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EFEK EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP JUMLAH PROTEIN GLUT4 PADA TIKUS PUTIH HIPERGLIKEMIK Kirtishanti, Aguslina; Budiono, Ryanto; Ratih, .; Isfandiari, Fitria
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 4, No 2 (2008)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Difficulties in finding anti-diabetics which are effective, tolerable by the patients, safe, cause people to try to find other alternative, such as taking traditional medicine like “mengkudu” [Morinda citrifolia L.]. Mengkudu fruit had been proven to be able to increase the number of protein GLUT4 at type 2 diabetic male white rat’s sceletal muscle. Fruit of mengkudu has similar chemical ingredient with its leaves. So far, however, mengkudu leaves had not been studied to increase the number of protein GLUT4 at sceletal muscle. This study use male white rats Wistar strain. Total number of white rats involved in this study were 40 rats which treated : 10 rats as negative control group [no treatment], 10 rats as positive control group and they are treated with exogen insulin 0,35 U per body weight three times daily for 10 days. The last 20 rats is divided into 2 groups with 10 rat for each group as first trial group and second trial group. The trial group are treated with exogen insulin and first trial group were given water extract of mengkudu leaves 7,5 g per body weight and second trial group were given alcohol extract of mengkudu leaves 1,8 g per body weight twice daily which were administered starting from the seventh to tenth day of exogen insulin administration. After ten days of administration, blood glucose level of all rats was measured and all rats were killed afterwards. The next step is sceletal muscle tissue processing to be painted immunohistochemistry to GLUT4. Conclusion of the result is water extract and alcohol extract of mengkudu leaves  could not increase the number of protein GLUT4 at type 2 diabetic male white rats. ABSTRAK Kesulitan mendapatkan obat antidiabetes yang efektif dan aman menyebabkan pasien mencoba obat alternatif, misalnya dengan menggunakan obat tradisional “mengkudu” (Morinda citrifolia L.). Buah mengkudu telah terbukti dapat meningkatkan jumlah protein Glut4 pada otot rangka tikus putih jantan diabetik. Buah mengkudu memiliki kandungan kimia yang hampir sama dengan daunnya. Oleh sebab itu dalam penelitian ini dilakukan penelitian untuk mengetahui apakah daun mengkudu juga dapat meningkatkan jumlah protein Glut4 seperti buahnya. Dalam penelitian ini digunakan 40 tikus putih jantan strain Wistar yang dibagi menjadi 4 kelompok, yaitu kelompok kontrol negatif, kelompok kontrol positif yang diberi insulin eksogen 0,35 U per berat badan tiga kali sehari selama 10 hari, kelompok perlakuan pertama yang diberi ekstrak air daun mengkudu 7,5 g per kg berat badan, dan kelompok perlakuan kedua yang diberi ekstrak alkohol daun mengkudu 1,8 g per kg berat badan dua kali sehari mulai hari ke-7 sampai hari ke-10. Kadar gula darah diukur setelah hari ke-10. Jaringan otot rangka diambil dan dilakukan pewarnaan untuk melihat protein Glut4. Dari hasil penelitian yang diperoleh disimpulkan bahwa ekstrak air dan alkohol daun mengkudu tidak dapat meningkatkan jumlah protein Glut4 pada tikus putih jantan diabetik tipe 2.
EFEK EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP JUMLAH PROTEIN GLUT4 PADA TIKUS PUTIH HIPERGLIKEMIK Kirtishanti, Aguslina; Budiono, Ryanto; Ratih, .; Isfandiari, Fitria
Jurnal Farmasi Indonesia Vol 4, No 2 (2008)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v4i2.10

