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Edukasi Kesehatan Pencegahan Demam Berdarah Dengue pada Siswa SDN 02 Panduman, Desa Jelbuk, Kabupaten Jember Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 3 (2025): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v10i3.6426

Abstract

Dengue hemorrhagic fever (DHF) remains a major public health concern in tropical and subtropical countries, including Indonesia. School-aged children are among the most vulnerable groups due to limited awareness and daily habits that may support mosquito breeding. This community service activity aimed to enhance students’ knowledge of DHF prevention through health education at SDN 02 Panduman, Jelbuk Village, Jember Regency, East Java Province. Educational interventions were delivered interactively using PowerPoint presentations, posters, ovitrap demonstrations, videos, and pocketbooks adapted to the comprehension level of elementary school students. The evaluation was conducted using pre-test and post-test questionnaires consisting of 10 multiple-choice questions on dengue transmission, symptoms, and prevention. The results showed a significant improvement in student knowledge (25%), with average scores increasing from 63% to 88% (p < 0.001, Wilcoxon test). The distribution of illustrated pocketbooks served as an effective reinforcement tool, allowing students to share knowledge with their families and communities. Overall, the intervention successfully raised awareness and fostered preventive behavior among students, demonstrating that school-based health education is an effective strategy for reducing the risk of DHF transmission in endemic rural areas such as Jelbuk Village.
Preliminary study of acute febrile response to crude LPS extract from Salmonella typhimurium in domestic chicken (Gallus gallus domesticus) AKY strain Wijaya, Rizky Surya; Lelono, Asmoro; Oktarianti, Rike
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16139

Abstract

Salmonella is a genus that commonly attacks the health of poultry, especially chickens. One type of poultry that is widely attacked by S. typhimurium is chickens. S. typhimurium infects the host with Lipopolysaccharides (LPS). This study aims to determine the innate immune response of domestic chickens to LPS-based vaccines S. typhimurium as the first step in vaccine development. The sample was in the form of female domestic chickens of the AKY strain which was divided into 3 groups, the control group was injected using 0.1ml of PBS (Phosphate Buffer Saline) treatment group 1 was injected with LPS with an adjuvant with a concentration of 0.5mg/kg as much as 0.1ml. Treatment group 2 was injected with LPS with an adjuvant with a concentration of 1mg/kg of 0.1ml. Injection treatment group using Incomplete Freund Adjuvant (IFA). The results obtained were that the treatment groups had an increase in temperature at the 1st hour to the highest in the 3rd hour with a temperature range of 42.5°C to 42.6°C, contrast to the control group which has a static temperature with a range of 41°C to 41.8°C. The comparison of chicken body temperature among treatment groups using a Liner Mixed Model revealed a significant difference (p < 0.001) between the treatment groups and the control group. These results indicate that subcutaneous LPS administration induces a controlled acute febrile response, which is consistent with involvement of innate inflammatory pathways.
IN-SILICO ANALYSIS OF SYMBIONT BACTERIA DIVERSITY IN THE MIDGUT OF Aedes aegypti USING 16S rDNA MOLECULAR MARKERS DATABASE Syubbanul Wathon; Aufar Finasrullah; Rike Oktarianti; Kartika Senjarini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2841

Abstract

Dengue Hemorrhagic Fever (DHF) is caused by the dengue virus, which is transmitted through Aedes aegypti mosquitoes when they feed on human blood.  To effectively control the DHF vector, it is crucial to accurately characterize the symbiont bacteria associated with Ae. aegypti through an in-silico approach to identify potential targets. This study utilized in- silico analysis based on the 16S rDNA molecular marker to explore the diversity of symbiont bacteria obtained from bioinformatics databases. The analysis and visualization of bacterial diversity were conducted using the Pathosystem Resource Integration Center (PATRIC). The analysis results revealed that bacterial diversity in the midgut of Ae. aegypti, categorized as culturable and non-culturable bacteria, exhibited similar abundance patterns at the family level, albeit with varying detection rates. The most dominant taxa included the phylum Proteobacteria, class Gammaproteobacteria, order Enterobacterales, and family Enterobacteriaceae. Within the culturable bacteria category, the dominant taxa were the genus Salmonella and species Salmonella enterica, whereas the non-culturable bacteria category indicated the prevalence of the genus Escherichia and species Escherichia coli.
IN-SILICO ANALYSIS OF THE INTERACTION BETWEEN D7 PROTEIN FROM THE SALIVARY GLAND OF Ae. albopictus AND Thromboxane A2 FOR DEVELOPING ANTIPLATELET AGENT Syubbanul Wathon; Kartika Senjarini; Rike Oktarianti; Asmoro Lelono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8176

