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All Journal ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems Jurnal Ilmu Dasar AL KAUNIYAH BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi UNEJ e-Proceeding Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Bioma : Jurnal Biologi dan Pembelajaran Biologi J-Dinamika: Jurnal Pengabdian Kepada Masyarakat SULUH: Jurnal Abdimas Jurnal Riset Biologi dan Aplikasinya Life Science and Biotechnology Bioeksperimen Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Rike Oktarianti
Biology Department, Faculty Of Mathematic And Natural Sciences, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Veterinary Other

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IN-SILICO ANALYSIS OF THE INTERACTION BETWEEN D7 PROTEIN FROM THE SALIVARY GLAND OF Ae. albopictus AND Thromboxane A2 FOR DEVELOPING ANTIPLATELET AGENT Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike; Lelono, Asmoro
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8176

Abstract

The salivary glands of mosquito vector diseases contain various biological components which facilitate blood-feeding into the host's body. These components are mostly protein molecules. Numerous protein molecules in the salivary glands have gained substantial research emphasis to determine their role and function, including those in the salivary glands of Ae. albopictus. D7 protein is the main component in Aedes salivary glands, which aids in inhibiting platelet aggregation by binding to the Thromboxane A2 (TxA2) during the blood-feeding. TxA2 is a eicosanoid molecule that stimulates platelet aggregation. The protein's ability to bind TxA2 shows that this protein has potential as a new antiplatelet agent. The examination of the D7 protein in binding TxA2 was performed through an in-silico approach using the molecular docking method. This research included selecting the 3D model of the D7 protein and the TxA2 ligand, preparing the 3D model of the D7 protein, native ligands, and test ligands, targeted molecular docking method, validating the molecular docking, analysis and visualization of the docking results. The molecular docking validation shows an RMSD value of 1.657 Å. The results of molecular docking show an ΔG value of -5.60 kcal/mol, meaning that the D7 protein can bind to the TxA2 ligand stably and spontaneously. The active site of the D7 protein in binding the TxA2 ligand consists of several amino acid residues, namely THR 190, GLU 268, TYR 178, PHE 154, ILE 175, ARG176, VAL 293, TYR 248, and TYR 178. The ability of D7 protein to bind TxA2 as an inducer of platelet aggregation has demonstrated its potential as a novel antiplatelet agent. These results can pave further development of drug discovery in the medical and pharmaceutical fields.
PURIFIKASI FRAKSI PROTEIN IMUNOGENIK 47 kDa DARI KELENJAR SALIVA Aedes albopictus SEBAGAI TARGET PENGEMBANGAN VAKSIN DENGUE BERBASIS VEKTOR Wathon, Syubbanul; Rahmawati, Itsna; Oktarianti, Rike; Lelono, Asmoro; Senjarini, Kartika
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.810

Abstract

Kapasitas vektorial Ae. albopictus telah diketahui sebagai vektor potensial virus dengue yang mengakibatkan DBD. Transmisi virus dengue terjadi ketika Ae. albopictus melakukan blood feeding ke manusia yang difasilitasi oleh aktivitas biologis protein pada kelenjar saliva vektor. Penelitian kami sebelumnya menunjukkan fraksi protein 47 kDa dari kelenjar saliva Ae. albopictus bersifat imunogenik. Protein 47 kDa merupakan serpins family yang diduga sebagai antikoagulan dan berperan sebagai protease inhibitor sehingga mempermudah transmisi virus dengue. Peran protein tersebut mengindikasikan adanya potensi dalam pengembangan vaksin penghambat transmisi patogen melalui vaksinasi melawan protein tersebut sehingga dapat menghambat transmisi dengue. Studi potensi protein tersebut memerlukan ekstrak murni, maka purifikasi protein target merupakan langkah yang sangat penting. Penelitian ini dimulai dengan landing collection dan rearing Ae. albopictus, isolasi dan ekstraksi protein kelenjar saliva, isolasi fraksi protein 47 kDa melalui SDS-PAGE, purifikasi protein, analisis dot blot dan western blot. Analisis SDS-PAGE menunjukkan pita tunggal protein target 47 kDa dari kelenjar saliva Ae. albopictus yang berhasil dipurifikasi menggunakan Electroeluter. Analisis dot blot menunjukkan protein 47 kDa terdeteksi bersifat imunogenik dan dikonfirmasi melalui analisis western blot bahwa protein target hasil purifikasi memiliki berat molekul 47 kDa.
PROFIL BIONOMIK VEKTOR POTENSIAL DENGUE DI KECAMATAN SUMBERSARI, KABUPATEN JEMBER Wathon, Syubbanul; Ali Maziun, Iffa; Oktarianti, Rike; Senjarini, Kartika
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.9215