Abstract

Difficulties in finding anti-diabetics which are effective, tolerable by the patients, safe, cause people to try to find other alternative, such as taking traditional medicine like â??mengkuduâ? [Morinda citrifolia L.]. Mengkudu fruit had been proven to be able to increase the number of protein GLUT4 at type 2 diabetic male white ratâ??s sceletal muscle. Fruit of mengkudu has similar chemical ingredient with its leaves. So far, however, mengkudu leaves had not been studied to increase the number of protein GLUT4 at sceletal muscle. This study use male white rats Wistar strain. Total number of white rats involved in this study were 40 rats which treated : 10 rats as negative control group [no treatment], 10 rats as positive control group and they are treated with exogen insulin 0,35 U per body weight three times daily for 10 days. The last 20 rats is divided into 2 groups with 10 rat for each group as first trial group and second trial group. The trial group are treated with exogen insulin and first trial group were given water extract of mengkudu leaves 7,5 g per body weight and second trial group were given alcohol extract of mengkudu leaves 1,8 g per body weight twice daily which were administered starting from the seventh to tenth day of exogen insulin administration. After ten days of administration, blood glucose level of all rats was measured and all rats were killed afterwards. The next step is sceletal muscle tissue processing to be painted immunohistochemistry to GLUT4. Conclusion of the result is water extract and alcohol extract of mengkudu leaves  could not increase the number of protein GLUT4 at type 2 diabetic male white rats. ABSTRAK Kesulitan mendapatkan obat antidiabetes yang efektif dan aman menyebabkan pasien mencoba obat alternatif, misalnya dengan menggunakan obat tradisional â??mengkuduâ? (Morinda citrifolia L.). Buah mengkudu telah terbukti dapat meningkatkan jumlah protein Glut4 pada otot rangka tikus putih jantan diabetik. Buah mengkudu memiliki kandungan kimia yang hampir sama dengan daunnya. Oleh sebab itu dalam penelitian ini dilakukan penelitian untuk mengetahui apakah daun mengkudu juga dapat meningkatkan jumlah protein Glut4 seperti buahnya. Dalam penelitian ini digunakan 40 tikus putih jantan strain Wistar yang dibagi menjadi 4 kelompok, yaitu kelompok kontrol negatif, kelompok kontrol positif yang diberi insulin eksogen 0,35 U per berat badan tiga kali sehari selama 10 hari, kelompok perlakuan pertama yang diberi ekstrak air daun mengkudu 7,5 g per kg berat badan, dan kelompok perlakuan kedua yang diberi ekstrak alkohol daun mengkudu 1,8 g per kg berat badan dua kali sehari mulai hari ke-7 sampai hari ke-10. Kadar gula darah diukur setelah hari ke-10. Jaringan otot rangka diambil dan dilakukan pewarnaan untuk melihat protein Glut4. Dari hasil penelitian yang diperoleh disimpulkan bahwa ekstrak air dan alkohol daun mengkudu tidak dapat meningkatkan jumlah protein Glut4 pada tikus putih jantan diabetik tipe 2.
PKM Pemanfaatan Potensi Pangan Lokal Ubi Jalar Cilembu melalui Penepungan dan Pengolahan Mie Kering di Kabupaten Mojokerto Trisnawati, Chatarina Yayuk; Kirtishanti, Aguslina; Irawati, Sylvi; Dewi, Ardhia Deasy rosita
GUYUB: Journal of Community Engagement Vol 4, No 3 (2023)
Publisher : Universitas Nurul Jadid

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33650/guyub.v4i3.7200

Abstract

Duyung Village, located in Trawas District, Mojokerto Regency, has local food potential for Cilembu sweet potatoes. The problem that arises is that the use of local food is not yet optimal. Processing into Cilembu sweet potato flour and dry noodles is a step to optimize its utilization. This community service activity aims to develop the local food potential of Cilembu sweet potatoes in order to achieve food security in Duyung Village. Partners for this community service activity are farmer group partners and PKK groups. Training activities on making Cilembu sweet potato flour and dry noodles made from Cilembu sweet potato flour have been able to increase partners' knowledge and skills regarding the processing of Cilembu sweet potato flour and its application in dry noodle products. Mitra is also enthusiastic about making Cilembu sweet potato flour and dry noodles which will later become a business unit. The establishment of the Cilembu sweet potato flour and dry noodle business unit is expected to increase food security in Duyung Village through utilizing local food potential
Pengaruh Jenis dan Dosis Vaksin Covid-19 Terhadap Kejadian Ikutan Pasca Imunisasi di Puskesmas Poncokusumo Kabupaten Malang Yuliastuti, Reny; Purnamayanti, Anita; Kirtishanti, Aguslina
Journal of Islamic Pharmacy Vol 8, No 2 (2023): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v8i2.23684

