was conducted in the coastal area of ​​Bomo by taking samples at three stations taken based on the distance of the waters from the mainland. Each station was sampled 3 times during 2 months of observation. Sampling included water quality parameters consisting of physical, chemical, and biological parameters which were analyzed in situ and ex situ. Water quality data were analyzed for their trophic status using the Trophic State Index (TSI) and Trophic Level Index (TLI) approaches. Pollution status was analyzed based on the storet index and pollution index. Based on the results of the study, it was found that the level of water trophic status was in the eutrophic category with a TSI value of 74.31-76.58 and super-eutrophic with a TLI value of 6.39-6.84, while the status of water pollution on the Bomo coastal waters was classified as having high pollution with a Storet Index value of -37 and -41 in July and September and based on the Pollution Index had a value of 9.10 and 5.77 for July and September respectively. This indicates that there is waste input into the waters that affects the condition of the Bomo coastal waters.
Co-Authors Akbar Maulana Al Ishaqi Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Albet Surya Kembara Amal, Mohammad Noor Azmai Arida Vitasari Daruti Dinda Nindarwi Dewi, Nina Nurmalia Dwi Yuli Pujiastuti Endang Puji Astuti Fahdi Putra Utama Fauzan, Agung Luthfi Ferry Dwi Firmansyah Liananda Gunanti Mahasri Hari Suprapto Indah Hidayati Imani Khanand, Nadeem Kismiyati , Koesnoto Koesnoto Kuncorojati, Suryo Lalu Aldy Kurnia Aji Lia Oktavia Ika Putri Luthfiana Aprilianita Sari Mada Dewantara Mirni Lamid Muhamad Amin Muhammad Ghufron Nafis Putra Laksana Cholil Puguh Yugo Wijanarko Qurrota A'yun Rahayu Kusdarwati Rahmahani, Jola Regita Ardyanti Robi M.A Yahya Rozi Salleh, Annas Salman Aldo Alfaresi Setiawan Koesdarto Siti Hamidah Sri Subekti Suwarno Tjahjaningsih, Wahju Tyasningsih, Wiwiek Veryl Hasan Yarin Dwi Monica Yudin Ardha Pradipta Yunus , Muchammad