Abstract

Kabupaten Jember menempati urutan ketiga tertinggi kasus Demam Berdarah Dengue (DBD) di Jawa Timur, dengan angka tertinggi di Kecamatan Sumbersari. Beberapa upaya untuk memberantas kasus DBD telah dilakukan, misalnya pemberantasan sarang nyamuk (3M plus), fogging, penggunaan larvasida, “Gerakan Jumantik” namun masih ada kasus DBD khususnya di daerah endemik. Upaya penanggulangan DBD memerlukan studi bionomik untuk mencapai strategi yang efektif dalam program pengendalian vektor. Tujuan penelitian ini adalah untuk mengetahui bionomik vektor potensial DBD di wilayah endemik DBD Kecamatan Sumbersari, Jember, yang meliputi: identifikasi morfologi, proporsi jenis dan perilaku istirahat vektor nyamuk, serta karakteristik habitat dan kepadatan larva vektor nyamuk. Penelitian ini dilakukan dengan pendekatan metode purposive sampling pada beberapa lokasi di Kecamatan Sumbersari pada bulan September 2020. Koleksi sampel larva Aedes dilakukan dengan single larva methods sedangkan nyamuk dikoleksi dengan metode resting collection di dalam maupun luar rumah. Hasil penelitian menunjukkan bahwa proporsi Ae. aegypti (91%) lebih tinggi dibandingkan dengan Ae. albopictus (9%). Perilaku istirahat Ae. aegypti menunjukkan karakter endofilik, sedangkan Ae. albopictus lebih bersifat eksofilik. Puncak aktivitas istirahat Aedes spp. terjadi pada pukul 09.00–10.00 WIB. Karakteristik tempat perindukan Aedes spp. ditemukan di kontainer air berupa bak mandi (62%, dibandingkan jenis ember dan pot), berbahan semen (46%, dibandingkan bahan plastik dan keramik), berwarna gelap (77%, dibandingkan dengan berwarna terang) dan terletak di dalam ruangan (85%, dibandingkan dengan di luar ruangan). Kepadatan larva berdasarkan indek House Indicator di Kecamatan Sumbesari menunjukkan angka sebesar 30%. Berdasarkan tabel resiko penularan DBD, maka resiko penularan DBD pada Kecamatan Sumbersari termasuk dalam kategori tinggi.
Edukasi Kesehatan Pencegahan Demam Berdarah Dengue pada Siswa SDN 02 Panduman, Desa Jelbuk, Kabupaten Jember Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 3 (2025): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v10i3.6426

Abstract

Dengue hemorrhagic fever (DHF) remains a major public health concern in tropical and subtropical countries, including Indonesia. School-aged children are among the most vulnerable groups due to limited awareness and daily habits that may support mosquito breeding. This community service activity aimed to enhance students’ knowledge of DHF prevention through health education at SDN 02 Panduman, Jelbuk Village, Jember Regency, East Java Province. Educational interventions were delivered interactively using PowerPoint presentations, posters, ovitrap demonstrations, videos, and pocketbooks adapted to the comprehension level of elementary school students. The evaluation was conducted using pre-test and post-test questionnaires consisting of 10 multiple-choice questions on dengue transmission, symptoms, and prevention. The results showed a significant improvement in student knowledge (25%), with average scores increasing from 63% to 88% (p < 0.001, Wilcoxon test). The distribution of illustrated pocketbooks served as an effective reinforcement tool, allowing students to share knowledge with their families and communities. Overall, the intervention successfully raised awareness and fostered preventive behavior among students, demonstrating that school-based health education is an effective strategy for reducing the risk of DHF transmission in endemic rural areas such as Jelbuk Village.
Preliminary study of acute febrile response to crude LPS extract from Salmonella typhimurium in domestic chicken (Gallus gallus domesticus) AKY strain Wijaya, Rizky Surya; Lelono, Asmoro; Oktarianti, Rike
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16139