Abstract

Terdapat beberapa jenis vaksin Covid-19 yang telah diberikan untuk mencegah infeksi SARS-CoV-2, namun perlu dilengkapi dengan data keamanan pada subjek Indonesia. Tujuan penelitian ini untuk menganalisis Kejadian Ikutan Pasca Imunisasi (KIPI) vaksin COVID-19 pada subjek yang diimunisasi di Puskesmas Poncokusumo Kabupaten Malang. Jenis vaksin yang digunakan yaitu CoronaVac, Pfizer, Astra Zeneca, dan Moderna. Data diperoleh dari aplikasi P-care, kuisioner, dan wawancara. Desain penelitian adalah potong lintang. Subjek penelitian usia > 15 tahun, yang telah menerima vaksin hingga dosis ketiga (booster). Kriteria eksklusi penelitian adalah wanita hamil dan menyusui. Total sampel penelitian ini adalah 315 orang dengan karakteristik responden adalah laki-laki (34,96%) dan perempuan (65,08%). CoronaVac paling banyak digunakan pada pemberian dosis pertama dan kedua, sedangkan Astra Zeneca paling banyak digunakan sebagai booster. KIPI yang terbanyak terjadi adalah pada dosis ketiga berupa nyeri tempat injeksi dan demam. Hasil uji regresi linear berganda yaitu (a) uji T parsial menunjukkan bahwa terdapat perbedaan bermakna (p=0,004) angka kejadian KIPI pada dosis vaksin pertama, kedua, dan ketiga (booster), namun jenis vaksin tidak berdampak pada angka kejadian KIPI (p=0,492); serta (b) uji F simultan menunjukkan bahwa terdapat pengaruh pemberian jenis dan dosis vaksin secara simultan terhadap angka kejadian KIPI (p=0.013). Dapat disimpulkan bahwa jenis dan dosis vaksin secara bersama-sama mempengaruhi angka kejadian KIPI (p=0.013).
Pencegahan dan Penanganan Skabies di Desa Pulopancikan, Gresik Novansca, Tiara Prima Ninting; Sukmantoro, Agung Anjar; Nastiti, Widhi; Wiana, Viera Permatasari; Rahman, Diana; Buono, Iqhro’ Samudro Thirto; Buono, Khresno Bhirowo Ghusti; Hidayati, Siti Hafida Nur; Wijayaningrum, Wijayaningrum; Kirtishanti, Aguslina; Setiawan, Eko; Mulyono Putri Wibowo, Ika; Susilo, Astrid Pratidina
Warta LPM WARTA LPM, Vol. 27, No. 2, Juli 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/warta.v27i2.4743