Abstract

The salivary glands of mosquito vector diseases contain various biological components which facilitate blood-feeding into the host's body. These components are mostly protein molecules. Numerous protein molecules in the salivary glands have gained substantial research emphasis to determine their role and function, including those in the salivary glands of Ae. albopictus. D7 protein is the main component in Aedes salivary glands, which aids in inhibiting platelet aggregation by binding to the Thromboxane A2 (TxA2) during the blood-feeding. TxA2 is a eicosanoid molecule that stimulates platelet aggregation. The protein's ability to bind TxA2 shows that this protein has potential as a new antiplatelet agent. The examination of the D7 protein in binding TxA2 was performed through an in-silico approach using the molecular docking method. This research included selecting the 3D model of the D7 protein and the TxA2 ligand, preparing the 3D model of the D7 protein, native ligands, and test ligands, targeted molecular docking method, validating the molecular docking, analysis and visualization of the docking results. The molecular docking validation shows an RMSD value of 1.657 Å. The results of molecular docking show an ΔG value of -5.60 kcal/mol, meaning that the D7 protein can bind to the TxA2 ligand stably and spontaneously. The active site of the D7 protein in binding the TxA2 ligand consists of several amino acid residues, namely THR 190, GLU 268, TYR 178, PHE 154, ILE 175, ARG176, VAL 293, TYR 248, and TYR 178. The ability of D7 protein to bind TxA2 as an inducer of platelet aggregation has demonstrated its potential as a novel antiplatelet agent. These results can pave further development of drug discovery in the medical and pharmaceutical fields.
IN-SILICO ANALYSIS OF THE INTERACTION BETWEEN D7 PROTEIN FROM THE SALIVARY GLAND OF Ae. albopictus AND Thromboxane A2 FOR DEVELOPING ANTIPLATELET AGENT Syubbanul Wathon; Kartika Senjarini; Rike Oktarianti; Asmoro Lelono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8176

Abstract

The salivary glands of mosquito vector diseases contain various biological components which facilitate blood-feeding into the host's body. These components are mostly protein molecules. Numerous protein molecules in the salivary glands have gained substantial research emphasis to determine their role and function, including those in the salivary glands of Ae. albopictus. D7 protein is the main component in Aedes salivary glands, which aids in inhibiting platelet aggregation by binding to the Thromboxane A2 (TxA2) during the blood-feeding. TxA2 is a eicosanoid molecule that stimulates platelet aggregation. The protein's ability to bind TxA2 shows that this protein has potential as a new antiplatelet agent. The examination of the D7 protein in binding TxA2 was performed through an in-silico approach using the molecular docking method. This research included selecting the 3D model of the D7 protein and the TxA2 ligand, preparing the 3D model of the D7 protein, native ligands, and test ligands, targeted molecular docking method, validating the molecular docking, analysis and visualization of the docking results. The molecular docking validation shows an RMSD value of 1.657 Å. The results of molecular docking show an ΔG value of -5.60 kcal/mol, meaning that the D7 protein can bind to the TxA2 ligand stably and spontaneously. The active site of the D7 protein in binding the TxA2 ligand consists of several amino acid residues, namely THR 190, GLU 268, TYR 178, PHE 154, ILE 175, ARG176, VAL 293, TYR 248, and TYR 178. The ability of D7 protein to bind TxA2 as an inducer of platelet aggregation has demonstrated its potential as a novel antiplatelet agent. These results can pave further development of drug discovery in the medical and pharmaceutical fields.
In vitro and in vivo analysis of IgG antibody response against 47 kDa immunogenic protein from salivary gland of Aedes albopictus Oktarianti, Rike; Rizki Amalia, Istiqomah; Firdausi, Shafira; Wathon, Syubbanul; Senjarini, Kartika
Biogenesis: Jurnal Ilmiah Biologi Vol 14 No 1 (2026)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v14i1.55036

Abstract

Dengue Hemorrhagic Fever (DHF) is a disease caused by the dengue virus. The virus is transmitted to humans through the blood-feeding activity of Aedes albopictus. This transmission is facilitated by specific proteins of salivary glands mosquito. Based on the previous studies, there are three immunogenic proteins were detected from salivary glands of Ae. albopictus, i.e 31, 47, and 67 kDa. The 47 kDa protein is one of the proteins suspected to be a serpin protein. These proteins are capable of inducing an immune response in the host. This study aims to assess the capacity of the 47 kDa immunogenic protein derived from the salivary glands of Ae. albopictus to elicit host immune responses. This is achieved through IgG analysis conducted both in vitro and in vivo analysis. In vitro IgG antibody response analysis was performed on human serum samples from healthy people, DHF patients and neonates collected from Jember. In vivo IgG antibody response analysis was performed on mice (Mus musculus) that had been injected with 47 kDa protein. IgG level measurement using the ELISA indirect method. According to in vitro examination of IgG antibody response in human samples against 47 kDa salivary protein, the highest IgG levels were detected in healthy samples followed by dengue patients and neonates. This suggests that the 47 kDa protein from Ae. albopictus salivary glands is recognized by human serum and developed into a biomarker for mosquito bites. In vivo examination of the IgG antibody response in mice (M. musculus) injected by 47 kDa protein revealed that the IgG antibody might be increased by repeated exposure to 47 kDa protein. The highest  IgG level was detected in the 6th week after repeated exposure. Repeated exposure of the 47 kDa salivary protein from Ae. albopictus have been demonstrated to elicit a humoral immune response in mice.
Peningkatan Pengetahuan dan Kepedulian Masyarakat melalui Kegiatan Edukasi Kesehatan untuk Pencegahan Penyakit Malaria Syubbanul Wathon; Kartika Senjarini; Rike Oktarianti
Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2026): Edisi November 2026
Publisher : FKIP Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36709/amalilmiah.v7i3.559