Abstract

Salmonella is a genus that commonly attacks the health of poultry, especially chickens. One type of poultry that is widely attacked by S. typhimurium is chickens. S. typhimurium infects the host with Lipopolysaccharides (LPS). This study aims to determine the innate immune response of domestic chickens to LPS-based vaccines S. typhimurium as the first step in vaccine development. The sample was in the form of female domestic chickens of the AKY strain which was divided into 3 groups, the control group was injected using 0.1ml of PBS (Phosphate Buffer Saline) treatment group 1 was injected with LPS with an adjuvant with a concentration of 0.5mg/kg as much as 0.1ml. Treatment group 2 was injected with LPS with an adjuvant with a concentration of 1mg/kg of 0.1ml. Injection treatment group using Incomplete Freund Adjuvant (IFA). The results obtained were that the treatment groups had an increase in temperature at the 1st hour to the highest in the 3rd hour with a temperature range of 42.5°C to 42.6°C, contrast to the control group which has a static temperature with a range of 41°C to 41.8°C. The comparison of chicken body temperature among treatment groups using a Liner Mixed Model revealed a significant difference (p < 0.001) between the treatment groups and the control group. These results indicate that subcutaneous LPS administration induces a controlled acute febrile response, which is consistent with involvement of innate inflammatory pathways.
Co-Authors Agustin, Dita Paramytha Ahmad Tosin Aida Mursyidah Ainiyah, Durotun Ali Maziun, Iffa Amania, Novita Aminuyati Anam, Khairul Anik Swandari Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Arofa, Resmining Mega Aufar Finasrullah Azkiyah, Siti Fat'hiyatul Berlian Permata Dewi Erlambang Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Dita Paramytha Agustin Dwi Setyati Endah Puspitasari Eva Tyas Utami Febriyantiningsih, Dwi Esti Finasrullah, Aufar Fitria Mutiah Fitria Muti’ah Hasanah, Fitrianti Milhatul Hidayat Teguh Wiyono Iflaha, Adriana Ihsanul Azmi Indriani, Fenny Intan Fitri Indrasari Kartika Senjarini Khasanah, Rochmatul Nuryu Kirana Eka Rezki Labes, Antje Labes, Antje Laili, Alvina Nur Lailly Nur Uswatul Hasanah Lelono, Asmoro Lutfia Azizah Mahriani Mahriani Mamik Pristiwindari MAsruroh, Binti Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Nailatur Rifdah Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nihayah, Husnatun Novita Amania Nur Aisyah Septiana Nuril Azizah Oktavia, Melinda Dwi Putri Purwatiningsih Purwatiningsih Putra, Iguh Widigda Rahmawati, Itsna Ratis Nour Sholichah Rehmann, Holger Renam Putra Arifianto Rendy Setiawan Riana Agatha Listiani Saraswati, Dyah Rahmadhani Silvya Fitri Nur Azizah Siti Agustina Wulandari Sri Mumpuni Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Syubbanul Waton Syubhanul Wathon Tri Yudani MR Utami, Diah Ayu Wahyu Tri Agustin Wathon, Syubanul Wathon, Syubbanul Widya Cahyadi Widyadana, Kaysha Rahmadita Wijaya, Rizky Surya Wulandari, Ayu Dwi Yasir Mubarok Yenny Febriana Ramadhan Abdi Zahniar, Zahniar