Abstract

Scabies is a contagious skin disease with a predisposition to people who live in densely populated areas. Pulopancikan Village has the highest prevalence of scabies among other villages in Gresik, East Java. From the results of a survey of visits to the homes of Pulopancikan Village residents, it was concluded that the high level of scabies transmission in the village occurred due to a lack of public knowledge about the disease, especially in terms of prevention and treatment. The aim of this program is to intervene in the form of community education, creating educational feedback sheets, and distributing checklists for preventing and treating scabies at home. This intervention is aimed at mothers or families who have children with scabies health problems. Knowledge tests were carried out before and after the intervention to evaluate the project, which showed a significant difference in increasing the knowledge of the Pulopancikan Village community (p<0.05). However, in this program, the long-term impact of increasing this knowledge cannot yet be known. Therefore, it is necessary to carry out long-term investigations to see the impact of interventions on behavior change, including prevention and regularity of therapy, and reducing the prevalence of scabies. To prevent further transmission, adequate health education must begin when the patient is diagnosed with scabies at the Community Health Center, involving an interprofessional team such as doctors and pharmacists.
Peningkatan Nilai Ekonomis Madu melalui Pelatihan Pembuatan Fermented Garlic Honey di Panti Asuhan Al-Madina Surabaya Wulansari, Devyani Diah; Savitri, Emma; Kirtishanti, Aguslina; Wulandari, Devyana Dyah; Susilo, Raden Jokokuncoroningrat; Wuryaningsih, Lucia Endang
Wikrama Parahita : Jurnal Pengabdian Masyarakat Vol. 8 No. 2 (2024): November 2024
Publisher : Universitas Serang Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30656/jpmwp.v8i2.7405

Abstract

Berwirausaha merupakan salah satu upaya yang dapat dilakukan untuk membangkitkan kembali perekonomian Indonesia paska pandemi Covid 19. Untuk dapat mengembangkan usahanya sendiri masyarakat harus dapat melakukan kretifitas yang dapat membuat sesuatu yang baru, berbeda dan dapat mengembangkannya. Grha Aitam merupakan panti asuhan berbasis entrepreneur sebagai konsep pemberdayaan anak yatim dan para dhuafa, berbekal dukungan dari stake holder, khususnya donatur. Salah satu bentuk wirausaha yang ditekuni saat ini berupa pengemasan madu. Hasil dari penggalian informasi dengan mitra, konsumen baru yang ditawarkan madu rata-rata kurang tertarik dengan rasa dan bau madu, serta kurangnya wawasan terkait manfaat madu untuk kesehatan. Melalui program pengabdian kepada masyarakat, solusi yang ditawarkan oleh tim Pengusul kepada Mitra adalah membuat sebuah inovasi produk madu berupa Fermented Garlic Honey (FGH) yang menawarkan rasa dan bau lebih menarik serta unik, serta klaim manfaat kesehatan yang baik, dengan menggunakan aplikasi teknologi fermentasi sehingga dapat dihasilkan produk berkualitas. Program ini diawali dengan evaluasi proses pengemasan madu dengan meninjau kembali layout sarana yang digunakan, kemudian diberikan pengetahuan terkait proses pembuatan serta manfaat medis FGH. Hal ini didukung dengan pengadaan alat fermentasi hingga pada pengemasan serta pengujian laboratorium untuk menganalisis kualitas FGH yang dihasilkan. Melalui serangkaian pelatihan yang difasilitasi oleh Tim, mitra dapat menghasilkan produk FGH yang aman, berkualitas dan berdaya jual tinggi.
Olaparib As Therapy For Metastatic-Castration Resistant Prostate Cancer : Systematic Review And Meta Analysis Putra Gunawan, I Made Rian; Herawati, Fauna; Kirtishanti, Aguslina
Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Vol 11, No 1 (2024): February
Publisher : Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jfst.v11i1.5284