Abstract

Malaria merupakan penyakit infeksi Plasmodium sp. yang ditularkan melalui vektor nyamuk Anopheles sp. kepada manusia. Beberapa daerah di Indonesia masih ditemukan kasus penularan malaria bahkan menjadi lokasi endemik malaria. Desa Bangsring, Kecamatan Wongsorejo, Kabupaten Banyuwangi pernah tercatat kejadian luar biasa malaria. Kondisi lingkungan pada daerah tersebut mendukung sebagai tempat perindukan Anopheles sp. sehingga meningkatkan potensi munculnya kasus malaria. Tujuan kegiatan ini untuk memberikan edukasi kepada masyarakat di Desa Bangsring mengenai upaya pencegahan penyakit malaria serta memberikan pemahaman mengenai usaha-usaha pemberantasan jentik dan nyamuk Anopheles sp. untuk meminimalkan resiko penularan malaria. Metode pada kegiatan ini meliputi tahapan persiapan kemudian pelaksanaan kegiatan meliputi presentasi materi dan diskusi interaktif lalu diakhiri dengan evaluasi melalui instrument pre-test dan post-test untuk mengetahui efektivitas pelaksanaan kegiatan. Hasil kegiatan menunjukkan bahwa pemahaman awal masyarakat terkait penyakit malaria sebesar 65% kemudian mengalami peningkatan menjadi 88% di akhir kegiatan. Hal ini menunjukkan adanya peningkatan pengetahuan dan kepedulian kepada masyarakat mengenai bahaya penyakit malaria dan penangulangannya. Dengan demikian, pelaksanaan edukasi pencegahan penyakit malaria mendorong partisipasi masyarakat untuk hidup sehat serta peduli dalam menjaga kebersihan lingkungan sekitar guna memutus penularan malaria.
Co-Authors Agustin, Dita Paramytha Ahmad Tosin Aida Mursyidah Ainiyah, Durotun Ali Maziun, Iffa Amania, Novita Aminuyati Anam, Khairul Anik Swandari Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Arofa, Resmining Mega Aufar Finasrullah Azkiyah, Siti Fat'hiyatul Berlian Permata Dewi Erlambang Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Dita Paramytha Agustin Dwi Setyati Endah Puspitasari Eva Tyas Utami Febriyantiningsih, Dwi Esti Firdausi, Shafira Fitria Mutiah Fitria Muti’ah Hasanah, Fitrianti Milhatul Hidayat Teguh Wiyono Iflaha, Adriana Ihsanul Azmi Indriani, Fenny Intan Fitri Indrasari Kartika Senjarini Kartika Senjarini Khasanah, Rochmatul Nuryu Kirana Eka Rezki Labes, Antje Labes, Antje Laili, Alvina Nur Lailly Nur Uswatul Hasanah Lelono, Asmoro Lutfia Azizah Mahriani Mahriani Mamik Pristiwindari MAsruroh, Binti Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Nailatur Rifdah Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nihayah, Husnatun Novita Amania Nur Aisyah Septiana Nuril Azizah Oktavia, Melinda Dwi Putri Purwatiningsih Purwatiningsih Putra, Iguh Widigda Rahmawati, Itsna Ratis Nour Sholichah Rehmann, Holger Renam Putra Arifianto Rendy Setiawan Riana Agatha Listiani Rizki Amalia, Istiqomah Saraswati, Dyah Rahmadhani Silvya Fitri Nur Azizah Siti Agustina Wulandari Sri Mumpuni Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Syubbanul Wathon Syubbanul Waton Syubhanul Wathon Tri Yudani MR Utami, Diah Ayu Wahyu Tri Agustin Wathon, Syubanul Wathon, Syubbanul Widya Cahyadi Widyadana, Kaysha Rahmadita Wijaya, Rizky Surya Wulandari, Ayu Dwi Yasir Mubarok Yenny Febriana Ramadhan Abdi Zahniar, Zahniar