Abstract

Prostate cancer is the second most prevalent cancer in men with approximately 1.4 million men worldwide. The main therapy for prostate cancer is androgen deprivation therapy (ADT), but patients who have received ADT may experience a condition of castration resistant prostate cancer (CRPC). More than 84% of patients have metastasized when diagnosed with CRPC (mCRPC) and median survival about 36 months. The Food & Drugs Association (FDA) has approved a new therapy for mCRPC patients, an example is olaparib. The purpose of this systematic review and meta analysis is to assess effectiveness (overall survival) and safety of olaparib in mCRPC. This research is using randomized control trial’s (RCT) article. The literature search process was carried out using the PubMed database. The quality of inclusion was assessed using the Critical Appraisal Skill Program (CASP) checklist and journal reputation. The results of the meta-analysis on the effectiveness of olaparib has showed that there was no significant difference in the patient's overall survival rate (RR=0. 81; 95% CI=0.58-1.13). The results of the meta analysis on the safety level of olaparib has showed a significant difference, seen from the side effects such as anemia (RR=3.47; 95% CI=2.59-4.65), nausea (RR=2.05; 95% CI=1.62-2.60) and fatigue (RR=1.32; 95% CI=1.10-1.59). The conclusion is olaparib as mCRPC therapy does not show significant effectiveness in improving overall survival in mCRPC. In addition, the low safety level of olaparib in mCRPC patients were seen from side effects such as anemia, nausea and fatigue
Aktivitas Sitotoksik Ekstrak Biji Alpukat (Persea americana Mill.) pada Sel Kanker Payudara dan Serviks Secara In Silico dan In Vitro Kirtishanti, Aguslina; Kesuma, Dini; C, Fadita Trisa; Tuga, Maria Claudia Dwiyanti
MPI (Media Pharmaceutica Indonesiana) Vol. 4 No. 2 (2022): DECEMBER
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v4i2.5296

Abstract

Kanker payudara dan servik merupakan kanker dengan jumlah terbanyak di Indonesia. Kemoterapi sebagai terapi kanker memiliki banyak efek samping, oleh karena itu diperlukan pengembangan obat antikanker terutama dari bahan alam yang efektif dan memiliki efek samping minimal. Salah satu bahan alam yang diprediksi mempunyai aktivitas antikanker adalah biji alpukat (Persea americana Mill.). Penelitian ini bertujuan untuk menentukan aktivitas sitotoksik ekstrak etanol biji alpukat pada sel kanker payudara dan serviks secara in silico dan in vitro. Senyawa aktif dalam biji alpukat di docking dengan reseptor estrogen (PDB code: 3ERT) and reseptor SIRT1 (PDB code: 4I5I) menggunakan program Molegro Virtual Docker 5.5 (MVD). Aktivitas sitotoksik secara in vitro dilakukan menggunakan metode Microculture Tetrazolium Technique (MTT) pada sel kanker payudara (MCF7), sel kanker serviks (HeLa) dan sel normal (Vero). Biji alpukat berisi 10 senyawa aktif yang diprediksi mempunyai aktivitas sitotoksik. Hasil uji in silico menunjukkan bahwa epicatechin gallate mempunyai nilai rerank score paling rendah yaitu -118,397 kkal/mol pada reseptor estrogen dan -133,694 kkal/mol pada reseptor SIRT1. Aktivitas sitotoksik secara in vitro ditunjukkan dengan nilai IC50 sebesar 537,37 μg/mL (MCF7), 383,21 μg/mL (HeLa) dan 541,67 μg/mL (Vero). Dari hasil uji in vitro menyatakan bahwa ekstrak etanol biji alpukat tidak memiliki aktivitas sitotoksik pada sel kanker MCF7 dan memiliki aktivitas sitotoksik lemah pada sel HeLa. Breast and cervical cancer are cancers with the highest number in Indonesia. Chemotherapy, as one of the mainstay treatments of cancer, can cause harmful side effects; and, therefore, it is necessary to develop anticancer drug from natural ingredients with good efficacy and minimal side effects. One of the natural ingredients that is predicted to have anticancer activity is avocado seed (Persea americana Mill.). This study aimed to evaluate the in-vitro and in-silico cytotoxic activity of avocado seed extract on breast and cervical cancer cells. The active compounds in avocado seeds were docked with estrogen receptors (PDB code: 3ERT) and SIRT1 receptors (PDB code: 4I5I) using the MVD program. Cytotoxic activity in vitro was carried out using the MTT method on breast cancer cells (MCF7), cervical cancer cells (HeLa) and normal cells (Vero). Avocado seed contains 10 active compounds which are predicted to have cytotoxic activity. The findings from in-silico test showed that the “epicatechin gallate” had the lowest rerank score, i.e. -118.397 kcal/mol for the estrogen receptor and -133.694 kcal/ mol for the SIRT1 receptor. Cytotoxic activity in vitro was shown by IC50 values of 537.37 μg/mL (MCF7), 383.21 μg/mL (HeLa) and 541.67 μg/mL (Vero), respectively. The findings from in-vitro test showed that the avocado seed extract did not have cytotoxic activity on MCF7 cells and had weak cytotoxic activity on HeLa cells.
Potential of Red Ginger Rhizome (Zingiber officinale Roscoe var. Rubrum) as an Anti-Cancer: A Review Anggara, I Gede Yudi; Kirtishanti, Aguslina; Gondokesumo, Marisca E.
Pharmacology and Clinical Pharmacy Research Vol 9, No 3 (2024)
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15416/pcpr.v9i3.52562

Abstract

Red ginger rhizome, Zingiber officinale Roscoe var. Rubrum has long been used as a herbal medicine. Its biological properties, including anti-cancer properties, are confirmed. Compared to their stems and leaf tissues, the average amount of gingerols in red ginger rhizomes (104.39 μg/g) is much higher. In addition to gingerol, other nonvolatile pungent chemicals found in ginger include shogaols, paradols, gingerdiols, and zingerone. Gingerol disrupts the cell cycle, triggers apoptosis in cancer cells, and prevents the growth and spread of cells by interacting with particular proteins, gene targets, and signal transduction pathways. 6-shogaol activity suppresses NF-kB and signal transducer and activator of transcription 3 (STAT3), decreases Bcl-2 expression, and increases Bax expression, which contributes to lowering cell viability and causing autotosis. The several functions of red ginger in regulating biological processes linked to anticancer effects will be outlined in this review. In order to organize related study findings in accordance with the specified publication criteria, this literature review employs a narrative technique. The chemical composition of red ginger rhizome that functions as an anti-cancer agent contains 6-gingerol, 6-shogaol, 10-gingerol, 6-paradon, and zingeron, according to the findings of this literature review that was conducted. Red ginger rhizome has been proven to have anti-cancer activity in various types of cancer that have been studied in vitro and in silico, there are head and neck cancer, endometrial adenocarcinoma,breast cancer, lung cancer, skin cancer, colon cancer, prostate cancer, leukemia, oral and cervical cancer, brain tumor, bone cancer, and pancreatic cancer.
Implementation of Interprofessional Education in Medical and Pharmacy Students with Year Differences: A Case Study Wibowo, Ika Mulyono Putri; Setiawan, Eko; Wicaksono, Y. Adhimas Setyo; Kirtishanti, Aguslina; Dahliana, Anita; Susilo, Astrid Pratidna
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 14, No 1 (2025): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.95678

Abstract

Background: Interprofessional education (IPE) is the foundation to prepare students for the core competencies to be an effective member of a healthcare professional team. The core IPE competencies include values and ethics, roles and responsibilities, interprofessional communication, and teamwork. Challenges occur when attempting to adopt IPE in the existing curriculum. While reports of IPE involving students with the same academic years have been documented, little is known about the feasibility to conduct IPE for students with different academic years in the settings without formal IPE courses.Aims: To explore students’ experience in implementation of IPE with different health disciplines and academic years.Case Discussion: Sixth year medical students and fourth year pharmacy students were invited to an online discussion session to reflect their experiences while collaboratively working on two health promotion projects in a Community Health Center. In addition, all teachers of the project participated in the discussion. The discussion was recorded, transcribed, and further analysed. Students showed mutual respect for each profession's role, learned from each other, and collaborated in preparing materials and implementing activities. Extensive discussions with teachers are a key component of the implementation. Differences in academic year did not impede implementation.Conclusion: This case study shows that IPE involving students with different academic years is feasible without jeopardising the attainment of the core IPE competencies. Experiential learning through health promotion projects can be an effective approach for IPE in the settings where IPE is an add-on program in the existing curricular.